주의력결핍과잉행동장애와 Alpha-1C-Adrenergic 수용체 유전자와의 연관성에 대한 연구

조수철,김재원,김붕년,황준원,박미라,김순애,조대연,유희정,정운선,손정우,박태원 大韓神經精神醫學會 2008 신경정신의학 Vol.47 No.1

Objectives : Neurobiological and pharmacological research has suggested that the dysregulation of the central noradrenergic systems might be involved in the pathophysiology of ADHD. The aim of this study was to examine the association of the alpha-1C-adrenergic receptor gene (ADRA1C) with ADHD in Korean subjects. Methods : In a case-control study, we assessed 186 DSM-IV ADHD probands and 150 normal controls. One hundred and eight trios were studied in a family-based association analysis. Psychiatric diagnoses were derived through structured diagnostic interviews. For the clinical evaluation of the ADHD subjects, the Child Behavior Checklist (CBCL), the ADHD Rating Scale-IV (ARS) and the Junior Temperament and Character Inventory (JTCI) were administered. A computerized continuous performance test (CPT) was used to measure the inattention and impulsivity of the ADHD children. Results : There were no significant differences in the genotype or allele frequencies of the ADRA1C PstI polymorphism between the ADHD and control group (p>0.05). The transmission disequilibrium test (TDT) analysis observed no evidence for biased transmission of any of the alleles of the PstI polymorphism. There were no significant differences in the CPT or JTCI profiles between those ADHD subjects with the CC genotype and those with the other (CT+TT) genotypes at the PstI polymorphism. Conclusion : The results of this study do not support the ADRA1C as a major genetic susceptibility factor in ADHD.

미세 패턴에서 공정 여유도를 고려한 선폭 분석

정연운,조선영,오진경,오혜근 漢陽大學校 工學技術硏究所 1997 工學技術論文集 Vol.6 No.1

The linewidth variation with different process variables, such as exposure dose, thickness of PR, thickness of thin film, and the optical property of thin film, was studied for deep UV light and resist developed 0.25 μm isolated line pattern. We found that the PR thickness which gave minimum linewidth showed larger exposure latitude and smaller linewidth variation compared to the PR thickness which gave maximum linewidth. Thus it will provide better process latitude and smaller linewidth variation if we use this PR thickness.

야간 환자 발생양상에 관한 조사연구

원광연,심운택,조영채 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

This study was conducted with 800 patients, who came to the emergency rooms of hospital and clinic at night time of Cheongju medical bloc, to look for the characteristics of the occurance of: emergency patients at night time, for randomly sampled 7 days containing each different day from April 1st. to April 30th. 1987 on the basis of such various factors as age, sex, residence and status of patients. The following conclusions were obtained 1. The percentage of emergency patients by sex were 60.1% in male and 33.9% in female, making the ratio 1.54 : 1. The age group of 10s and 20s occupied 40.3% of all patients. 2. The percentage of each disease was 41.1% in injury and poisoning, 17.2% in disease of the respiratory system, 16.1% in disease of the digestive system. 3. Male had a higher incidence in the injury and poisoning, disease of the respiratory system, infectious and parasitic disease, but disease of the digestive system, disease of the nervous system and sense organ, complication of pregmancy, child birth and the p lerperium were higher in female. 4. Age distribution of emergency patients showed as followes : Injury and )oisoning had a higher percentage in the age group of 10-49 years, disease of respiratory system under 1 years of age, disease of the digestive system over 30 years of age, disease of circulartory system in 40-50 years of age. 5. Disease distribution on residential area revealed that residents out of city had higher propor tion of injury and poisoning, infectious and parasitic disease than the ones in city. The time of staying in the emergency room were less than 1 hour in 69% of all patients. 6. The status of patients when leaving the emergency room were complete cure(68.7%), admission(17.9%) self-discharge (12.5%), and death(0.9%).

Mapping of qBK1, a major QTL for bakanae disease resistance in rice

Hur, Yeon-Jae,Lee, Sais Beul,Kim, Tae Heon,Kwon, Tackmin,Lee, Jong-Hee,Shin, Dong-Jin,Park, Soo-Kwon,Hwang, Un-Ha,Cho, Jun Hyeon,Yoon, Young-Nam,Yeo, Un-Sang,Song, You-Chun,Kwak, Do-Yeon,Nam, Min-Hee Springer-Verlag 2015 Molecular breeding Vol.35 No.2

재실자 착의량 산출을 위한 CNN 기반 의복 분류 모델 성능 분석

조혜운(Hye Un Cho),최은지(Eun Ji Choi),조지현(Ji Hyeon Cho),현지연(Ji Yeon Hyun),김남현(Nam Hyeon Kim),문진우(Jin Woo Moon) 대한건축학회 2021 대한건축학회 학술발표대회 논문집 Vol.41 No.1

Fine mapping of the Grh3, Conferring Resistance to Green Rice Leafhopper (Nephotettix cincticeps Uhler) in Rice, Oryza sativa L.

