The complete mitochondrial genome of a threatened loach (Sinibotia reevesae) and its phylogeny

Yuan‑Chao Zou,Bi‑Wen Xie,Chuan‑Jie Qin,Yong‑Ming Wang,Deng‑Yue Yuan,Rui Li,Zheng‑Yong Wen 한국유전학회 2017 Genes & Genomics Vol.39 No.7

In present study, the complete mitochondrial genome of Sinibotia reevesae was first sequenced using the next-generation sequencing technology and annotated using bioinformatic tools. The circular mitochondrial genome was 16,572 bp in length, and contained 13 proteincoding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 displacement loop locus. It presents a typical gene organization and order for completely sequenced cypriniformes mitogenomes. The control region could be divided into three parts included the extended termination associated sequence domain, the central conserved domain and the conserved sequence block. Interestingly, two stemloop domains were found in control region and OL region, respectively. Furthermore, phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Maximum likelihood and Bayesian analysis) both highly supported the close relationship of S. reevesae and Sinibotia superciliaris, which was in line with the previous classifications based on morphological and molecular studies. These data provide useful information for a better understanding of the mitogenomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.

Comparison of the Angiogenic Ability between SHED and DPSC in a Mice Model with Critical Limb Ischemic

Yong Zhou,Kuang Gu,Fengying Sun,Shoumei Xuan,Duohong Zou,Jiacai He,Xuyan Tang 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.4

BACKGROUND: Regenerative medicine by using stem cells from dental pulp is promising for treating patients with critical limb ischemic (CLI). Here, we investigated the difference in the angiogenetic ability of stem cells from human exfoliated deciduous teeth (SHED) and human dental pulp stem cells (DPSC). METHODS: SHED and DPSC were harvested from dental pulp and analyzed in flow- cytometry for detecting the expression of surface markers. Levels of angiogenetic marker were examined by RT-PCR and Western-blot. Eighteen immunodeficient mice of critical limb ischemic model were divided into three groups: SHED, DPSC and saline, which was administered with SHED, DPSC or saline intramuscularly. Histological examination was performed to detect the regenerative results. RESULTS: A highly expression of CD146 was detected in SHED. Moreover, cells with negative expression of both CD146 and CD31inSHEDwere more in comparison with those inDPSC. Expression of angiogenesis factors includingCXCL12,CXCR4,Hif- 1a, CD31, VEGF and bFGFwere significant higher in SHED thanDPSC by the RT-PCR and Western-Blot results. SHED induced more CD31 expression and less fibrous tissue formation in the critical limb ischemic model as compare with DPSC and saline. CONCLUSION: Both SHED and DPSC possessed the ability of repairing CLI. With expressing more proangiogenesis factors, SHED may have the advantage of repairing CLI.

<i>De novo</i> genetic variation associated with retrotransposon activation, genomic rearrangements and trait variation in a recombinant inbred line population of <i>Brassica napus</i> derived from interspecific hybridization with <i>Brassica rapa</i>

Zou, Jun,Fu, Donghui,Gong, Huihui,Qian, Wei,Xia, Wei,Pires, J. Chris,Li, RuiYuan,Long, Yan,Mason, Annaliese S.,Yang, Tae‐,Jin,Lim, Yong P.,Park, Beom S.,Meng, Jinling Blackwell Publishing Ltd 2011 The Plant journal Vol.68 No.2

<P><B>Summary</B></P><P>Interspecific hybridization is a significant evolutionary force as well as a powerful method for crop breeding. Partial substitution of the AA subgenome in <I>Brassica napus</I> (A<SUP>n</SUP>A<SUP>n</SUP>C<SUP>n</SUP>C<SUP>n</SUP>) with the <I>Brassica rapa</I> (A<SUP>r</SUP>A<SUP>r</SUP>) genome by two rounds of interspecific hybridization resulted in a new introgressed type of <I>B.?napus</I> (A<SUP>r</SUP>A<SUP>r</SUP>C<SUP>n</SUP>C<SUP>n</SUP>). In this study, we construct a population of recombinant inbred lines of the new introgressed type of <I>B.?napus</I>. Microsatellite, intron‐based and retrotransposon markers were used to characterize this experimental population with genetic mapping, genetic map comparison and specific marker cloning analysis. Yield‐related traits were also recorded for identification of quantitative trait loci (QTLs). A remarkable range of novel genomic alterations was observed in the population, including simple sequence repeat (SSR) mutations, chromosomal rearrangements and retrotransposon activations. Most of these changes occurred immediately after interspecific hybridization, in the early stages of genome stabilization and derivation of experimental lines. These novel genomic alterations affected yield‐related traits in the introgressed <I>B.?napus</I> to an even greater extent than the alleles alone that were introgressed from the A<SUP>r</SUP> subgenome of <I>B.?rapa</I>, suggesting that genomic changes induced by interspecific hybridization are highly significant in both genome evolution and crop improvement.</P>

