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Jin Tae Hong, Dohee Won1, Mi Hee Park, Sun Mi Kown, Miran Jo, Sang-Yoon Nam, Beom Jun Lee, Young Won Yun, Ki-Wan Oh, Sang Bae Han 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.4
The objective of this study was to determine the effect of macrophages on growth of human colon cancer cells. The results showed that co-culture of colon cancer cells with macrophages inhibited the growth of colon cancer cells (HCT116 and SW620) depending on the number of macrophages, RAW 264.7 cells, and activated THP-1 cells accompanied by down regulation of pSTAT3 in cancer cells. We also found that expression and release of cancer cell growth inhibitory cytokines, IL-1 receptor antagonist (IL-1ra) and IL-10, was increased in macrophages. Blocking of the STAT3 pathway with specific inhibitor and siRNA of STAT3 abolished the growth of colon cancer cells and expression of IL-1ra and IL-10. In addition, neutralization of IL-1ra and IL-10 with antibodies resulted in reversal of macrophage-induced inhibition of cancer cell growth. These data showed that IL-1ra and IL-10 released from macrophages inhibit growth of colon cancer cells through inhibition of the STAT3 pathway
Oh, Chung Ryul,Kim, Jeong Eun,Kang, Jihoon,Kim, Sun Young,Kim, Kyu-pyo,Hong, Yong Sang,Lim, Seok-Byung,Yu, Chang Sik,Kim, Jin Cheon,Kim, Jihun,Jang, Se Jin,Kim, Tae Won Elsevier 2018 Clinical colorectal cancer Vol.17 No.4
<P><B>Abstract</B></P> <P><B>Background</B></P> <P>We investigated whether the microsatellite instability (MSI) status affects the survival outcomes in patients with stage II/III rectal cancer who have undergone an upfront curative resection.</P> <P><B>Patients and Methods</B></P> <P>A total of 1103 patients with curatively resected stage II/III rectal cancer who had available polymerase chain reaction-based MSI results were included in the final analysis.</P> <P><B>Results</B></P> <P>Twenty-four (2.2%) patients in the total cohort were found to be MSI-high (MSI-H). In univariate analysis, neither disease-free survival (DFS) nor overall survival (OS) demonstrated significant differences between patients with MSI-H tumors and those with MSI-low (MSI-L) or microsatellite stable (MSS) tumors. The 5-year DFS rate was 78.0% in MSI-H patients and 69.9% in MSI-L/MSS patients (hazard ratio [HR], 0.84; 95% confidence interval [CI], 0.35-2.02; <I>P</I> = .689). The 5-year OS rates for MSI-H and MSI-L/MSS patients were 84.0% and 83.1%, respectively (HR, 0.86; 95% CI, 0.27-2.69; <I>P</I> = .790). By multivariate analysis, the MSI status did not affect either the DFS (HR, 1.00; 95% CI, 0.40-2.47; <I>P</I> = .994) or OS (HR, 0.85; 95% CI, 0.26-2.73; <I>P</I> = .778).</P> <P><B>Conclusions</B></P> <P>MSI-H tumors are rarely observed in rectal adenocarcinoma, and the MSI status may not affect the survival outcome in patients with a resected rectal cancer.</P>
Efficacy and safety of topical application of epidermal growth factor (EGF) for Korean acne patient
( Won Jong Oh ),( Eun Jung Ko ),( Sun Young Choi ),( Kwang Ho Yoo ),( Beom Joon Kim ),( Chang Kwun Hong ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2
Background: Commonly prescribed medications for acne vulgaris are oral antibiotics and retinoids. However, those have clinical limitation due to its resistance and teratogenicity, respectively. In that EGF helps reducing sebum secretion and enhances skin moisturizing, topicalapplication of EGF is implied as a new treatment modality for acne vulgaris. Objectives: We aim to evaluate the clinical efficacy and safety of topical recombinant human (rh) EGF in treating facial acne vulgaris. Methods: Twenty three Korean with mild acne vulgaris has applied topical rhEGF cream (Trouble control EGF) on the face twice daily for four weeks. Acne lesion counts were scored by investigator’s global assessment, while efficacy and satisfaction were evaluated by subjects. Skin sebum output, hydration and redness level were also measured at each visit. Results: Skin sebum level, transepidermal water loss, skin redness statistically decreased and acne lesions were significantly reduced. No severe side effects were observed during the study. Conclusion: Topical rhEGF seems to be an effective and safe adjuvant treatment option for mild acne vulgaris.
