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Transforming growth factor beta receptor II polymorphisms are associated with Kawasaki disease
Choi, Yu-Mi,Shim, Kye-Sik,Yoon, Kyung-Lim,Han, Mi-Young,Cha, Sung-Ho,Kim, Su-Kang,Jung, Joo-Ho The Korean Pediatric Society 2012 Clinical and Experimental Pediatrics (CEP) Vol.55 No.1
Purpose: Transforming growth factor beta receptor 2 ($TGFBR2$) is a tumor suppressor gene that plays a role in the differentiation of striated cells and remodeling of coronary arteries. Single nucleotide polymorphisms (SNPs) of this gene are associated with Marfan syndrome and sudden death in patients with coronary artery disease. Cardiovascular remodeling and T cell activation of $TGFBR2$ gene suggest that the $TGFBR2$ gene SNPs are related to the pathogenesis of Kawasaki disease (KD) and coronary artery lesion (CAL). Methods: The subjects were 105 patients with KD and 500 healthy adults as controls. Mean age of KD group was 32 months age and 26.6% of those had CAL. We selected $TGFBR2$ gene SNPs from serum and performed direct sequencing. Results: The sequences of the eleven SNPs in the $TGFBR2$ gene were compared between the KD group and controls. Three SNPs (rs1495592, rs6550004, rs795430) were associated with development of KD ($P$=0.019, $P$=0.026, $P$=0.016, respectively). One SNP (rs1495592) was associated with CAL in KD group ($P$=0.022). Conclusion: Eleven SNPs in $TGFBR2$ gene were identified at that time the genome wide association. But, with the change of the data base, only six SNPs remained associated with the $TGFBR2$ gene. One of the six SNPs (rs6550004) was associated with development of KD. One SNP associated with CAL (rs1495592) was disassociated from the $TGFBR2$ gene. The other five SNPs were not functionally identified, but these SNPs are notable because the data base is changing. Further studies involving larger group of patients with KD are needed.
DACUM 기법을 활용한 상급종합병원 간호사의 직무기술서 개발
이선미(Lee, Sun Mi),김연희(Kim, Yeon Hee),심유미(Shim, Yu Mi),최진선(Choi, Jin Sun),유미(Yu, Mi) 한국간호행정학회 2017 간호행정학회지 Vol.23 No.5
Purpose: This study was done to clarify nurses" duties, tasks and task elements and to develop a job description for clinical nurses on general and intensive care units in tertiary-level hospitals, and dramatically reflect changing medical trends in Korea. Methods: The job description was developed based on the Developing a Curriculum Method (DACUM). The questionnaire included frequency, importance, and difficulty of duties, tasks and task elements, measured on a 4-point scale. Results were analyzed using SPSS version 21.0. Data were collected from September 4 to 7, 2017, and analyzed using descriptive statistics and a scattered graph. Results: The job description consisted of 10 duties, 38 tasks, and 51 elements. Of the 10 duties, the highest duty in order of importance was ‘Direct nursing care’ followed by ‘Infection control’. The highest duties according to frequency and difficulty were ‘Document and notify’ and ‘Research and quality improvement’. ‘Safety management’ and ‘Infection control’ were considered as relatively simple duties, however, these two duties were still included as important jobs for clinical nurses. Conclusion: The job descriptions for clinical nurses developed from this study contain nursing ethics and safety as well as infection control, to faithfully reflect clinical nurses’ jobs.