Jong-Hee Lee,Yeon-Jae Hur,Do-Yeon Kwak,Jun-Hyun Cho,Yeong-Nam Yoon,Bong-Choon Lee,Ji-Yun Lee,Sang-Yeong Kim,Yeong-Bo Sohn,Un-Sang Yeo,Yu-Chun Song,Choon-Woo Lee,Min-Hee Nam,Jae-Keun Sohn 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The green rice leafhopper (GRH), Nephotettix cincticeps Uhler, is one of the most serious insect pests affecting cultivated rice (Oryza sativa L.) in temperate regions of East Asia. To understand the genetic basis of the GRH resistance, a F2 population derived from across between a highly resistant variety,Cheongnam and a susceptible variety, Junambyeo was analyzed by genetic analysis and association mapping. GRH resistance was evaluated using the F2 populations. The results showed that a single dominant gene in Cheongnam. DNA from 22 F2 individuals being either resistant or susceptible were pooled to produce bulk resistant and bulk susceptible DNA samples. Parents and bulks were screened with 192 SSR markers and twolinked SSRmarker, RM6082 and RM20145 were identified.Subsequent mapping in the original mapping population showed that thelocusis flanked by the SSR markers, RM20130 and RM20152 on chromosome 6. To physically map this locus, the-linked markers were landed on the artificial chromosome clones of the reference cv., Nipponbare, released by the International Rice Genome Sequencing Project. The DNA markers found to be closely linked to Grh3 would be useful for marker-assisted selection for the improvement of resistance to GRH in rice.

Identification of a major quantitative trait locus for bakanae disease resistance in rice

Yeon-Jae Hur,Saes-Beul Lee,Tae-Heon Kim,Jong-Hee Lee,Dong-Jin Shin,Soo-Kwon Park,Woon-Ha Hwang,Sang-Ik Han,Jun-Hyun Cho,Young-Nam Yoon,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Bakanae disease incidence threat is an increasing trend in the top rice growing countries. Despite it is essential to identify the resistant genes and underlying mechanisms of bakanae disease to develop resistant varieties, there are very limited genetic studies on bakanae disease in rice. The indica rice variety Shingwang was selected as resistant donor to bakanae disease. One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). The proportion of healthy plants of Shingwang and Ilpum after inoculation was confirmed using bakanae disease pathogen, CF283. While inoculated Ilpum showed thin and yellowish-green phenotype which is typical symptom of Bakanae disease, Shingwang showed similar healthy phenotype with control plants. A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The information of qBK1 could be useful for improving rice bakanae disease resistance in marker-assisted breeding.

PCR-based allele specific markers for bacterial blight resistance gene in rice.

Yeon-Jae Hur,Jong-Hee Lee,Ji-Ung Jeung,Ji-Yoon Lee,Jun-Hyun Cho,Hyun-Jin Park,You-Chun Song,Myung-Gyu Oh,Un-Sang Yeo,Choon-Woo Lee,Min-Hee Nam 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The use of functional markers, it is expected to make direct identification about genetic diversity at DNA level and overcome the problem of recombination /linkage. These markers can be used to identify interesting alleles in a breeding program and indirectly select for the trait, saving money, time and labor. Bacterial blight of rice caused by Xanthomonas oryzaepv. Oryzae is a destructive disease in rice production worldwide. No bactericide is effective to control the bacterial blight disease yet. Xa3, which is a gene conferring resistance to BB of the rice plant has been previously characterized by map-based cloning. We have cloned and sequenced the Xa3/xa3 gene in Korean cultivar, Hwayoung, Ilmi and Goun with gene specific primers. Our work detected polymorphisms and PCR-based allele specific SNP markers were developed. Susceptible or resistant individuals from an F2 population developed from across between Milyang244 and Ilmi, Korean germplasms and near isogenic lines carrying BB resistance genes were screened with allele specific markers. We found that the genotype completely matched their phenotype to BB using ASP-primers. These markers could be effective to marker-assisted selection for the Xa3 gene in rice breeding programs.

