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Generic platform for colorimetric assay of melanocyte laden 3D hydrogel
곽호정,신성철,현진호 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
A colorimetric assay for melanogenesis inhibition is demonstrated with polyethylene glycol tetraacrylate (PEG-4A) hydrogels embedding melanocyte cells. The cell-laden three-dimensional (3D) PEG-4A hydrogel structure can be prepared by digital light processing (DLP) 3D printing using the visible range of light for improving biocompatibility. The melanocytes in the PEG-4A hydrogel show high cellular compatibility and expressed melanin, which changes the color of the hydrogel to black. Inhibition of melanin expression is evaluated by injecting different concentrations of arbutin and kojic acid into the cell-laden hydrogel structure in a 3D-printed microfluidic chip. It is demonstrated that the tyrosinase inhibitors prevent color change of the hydrogel pad and it can be used for an alternative monitoring tool of skin responsiveness in the future.
Silk fibroin-PEG hydrogel scaffold to construct human skin equivalent model using DLP 3D printing
곽호정,신성철,나경아,이현지,현진호 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0
The human skin equivalent (HSE) models are used as an alternative animal test of permeation and toxicity screening of chemicals. Here, we demonstrate digital light processing (DLP) three dimensional (3D) printing for the mimicry of the HSE model. In comparison with traditional methods for constructing HSE, it offers advantages in terms of cell laden retention, reproducibility and high culture throughput. Primary human keratinocytes are incubated on a silk fibroin-PEG hydrogel scaffold containing fibroblasts and also grown at the air-liquid interface, which allows the enhanced maturation and stratification. In the presentation, the detailed process of purification of silk fibroin from the cocoon, the synthesis of acrylated-PEG, 3D printing of bioinks, and the critical parameters of the cell culture will be provided.
곽호정,신성철,이현지,현진호 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.72 No.-
Silkfibroin (SF)-incorporated 4-arm polyethylene glycol acrylate (PEG4A) was used as a bioink for digitallight processing three-dimensional printing. A visible range of light was adopted for the process to assurethe safety of the cells in the bioink. Fibroblast cells were embedded in the printed hydrogels, and thekeratinocyte cells were cultured at the surfaces of the hydrogels. The proliferation of cells with the bioinkmaterials, PEG4A and SF-PEG4A, and the toxicity of these materials, were verified by live/dead andalamarBlue assays. The primary human keratinocytes on the SF-PEG4A hydrogel scaffold generated akeratin layer by the tissue culture at the air-liquid interface, promoting maturation and stratification. SFpolyethyleneglycol hydrogels showed higher cell proliferation and cell spreading, and the thicker keratinlayer was formed with SF-PEG4A hydrogels compared with PEG4A hydrogels.