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허재연,정창호,심기환 경상대학교 농업생명과학연구원 2003 농업생명과학연구 Vol.37 No.1
기능성 식품재료로서 환삼덩굴의 줄기와 잎의 이용성을 향상시킬 목적으로 암, 수로 구분하여 화학성분을 분석하였다. 일반성분 중 가용성 무질소물과 조단백질 함량은 잎에서 높게 나타났으며, 수분과 조섬유는 줄기에서 높게 나타났고, 줄기와 잎의 주요 무기성분은 K, Ca, P 및 Mg으로 나타났다. 유리당을 분석한 결과 숫잎과 암잎에서 fructose는 각각 0.16%와 0.10%로 나타났으며, 숫줄기와 암줄기에서 sucrose는 각각 0.37%와 0.24%로 나타났다. 비휘발성 유기산을 분석한 결과 acetic acid가 잎(4.99 mg%와 3.07 mg%)과 줄기 (3.18 mg%와 2.25 mg%)에서 가장 많이 함유되어 있었으며, lactic acid, succinic acid 및 formic acid 순으로 함유되어 있었다. 잎과 줄기의 아미노산 조성은 각각 다르게 나타났지만 잎과 줄기의 주된 아미노산은 aspartic acid, glutamic acid, isoleucine 및 lysine으로 나타났다. 잎과 줄기의 주요 지방산은 linoleic acid와 palmitic acid 였다. To study the potential of the Humulus japonicus leaves and stalks as materials for functional food, chemical components of the male and female plants were analyzed. Among general components of leaves and stalks, contents of nitrogen free extract and crude protein were higher in leaves than in stalks, whole contents of moisture and crude fiber were higher in stalks than in leaves. The major minerals contained were K, Ca, P and Mg. Among free sugars, frutose(0.16% and 0.10%) and sucrose(0.37% and 0.24%) were the highest in leaves and stalks of male and female plants, respectively. Of non-volatile organic acids assayed acetic acid was the most abundant in both leaves(4.99 mg% and 3.07 mg%) and stalks(3.18 mg% and 2.25 mg%), followed by lactic acid, succinic acid and formic acid. The amino acid compositions of leaves and stalks were different each other and aspartic acid, glutamic acid, isoleucine and lysine were the major amino acids in both leaves and stalks. Major fatty acid in leaves and stalk were linoleic acid and palmitic acid.
최대하 운동부하검사가 노인 허혈성 뇌졸중환자의 혈관탄성·운동유발성 고혈압 및 허혈성 심장질환진단에 미치는 영향 : 사례연구
강동연,차재관,허재택,박정준,양점홍 釜山大學校 附設 體育科學硏究所 2009 體育科學硏究所 論文集 Vol.25 No.2
Purpose: The purpose of this study was to observe the blood pressure response during exercise test and to investigate baPWV and ABI and potentiality of the cardiovascular disease of three ischemic stroke elderly in submaximal exercise test. Method: It measured the blood pressure, ECG during submaximal exercise test and baPWV, ABI before and after exercise test. Results: It discovered low blood vessel elasticity, exercise induced hypertension, ST segment depression ≥ 1 mm through exercise test. Conclusion: Exercise test have a diagnosis valuable to decide a cardiac disorders. Moreover, It increases a sensibility of baPWV and ABI test and heighten a reliability of potential diagnosis of high blood pressure by submaximal exercise test. 뇌졸중(stroke)은 가령(加齡)과 함께 증가하며 크게 허혈성과 출혈성 형태로 니누고 근래에는 허혈성 뇌졸중의 발생빈도가 증가하고 있는 추세로 뇌졸중 환자의 혈류역학에 대한 연구는 순환기학 분야에서 뿐만 아니라 운동이 고혈압의 주요 개선 중재방법의 하나라는 관점에서 운동처방 분야에서도 심도 있는 연구가 요구된다. 그러므로 이 연구에서는 60세 이상의 허혈성 뇌졸중환자 3명에게 최대하 운동부하검사를 실시하여 각각의 사례에서 안정시에는 발견되지 않았던 혈관폐색의 높은 가능성, 운동유발성 고혈압 및 허혈성 심장질환의 정후인 ST분절≥1.0 mm 하강을 발견하였다. 이러한 점에서 운동부하검사는 심장질환을 판단하는 진단적 가치뿐만 아니라 baPWV와 ABI검사의 민감성을 증가시키며 고혈압 잠재성 진단에 유용하게 활용될 수 있다.
