Colonic Transit Disorder Mediated by Downregulation of Interstitial Cells of Cajal/Anoctamin-1 in Dextran Sodium Sulfate-induced Colitis Mice

Chen Lu,Hongli Lu,Xu Huang,Shaohua Liu,Jingyu Zang,Yujia Li,Jie Chen,Wenxie Xu 대한소화기 기능성질환∙운동학회 2019 Journal of Neurogastroenterology and Motility (JNM Vol.25 No.2

Background/Aims Interstitial cells of Cajal (ICC) and their special calcium-activated chloride channel, anoctamin-1 (ANO1) play pivotal roles in regulating colonic transit. This study is designed to investigate the role of ICC and the ANO1 channel in colonic transit disorder in dextran sodium sulfate (DSS)-treated colitis mice. Methods Colonic transit experiment, colonic migrating motor complexes (CMMCs), smooth muscle spontaneous contractile experiments, intracellular electrical recordings, western blotting analysis, and quantitative polymerase chain reaction were applied in this study. Results The mRNA and protein expressions of c-KIT and ANO1 channels were significantly decreased in the colons of DSS-colitis mice. The colonic artificial fecal-pellet transit experiment in vitro was significantly delayed in DSS-colitis mice. The CMMCs and smooth muscle spontaneous contractions were significantly decreased by 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB), an ANO1 channel blocker, and NG-Nitro-L-arginine methyl ester hydrochloride (L-NAME), an inhibitor of nitric oxide synthase activity, in DSS-colitis mice compared with that of control mice. Intracellular electrical recordings showed that the amplitude of NPPB-induced hyperpolarization was more positive in DSS-colitis mice. The electric field stimulation-elicited nitric-dependent slow inhibitory junctional potentials were also more positive in DSS-colitis mice than those of control mice. Conclusion The results suggest that colonic transit disorder is mediated via downregulation of the nitric oxide/ICC/ANO1 signalling pathway in DSS-colitis mice.

Effects of Components on the Photoelectric Properties of CuxO Nanotubes Fabricated by Using Thermal Oxidation of Copper Nanowires

Shaohua Chen,Xuan Liu,Shengyang Dai,Wenfeng Xiang,Haizhong Guo,Junjian Li 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.76 No.2

CuxO (Cupric Oxide) nanotubes (NTs) were synthesized by using thermal oxidation of Cu nanowires (NWs) for 2 and 4 hours, respectively, and the effect of the components on their photoelectrical properties due to the infrared (IR) light with a wavelength of 1064 nm were investigated. In the 2-h CuxO NTs, the main component was Cu2O; however, the 4-h CuxO NTs were composed of a mixture of CuO and Cu2O. The photoelectric properties showed that the voltage difference and the sensitivity of 4-h CuxO NTs were more than 3 times those of the 2-h CuxO NTs, and its linear range was only about one-third that of the 2-h CuxO NTs. The above results were caused by the 4-h CuxO NTs containing a higher content of CuO, which had a suitable bandgap of 1.2 eV for light with a wavelength of 1064 nm. In addition, the response and the recovery times for the 4-h NTs were on the same order as those of the 2-h NTs. The conclusions from this article indicated that 4-h and 2-h NTs were very promising for the design of IR photodetector device with higher sensitivity and a larger detection range respectively.

Efficient degradation of imidacloprid in wastewater by a novel p-n heterogeneous Ag2O/BiVO4/diatomite composite under hydrogen peroxide

Jing Chen,Qifang Ren,Chunshan Xu,Bin Chen,Shaohua Chen,Yi Ding,Zhen Jin,Wanmi Guo,Xinyu Jia 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

