키토산올리고당을 섭취한 쥐에서 간 미세구조의 연구

김영호,노영복,Kim, Young-Ho,Roh, Young -Bok 한국현미경학회 2001 Applied microscopy Vol.31 No.4

본 연구의 목적은 키토산올리고당의 쥐 간조직 독성여부를 관찰하고자 하였다. 건강한 Wistar계 쥐를 사용하였다. 실험군은 Group 1. 일반식이를 섭취한 대조군, Group 2 0.1%(1mg/ml)키토산올리고당 수용액을 30일 간 음용수를 통해 자유자재로 섭취시킨 후 교미시켜 태어난 $F_1$ 세대, Group 3 $F_1$ 세대의 쥐에게 0.1%(1 mg/ml) 키토산올리고당 수용액을 30일간 음용수를 통해 자유자재로 섭취한 후 교미시켜 태어난 $F_2$ 세대, Group 40.1% (1 mg/ml) 키토산올리고당 수용액을 90일간 음용수를 통해 자유자재로 섭취시킨 군, Group 50.1% (1 mg/ml) 키토산올리고당 수용액을 365일간 음용수를 통해 자유자재로 섭취시킨 군의 쥐 등으로 각 실험군 당 쥐 10마리를 사용하였고, 다음과 같은 결과를 얻었다. Group 4의 경우 약간의 소포체 팽창을 관찰하였을뿐, 다른 실험군에서는 대조군과 비교하여 특별한 간 조직의 미세구조 변화를 관찰하지 못하였다. 결론적으로 키토산올리고당은 무독성 물질로서 안전성이 있다고 사료된다. The purpose of this study was to evaluate the histological toxicity of chitosanoligosaccharide on the rat. A healthy male of Wistar rat that weighted $250{\pm}350g$ was used for experiment The experimental group was divided into five groups. Group 1 was control group which treated with general food Group 2 was $F_1$ generation which was born by mating of 0.1% (1 mg/ml) chitosanoligosaccharide was supplied by feeding ad libitum for 30 days. Group 3 was $F_2$ generation which was born by mating $F_1$ generation. Group 4 was treated with 90 days of 0.1% (1 mg/ml) chitosan oligosaccharide. Group 5 was treated with 365 days of 0.1% (1 mg/ml) chitosanoligo saccharide. All experimental groups were used to 10 rat. The results were as follow: The RER dilation was observed Group 4. However, there were no significantly changes of ultrastructures in the other groups compared to the control. It was concluded that chitosanoligosaccharide can be used for nontoxic natural material.

냉동장치용 열교환기의 성능 향상(II)

김영호,Kim, Young Ho 대한설비공학회 1988 설비저널 Vol.17 No.1

식품산업에서의 파동에너지의 재조명

김영호,이남혁,Kim, Young-Ho,Lee, Nam-Hyouk 한국식품연구원 2009 食品技術 Vol.22 No.3

로그기반 상황인식에 의한 소규모 창고관리시스템

김영호,최병용,전병환,Kim, Young-Ho,Choi, Byoung-Yong,Jun, Byung-Hwan 한국정보처리학회 2006 정보처리학회논문지B Vol.13 No.5

유비쿼터스 컴퓨팅의 일환으로 무선인식(RFID)을 이용한 많은 응용시스템이 개발되고 있으며, 특히 물류산업에 RFID 칩이 널리 보급될 것으로 예측된다 중소업체의 경우 소규모 창고관리에도 비용과 인력 측면에서 어려움을 격고 있는 상황에서 효율적이고 정확한 지능형 창고관리 시스템의 필요성이 절실하다. 본 논문에서는 로그기반 상황인식 기술을 이용하여 소규모 창고관리시스템을 구현한다. RFID 리더기를 제어하는 클라이언트를 구성하고 서버에 DBMS 시스템을 구축하여 웹상에서 운영되는 창고관리시스템을 구현한다. 특히, 시스템 접근 이력에 대한 로그데이터를 토대로 사용자의 입출고 의도를 파악하며, 관리자에게 사용자의 비정규적인 창고사용 패턴을 보고하고 예측 가능한 재고관리 서비스를 제공한다. 결과적으로, 제안한 시스템은 소규모 창고관리의 효율성과 정확도를 향상시키는데 기여할 수 있다. Various application systems are developed using RFID as a part of ubiquitous computing, and it is expected that RFID chip will become wide-spread for the distribution industry especially. Efficient and efact intelligent-type of warehouse management system is essential for small-to-medium-sized enterprises in the situation having a trouble in the viewpoint of expense and manpower. In this paper, we implement small-scale warehouse management system using log-based context awareness technology. This system is implemented to be controlled on web, configuring clients to control RFID readers and building up DBMS system in a server. Especially, it grasps user's intention of storing or delivering based on toE data for the history of user's access to the system and it reports user's irregular pattern of warehouse use and serves predictive information of the control of goods in stock. As a result, the proposed system can contribute to enhance efficiency and correctness of small-scale warehouse management.

