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- 저자 : ( Li Sha Xin ) (검색결과 13 건)
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Xu, Xin-fang,Cheng, Xian-long,Lin, Qing-hua,Li, Sha-sha,Jia, Zhe,Han, Ting,Lin, Rui-chao,Wang, Dan,Wei, Feng,Li, Xiang-ri The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.4
Background: Mountain-cultivated ginseng (MCG) and cultivated ginseng (CG) both belong to Panax ginseng and have similar ingredients. However, their pharmacological activities are different due to their significantly different growth environments. Methods: An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS)-based approach was developed to distinguish MCG and CG. Multivariate statistical methods, such as principal component analysis and supervised orthogonal partial-least-squares discrimination analysis were used to select the influential components. Results: Under optimized UPLC-QTOF-MS/MS conditions, 40 ginsenosides in both MCG and CG were unambiguously identified and tentatively assigned. The results showed that the characteristic components of CG and MCG included ginsenoside Ra3/isomer, gypenoside XVII, quinquenoside R1, ginsenoside Ra7, notoginsenoside Fe, ginsenoside Ra2, ginsenoside Rs6/Rs7, malonyl ginsenoside Rc, malonyl ginsenoside Rb1, malonyl ginsenoside Rb2, palmitoleic acid, and ethyl linoleate. The malony ginsenosides are abundant in CG, but higher levels of the minor ginsenosides were detected in MCG. Conclusion: This is the first time that the differences between CG and MCG have been observed systematically at the chemical level. Our results suggested that using the identified characteristic components as chemical markers to identify different ginseng products is effective and viable.
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Li Bai-Jian,Fu Wen-Qiang,Fu Xin-Sha,Huang Yan 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.4
Two laboratory tests were conducted to investigate the mechanical properties and develop a load-carrying capacity estimation method for reinforced concrete (RC) slab culverts rehabilitated with a grouted corrugated steel plate (CSP). Subsequently, 216 numerical models of RC slab culverts rehabilitated with different shapes of CSPs and grout strengths were established to investigate the influence of these parameters and the arch effect on the rehabilitated system. A mechanical model was proposed based on the elastic center method, and a load-carrying capacity estimation method of RC slab culverts rehabilitated with grouted CSPs was established and verified. It was concluded that the load-carrying capacity of the rehabilitated system increased with a decrease in the radius of the side walls and crown at a constant radius of the CSP haunch. At a constant radius of the side walls and CSP crown, the load-carrying capacity of the rehabilitated system increased with an increase in the haunch radius. The most effective way to improve the load-carrying capacity of the rehabilitated system was to increase the radius of the haunches and reduce the radius of the arch crown and side wall. The arch effect of the grout was related to the load type. The load-carrying capacity of the rehabilitated system was the highest when the CSP was similar to or the same as the arch axes of the grout. The most important function of the grout was to provide strong lateral restraint for the CSP, reducing the required span and improving the load-carrying capacity of the CSP. In addition, the shear strength of the grout contributed to improving the load-carrying capacity by exerting an arch effect or experiencing shear failure. The proposed load-carrying capacity estimation method is applicable to rehabilitated systems with a box or arch-type CSP. Our findings provide guidance for engineers to design similar rehabilitated systems.
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Novel impedance matching method based on negative resistors for WPT
Li, Yang,Liu, Jiaming,Jiang, Shan,Ni, Xin,Ma, Jingnan,Wang, Rui,Sha, Lin The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.4
In a wireless power transfer (WPT) system via coupled magnetic resonance, the transmission power and efficiency dramatically decrease with increased in the transfer distance and load variations. In the conventional impedance matching method, different resistors are matched by adjusting passive devices such as capacitors and inductors. The corresponding matching range changes with different topological network structures. In particular, there exists a matching forbidden zone. In this paper, a novel impedance matching method based on a negative resistor is proposed to match the impedance in the forbidden zone. First, the conventional impedance matching network is analyzed based on circuit theory. Then, the feasibility of the proposed method is verified. In addition, the receiving power obtained using the conventional matching method is compared with that obtained using the proposed method. It is theoretically verified that the novel method can break the upper power limit related to the conventional method, which realizes a higher receiving power. A prototype was built in the laboratory. Experimental results show the feasibility of the novel method. Moreover, derived simulation results were basically consistent with the experimental values, which also demonstrates the validity of the proposed method.
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Non-leaching and durable antibacterial textiles finished with reactive zwitterionic sulfobetaine
Liang He,Chang Gao,Sha Li,Cordelia T.W. Chung,John H. Xin 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.46 No.-
An antibacterialfinishing protocol using a reactive sulfobetaine was reported to fabricate safe anddurable antibacterial textiles. The specifically designed sulfobetaine contains highly reactive triazinegroup acting as an anchor tofix the antibacterial betaine group on textiles via covalent bond formation. After thefinishing, the textiles were endowed durable antibacterial activities of 98.0% against gramnegativeEscherichia coli and 95.2% against gram-positive Staphylococcus aureus even after they werelaundered for 30 times. The safety evaluation showed that the reactive sulfobetaine had no skin irritationand cytotoxicity. These results indicate that the developed antibacterialfinishing is safe and durable ontextiles.
