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Sodium Nitrite와 L-Arginine과의 反應 및 反應生成物의 突然變異原性에 關한 硏究
鄭圭燦,鄭光元,張億奎 영남대학교 자원문제연구소 1985 資源問題硏究 Vol.4 No.-
Nitrates and nitrites are used as preservatives and coloring agents in food products, especially cured meats. A number of materials including secondary or tertiary amines or amides in their structure may interact with nitrite to form N-nitroso compounds which are known as chemical carcinogen or Mutagen. Food, drugs, cosmetics, agricultural chemicals (e. g., pesticides), tobacco and certain occupational settings are significant sources of nitrosatable amides and amines, Amino compounds that may interact with nitrite to form N-nitroso compounds are present in the environment. In this study, we have tested L-Arginine, one of the amino acids. L-Arginine was reacted with nitrite at acidic conditions and reaction products were purified and analyzed by means of Thin-Layer Chromatograpy, Infrared Absorption Spectroscopy and Nuclear Magnetic Reso-nance Spectroscopy. Studies about it's mutagenicity, DNA damage were tested by "Rec-assay".
인삼 Ether추출물과 Selinium이 7,12-Dimethylbenz(a)anthracene의 돌연변이원성에 미치는 영향
정규찬,강규모,남경수,Chung, Kyu-Cham,Kang, Gyu-Mo,Nam, Kyung-Soo 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.2
DMBA의 대사과정에 관련하는 효소와 체내 방어기전에 인삼 ether추출물과 selenium이 어떤 영향을 미쳐 돌연변이가 억제되는지 알아보기 위해 인삼 (2 mg/kg)과 selenium ($5\;{\mu}mol/kg$)을 10일간 흰 쥐에 투여하였다. 이때, GST와 GSH-px활성은 각각 12.3%, 186% 증가하였으며 DMBA-induced lipoperoxide 형성도 억제되었다. Ames 방법으로 DMBA ($205\;{\mu}g/plate$)의 돌연변이원성에 미치는 영향을 조사한 결과, 인삼투여군은 17%, selenium투여군은 22%, 동시투여군은 41%까지 억제하였으며 시험관 내에서 인삼농도가 0.5~2 mg/plate일때 14~38%, selenium 농도가 $1{\sim}4\;{\mu}mol/plate$일 때 32~66% 억제됨을 알 수 있었다. Glutathione S-transferase and glutathione peroxidase are a group of multifunctional enzymes that involved in the metabolic conversion of DMBA. When ginseng ether extracts (2 mg/kg, i.p., 10 days) and selenium ($5\;{\mu}mol/kg$, i.p., 10 days) to rats, GST and GSH-px activities showed by 12.3%, 186% as compared with the normal group respectively. Also, this materials inhibited on the formation of DMBA-induced lipoperoxide. Antimutagenic effects of ginseng ether extracts, selenium, and ginseng+selenium in the DMBA ($20\;{\mu}g/plate$) inducing mutagenicity using Salmonella typhimurium strain TA 98 by the Ames method exhibited by 17%, 22%, and 41%, respectively. Direct additions of ginseng ether extracts (0.5~2 mg/plate) and selenium ($1{\sim}4\;{\mu}mol/plate$) with S-9 mixture to the mutagenicity assay system showed 14~38%, 32~66% inhibition of DMBA mutagenicity, respectively.
정규찬,백석환,남경수,Chung, Kyu-Charn,Baek, Suk-Hwan,Nam, Kyung-Soo 대한약학회 1988 약학회지 Vol.32 No.1
Effects of acanthopanax cortex extracts on glutathion S-transferase (GST), glutathion peroxidase (GSH-px) and superoxide dismutase (SOD) activities related to 7,12-dimethyl-benz(a)anthracene(DMBA) metabolism and on DMBA-induced mutagenicity were investigated in this study. From the comparative study of three extracts, it was found that butanol extract was more potent than other extracts in increment of GST, GSH-px and SOD activities and in inhibitory effects of lipoperoxide formation of liver. Also ether and butanol extracts inhibited DMBA-induced mutagenicity, showing 33% to 36% of inhibition at maximum, when ether and butanol extracts were administered to rats intraperitoneally.
