흰쥐 뇌의 푸로스타글란딘 D_2 (PGD_2), E_2 (PGE_2) 및 F_2α (PGF_2α)의 함량

朴詩苑 상명대학교 논문집 1986 상명대학교논문집 Vol.18 No.-

The basal concentrations of three major prostaglandins (PG) were determined by the very sensitive radioimmunoassay (RIA). When rat was sacrificd by microwave irradiation, the values of PGD_2, PGE_2 and PGF_2α were 1.35±0.22, 0.74±0.17 and 0.27±0.13 ng/ whole brain respectively, whereas other methods like simple decapitation, perfusion or perfusion with indomethacine showed far higher levels of the corresponding prostaglandins. In order to investigate the basal concentrations of the prostaglandins in any specific part of the brain, rat brain was dissected into 13 parts. PGD_2 was the most significant in pineal gland (38.4±9.6 ng/g wet weight) and posterior pituitary (17.8±4.1 ng/g wet weight). On the other hand, PGE_2 was found to be high in hypothalamus(14.8±0.9 ng/g wet weight), and anterior and posterior pituitary (18.2±0.4 and 17.3±2.7 ng/g wet weight). The concentration of PGF_2α was generally insignificant in contrast to PGD_2 orPGE_2 but still apparent in thalamus and hypothalamus with 4.2±0.8 and2.7±0.6 ng/g wet weight respectively.

덱사메사돈이 흰쥐의 뇌의 푸로스타글란딘 D2 함량에 미치는 영향

박시원,이상섭 ( Sie Won Park,Sang Sup Lee ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.3

The present study was performed to investigate the effect of dexamethasone on the basal level of PGD₂ of rat brain. Primarily, several steroid hormones were administered orally for one week (10 ㎎/ℓ drinking water) and dexamethasone thereof was shown to have the most strongly decreasing effect (40%) on the level of PGD₂ of the whole brain. When dexamethasone was administered orally to find out time dependency, it was demonstrated that there was a steadily increasing abatement of PGD₂ in the whole brain from 1st day onward to 7th day of uptake. In a similar way, there was also a fast reduction of PGD₂ content with maximum value of 35% by route of intraperitoneal injection. Furthermore, this result of PGD₂ decrease by injection showed typical circadian rhythm with peak value (2.4 ng of PGDZ/whole brain) at 5 : 00 PM. In an attempt to observe the dose dependency, this steroid was administered intraperitoneally in concentrations of 10 ㎍ to 1,000 ㎍/100g body weight. According to the injected dose, there was a gradual increment of decreasing effect of dexamethasone rather dose dependently to the concentration of 500 ㎍/100g body weight. As the effect of dexamethasone on the level of PGD₂ was very evident, we dissected the brain into 13 parts in order to find out the responsible area for specially reduced content of PGD₂ in the brain. However, the obtained data showed that dexamethasone exerts the decreasing effect on the basal level of PGD₂ generally with similar ratio (18∼29%) in almost all areas. This result implied that dexamethasone may be involved in the physiological homeostasis by feed-back control acting on adrenal-hypothalamus axis as known already and additionally may take part in some other central nervous functions via PGD₂ derived from cell membrane by being distributed overally.

노화과정에 따른 뇌신경의 핵단백질의 변화

박시원 생화학분자생물학회 1993 BMB Reports Vol.17 No.3

Rat cerebellum nuclei showed some significant changes of non-histone chromosomal protein (NHCP), a kind of chromosomal protein, as a function of age by electrophoresis profile analysis. Non-histone chromosomal protein was found to be prominent in peak numbers 23, 24 and 37 during developmental stages generally. In adult cerebellum, peak numbers 1, 4, 22 and 26 were distinct as compared with those of neonatal or fetal rats. Further, electrophoresis profile of high mobility group (HMG) protein included in the nonhistone chromosomal protein category revealed the presence of HMG bands 1, 2 and 17 in both neonatal and adult stages but HMG 14 band was distinct in adult cerebellum exceptionally. The HMG protein pattern obtained from nerve cell types demonstrated no much difference between the neuron and glia cell rich found fractions of adult cerebellum.