English Teaching Quality Evaluation Model Based on the Combined Dempster–Shafer (DS) Theory and Support Vector Machine (SVM)

Zou Xiaowei,Liu Jinming,Liu Yong,Li Hui,Yang Haidong 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.12

Beta-asarone Induces LoVo Colon Cancer Cell Apoptosis by Up-regulation of Caspases through a Mitochondrial Pathway in vitro and in vivo

Zou, Xi,Liu, Shen-Lin,Zhou, Jin-Yong,Wu, Jian,Ling, Bo-Fan,Wang, Rui-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Beta-asarone is one of the main bioactive constituents in traditional Chinese medicine Acorus calamu. Previous studies have shown that it has antifungal and anthelmintic activities. However, little is known about its anticancer effects. This study aimed to determine inhibitory effects on LoVo colon cancer cell proliferation and to clarify the underlying mechanisms in vitro and in vivo. Dose-response and time-course anti-proliferation effects were examined by MTT assay. Our results demonstrated that LoVo cell viability showed dose- and time-dependence on ${\beta}$-asarone. We further assessed anti-proliferation effects as ${\beta}$-asarone-induced apoptosis by annexin V-fluorescein isothiocyanate/propidium iodide assay usinga flow cytometer and observed characteristic nuclear fragmentation and chromatin condensation of apoptosis by microscopy. Moreover, we found the apoptosis to be induced through the mitochondrial/caspase pathway by decreasing mitochondrial membrane potential (MMP) and reducing the Bcl-2-to-Bax ratio, in addition to activating the caspase-9 and caspase-3 cascades. Additionally, the apoptosis could be inhibited by a pan-caspase inhibitor, carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone (Z-VAD-FMK). When nude mice bearing LoVo tumor xenografts were treated with ${\beta}$-asarone, tumor volumes were reduced and terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assays of excised tissue also demonstrated apoptotic changes. Taken together, these findings for the first time provide evidence that ${\beta}$-asarone can suppress the growth of colon cancer and the induced apoptosis is possibly mediated through mitochondria/caspase pathways.

Conjugated polymers based on C, Si and N-bridged dithiophene and thienopyrroledione units: synthesis, field-effect transistors and bulk heterojunction polymer solar cells

Zhang, Yong,Zou, Jingyu,Yip, Hin-Lap,Sun, Ying,Davies, Josh A.,Chen, Kung-Shih,Acton, Orb,Jen, Alex K.-Y. Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.11

<P>A series of low band-gap conjugated polymers (PDTC, PDTSi and PDTP) containing electron-rich C-, Si-, and N-bridged bithiophene and electron-deficient thienopyrroledione units were synthesized <I>via</I> Stille coupling polymerization. All these polymers possess a low-lying energy level for the highest occupied molecular orbital (HOMO) (as low as −5.44 eV). As a result, photovoltaic devices derived from these polymers show high open circuit voltage (<I>V</I><SUB>oc</SUB> as high as 0.91 V). These rigid polymers also possess respectable hole mobilities of 1.50 × 10<SUP>−3</SUP>, 6.0 × 10<SUP>−4</SUP>, and 3.9 × 10<SUP>−4</SUP> cm<SUP>2</SUP> V<SUP>−1</SUP> s<SUP>−1</SUP> for PDTC, PDTSi, and PDTP, respectively. The combined high <I>V</I><SUB>oc</SUB> and good hole mobility enable bulk hetero-junction photovoltaic cells to be fabricated with relatively high power conversion efficiency (PCE as high as 3.74% for the PDTC-based device).</P> <P>Graphic Abstract</P><P>Conjugated polymers of thienopyrroledione and bridged-dithiophene units were synthesized and show high open circuit voltage and good hole mobility. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0jm03927f'> </P>

Indacenodithiophene and Quinoxaline-Based Conjugated Polymers for Highly Efficient Polymer Solar Cells

Zhang, Yong,Zou, Jingyu,Yip, Hin-Lap,Chen, Kung-Shih,Zeigler, David F.,Sun, Ying,Jen, Alex K.-Y. American Chemical Society 2011 Chemistry of materials Vol.23 No.9