Mi-Ra Cho,Yu-Jin Kwon,Shin-Hye Kim,Jinseub Hwang,Jimin Kim,Jangmi Yang,Jeonghoon Ahn,Jae-Yong Shim 대한가정의학회 2022 Korean Journal of Family Medicine Vol.43 No.5
Background: The International Classification of Primary Care-2 (ICPC-2) is a classification method designed for primary care. Although previous studies have found that ICPC-2 is a useful tool for demonstrating the relationship between patients’ expectations and health providers’ diagnoses, its utility of ICPC-2 has yet to be fully studied in Korea. This study aimed to evaluate the practicality of ICPC-2 in Korean primary care.Methods: The study was conducted at primary care clinics in Seoul and Gyeonggi areas from October to November 2015. Third-year family medicine residents examined and analyzed the medical records of patients who visited pri-mary care physicians using ICPC-2, and the results were compared with those obtained using the International Classification of Diseases-10 (ICD-10) (Korean version: Korean Standard Classification of Diseases-7).Results: A total of 26 primary care physicians from 23 primary care clinics participated in the study. Furthermore, 2,458 ICD-10 codes and 6,091 ICPC-2 codes were recorded from the data of 1,099 patients. The common disease codes were vasomotor and allergic rhinitis (J30), according to ICD-10, and acute upper respiratory infection (R74) in ICPC-2. Comparing disease status by body systems, the proportion of gastrointestinal disease with ICD-10 codes was significantly higher than that with ICPC-2 codes (P<0.001). Furthermore, patients with >4 diagnoses accounted for 36% of the ICD-10 classifications, whereas those with >4 diagnoses accounted for 4% of the ICPC-2 classifica-tions.Conclusion: Introducing ICPC as a complementary means for diagnosing common diseases could be a practical approach in Korean primary care.
Shim, Ye Ji,Choi, Byung Yoon,Park, Kyo Hoon,Lee, Hyunju,Jung, Young Mi,Kim, Yu Mi Hindawi 2018 MEDIATORS OF INFLAMMATION Vol.2018 No.-
<P><B>Objective</B></P><P> We aimed to determine whether elevated levels of various inflammatory and immune proteins in umbilical cord blood are associated with an increased risk of newborn hearing screening (NHS) test failure in preterm neonates. </P><P><B>Methods</B></P><P> This retrospective cohort study included 127 premature singleton infants who were born at ≤33.6 weeks. Umbilical cord plasma at birth was assayed for interleukin (IL)-6, complement C3a and C5a, matrix metalloproteinase (MMP)-9, macrophage colony-stimulating factor (M-CSF), and endostatin levels using ELISA kits. Neonatal blood C-reactive protein (CRP) levels were measured within 2 hours of birth. The primary outcome measure was a uni- or bilateral refer result on an NHS test. Univariate and multivariate analyses were applied. </P><P><B>Results</B></P><P> Fifteen (11.8%) infants failed the NHS test. In the univariate analyses, high IL-6 and low C3a levels in umbilical cord plasma, funisitis, and an elevated CRP level (>5 mg/L) in the immediate postnatal period were significantly associated with NHS test failure. However, the levels of umbilical cord plasma MMP-9, C5a, M-CSF, and endostatin were not significantly different between infants who passed and those who failed the NHS test. Multiple logistic regression analyses indicated that elevated umbilical cord plasma C3a levels were independently associated with a reduced risk of NHS test failure, whereas elevated levels of umbilical cord plasma IL-6 and high CRP levels in the immediate postnatal period were significantly associated with NHS test failure. </P><P><B>Conclusions</B></P><P> Our data demonstrated that in preterm neonates, a systemic fetal inflammatory response reflected by umbilical cord plasma IL-6 and immediate postnatal CRP levels may contribute to the risk for NHS test failure, whereas the changes in complement activation fragments initiated <I>in utero</I> may have protective effect of hearing screen failure.</P>
심미경,유연옥 한국정서학습장애아교육학회 2001 정서ㆍ행동장애연구 Vol.17 No.2
This study was to look at the approach of developmental psychopathology to psychological disorder in childhood, and analyse characteristics of clinical cases on the basis of developmental perspective, which was for emphasizing the necessity of developing developmental therapy-teaching programme for children with emotional and behavior difficulties. There were 90 children aged 12 or less. The characteristics of children were analysed according to the children's sex, age, siblings, family structure, factors related to pregnancy and children's developmental history. Disordered developmental factors were also analysed. The results were discussed in terms of the necessity of developmental therapy-developmental teaching approach to children's psychological disorders in educational settings as well as clinical.
