筋小胞體의 ATPase Kinetics에 關한 硏究

朴映淳,李承兩,朴泰水,庾基洙,李 弘,金允坤 圓光大學校 1982 論文集 Vol.16 No.2

본 연구에서는 토끼 골격근 소포체에서 분리한 근소포체의 ATPase활성에 대한 일반적 특성과 수종의 2가 양이온(??)의 영향에 관한 연구를 하였다. ATPase활성과 Ca uptake에 미치는 2가 양이온의 저해 효과와 저해기작은 Dixon plot, Lineweaver-Burk plot, Hanes-Woolf plot, Woolf-Augustinsson-Hofstee plot, Eadie-Scatchard plot, Arrhenius plot등을 이용하여 분석하였으며 그 결과는 다음과 같다. 1. 근소포체의 ATPase는 ??과 ??의 존재하에서 활성화 되어지며 ??의 농도가 4mM일 때 ??의 최적 농도는 0.1mM이었다. 2. 근소포체의 ATPase는 ??-ATPase와 (??)-ATPase로 구성되어 있고 (??) - ATPase의 활성은 ??-ATPase의 활성보다 높았다. 3. ATPase의 활성화 에너지는 약 19Kcal/mol이었다. 4. ATPase 활성에 미치는 2가 양이온은 12.5㎛과 400㎛의 범위 내에서 이온 농도가 증가됨에 따라서 활성도는 점점 감소되었다. 5. ATPase활성율 50% 저해시키는데 2가 양이온의 농도는 Ki값과 같아서 ??, ??, ?? Pd ??에 대한 각각의 농도는 10㎛, 30㎛, 130㎛ 및 350㎛이었다. 특히 ??은 저 농도에서 (12.5-100㎛)는 활성제로, 고농도에서 (200-400㎛)는 저해제로 작용하는 것 같다. 6. 2가 양이온은 ATPase의 Km에는 변화를 주지 않으나 Vmax의 저하를 일으키는 것으로 보아 비경쟁 저해제임이 판정되었다. 7. 2가 양이온은 가역적 저해제임이 판명되었다. 8. Ca 吸收能에 대한 CuCl₂의 阻害는 50~500㎛의 CuCl₂농도에서 현저히 나타났으며 이 현상은 Ca 吸收能과 ATPase 活性과 밀접한 관계를 보여주는 것이다. General properties of the ATPase and the effects of the divalent cations (??) on the total ATPase activity of the fragmented sarcoplasmic reticulum (FSR) isolated from rabbit skeletal muscle were investigated in the present resent research. The inhibitory effect of divalent cations on the FSR ATPase activity and its mechanism were analyzed by various plots such as Dixon plot, Lineweaver-Burk plot, Hanes-woolf plot, Eadie-Scatchard plot and Arrhenius plot. The results obtained were as follows: 1. The FSR ATPase is activated by ?? and ??; the optimum concentration of is 0.1 mM in the presence of 4 mM MgCl₂. 2. The FSR ATPase could be distinguished as ??-ATPase and (??)-ATPase. The activity of (??)-ATPase predomiant over that of ??-ATPase. 3. The apparent energies of activation for total ATPase was approximately 19Kcal/mole. 4. The inhibitory effects of the divalent cations on the enzyme activity increased as the concentrations of the divalent cations increased in the concentrations between 12.5 and 400㎛. 5. Concentration of 50% inhibition for each divalent cation was almost identical with the inhibition constant (ki). The Ki's were 10,30,130, and 350㎛ for ??, ??, ??, and ??, respectively. ?? seemed to be anactivator at lower concentrations and an inhibior at higher concentratios. 6. The divalent cations did not change the Km but decreased the Vmax of the FRS ATPase, suggesting that they are noncompetitive inhibitors on the FSR ATPase. 7. The divalent cations were also found to be revesible inhibitors on the FSR ATPase. 8. The inhibitory effect of CuCl₂on a uptake increased as the concentration CuCl₂increased in the range between 50㎛.

관동맥질환에서 저밀도 지단백 입자 크기의 분포

임효영,김인숙,승기배,강동헌,전두수,장기육,박준철,이수영,홍순조,최규보 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.8

농촌 노인의 식품 기호도 조사

박기순,박영숙,김 순 순천향대학교 기초과학연구소 1996 순천향자연과학연구 논문집 Vol.2 No.2

A study about food preference of the elderly in rural area was performed to 110 home-living subjects by questionaires and personal interviews. We found that they prefered the sweet and hot than the salty and sour. Among five food groups vegetables and fruits were the most favorite food groups to the elderly. followed by meats, fishes, eggs and legumes. Fats, oils and sugars were the least favorite one. Cooked rice with legumes was the most liked food among the grains and starches. most of the elderly liked soy paste and soybean curd among meats, fishes, eggs and legumes and yogurt among milk and milk products. They liked dried seaweed, watermelon, percimon, lettuce, peach, chinese cabbage and cucumber among vegetables and fruits and enjoyed vegetable oils including sesami oil among fats and oils.