<P>Two new low-band-gap conjugated polymers based on the polymerization between indacenodithiophene and 2,3-diphenylquinoxaline or phenanthrenequinoxaline were synthesized. Due to the fused phenanthrenequinoxaline unit, the polymer (PIDT-phanQ) possesses better planarity than PIDT-diphQ, resulting in an improved hole mobility in organic field-effect transistors and power conversion efficiency in polymer solar cells.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/cmatex/2011/cmatex.2011.23.issue-9/cm200316s/production/images/medium/cm-2011-00316s_0006.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/cm200316s'>ACS Electronic Supporting Info</A></P>

Synthesis, Characterization, Charge Transport, and Photovoltaic Properties of Dithienobenzoquinoxaline- and Dithienobenzopyridopyrazine-Based Conjugated Polymers

Zhang, Yong,Zou, Jingyu,Yip, Hin-Lap,Chen, Kung-Shih,Davies, Joshua A.,Sun, Ying,Jen, Alex K.-Y. American Chemical Society 2011 Macromolecules Vol.44 No.12

<P>Two donor–acceptor polymers (<B>P1</B> and <B>P2</B>) based on dithienobenzoquinoxaline (<B>M1</B>) and dithienobenzopyridopyrazine (<B>M2</B>) as acceptor and indacenodithiophene as donor were synthesized via Stille polycondensation. The fused dithienobenzene unit in <B>M1</B> and <B>M2</B> units can improve the intermolecular stacking of polymer and also decrease the steric hindrance. <B>P1</B>, with dithienobenzoquinoxaline acceptor, shows a band gap of 1.61 eV. The band gap of <B>P2</B> was reduced to 1.48 eV after changing to dithienobenzopyridopyrazine as the acceptor unit. The mobilities of <B>P1</B> and <B>P2</B> reach 5.6 × 10<SUP>–2</SUP> and 1.5 × 10<SUP>–2</SUP> cm<SUP>2</SUP> V<SUP>–1</SUP> s<SUP>–1</SUP>, respectively. The results from photovoltaic measurements showed a very promising PCE of 6.06% for the <B>P1</B>/PC<SUB>71</SUB>BM blend system without any thermal or solvent treatments, showing a great offer for the roll-to-roll manufacturing of PSCs.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/mamobx/2011/mamobx.2011.44.issue-12/ma2008699/production/images/medium/ma-2011-008699_0004.gif'></P>

Fabrication of Porous Silicon Nanotips by Using Argon Ion-beam Irradiation

Chang Yong Zhan,Yu Zou,Jian-Chun Wu,P. K. Chu 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.2

Porous silicon nanotips (NTs) are promising in many applications such as field emission, superhydrophobicity,solar cells and photoluminescence. Ion irradiation can create unique nanostructures in many types of materials at room temperature, and this paper reports the surface morphology of porous silicon irradiated by an Ar+ ion beam. Different porous silicons are irradiated, and the effects of the ion’s angle of incidence on the porous silicon nanostructure are investigated. Highdensity NTs of less than 50 nm in size are observed on the porous silicon. The NT size increases,but NT density decreases, with increasing pore size. The orientation of the NTs can be controlled by adjusting the ion impact. Our results reveal that the NTs located at the thick wall between pores and charges created on the tip during Ar+ ion irradiation are beneficial to the formation of the NTs.

Applications of Brillouin Dynamic Grating to Distributed Fiber Sensors

Kwang Yong Song,Hotate, Kazuo,Weiwen Zou,Zuyuan He IEEE 2017 Journal of Lightwave Technology Vol.35 No.16

<P>In the process of stimulated Brillouin scattering (SBS), acoustic waves are generated by the interference of counterpropagating optical waves via electrostriction effect. These acoustic waves not only stimulate the Brillouin scattering process, but can also play a role of moving Bragg reflector for another optical wave when a proper phase-matching condition is satisfied between the interacting optical waves. Brillouin dynamic grating (BDG) represents this secondary role of the acoustic wave, which shows a unique reflection spectrum, called BDG spectrum. The BDG spectrum generally shows higher sensitivity to the change of physical variables, such as temperature and strain than ordinary Brillouin gain spectrum (BGS), and also provides information on the waveguide parameters, such as polarization or modal birefringence with high accuracy. This paper reviews the operation principle of BDG under various conditions and the research progresses on its application to distributed fiber sensors.</P>