Rat에서 뇨중 ${\gamma}$-Glutamyltranspeptidase와 N-Acetyl-$\beta$-D-glucosaminidase 측정에 의한 신독성 평가에 관하여

김영호,이창우,Kim Young-Ho,Lee Chang-Woo 한국임상수의학회 1990 한국임상수의학회지 Vol.7 No.2

Present experiment was performed in order to establish the optimum conditions for quantitation of ${\gamma}$-GTP and AGS activities in rat urine and investigate the applicability of the these enzymes in experimental assessment of nephrotoxicity in rats. The results obtained were as follows. 1. The optimal pH of Tris-BCI buffer containing glycylglycine for determination of urinary ${\gamma}$-GTP activity was 7.6(37$^{\circ}C$). 2. The Michaelis constant of urinary ${\gamma}$-GTP ranged from 1.1 to 1.2 mmol/$\ell$. 3. The optimal pH of citrate buffer for determination of urinary AGS activity was 3.6(37$^{\circ}C$). 4. The Michaelis constant of urinary AGS ranged from 0.8 to 0.9mmo1/$\ell$. 5. Coefficient of variance for within-run imprecision of urinary ${\gamma}$-GTP ranged from 3.8 to 6.4% and that of urinary AGS ranged from 2.5 to 4.1%. 6. There was no significant difference between gel-filtered samples and crude samples in the mean activity of urinary ${\gamma}$-GTP and the intra-individual differences by gel-filtration were either increased or decreased. Mean values of ${\gamma}$ -GTP activities in gel-filtered samples and crude samples were 1570 and 1590 U/$\ell$, repectively. 7. The mean activity of urinary AGS increased significantly after gel-filtration and all the individual urines revealed higher activities after gel-filtration. 8. ${\gamma}$-GTP and AGS activities were linear to 135 and 7U/$\ell$, respectively. 9. Urinary ${\gamma}$-GTP and AGS excretion before administration of potassium dichromate were 22.1 ${\pm}$ 11.2 and 0.5${\pm}$0.2 U/24hrsㆍkg body weight respectively and increased significantly to 102.3${\pm}$44.5 and 5.8${\pm}$3.30/24hrsㆍkg body weight respectively within 24 hours after administration. 10. BUN increased continuously from 24 hours following exposure to potassium dichromate in all 10 rats. From these findings it is concluded that the urinary ${\gamma}$-GTP and AGS excretions are early and sensitive indicators for nephrotoxicity assessment in rat.

감국의 품질 평가

김영호,민병선,정현주,하티탄훵,이준성,지형준,배기환,Kim, Young-Ho,Min, Byung-Sun,Jung, Hyun-Ju,Lee, Jun-Sung,Chi, Hyung-Joon,Bae, Ki-Hwan 한국생약학회 1999 생약학회지 Vol.30 No.1

The flower of Chrysanthemum morifolium L. has been used as 'Kamguk' in the Korean markets instead of it from C. indicum L. In order to evaluate the quality of Chrysanthemi Flos, the method for isolation and quantitative determination of luteolin as standard compound has been developed. It is analyzed with HPLC using the solvent system of MeOH-water-acetic acid (30:70:5). The amounts of luteolin from Chrysanthemi Flos in Korean markets are in the range of $0.03{\sim}0.04%.$ The amounts of luteolin from the flowers of C. indicum and C. boreale are approximately 0.14% and 0.04%, respectively.

