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몽골 유래 Brucella melitensis 동정 및 특이 SNP를 이용한 real-time PCR법에 의한 진단 평가
강성일,김지연,김숙미,이진주,성소라,김연희,정석찬,허문,Kang, Sung-Il,Kim, Ji-Yeon,Kim, Suk Mi,Lee, Jin Ju,Sung, So-Ra,Kim, Yeon-Hee,Jung, Suk Chan,Her, Moon 대한수의학회 2015 大韓獸醫學會誌 Vol.55 No.2
A real-time PCR assay using hybridization probe (HybProbe) has been developed to detect Brucella (B.) melitensis strains. The primer and HybProbe sets were designed based on the gap gene of chromosome I with a specific single nucleotide polymorphism of B. melitensis. Specificity of the assay was confirmed by comparison to reference Brucella species and other related strains. In the melting curve analysis, B. melitensis generated a peak at $67^{\circ}C$ unlike those for other Brucella species observed at $61^{\circ}C$. Sensitivity of the assay for B. melitensis ranged from 20 ng to 200 fg of genomic DNA. The ability to identify 94 Mongolian B. melitensis isolates using the real-time PCR assay was identical to that of classical biotyping methods and differential multiplex PCR. These data showed that this new molecular technique is a simple and quick method for detecting B. melitensis, which will be important for the control and prevention of brucellosis.
강성일(Sung Il Kang),강봉수(Bong Su Kang),안창욱(Chang Wook Ahn),고현석(Hyun Seok Ko),이성배(Sung Bae Lee),구혜영(Hye Young Koo),정철운(Chul Woon Chung) 한국간담췌외과학회 2008 한국간담췌외과학회지 Vol.12 No.3
A 57-year-old man with severe abdominal pain was admitted to our hospital. Chest PA and simple abdominal X-ray revealed no specific findings, but the abdominal-pelvis CT scan showed a 5cm sized multiloculated cystic tumor originating from the pancreatic head and a 3.2cm sized hepatic lesion that was suspected to be a metastic lesion. A radical operation was not able to be performed because of peritoneal metastasis and gastrocolonal infiltration. Only open lymph node biopsy was done and it revealed metastatic small cell carcinoma. Long-acting octreotide and gemcitabine was administered to the patient, but there was no therapeutic response. The tumor grew very rapidly to 26cm in size and the patient died 2 months later from his first hospital day. Necropsy was performed, and the pathologic finding of the resected mass was confirmed to be small cell carcinoma, the same as the result of the previous lymph node biopsy.
이중목적어(二重目的語)의 통어론적(統語論的) 기능고(機能考) -서기15세기 국어를 중심(中心)으로-
강성일 ( Sung Il Kang ) 東亞大學校附設 石堂傳統文化硏究院 1981 石堂論叢 Vol.5 No.-
This paper deals with the functions of two objectives appearing in simple sentences in the 15th century Korean language. The writer of this paper found that sentences with two objectives could be classified into the following four types. The 1st type is a variation of a dative as shown in the example 1-3, or one objective functioning in two ways. From the world-order of the surface structure alone, it was hard to tell whether the object was direct or indirect, for there were cases where an indirect object functioning as a dative came near to the predicative verb. In a sentence with two objectives, the verbs belonging to the "chu-da" ("give") group most clearly determine which one of them is a dative as in English. Therefore, the writer wants to call this sort of verb as the "dative verb" the objective governed by one of these verbs a "dative Objective". The 2nd type is, as shown in the example 5-6, a variation of an adjective and objective. This phenomena is prominently observable in a sentence where the verb belonging to the "nid-da" group. ("forget") governs the objectives. Likewise, the writer wants to term the verb as "genitive verb", the objective governed by such a verb as "genitive objective". The 3rd type is, as shown in the example 7-9, an objective functioning in two ways: instrumental case and objective. Therefore, the writer should like to term the verb as "instrumental verb", the objectives governed by this verb as instrumental objective. The group of verbs belonging to "sam-da"(make) comes under this category. The 4th type is, as shown in the example 10-12, a variation functioning in two ways: locative and objective. The characteristics of this locative is that a certain action is caused either within the limitation of a certain sphere or with the limitation of a certain period, drawn materially or non-materially. Therefore, the writer expects it proper to term this objective as locative objective. And the verbs(the group of verbs belonging to "machi-ta" (hit) verb) that functionalize the objectives in that way, could be termed as "locative verb".