마늘 Allicin分割과 水溶性 抽出物이 7,12-dimethylbenz(α)anthracene의 突然變異原性에 미치는 影響
鄭圭燦,方明圭,金聖煥 영남대학교 자원문제연구소 1989 資源問題硏究 Vol.8 No.-
Many chemical carcinogens are biologically inactive as parent compounds but can be activated by microsomal enzyme to electrophilic metabolites that may react with the celluar nucleophiles to produce biological effects. Many dietary components have been shown to profoundly influence the activities of the microsomal enzyme system involved in the activation and detoxification of xenobiotics. In these studies, we have observed that the effect of allicin fraction and garlic aqueous extract on glutathione s-transferase (GST) and glutathione peroxidase (GSH-px) activities involved in 7,12-dimethylbenz(a)anthracene (DMBA) metabolism and the induced mutagenicity.
쇠뜨기(Equisetum arvense L.) 成分의 抗突然變異原性에 관한 硏究
정규찬,김미정 영남대학교 지역발전연구소 1995 새마을지역개발연구 Vol.17 No.-
This study was attempted to investigate the antimutagenic effect of Equisetum arvense L. The sterile stems of Equisetum arvense L. were refluxed with MoOH. The dryed MeOH fraction were partitioned with hexane, CHC1, and EtOAc successively. When carried out Ames test with Samonella typhomurium strain, it showed that all extracted fractions had antimutagenicity activity in TA 98 treated by NPD as well as TA 100 by NaN₃. Among them, EtOAc fraction had the most potent antimutagenicity. In the comparative study of inhibition for rat liver microsomal lipid peroxidation by each fractions, EtOAc fraction has inhibited lipid peroxidation most strongly. Also EtOAc fraction has been highly flavonoid contents. This effect was correlated with flavonoid contents of each frations.
鄭圭燦,白錫煥,權東烈,申恒淳 영남대학교 자원문제연구소 1988 資源問題硏究 Vol.7 No.-
This study was designed to compare the effect of antioxidants (BHA, BHT, Vitamin C and Vitamin E) on hepatic enzyme activites (glutathione S-transferase, glutathione peroxidase, superoxide dismutase) to the inhibitory effect of antioxidants on lipoperoxide formation. We used mice as experimental animals for the subject of in vivo. When the enzyme activities in liver was tested after acute paracetamol (500mg/kg, i.p.) intoxication, it was decreased in comparison to control. But the enzyme activities after antioxidants (0.5% W/W, 10 days treatment), it was increased than control group. Also, from the comparative study of four antioxidants, it was showed that synthetic antioxidants (BHA, BHT) were more potent than vitamins (vitamin C, vitamin E) in increment of glutathione S-transferase, glutathione peroxidase activities. But synthetic antioxidants and vitamins were showed to have a similar to increment of superoxide dismutase activity and to inhibitory effect of lipoperoxide formation of liver.
亞窒酸鹽과 Sulfa 劑와의 反應 및 反應生成物의 突然變異原性 硏究
鄭圭燦,方永俊,白錫煥,南景琇 영남대학교 자원문제연구소 1987 資源問題硏究 Vol.6 No.-
Nitroso compounds (especially N-nitroso compounds) are known to be widely distributed in the human environments and can also be formed in the human body. There have been many reports about toxicity of nitroso compounds in view of human cancers. Therefore, it is important and imminent for us to monitor nitroso compounds in environmental substances such as foods, cosmetics and drugs. We have tested sulfamethoxazole and sulfisoxazole that are widely used as antibacterial sulfa drugs. Sulfamethoxazole and sulfisoxazole easily reacted with nitrite at acidic conditions and reaction products were purified and mutagenicity of reaction products were tested by Ames method.
亞窒酸鹽과 아미노酸에 依한 N-nitroso 化合物의 突然變異原性
鄭圭燦 嶺南大學校 環境問題硏究所 1987 環境硏究 Vol.7 No.2
The reactivities between sodium nitrite and amino acids in acidic conditions and the mutagenicities of the reaction products were studied. N-nitroso compounds were easily formed by the reactions of amino acids with sodium nitrite in acidic conditions at 36.5℃. The formation of N-nitroso compounds increased with acidity and was proportional to the concentration of sodium nitrite and amino acids within some limits. Sodium citrate and tartrate accelerated the formation of N-nitroso compounds, while ascorbic acid markedly inhibited the reaction. The reaction products showed DNA damaging effects on Bacillus subtilis as determined by "Rec-assay" method. Ames' test using Salmonella Typhimurium TA series showed that the reaction products were frameshift type mutagens.