골격근 근형질세망의 ATPase활성도에 대한 Vanillylnonanamide의 영향
박경섭,홍장희,류영수,성지연,허강민,임종호,이재흔,석정호 충남대학교 의과대학 지역사회의학연구소 1999 충남의대잡지 Vol.26 No.2
To investigate the effect of vanillylnonanamide(VN) on the ATPase activity of the sarcoplasmic reticulum(SR) of the skeletal muscle, we prepared the SR vesicles from the back muscle of the rabbit, and measured ATPase activity. The results as follows: Ca-ATPase activity was about 50% in the total ATPase activity of skeletal muscular SR. In the reaction mixture with calcium, 100μM VN increased ATPase activity to 20%, and 100 nM and 10 μM thapsigargin(THP) inhibited the ATPase activity to 50% and 60%, respectively. And 100 μM VN plus 100 nM or 10 μM THP more inhibited ATPase activity than THP alone did it. However, without calcium, 100μM VN did not affect ATPase activity, and 10 μM THP inhibited it to 41%, but VN plus THP inhibited it to 31%. The above results show that VN in the reaction mixture with or without calcium has the different action to ATPase activity when it is used alone or together with Ca-ATPase inhibitor THP. This suggests that VN might increase or decrease the skeletal SR Ca-ATPase activity through affecting the lipid membrane around the Ca-ATPase.
Yeon-Jae Hur,Han-Gil Lee,Jong-Hun Choi,Gaosheng Hu,Eun-young Kim,Doh-Hoon Kim 한국작물학회 2007 한국작물학회 학술발표대회 논문집 Vol.2007 No.11
A phosphate starvation-induced acid phosphatase cDNA was cloned from the rice, Oryza sativa. The cDNA encoding O. sativa acid phosphatase (OsACP1) has 1100 bp with an open reading frame of 274 amino acid residues. The deduced amino acid sequence of OsACP1 cDNA showed 53% identity to tomato acid phosphatase and 46-50% identity to several other plant phosphatases. OsACP1 expression was up-regulated in the rice plant and in cell culture in the absence of phosphate (Pi). The induced expression of OsACP1 was a specific response to Pi starvation, and was not affected by the deprivation of other nutrients. OsACP1 expression was responsive to the level of Pi supply, with transcripts of OsACP1 being abundant in Pi-deprived root. The OsACP1 cDNA was expressed as a 30 kDa polypeptide in baculovirus-infected insect Sf9 cells. In addition, the OsACP1 gene was introduced into Arabidopsis via Agrobacterium mediated transformation. Functional expression of the OsACP1 gene in the transgenic Arabidopsis lines was confirmed by Northern blot and Western blot analyses, as well as phosphatase activity assays. These results suggest that the OsACP1 gene can be used to develop new transgenic dicotyledonous plants able to adapt to Pi-deficient conditions.