The Elements of the Bernesque Satire in Byron's Poetry

Kim, Young Ho김영호 崇實大學校 大學院 1987 崇實大學校 大學院 論文集 Vol.5 No.-

세포내 정보전달 저해물질

김영호,노재섭,Kim, Young-Ho,Ro, Jai-Sup 한국생약학회 1992 생약학회지 Vol.23 No.2

용융 시간에 따른 PTT/PET 용융 블렌드의 열적 특성 변화

김영호,방경,Kim Young Ho,Pang Kyeong 한국섬유공학회 2004 한국섬유공학회지 Vol.41 No.6

Melt blends of poly(trimethylene terephthalate) (PTT) and poly(ethylene terephthalate) (PET) of various ratios were prepared by mixing at $280^{\circ}C$ for different times using twin screw type internal mixer, and their thermal properties were investigated by DSC, DMA and NMR spectroscopy. With increase in mixing time at $280^{\circ}C$, the two melting peaks of PTT/PET blends, corresponding to the melting of PTT and PET, merged to one peak and moved to lower temperatures, while the crystallization temperature moved to higher temperatures. DMA analysis indicated a single glass transition temperature for the PTT/PET melt blends of various compositions prepared by mixing at $280^{\circ}C$ for 5 minutes, indicating that the blends are completely miscible in the amorphous region. The transesterification reaction in the PTT/PET melt blends was confirmed bi NMR spectroscopic analysis. Degree of randomness and sequence length of the blends were calculated by using $^13C-NMR$ data. The former increased while the latter decreased as the melt mixing time increased.

수용성카이토산에 의한 마우스태반을 통한 태아로의 방사성스트론튬 급성 전이 억제

김영호,범희승,김지열,Kim, Young-Ho,Bom, Hee-Seung,Kim, Ji-Yeul 대한방사선방어학회 1997 방사선방어학회지 Vol.22 No.4

카이토산은 천연무독성 착화제의 일종으로 유전적 독성이 거의 없으며, 방사성스트론튬의 마우스 체내 축적을 감소시킨다고 알려져 왔다. 본 연구에서는 임신 17일째 마우스에서 방사성스트론튬 오염전 후에 수용성카이토산을 공급함으로써 오염 후 태반을 통한 태아로의 방사성스트론튬 급성 전이를 얼마나 효과적으로 억제할 수 있는 가를 알아보고자 하였다. 수용성카이토산 분말을 50일간 일반식이와 혼합하여 10% (Group 1), 1%(Group 2)를 공급하고 임신 17일째에 방사성스트론튬을 오염시킨 군의 경우 일반식이를 공급한 대조군과 방사성스트론튬 오염 후 카이토산 10%, 1%분말을 공급한 군(Group 3, Group 4)에 비해 태반을 통한 태아로의 방사성스트론튬 전이를 효과적으로 억제함을 관찰하였다(p<0.01, 표 1).본 실험을 통해서 임신 17일째에 방사성스트론튬에 오염된 임신마우스의 경우 천연무독성 착화제인 카이토산을 장기간 전처치하였을 경우 태반을 통한 태아로의 방사성스트론튬 전이를 억제할 수 있음을 알 수 있었다. It has been reported that chitosan has little genetic toxicity as one of natural and nontoxic chelator and reduces the internal retention of radiostrontium in the mouse. This study is to examine that when water soluble chitosan is provided to the mouse on 17 days of pregnancy before and after radiostrontium contamination, how effectively it can inhibit an acute transfer of radiostrontium to fetus through placenta contaminated. Water soluble chitosan powder is mixed with general food for 60 days and 10%(Group 1) and 1%(Group 2) are provided respectively, and it is observed that the group with radiostrontium contamination on 17 days of pregnancy can inhibit more effectively the transfer of radiostrontium to fetus through placenta than control group with general food and the groups (Group 3, Group 4) with 10% and 1% of chitosan powder respectively after radiostrontium contamination (p<0.01, Table 1). It is found that when the pregnant mouse contaminated by radiostrontium on 17 days of pregnancy is prefed by chitosan, the transfer of radiostrontium to fetus through placenta can be inhibited.