( Mi-jung Jun ),( Ju Hyun Shim ),( Joo Ho Lee ),( Gi-won Song ),( Yangsoon Park ),( Eunsil Yu ),( Sung-gyu Lee ),( Jihyun An ),( Danbi Lee ),( Kang Mo Kim ),( Young-suk Lim ),( Han Chu Lee ),( Young-h 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: It has been found that methionine adenosyltransferase 1A (MAT1A) gene, encoding isoenzymes MAT I/III, is dysregulated in hepatocellular carcinoma (HCC), and reduced MAT1A expression correlates with worse HCC prognosis. The X protein of hepatitis B virus (HBV) inhibits apoptosis in HCC cells through enhancing the expression of MAT2A gene, encoding MAT II. MA1A/MAT2A switch has been severally demonstrated to be involved in hepatocarcinogenesis. We aimed to investigate prognostic implication of MAT I and MAT II protein expression in HBV-infected patients undergoing hepatic resection for HCC. Methods: In this study, we used a tissue microarray constructed from archival surgical specimens of 166 patients with HBV-related HCC who underwent curative hepatectomy at Asan Medical Center. The tumor tissue microarray was immunohistochemically stained with primary antibodies against MAT I and MAT II. We examined pre- and post-surgical clinical factors related to MAT 1 and MAT II, using logistic regression analysis, and predictive effect of the two proteins on post-surgical recurrence and survival, using Cox proportional hazards model. Results: Of the 166 patients, 74.1% were male with a mean age of 52.8 ± 8.7 years, 94% were Child-Pugh class A disease, and 55.4% had liver cirrhosis. In terms of histological factors, most patients had solitary tumor (93.4%) and tumors of 5cm or less (74.7%). Microvascular invasion and Edmondson grade III/IV tumors were observed in 30.7% and 66.9%, respectively of the patients. During a median follow-up of 39 months (range 5-81 months), 12 deaths and 63 recurrences had been found, where 52 recurrences occurred early within 2 years after resection. MAT I and MAT II were positively expressed in 83.7% and 87.3%, respectively of the 166 tumor tissues. MAT I expression was independently associated with male and tumors of 5 cm or less (adjusted P<0.05 for both). Expression of MAT II had a significant relationship with only serum AFP >200 ng/mL (adjusted P<0.05). Multivariate Cox regression analyses showed that MAT II expression was significantly correlated with shorter times to overall and early recurrences (hazard ratios 9.97 and 8.26, respectively; adjusted P<0.05 for both), as was not positive MAT I (hazard ratio 1.13; P=0.730). Immunopositivity for two proteins did not influence overall survival (P>0.05 for both). MAT I : MAT II activity ratio below 1.0 was observed in 12.7% of the patients, and not significantly associated with post-surgical recurrence and survival outcomes. Conclusions: Immunohistological expression of MAT II in tumor may be helpful in predicting and monitoring tumor recurrence, especially in the early phase after hepatic resection, in patients with HBV-related HCC.
Translational Attenuation and mRNA Stabilization as Mechanisms of erm(B) Induction by Erythromycin
Min, Yu-Hong,Kwon, Ae-Ran,Yoon, Eun-Jeong,Shim, Mi-Ja,Choi, Eung-Chil American Society for Microbiology 2008 Antimicrobial Agents and Chemotherapy Vol.52 No.5
<B>ABSTRACT</B><P>Translational attenuation has been proposed to be the mechanism by which the <I>erm</I>(B) gene is induced. Here, we report genetic and biochemical evidence, obtained by using erythromycin as the inducing antibiotic, that supports this hypothesis. We also show that erythromycin increases the level of the <I>erm</I>(B) transcript by stalling the ribosome on the leader mRNA and thereby facilitating the stabilization and processing of the mRNA. Erythromycin-induced mRNA stabilization and processing were observed with an ochre stop at codons 11 to 13 of the leader but not with an ochre stop at codon 10. This suggests that erythromycin does not stall the ribosome before codon 11 of the leader reaches the aminoacyl site. Secondary structure analyses of the <I>erm</I>(B) transcripts by in vitro and in vivo chemical probing techniques identified conformational changes in the transcripts that result from induction by erythromycin. These findings demonstrate that stalling of erythromycin-bound ribosomes at leader codon 11 causes the refolding of mRNA into a conformation in which the translational initiation site for the structural gene is unmasked and renders <I>erm</I>(B) translationally active.</P>