In this study, Ag2O/BiVO4/diatomite composite with p-n heterojunction structure was synthesized bysimple hydrothermal method with diatomite as carrier. It was found that under the action of hydrogenperoxide (H2O2), the imidacloprid (IMD) could be effectively degraded by the composite. Systemic evaluationswas conducted on effects of pH value, catalyst dosage, H2O2 concentration, initial IMD concentration,and anion on how effeciently this new photocatalytic composite can degrade IMD. The results showthat the p-n heterojunction formed between the two contact surfaces of Ag2O nanoparticle and BiVO4promotes the charge transfer between the interfaces, inhibits recombination of electrons and holes,and thus significantly improves the catalytic performance. The stability and reusability of Ag2O/BiVO4/diatomite composites were tested with ICP experiment and cyclic experiment. Active substancecapture experiment and ESR reveal the photocatalytic reaction mechanism and confirm that superoxideradical (O2), hydroxyl radical (OH), and h+ can promote the degradation of IMD. The degradation productsand the intermediates of IMD were identified through LC-MS, on a basis of which the possible transformationpathway was proposed. In situ synthesis of p-type Ag2O and n-type BiVO4 heterojunctioncomposites has opened a new direction for photocatalytic degradation of organic pollutants.

Study on flow fields of centrally fuel rich swirl burner and its applications

Zhichao Chen,Zhengqi Li,Jianping Jing,Lizhe Chen,Shaohua Wu,Yang Yao 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.5

Experiments on a single-phase test facility were done to optimize primary air outlet cones of a centrally fuel rich swirl coal combustion burner. On the basis of optimized results from the single-phase test, a three-component particle-dynamics anemometer was used to measure, in the near-burner region, the characteristics of gas/particle two-phase flows for the burner with two primary air outlet cones, on a gas/particle two-phase test facility. Velocities, RMS velocities and particle volume flux profiles were obtained. According to the results, the primary air outlet cone structure of the centrally fuel rich burner was matching a 670 ton per hour boiler. The performance of the burner on a 670 ton per hour boiler was studied.

A Compressive Signal Detection Scheme Based on Sparsity

Shaohua Qin,Dongyan Chen,Xu Huang,Leilei Yu 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.2

SLAMF7 is critical for phagocytosis of haematopoietic tumour cells via Mac-1 integrin

Chen, Jun,Zhong, Ming-Chao,Guo, Huaijian,Davidson, Dominique,Mishel, Sabrin,Lu, Yan,Rhee, Inmoo,Pé,rez-Quintero, Luis-Alberto,Zhang, Shaohua,Cruz-Munoz, Mario-Ernesto,Wu, Ning,Vinh, Donald C.,Si Nature Publishing Group 2017 Nature Vol. No.

<P>Cancer cells elude anti-tumour immunity through multiple mechanisms, including upregulated expression of ligands for inhibitory immune checkpoint receptors(1,2). Phagocytosis by macrophages plays a critical role in cancer control(3-6). Therapeutic blockade of signal regulatory protein (SIRP)-alpha, an inhibitory receptor on macrophages, or of its ligand CD47 expressed on tumour cells, improves tumour cell elimination in vitro and in vivo(7-10), suggesting that blockade of the SIRP alpha-CD47 checkpoint could be useful in treating human cancer(11-14). However, the prophagocytic receptor(s) responsible for tumour cell phagocytosis is(are) largely unknown. Here we find that macrophages are much more efficient at phagocytosis of haematopoietic tumour cells, compared with non-haematopoietic tumour cells, in response to SIRP alpha-CD47 blockade. Using a mouse lacking the signalling lymphocytic activation molecule (SLAM) family of homotypic haematopoietic cell-specific receptors, we determined that phagocytosis of haematopoietic tumour cells during SIRP alpha-CD47 blockade was strictly dependent on SLAM family receptors in vitro and in vivo. In both mouse and human cells, this function required a single SLAM family member, SLAMF7 (also known as CRACC, CS1, CD319), expressed on macrophages and tumour cell targets. In contrast to most SLAM receptor functions(15-17), SLAMF7-mediated phagocytosis was independent of signalling lymphocyte activation molecule-associated protein (SAP) adaptors. Instead, it depended on the ability of SLAMF7 to interact with integrin Mac-1 (refs 18-20) and utilize signals involving immunoreceptor tyrosine-based activation motifs(21,22). These findings elucidate the mechanism by which macrophages engulf and destroy haematopoietic tumour cells. They also reveal a novel SAP adaptor-independent function for a SLAM receptor. Lastly, they suggest that patients with tumours expressing SLAMF7 are more likely to respond to SIRP alpha-CD47 blockade therapy.</P>