Real-time PCR을 이용한 가축생균제용 유산균 정량분석

Yeon Jae Choi(최연재),Sun Ho Kim(김선호),Min Jeong Gu(구민정),Han Na Choe(최한나),Dong Un Kim(김동운),Sang Bum Cho(조상범),Su Ki Kim(김수기),Che Ok Jeon(전체옥),Gui Seok Bae(배귀석),Sang Seok Lee(이상석) 한국생명과학회 2010 생명과학회지 Vol.20 No.12

본 연구는 가축생균제용 유산균을 Real-time PCR정량분석법을 이용하여 분석하였다. SYBR Green1 방법과 Probe 방법을 이용하여 표준곡선을 제작한 결과, SYBR Green1 방법에서는 Slope 값이 -3.346이었고, Y절편은 33.18, R² 값은 0.993으로 나타났으며, Probe 방법에서는 Slope값이 -3.321이었고, Y절편은 39.10, R² 값은 0.995로 나타나, 이를 이용한 표준곡선 제작이 가능함을 알 수 있었다. SYBR Green1 방법을 이용한 생균제의 Lactobacilli 정성ㆍ정량 분석결과 Real-time PCR값은 4.46~6.56 log copies로 나타났고, 생균수 측정 결과 값은 5.63~7.59 log CFU/g로 나타났으며, Probe 방법을 이용한 생균제의 Lactobacilli 정성ㆍ정량 분석결과에서는 Real-time PCR 값은 5.51~7.00 log copies로 나타났으며, 생균수 측정 결과 값은 5.63~7.59 log CFU/g로 나타났다. 본 연구에서 실시한 RT PCR법은 3~4일이 소요되는 기존의 배지법과 비교하여 24시간 이내에 신속하게 검출이 가능하다고 여겨지며, 또한 RT PCR을 이용한 분석방법에서도 dye 사용과 primer 사용에 따라 결과값이 차이가 나타났음을 확인할 수 있었으며, Probe 방법을 이용하여 실험 한 결과가 민감한 결과를 나타내었음을 확인 할 수 있었다. This study was conducted using quantitative real-time PCR using Lactobacilli as probiotics. Quantitative real-time PCR (RT PCR) was conducted via a method involving SYBR Green 1 and a probe. Plasmid DNA was cloned using the 16S-23S rRNA intergenic species region. Gene clones were diluted from 10² to 10¹?. Standard curves were constructed via Ct values obtained from the results of Real-time PCR via the aforementioned SYBR Green 1 and probe method. Plasmid DNA was also cloned using the 16S-23S rRNA intergenic species region and the gene clones were diluted from 10² to 10¹? copy numbers via the probe method. Using RT PCR, a standard curve of plasmid DNA copy numbers was also determined. The slope value for the Y-axis intercept and R² value were measured as -3.346, 33.18, and 0.993, respectively, via the first method. For the second method, the slope value for the Y-axis intercept and R² were -3.321, 31.10 and 0.995, respectively. The PCR inhibitor could not express the detection curve at a copy number over 10¹? via either method, owing to high DNA density. The DNA extract from probiotics was diluted without pre-culturing, and 16 products were amplified via both methods. The Ct value was 11.06~18.12 in the first method and 16.74~22.11 in the second method. Measured probiotics and log copy values were largely similar among the methods used. It was concluded that both methods are effective for analysis, but further research will be required to verify the optimal method.

QTL analysis of the qBK1, a major QTL for bakanae disease resistance in rice

Yeon-Jae Hur,Saes-Beul Lee,Tae-Heon Kim,Jong-Hee Lee,Dong-Jin Shin,Soo-Kwon Park,Woon-Ha Hwang,Sang-Ik Han,Jun-Hyun Cho,Young-Nam Yoon,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Bakanae disease is one of the most serious and oldest problems of rice production, which was first described in 1828 in Japan (Ito and Kimura 1931). This disease may infect rice plants from the pre-emergence stage to the mature stage, with severe infection of rice seeds resulting poor germination or withering (Iqbal et al. 2011). Under favorable environmental conditions, infected plants have the capacity to produce numerous conidia that subsequently infect proximate healthy plants, resulting in major yield loss (Ou 1985). One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The results of this study are expected to provide useful information toward developing resistant rice lines to this detrimental fungal disease.