문화주도적 젠트리피케이션 지역의 도시관광자원과 만족도, 재방문의도, 추천의도 간 인과관계에 관한 연구 - 관광동기의 정도에 따른 차이 고찰 -

박시원 관광경영학회 2016 관광경영연구 Vol.69 No.-

This research is started with a necessity to maintain a continuance development of city tour and unaffected by gentrification's negative effect. So this research is focused on Itaewon-GyeongLiDan St. which is a cultural-led gentrification region. Because this region is a famous city tour place in Seoul. To accomplish a object of this research, both literature investigation and empirical analysis is used. For now, from a literature investigation, measurement item is deducted and survey is executed. From October 1 2015 to October 11 2015, Date were collected from 423 visitors of the Itaewon-GyeongLiDan St., which is a representative cultural-led gentrification region in Korea. Excepting a 92 insincerity & errored questionnaire, total 331 questionnaire is used to empirical analysis. Result of empirical analysis showed that the Cultural-Resource of Itaewon-GyeongLiDan St. is more effective than other factors. A Leisure-Resource and City-Resource is followed. And Overall Satisfaction is influence on Revisit and Recommend Intention. And a result of extra analysis showed that the effect relation of Overall satisfaction, Revisit and Recommend Intention of first visitors is lower than others.

휴양형 리조트 이용자들의 웰빙태도와 선택속성, 고객만족 및 행동의도 간의 인과관계에 관한 연구

박시원 ( Park Si-won ) 대한관광경영학회 2016 觀光硏究 Vol.31 No.8

본 연구는 휴양형 리조트를 방문한 고객들의 웰빙태도, 휴양형 리조트의 선택속성, 고객만족, 행동의도 간의 영향관계를 파악하고자 진행되었다. 이를 분석하기 위해서 선행문헌고찰을 통해 각 변수들 간의 관계를 가설 1~4로 설정하였다. 다음 휴양형 리조트 방문객들 중 휴양을 목적으로 휴양형 리조트를 방문한 고객들을 대상으로 설문조사를 수행하였다. 설문조사 결과, 총 332부의 표본이 수집되었으며 이를 토대로 실증분석을 수행하였다. 먼저 가설 1검증 결과, 리조트 방문객들의 웰빙태도는 휴양형 리조트 선택속성에 부분적으로 유의한 영향을 미치는 것으로 나타나 부분채택 되었다. 다음으로 가설 2를 검증한 결과 휴양형 리조트 선택속성은 선행연구들과 같이 고객만족에 유의한 영향을 미치는 것으로 나타나 가설 2를 채택하였다. 가설 3을 검증한 결과, 리조트 방문객들의 웰빙태도도 고객만족에 유의한 영향을 미치는 것으로 나타나 채택되었으며, 가설 4도 고객만족이 고객들의 재방문 의도와 추천 의도에 유의한 영향을 미치는 것으로 나타나 채택되었다. 이와 같은 연구결과에서 도출한 본 연구의 학술적 시사점으로는 환대산업에서는 주로 외식분야에서 활용되던 웰빙태도 개념을 리조트 연구분야에 도입하였다는 점이며, 실무적 시사점으로는 휴양리조트 방문고객들은 주로 정서안정을 위해 방문을 하므로 이와 관련된 마케팅전략 수립의 필요성을 도출하였다는 점에 본 연구의 의의가 있다. This study is conducted to figure out the Influence Relation among Wellbeing Attitude of the customers who visit the vacation resort, Selection Attributes for the vacation resort, Customer Satisfaction, and Behavioral Intention. So as to analyze that, this study conducts a survey aiming at the customers who visit the vacation resort for the purpose of relaxation, among the visitors to the vacation resort. The total number of the samples collected by this survey is 332, and the Actual Analysis is conducted on the basis of that result. The testing of hypothesis 1 suggests that Wellbeing Attitude of the customers who visit the vacation resort has a partially significant influence on Selection Attributes for the vacation resort, so hypothesis 1 is partially adopted. The testing of hypothesis 2 suggests that, like the preceding studies, Selection Attributes for the vacation resort has a significant influence on Customer Satisfaction, so hypothesis 2 is adopted. At last, the testing of hypothesis 3 suggests that Wellbeing Attitude of the customers who visit the vacation resort also has a significant influence on Customer Satisfaction. And the testing of hypothesis 4 suggests that Customer Satisfaction has a significant influence on Revisit Intention and Recommendation Intention. Based on the above results, both hypothesis 3 and 4 are adopted. The academic implication of this study is that the notion of Wellbeing Attitude which is utilized mostly in the field of Food Service as to Hospitality Industry is introduced in the field of Study on Resort. And then, the practical implication is that, since the customers visit the vacation resort primarily for the purpose of emotional stability, necessity for the establishment of a marketing strategy in reference to that result is deduced.

노화과정에 따른 뇌신경의 핵단백질의 변화

박시원 ( Sie Won Park ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.1

Rat cerebellum nuclei showed some significant changes of non-histone chromosomal protein (NHCP), a kind of chromosomal protein, as a function of age by electrophoresis profile analysis. Non-histone chromosomal protein was found to be prominent in peak numbers 23, 24 and 37 during developmental stages generally. In adult cerebellum, peak numbers 1, 4, 22 and 26 were distinct as compared with those of neonatal or fetal rats. Further, electrophoresis profile of high mobility group (HMG) protein included in the nonhistone chromosomal protein category revealed the presence of HMG bands 1, 2 and 17 in both neonatal and adult stages but HMG 14 band was distinct in adult cerebellum exceptionally. The HMG protein pattern obtained from nerve cell types demonstrated no much difference between the neuron and glia cell rich found fractions of adult cerebellum.