Mapping of qBK1, a major QTL for bakanae disease resistance in rice
Hur, Yeon-Jae,Lee, Sais Beul,Kim, Tae Heon,Kwon, Tackmin,Lee, Jong-Hee,Shin, Dong-Jin,Park, Soo-Kwon,Hwang, Un-Ha,Cho, Jun Hyeon,Yoon, Young-Nam,Yeo, Un-Sang,Song, You-Chun,Kwak, Do-Yeon,Nam, Min-Hee Springer-Verlag 2015 Molecular breeding Vol.35 No.2
QTL analysis of the qBK1, a major QTL for bakanae disease resistance in rice
Yeon-Jae Hur,Saes-Beul Lee,Tae-Heon Kim,Jong-Hee Lee,Dong-Jin Shin,Soo-Kwon Park,Woon-Ha Hwang,Sang-Ik Han,Jun-Hyun Cho,Young-Nam Yoon,Un-Sang Yeo,You-Chun Song,Min-Hee Nam,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Bakanae disease is one of the most serious and oldest problems of rice production, which was first described in 1828 in Japan (Ito and Kimura 1931). This disease may infect rice plants from the pre-emergence stage to the mature stage, with severe infection of rice seeds resulting poor germination or withering (Iqbal et al. 2011). Under favorable environmental conditions, infected plants have the capacity to produce numerous conidia that subsequently infect proximate healthy plants, resulting in major yield loss (Ou 1985). One hundred sixty nine NILs, YR28297 (BC6F4) generated by five backcrosses of Shingwang with the genetic background of susceptible japonica variety, Ilpum were used for QTL analysis. Rice bakanae disease pathogen, CF283, was mainly used in this study and inoculation and evaluation of bakanae disease was performed with the method of the large-scale screening method developed by Kim et al. (2014). A major QTL for resistance against bakanae disease on chromosome 1 was identified using SSR marker, RM9, which explaining 65 % of the total phenotype variation. The major QTL designated as qBK1 and mapped to a 4.4 Mbp region between RM24 (19.30 Mb) and RM11295 (23.72 Mb). The results of this study are expected to provide useful information toward developing resistant rice lines to this detrimental fungal disease.
Hur, Jae Uk,Han, Jin Soon,Shin, Jae Rok,Park, Hye-Yeong,Choi, Sung-Churl,Jung, Yeon-Gil,An, Gye Seok Elsevier 2019 CERAMICS INTERNATIONAL Vol.45 No.17
<P><B>Abstract</B></P> <P>Core–shell-structured SnO<SUB>2</SUB>-decorated Fe<SUB>3</SUB>O<SUB>4</SUB> nanoparticles were successfully synthesized by a simple solvothermal reaction. We propose an effective approach to decorate SnO<SUB>2</SUB> on the Fe<SUB>3</SUB>O<SUB>4</SUB> surface using trisodium citrate dihydrate (tSCD) and poly(acrylic acid) (PAA) as premodification materials acting as cross linkers and surface active agents. The modifications with tSCD and PAA introduced carboxyl groups on the surfaces of the Fe<SUB>3</SUB>O<SUB>4</SUB> nanoparticles, which affected their surface charges and dispersion properties in the reaction solution. Consequently, the anionic surface modification of Fe<SUB>3</SUB>O<SUB>4</SUB> provided uniformly decorated SnO<SUB>2</SUB> nanoparticles on the Fe<SUB>3</SUB>O<SUB>4</SUB> nanoparticles. SnO<SUB>2</SUB> bound to the surface of Fe<SUB>3</SUB>O<SUB>4</SUB> had different bonding behaviors depending on the surface modification method. The obtained Fe<SUB>3</SUB>O<SUB>4</SUB>@SnO<SUB>2</SUB> (tSCD- and PAA-modified) nanocomposites had atomic ratios of Sn of 5.62% and 3.97%, respectively. The observed difference could be attributed to the bonding structures and methods using tSCD and PAA. The surface properties and SnO<SUB>2</SUB> adsorption behaviors were analyzed in detail.</P>
Functional markers for bacterial blight resistance gene Xa3 in rice
Hur, Yeon Jae,Jeung, Ji-Ung,Kim, Sang Yeol,Park, Hyun-Soo,Cho, Jun-Hyun,Lee, Ji Yun,Sohn, Young-Bo,Song, You Chun,Park, Dong-Soo,Lee, Choon-Woo,Sohn, Jae Geun,Nam, Min-Hee,Lee, Jong Hee Springer-Verlag 2013 Molecular breeding Vol.31 No.4