강성일(Sung Il Kang),김종우(Jong Woo Kim),김승기(Seung Ki Kim),정철운(Chul Woon Chung),권성원(Sung Won Kwon),안대호(Dae Ho Ahn),이경포(Kyong Po Lee),이경식(Kyong Sik Lee) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.6
Purpose: Endometriosis is the heterotropic occurrence of island of endometrial glands and stroma outside of uterus. It common occurs in pelvic cavity, but rarely at the ectopic area of extra-pelvic cavity. The aim of this study was to evaluate the clinical characteristics of extrapelvic endometriosis in order to help diagnosis and treatment. Methods: Between January 2001 and June 2007, 22 patients who underwent surgery and was diagnosed as ectopic endometriosis of extra-pelvic cavity at Bundang CHA hospital were retrospectively reviewed with medical records and a telephone interview. Results: All cases were women in their reproductive age, with a median age of 33 years (range 24~49 years). 17 of 22 cases were endometriosis in the scarring tissue of prior caesarean section wound on abdomen. 1 case in vaginal orifice on episiotomy wound of perineum, 1 case in femoral ring area and 3 cases in appendix. All patients except endometriosis of appendix, were presented with mass, pain and symptoms almost associated with menstruation. 18 of 22 cases were suspected of having extra-pelvic endometriosis due to their specific clinical features, 4 cases were suspected of hernia and acute appendicitis. All patients were treated with surgery. None has revisited to clinics due to recurrence. Conclusion: Women who have mass or lump in surgical scar and symptoms associated with menstruation period should be suspected of having endometriosis. Endometriosis of surgical scaring tissue should be treated by wide local excision to prevent local recurrence.
복강경하 위소매절제술 후 합병증의 조기 발견에 있어 임상 지표의 중요성
강성일(Sung Il Kang),한상문(Sang Moon Han),김원우(Won Woo Kim),이경포(Kyung Po Lee),이경식(Kyung Sik Lee) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.5
Purpose: The significant drastic complications of performing laparoscopic sleeve gastrectomy (LSG) in morbidly obese patients are gastric staple line leakage and bleeding. The aim of our study is to evaluate the efficacy of the clinical data for detecting postoperative complications after LSG. Methods: The study enrolled 150 consecutive patients who underwent LSG from January 2003 to July 2006. When abnormal data (heart rate ≥110/min, or temperature ≥37.5℃) was detected on postoperative day 1, then laboratory tests (blood, urine, chest X-ray and abdominal sonogram) and water soluble gastrografin UGIS were performed to detect the postoperative complications after LSG (group A). The patients who had normal postoperative clinical data (group B) were compared with group A. Results: Of the 150 patients who underwent LSG, 9 patients (6%) had postoperative complications. Two patients had major complications: 1 case of leakage (0.6%) and 1 case of delayed bleeding (0.6%), and 4 patients had minor complications in group A. But no major complications were detected in group B (P<0.01). The heart rate and body temperature in group A were significantly faster and higher than those in group B (P<0.01). Especially, 2 patients who had major complications had severe tachycardia over 120 beats per minute (bpm). However, when the body temperature of a patient was elevated, there was no high grade fever in all the cases (>39℃). Conclusion: Evidence of tachycardia or a high body temperature may be useful to detect major complications after LSG. We also recommend performing laboratory test and UGIS when clinically indicated.