기후변화 대응을 위한 대안적 방법으로서의 미국 환경소송에 대한 고찰

박시원(Park, Si-Won) 강원대학교 비교법학연구소 2013 江原法學 Vol.38 No.-

온실가스를 규제하는 연방정부 차원의 법제와 정책수단이 전무한 가운데 미국 주정부와 시민단체는 환경소송 절차를 활용하여 기후변화 정책의 방향을 바꾸는데 주된 역할을 하였다. Massachusetts v. EPA 판결에 따라 온실가스는 청정대기법 하 대기오염물질이며 미 연방 환경청(Environmental Protection Agency, EPA)은 온실가스를 규제할 권한을 갖게 되었다. 4년 후 미 대법원은 American Electronic Power Co. v. Connecticut 판결에서 온실가스 규제의 유일한 근거법은 청정대기법 임을 다시 한번 확인하였다. 미국의 환경소송은 기후변화 피해에 대한 문제점 인식을 제고하고, 기후변화 과학을 발전시키는 역할을 하였다. 동시에 소송을 통한 규제 제고는 어느 정도 한계를 가지고 있는데 미국 사법부는 기후변화와 관련하여 스스로 심사 가능 여부를 제한하려는 경향을 보이고 있다. 온실가스 규제관련 정책과정에 행정부, 입법부, 사법부, 시민사회 등 모든 사회 구성원의 적극적인 참여가 필요하고 이 과정에서 기후변화 문제를 심각하게 받아들이는 사회적 공감대 형성이 필요하다. 궁극적으로 정부의 기후변화 정책, 그리고 더 넓은 의미에서 환경 정책 의사결정과정에 적극적인 시민 참여를 독려하기 위해서는 보다 활발한 환경 소송이 허용되어야 하고 이를 위한 현행법의 변화가 필요할 것이다. The US states governments and environmental advocate groups have used litigation as a strategic tool to nudge the federal government to take actions in the issue area of climate change. The U.S. supreme court decision in Massachusetts v. EPA clarified that greenhouse gases are air pollutants under the Clean Air Act and the US Environment Protection Agency has the authority to regulate greenhouse gas. Four years after the Massachusetts v. EPA, the U.S. supreme court heard the climate related case again and reconfirmed that the Clean Air Act is the legal basis of climate regulations. Climate related litigations in the United States played a significant role in raising public awareness on negative impact of climate change and developing climate science. However, the impact of climate related litigation on climate policy have a limited role because the US courts have showed the trend in limiting the scope of judicial review in climate relate cases. In the policy making process in the field of greenhouse gas regulation, the active participation of all branches of government and civil society is pivotal. Through the active participation, the consensus on the seriousness of climate change can be made. Ultimately, vigorous exercise of environmental litigation will serve an important tool to promote participation of civil society in policy decision process of climate change in Korea as well. Therefore, appropriate legal and institutional changes will be needed for promoting environmental litigations.