일부 대학생의 건강 인식에 관련된 요인

박종,김기순 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

In order to investigate the associated factors of university students with self-recognition of health status, a cross-sectional study was conducted. Data including self-recognition of health status, life style related with health and present subjective symptoms were collected through questionnaire survey, during March and August 1992 toward 3,338 students of a university in Kwangju City. 1,929 cases with self-estimated healthy group were compared with 1,409 cases with self-estimated unhealthy group. The result obtained were as follows ; 1. Among the subjects, 57.8% reported that they were healthy, 57.3% of male stutents estimated that they were healthy and 58.9% of female students recognized that they were healthy. 2. Among male groups, meal regularity, taste, frequency of meat intake and vegetable preference were associated with self-recognition of health status and among female group, effort to health promotion, meal regularity, taste, alcohol drinking were associated with that. 3. Prevalence of cardiorespiratory, neuromuscular, gastrointestinal and general symptoms were associated with self-recognition of health status between both groups of sex. 4. Among both sex groups, past history of some diseases was associated with self-recognition of health status. 5. By multiple logistic regression analys, meal regularity, cardiorespiratory, neuromuscular, gastrointestinat, general symptoms, past history of diseases and body mass index were associated with self-estimated health status between both groups of sex, while a grade(school year) was associated with that only among male and effort for health promotion was associated with that only among female group. In conclusion, the life styles were related with health and the present subjective symptoms of the students were proved to be associated with self-recognition of health status among university students. So planning of health maintenance and promotion program must consider various related factors with health.

學生이 바라는 理想的 敎授像 : 文獻을 通해서 본

朴基順 東亞大學校 學生生活指導硏究所 1982 學生硏究 Vol.11 No.-

APSM의 예측능 평가에 관한 연구

박기학,윤순창 한국환경과학회 2003 한국환경과학회지 Vol.12 No.3

The Pasquill-Gifford stability category is a very important scheme of the Gaussian type dispersion model defined the complex turbulence state of the atmosphere by A grade(very unstable) to F grade(very stable). But there has been made a point out that this stability category might decrease the predictability of the model because it was each covers a broad range of stability conditions, and that they were very site specific. The APSM (Air Pollution Simulation Model) was composed of the turbulent parameters, i.e. friction velocity( u_*), convective velocity scale( w_* ) and Monin-Obukhov length scale( L ) for the purpose of the performance increasing on the case of the unstable atmospheric conditions. And the PDF (Probability Density Function) model was used to express the vertical dispersion characteristics and the profile method was used to calculate the turbulent characteristics. And the performance assessment was validated between APSM and EPA regulatory models(TEM, ISCST), tracer experiment results. There were very good performance results simulated by APSM than that of TEM, ISCST in the short distance (<1415 m) from the source, but increase the simulation error(%) to stand off the source in others. And there were differences in comparison with the lateral dispersion coefficient( σ_y) which was represent the horizontal dispersion characteristics of a air pollutant in the atmosphere. So the different calculation method of σ_y, which was extrapolated from a different tracer experiment data might decrease the simulation performance capability. In conclusion, the air pollution simulation model showed a good capability of predict the air pollution which was composed of the turbulent parameters compared with the results of TEM and ISCST for the unstable atmospheric conditions.

요 침전물의 형태학적 변화에 관한 검토

박정오,윤기은,김순랑 서울保健大學 1996 論文集 Vol.16 No.1

We used midstream urine sedments from S. N. U. hospital patients in hematuria were examined with urine chemical analyzer. To better understand the bleeding mechanism of dysmorphic erythrocytes which might cause the nephron model in vitro. We have exposed the pH and osmolarity of suspension were systematically changed with in a range of pH 1 to 14 and 48 to 1484 mOsm/L. It was found that, our preliminary studies had suggested that morphological change of dysmorphic erythrocytes might still be to make it fully useful by phase-contrast microscopy and give a immediate answer or within 120 minutes. Futhermore, we could not confirmed by our criteria dysmorphism of their urinary erythrocytes. Such positive findings, although resulting from a small study, compel us to recommend this inexpensive method in the initial approach to patients with hematuria. In our opinion, it can greatly help the clinician in differentiating glomerular and non-glomerular bleeding from other cause of hematuria and channeling such patients towards the most appropriate Investigations.

조직학습과 조직기억

박우순,정기찬 東亞大學校 2005 東亞論叢 Vol.42 No.-

Organizational learning and organizational memory are the primary components in knowledge management. In this paper, the major argument is to emphasize the role of organizational memory to make knowledge management successful. The contents of organizational memory are information, knowledge, and experiences held by organizational members obtained through organizational learning, and organizational memory need to be understood as an interrelated concepts for effective management of organizations.

가뭄기간의 저수지 운영방안에 관한 연구

박기범,이순탁 한국환경과학회 2004 한국환경과학회지 Vol.13 No.12

In this study the results of optimal water supply analysis by operating constraints of reservoirs during drought period are as follows. During drought period, water supply reliability is possible about 97~61% by CASE 1-CASE 5. Water supply reliability is possible about 97.3% in case of the Andong dam and 87.7% in case of the Imha dam by CASE 3. Also, under the constraints of CASE 4, water supply reliability is possible about 87.5% in case of the Andong dam and 73.3% in case of the Imha dam. The reason what low of available water supply ratio is decreased inflow of Imha dam. When compare standard deviation of average storage with standard deviation of storage, stable storage can be secured during successive drought period. And it also can minimize shortage of water during drought. therefore, it is impossible that reservoir supply sufficient water but change of operating condition is better than pervious on that followed by full reservoir level. It is need that the study for optimal water supply during drought period has to be continued.

공급량 배분기법을 이용한 갈수기 병렬저수지 해석

박기범,이순탁 한국환경과학회 2006 한국환경과학회지 Vol.15 No.3