http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
제주도 주목(Taxus cuspidata S. et Z.)의 Taxol 및 Cephalomannine의 함량분석
곽상수(Sang Soo Kwak),최명석(Myung Suk Choi),박용구(Kyung Soo Ko),고경수(Young goo Park),유장렬(Jang Ryol Liu) 한국생약학회 1995 생약학회지 Vol.26 No.2
To seek for a stable supply source of taxol as an anticancer agent urgently needed for the treatment of ovarian cancer, the concentrations of taxol and cephalo mannine in bark and needles of 37 yew (Taxus cuspidata S. et Z.) trees grown in 6 locations of Cheju Island were analyzed by using HPLC. A considerable variation in the content was observed in plant part and the location. The average taxol content in the needles (220.3 ± 56 ㎍ per g dry wt) was ca. 4 times hgher than that in the bark, whereas the content of cephalomannine in the needles (353 ± 141 ㎍) was ca. 5 times higher than that in the bark. Particularly, even within Cheju Island, needles from trees grown near the coast including Sogwipo area contained much higher taxol contents than needles from trees grown at mountain locations, indicating that taxane level is affected by environmental effects. Taxol in the needles was confirmed by electrospray mass spectrometry. These results suggest that the needles of yew trees growing in Cheju Island may provide a renewable source for taxol.
산업디자인 지적재산권 출원의 정량적 판단 기준을 위한 디자인 선행조사 방안에 관한 연구
곽상수 ( Sang Soo¸ Kwak ) 한국지식재산연구원 2009 지식재산연구 Vol.4 No.2
The purpose of this study is to suggest the way to activate design database of intellectual property rights to help a designer at a company collect information for design development and intellectual property rights. This study presents the method of using data and services easily and accurately when design rights are registered through introducing the design information of systems built by governments or public organizations, or general design database information. This study also compares industrial design with intellectual property rights, and analyzes the method of collecting design information in the design database of intellectual property rights and general design database. Moreover on demand service and on-line service at a company or a public organization of each country including Japan and other major countries is analyzed on the basis of the design information. Therefore, this study suggests the method of design prior search to maximize the use of design information practically and presents the utilized examples.
Naphthoquinone류 화합물 흡수에 의한 페튜니아 배양세포내의 Phytoalexin 유도
김명조,곽상수 ( Myong Jo Kim,Sang Soo Kwak ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.4
To induce the phytoalexins in plant cell culture systems, we surveyed the antimicrobial activity following the feeding of five naphthoquinones in cell cultures of petunia. Among naphthoquinones treated, 2,5,7-trihydroxy-3-(5`-hydroxyhexyl)-1,4-naphthoquinone (3-OH NQ) was most efficiently absorbed into the cells within 48 hr. The crude extracts of cells treated with 3-OH NQ showed a strong inhibition activity on spore germination of Aspergillus candidus (MIC: 32 ㎍/㎖), whereas the untreated cells showed no activity. The two active compounds, 4,2`,4`,β-tetrahydroxychalcone and 4`,7-dihydroxyflavone, were isolated from petunia cells treated with 3-OH NQ. The major phytoalexin, 4,2`,4`,β-tetrahydroxychalcone, inhibited strongly the spore germination of A. candidus (MIC: 16 ㎍/㎖).
김수경,곽상수,정경희,민성란,박일현,유장렬 ( Soo Kyung Kim,Sang Soo Kwak,Kyung Hee Jung,Sung Ran Min,Il Hyun Park,Jang R. Liu ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.2
To establish an efficient system for peroxidase (POD) production by plant cell culture, we investigated the POD activity in 41 cell lines derived from 26 plant species using pyrogallol as substrate. A cell line from sweet potato (Ipomoea batatos cv. White Star) shoot meristem explants cultured on LS medium supplemented with 1 ㎎/ℓ 2,4-D showed the highest activity: 3,500 U/g cell dry wt, about 15 folds higher than that of intact roots of horseradish plants. Furthermore, a cell tine SP-47 having a high growth rate and POD yield was selected by a small cell-aggregate method, of which productivity was about 1.5 times higher than the original cell line in cell suspention culture. A hairy root culture obtained from sweet potato leaf tissue following infection with Agrobacterium rhizogenes strain 15834 also produced a high level of POD: 1,400 U/g dry wt. POD isoenzyme of the cell line and hairy roots of sweet potato showed the same patterns as those of the intacts roots. However, they were prominently different from those of intact roots of horseradish. One major isoenzyme of POD in SP-47 and hairy root covered about 90% of total POD. Due to its high productivity and dominant major isoenzyme, the cell culture system of sweet potato established in this study seems to be suitable for the mass production of POD.
두 가지 항산화유전자를 동시에 발현시킨 형질전환 톨 페스큐 식물체의 환경스트레스에 대한 내성 특성 해명
이상훈(Sang-Hoon Lee),이기원(Ki-Won Lee),김기용(Ki-Yong Kim),최기준(Gi Jun Choi),서성(Sung Seo),곽상수(Sang-Soo Kwak),권석윤(Suk-Yoon Kwon),윤대진(Dae-Jin Yun),이병현(Byung-Hyun Lee) 韓國草地學會 2007 한국초지조사료학회지 Vol.27 No.2
환경스트레스에 강한 내성을 지닌 신품종 톨페스큐를 개발할 목적으로 산화스트레스에 의해 강하게 유도되는 SWPA2 promoter 하류에 CuZnSOD와 APX 유전자가 엽록체에 동시에 발현하도록 제작한 벡터를 Agrobacterium법을 이용하여 톨 페스큐에 도입하였다. Hygromycin이 첨가된 선발배지에서 내성을 가지며 재분화된 형질전환 식물체를 pot로 이식하여 기내 순화 시킨 후, Southern 분석을 실시하여 본 결과, 발현벡터의 T-DNA 영역이 형질전환 식물체의 genome에 성공적으로 도입되었음을 확인하였다. 형질전환 식물체 잎 절편을 산화스트레스와 중금속을 포함하고 있는 용액에 처리하여 엽록체의 손상정도를 조사한 결과, 비형질 전환체에 비해 형질전환체는 강한 내성을 나타내었다. 또한 유식물체 수준에서 MV를 처리하여 내성을 비교한 결과, 비형질전환체에 비해 형질전환체는 손상을 덜 받았다. 이와 같은 연구결과는 CuZnSOD와 APX 유전자를 엽록체에 동시발현시키는 기술이 다양한 환경스트레스에 대해 복합재해내성을 가지는 다양한 작물을 개발하는데 유용하게 이용될 수 있음을 나타낸 결과이다. Environmental stress is the major limiting factor in plant productivity. As an effort to solve the global food and environmental problems using the plant biotechnology, we have developed transgenic tall fescue (Festuca arundinacea Schreb.) plants via Agrobacterium-mediated gene transfer method. To develop transgenic tall fescue plants with enhanced tolerance to the environmental stresses, both CuZn superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) genes were incorporated in a pIG121 binary vector and the both of the genes were controlled separately by an oxidative stress-inducible sweet potato peroxidase 2 (SWPA2) promoter expressed in chloroplasts. Leaf discs of transgenic plants showed 10-30% less damage compared to the wild-type when they exposed to a wide range of environmental stresses including methyl viologen (MV), H₂O₂ and heavy metals. In addition, when 200 ?M MV was sprayed onto the whole plants, transgenic plants showed a significant reduction of visible damage compared to wild-type plants that were almost damaged. These results suggest that over expression of CuZnSOD and APX genes in transgenic plants might be a useful strategy to protect the crops against a wide range of environmental stresses.