자연자원을 활용한 휴양형 리조트의 서비스 품질, 웰빙가치, 고객만족 및 행동의도의 구조적 관계에 관한 연구

박시원(Park, Si-Won) 한국관광레저학회 2016 관광레저연구 Vol.28 No.11

생태손해배상제도 도입을 위한 협의제도 고찰

박시원 ( Siwon Park ) 한국환경법학회 2022 環境法 硏究 Vol.44 No.1

최근 인류가 경험하는 자연 생태계의 파괴는 지금까지의 인간 구제 중심의 환경법에서 생태 보존을 위한 환경법으로의 전환을 요구하고 있다. 이러한 관점에서 개별적, 개인적 환경침해의 패러다임을 넘어 생태손해 자체의 배상 가능성을 인정하는 것은 이러한 전환의 첫 걸음이 될 수 있다. 현재 생태손해를 명시하고 손해배상 방법론을 규정한 별도의 법률은 존재하지 않지만, 2014년 제정된 환경오염피해구제법 제14조에서 자연환경 침해에 대한 원상회복 청구를 언급하고 있어 이에 대한 활용 가능성을 생각해 볼 수 있다. 제14조만을 활용하여 법원에서 생태손해배상을 인정하는 것을 기대하는 것은 무리가 있기 때문에 대안적 분쟁해결방식인 조정제도를 활용할 수 있는 방안을 고찰해 보았다. 즉, 별도의 생태손해배상에 관한 법률에 제정되기 전까지 과도기적 방안으로 환경오염피해구제법 제14조 해석을 기반으로 환경분쟁조정제도를 활용하여 생태손해구제방안 도입을 위한 교두보를 마련해볼 수 있을 것이다. 이를 위해서는 현행 환경분쟁 조정법은 다음과 같이 개정될 필요가 있다. 첫째, 생태손해 개념을 명문화할 필요가 있다. 생태손해의 도입을 위해 우선 환경분쟁 조정법 정의 조항에 기존 ‘환경피해’라는 개념과 별도로 ‘생태손해’라는 개념을 추가하는 것이다. 둘째, 생태손해의 구제는 환경오염피해구제법 제14조와 일치하는 방향으로 금전배상 이외에 원상회복을 우선하여 요청할 수 있다는 것을 환경분쟁 조정법 제41조(원상회복)에 명문화한다. 추후 원상회복 방법론와 생태손해 산정에 관한 전문성을 제고하기 위하여 환경분쟁조정위원회 하에 전문위원회를 설치하거나, 생태손해 산정 및 원상회복 대체 회복 등의 방법에 대한 가이드라인을 준비하는 것도 필요할 것이다. 셋째, 환경분쟁 조정법하에서 생태손해구제 조항의 활용도를 높이기 위하여 환경분쟁조정위원회가 직권으로 생태손해 배상청구를 할 수 있는 명문규정을 두는 것이 바람직하다. 넷째, 순수한 의미의 자연 그 자체로의 생태손해의 경우, 환경단체가 생태손해의 회복 청구대리권자가 되도록 법을 개정할 수 있다. 환경분쟁 조정법을 개정하여 생태손해 개념을 포섭하는 것은 여러 가지 의미가 있다. 별도의 생태손해 개별법이 제정되기 전까지 생태손해에 관한 과도기적 경험을 쌓을 수 있다. 또한 일부 환경피해에 집중하여 재정이라는 경직된 방식으로 업무가 한정되고 있는 환경분쟁조정위원회에 새로운 유형의 환경갈등을 해결하도록 분쟁해결방식의 가능성을 확장한다는 의미가 있다. 제한적이나마 현행법을 활용하여 생태손해배상제도를 도입함으로써 새로운 유형의 환경분쟁을 대화와 타협, 창의적인 중재의 방식으로 해결할 수 있는 역량을 우리사회가 키울 수 있는 계기가 될 수 있기 희망한다. The recent climate crisis and COVID-19 experience urge us to transform our human-centric to eco-centric environmental law system. To recognize the natural resources damage could be a stepping stone for such paradigm shift. We do not have a specific and separate law on natural resources damage however, we do have the Act on Liability for Environmental Damage and Relief. The article 14 of the Act mentions the natural resource damage and restoration obligations, therefore, it is worthwhile to analyze the effect of the provision. The sole provision of the article 14 of the Act is too simple to use in the natural resources damage litigation and have the court’s recognition, the paper seeks to address the possibility to use the alternative dispute resolution. Until the separate law on natural resources damage is enacted, the Environmental Dispute Mediation Commission could be a good venue to explore the natural resources damage as a transition plan. For this path, the Environmental Dispute Mediation Law should be amended as follows. First, the law needs to stipulate the definition of the natural resources damage. For the introduction of the natural resources damage, the Environmental Dispute Mediation Law needs to include the concept of natural resources damage in addition to its existing environmental damage. Second, the law needs to be amended to stipulate the restoration should be considered first and the said amendment should be done at the article 41 of the Environmental Dispute Mediation Act (Restoration to the Original State). Furthermore, the details of the restoration methods and approaches as well as the calculation of natural resources damage should be developed under the environmental dispute mediation commissions. Third, the Environmental Dispute Mediation Law should expand the Ex Officio Mediation cases under the Article 30 and allow the commission to start the mediation process as to the natural resources damages. Fourth, the environmental NGOs should be allowed to initiate the natural resources damage cases when there is no personal interest involved therefore no legal person interest. To amend the Environmental Dispute Mediation Law is meaningful for some reasons. It could encourage more and more natural resources damage cases to be discussed at the public forum during the interim period until we have a new law on natural resources damages specifically. It could also encourage to expand the scope of the environmental dispute mediation commission so that it could have an impact on new type of environmental disputes. Although natural resources damage is yet to be recognized in a full scale by the courthouse, it is still meaning to take a one step by using the environmental dispute mediation law.

맞춤형 웨어러블 기기 제작을 위한 개발된 인쇄 가능 실리콘 기반 마이크로 메쉬 프린팅 방법

박시원,윤준수,김상준,김민구 대한기계학회 2023 대한기계학회 춘추학술대회 Vol.2023 No.5