Sun-Young Kim,Sang-Won Jeon,Mi Yeon Lee,Dong-Won Shin,Weon-Jeong Lim,Young-Chul Shin,Kang-Seob Oh 대한신경정신의학회 2020 PSYCHIATRY INVESTIGATION Vol.17 No.1
Objective: The aim of this study is to determine the dose-response relationship between physical activity and anxiety symptoms. Methods: We included data of 124,434 participants who had comprehensive health-screening examinations from January 1st, 2012, to December 31st, 2016, in Kangbuk Samsung Hospital, Seoul and Suwon, South Korea. We measured the level of physical activity using the International Physical Activity Questionnaire-short form (IPAQ-SF) and estimated anxiety symptoms using the Beck Anxiety Inventory (BAI). BAI scores of 19 and above were defined as cases. Logistic regression was used to analyze the association between physical activity and BAI-defined anxiety. Furthermore, we assessed whether sex differences might affect the relationship between physical activity and BAI-defined anxiety by stratifying our data. Results: Compared with the sedentary group (0-600 METs-min/week), individuals achieving 600-6,000 METs-min/wk had a significantly lower risk of BAI-defined anxiety with a U-shaped relationship in general adults. After stratifying our data by sex, we found that optimal ranges of physical activity were 600-9,000 METs-min/wk for men, but 1,200-3,000 METs-min/wk for women. Conclusion: We identified a U- or J-shaped association between physical activity and anxiety symptoms, suggesting an optimal dose and upper limit of physical activity for decreasing anxiety symptoms. Optimal levels and upper limits of physical activity for reducing anxiety symptoms were higher for men than for women.
Efficient Cryopreservative Conditions for Cultivated Limbal and Conjunctival Epithelial Cells
Oh, Joo Youn,Kim, Mee Kum,Shin, Kyung Seon,Shin, Mi Sun,Wee, Won Ryang,Lee, Jin Hak,Ko, Seon Mi,Lee, Jae Lim Wolters Kluwer Health, Inc. All rights reserved. 2007 Cornea Vol.26 No.7
PURPOSE:: To examine the effects of cryopreservation on the viability of cultivated corneal limbal and conjunctival epithelial cells and to evaluate the optimal conditions for cryopreservation. METHODS:: The cultivated human limbal epithelial cells (HLECs) were stored in media including 20%, 50%, and 90% fetal bovine serum (FBS) and 10% dimethyl sulfoxide (DMSO) at −196°C for 1 week. The cultivated rabbit conjunctival epithelial cells were stored in 10%, 20%, and 50% FBS with 10% glycerol or DMSO as a cryoprotectant at −196°C for 1 week. After thawing, cell viability was assessed using the trypan blue vital staining and 3-[4,5-dimethylthiazol-2-yl]-2,5-dephenyl tetrazolium bromide (MTT) assay. Immunofluorescent staining was performed with cytokeratin 3/12 antibody. Colony-forming efficiency (CFE) was evaluated 2 weeks after culture. RESULTS:: HLECs cryopreserved with 50% FBS showed the highest cell viability, whereas those with 20% FBS revealed the lowest survival rate (87.1% ± 0.8% and 79.8% ± 4.01%, respectively; P = 0.030). CFE of HLECs was 2.13 ± 1.35%, 2.31 ± 2.23%, and 1.94 ± 0.72% in cells with 20%, 50%, and 90% FBS, respectively (P > 0.05). For conjunctival epithelial cells, the cell viability was the highest with 50% FBS and 10% glycerol (95.0% ± 4.27%), and the lowest survival rate was observed in the condition of 10% FBS and 10% DMSO (80.0% ± 5.49%). CFE of cryopreserved conjunctival epithelial cells was 14.1% ± 1.9% in cells with 20% FBS and glycerol and 13.5% ± 2.0% in those with 20% FBS and DMSO (P > 0.05). HLECs expressed CK3/12 after cryopreservation in all conditions examined. CONCLUSIONS:: The best results were yielded by 50% FBS for cell viability in HLECs. Glycerol seems to be superior to DMSO in cell viability of the rabbit conjunctival epithelium after cryopreservation.
Oh, Sun Kyung,Kim, Hee Sun,Ahn, Hee Jin,Seol, Hye Won,Kim, Yoon Young,Park, Yong Bin,Yoon, Chul Jong,Kim, Dong-Wook,Kim, Seok Hyun,Moon, Shin Yong Wiley (John WileySons) 2005 Stem cells Vol.23 No.2
<P>Here we report the derivation and characterization of new human embryonic stem cell (hESC) lines, SNUhES1, SNUhES2, and SNUhES3. These cells, established from the inner cell mass using an STO feeder layer, satisfy the criteria that characterize pluripotent hESCs: The cell lines express high levels of alkaline phosphatase, cell surface markers (such as SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), transcription factor Oct-4, and telomerase. When grafted into severe combined immunodeficient mice after prolonged proliferation, these cells maintained the developmental potentials to form derivatives of all three embryonic germ layers. The cell lines have normal karyotypes and distinct identities, revealed from DNA fingerprinting. Interestingly, analysis by electron microscopy clearly shows the morphological difference between undifferentiated and differentiated hESCs. Undifferentiated hESCs have a high ratio of nucleus to cytoplasm, prominent nucleoli, indistinct cell membranes, free ribosomes, and small mitochondria with a few crista, whereas differentiated cells retain irregular nuclear morphology, desmosomes, extensive cytoplasmic membranes, tonofilaments, and highly developed cellular organelles such as Golgi complex with secretory vesicles, endoplasmic reticulum studded with ribosomes, and large mitochondria. Existence of desmosomes and tonofilaments indicates that these cells differentiated into epithelial cells. When in vitro differentiation potentials of these cell lines into cardiomyocytes were examined, SNUhES3 was found to differentiate into cardiomyocytes most effectively.</P>