Damage detection using the improved Kullback-Leibler divergence

Shaohua Tian,Xuefeng Chen,Zhibo Yang,Zhengjia He,Xingwu Zhang 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.48 No.3

Structural health monitoring is crucial to maintain the structural performance safely. Moreover, the Kullback-Leibler divergence (KLD) is applied usually to asset the similarity between different probability density functions in the pattern recognition. In this study, the KLD is employed to detect the damage. However the asymmetry of the KLD is a shortcoming for the damage detection, to overcoming this shortcoming, two other divergences and one statistic distribution are proposed. Then the damage identification by the KLD and its three descriptions from the symmetric point of view is investigated. In order to improve the reliability and accuracy of the four divergences, the gapped smoothing method (GSM) is adopted. On the basis of the damage index approach, the new damage index (DI) for detect damage more accurately based on the four divergences is developed. In the last, the grey relational coefficient and hypothesis test (GRCHT) is utilized to obtain the more precise damage identification results. Finally, a clear remarkable improvement can be observed. To demonstrate the feasibility and accuracy of the proposed method, examples of an isotropic beam with different damage scenarios are employed so as to check the present approaches numerically. The final results show that the developed approach successfully located the damaged region in all cases effect and accurately.

Personality Characteristics of Male Sufferers of Chronic Tension-Type and Cervicogenic Headache

Wanzhen Chen,Shaohua Yu,Junpeng Zhu,Hao Chai,Wei He,Wei Wang 대한신경과학회 2012 Journal of Clinical Neurology Vol.8 No.1

Background and Purpose Chronic tension-type headache (a primary headache disorder)and cervicogenic headache (a secondary headache disorder that is attributable to upper cervical spine pathology) share similar clinical manifestations, but their associated personality traits may differ. We evaluated the personality differences between sufferers of chronic tension-type headache and cervicogenic headache. Methods We administered the Zuckerman-Kuhlman Personality Questionnaire (ZKPQ) and the Zuckerman Sensation-Seeking Scale (SSS) to 18 patients suffering from chronic tensiontype headache, 19 suffering from cervicogenic headache, and 26 healthy volunteers. Depressive trends were measured with the Plutchik-van-Praag Depression Inventory (PVP). Results Compared to healthy controls, the chronic tension-type headache group scored significantly higher on ZKPQ Neuroticism-Anxiety and on the PVP, while the cervicogenic headache group scored significantly lower on SSS Thrill and Adventure Seeking. In addition, the total SSS score was significantly lower in the cervicogenic headache group than in both the chronic tension-type headache group and the healthy controls. Conclusions The results of this study indicate that higher scores for neuroticism-anxiety and depression were associated with chronic tension-type headache, while lower sensation-seeking scores were associated with cervicogenic headache.

Damage detection using the improved Kullback-Leibler divergence

Tian, Shaohua,Chen, Xuefeng,Yang, Zhibo,He, Zhengjia,Zhang, Xingwu Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.48 No.3

Structural health monitoring is crucial to maintain the structural performance safely. Moreover, the Kullback-Leibler divergence (KLD) is applied usually to asset the similarity between different probability density functions in the pattern recognition. In this study, the KLD is employed to detect the damage. However the asymmetry of the KLD is a shortcoming for the damage detection, to overcoming this shortcoming, two other divergences and one statistic distribution are proposed. Then the damage identification by the KLD and its three descriptions from the symmetric point of view is investigated. In order to improve the reliability and accuracy of the four divergences, the gapped smoothing method (GSM) is adopted. On the basis of the damage index approach, the new damage index (DI) for detect damage more accurately based on the four divergences is developed. In the last, the grey relational coefficient and hypothesis test (GRCHT) is utilized to obtain the more precise damage identification results. Finally, a clear remarkable improvement can be observed. To demonstrate the feasibility and accuracy of the proposed method, examples of an isotropic beam with different damage scenarios are employed so as to check the present approaches numerically. The final results show that the developed approach successfully located the damaged region in all cases effect and accurately.

The penalized Fischer-Burmeister SOC complementarity function

Pan, Shaohua,Chen, Jein-Shan,Kum, Sangho,Lim, Yongdo Springer-Verlag 2011 Computational optimization and applications Vol.49 No.3