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Selective extraction of thorium to directly form self-assembly solid from HNO3 solution
Fang Zhang,Qiang Wu,Lei-Tao Sha,Yang Li,Xu-Xin Li,Ze-Yang Wang,Xuan Fu,Qing-Gang Huang,Bin Liu,Ze-Yi Yan 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-
Based on ions exchange between [DMDSA]+[Cl]- (Dimethyl distearyl ammonium chloride) and N,Ndialkyl-succinamide acid (SCA), three novel bifunctional [DMDSA]+[SCA]- ionic liquids (ILs) were firstlysynthesized for extraction of thorium (IV) by self-assembly strategy. The simultaneous extraction andsolidification of Th(IV) were unexpectedly realized in one-step operation using the present ILs in HNO3solution, and more than 99% thorium (IV) was enriched and immediately aggregated into selfassemblysolid at the biphasic interface. The self-assembly solid was further identified by FT-IR, SEM withelement mapping EDS and XPS analysis, and revealing that the self-assembly extraction (SAE) was triggeredby the amphiphilic [DMDSA]+ cations. A three-step extraction mechanism dominated by [SCATh(NO3)4]- was proposed based on the slope analysis method and HRMS analysis. The self-assembly extractionof Th(IV) exhibited the extremely excellent selectivity in the presence of U(VI) and typical lanthanideelements including La(III), Eu(III) and Lu(III), and the separation factors reached 2516 for Th/U, 1885 forTh/La, 1512 for Th/Eu and 558 for Th/Lu, respectively. The proposed SAE strategy was proved to be anefficient method for one-step separation and solidification of thorium ions from U(VI) and/or lanthanides.
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Zhao Yu Han,Zheng Yu,Sha Jie,Hua Hong Jin,Li Ke Dong,Lu Yu,Dang Yi Ni,Zhang Guo Xin 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.1
Background/Aims: The discrepancies between the diagnosis of preoperative endoscopic forceps biopsy (EFB) and endoscopic submucosal dissection (ESD) in patients with early gastric neoplasm (EGN) exist objectively. Among them, pathological upgrading directly influences the accuracy and appropriateness of clinical decisions. The aims of this study were to investigate the risk factors for the discrepancies, with a particular focus on pathological upgrading and to establish a prediction model for estimating the risk of pathological upgrading after EFB. Methods: We retrospectively collected the records of 978 patients who underwent ESD from December 1, 2017 to July 31, 2021 and who had a final histopathology determination of EGN. A nomogram to predict the risk of pathological upgrading was constructed after analyzing subgroup differences among the 901 lesions enrolled. Results: The ratio of pathological upgrading was 510 of 953 (53.5%). Clinical, laboratorial and endoscopic characteristics were analyzed using univariable and binary multivariable logistic regression analyses. A nomogram was constructed by including age, history of chronic atrophic gastritis, symptoms of digestive system, blood high density lipoprotein concentration, macroscopic type, pathological diagnosis of EFB, uneven surface, remarkable redness, and lesion size. The C-statistics were 0.804 (95% confidence interval, 0.774 to 0.834) and 0.748 (95% confidence interval, 0.664 to 0.832) in the training and validation set, respectively. We also built an online webserver based on the proposed nomogram for convenient clinical use. Conclusions: The clinical value of identifying the preoperative diagnosis of EGN lesions is limited when using EFB separately. We have developed a nomogram that can predict the probability of pathological upgrading with good calibration and discrimination value.
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Zhang Jing,Wang Hong,Yi Sha,Guo Zemei,Huang Yue,Li Weifeng,Zhao Xin,Liu Huazhi 한국응용생명화학회 2020 Applied Biological Chemistry (Appl Biol Chem) Vol.63 No.3
In this study, Insect tea primary leaf (Malus sieboldii (Regal) Rehd.) was used as the research object to investigate the protective effect of Insect tea primary extract (ITPLE) on hydrogen peroxide (H2O2)-induced oxidative damage in human embryonic kidney 293T cells (HEK 293T cells) and the mechanism of action of the main active components. The 3-(4,5-dimethyl-2-thiazolyl)- 2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was used to determine the toxicity of ITPLE to HEK 293T cells in vitro as well as its protective effect against (H2O2)-induced oxidative damage in HEK 293T cells. In addition, various assay kits were used to measure oxidation-related indicators in HEK 293T cells, and quantitative polymerase chain reaction (qPCR) analysis was used to determine the mRNA expression levels of oxidation-related genes in HEK 293T cells. High performance liquid chromatography (HPLC) analysis was used to characterize active components in ITPLE. The experimental results revealed that the ITPLE had no toxic effect on cells in the range of 0–200 μg/mL, and, in this range, exhibited a concentration-dependent protective effect against H2O2- induced oxidative damage in HEK 293T cells. It was also found that the ITPLE can reduce the malondialdehyde (MDA) level and increase the levels of superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-Px), and catalase (CAT)in oxidative damage HEK 293T cells. The qPCR analysis results also showed that the ITPLE upregulated the mRNA expression levels of SOD, CAT , GSH and GSH-Px in HEK 293T cells damaged by H2O2- induced oxidative stress. The HPLC analysis identified 7 bioactive components in the ITPLE, including neochlorogenic acid, cryptochlorogenic acid, rutin, kaempferin, isochlorogenic acid B, isochlorogenic acid A and hesperidin. This study reveals that ITPLE is rich in active compounds and has good antioxidant effect in vitro, thus it has the potential to be developed into a traditional Chinese medicine and functional drinks.
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The K+/H+ antiporter AhNHX1 improved tobacco tolerance to NaCl stress by enhancing K+ retention
Wei-Wei Zhang,Jing-Jing Meng,Jin-Yi Xing,Sha Yang,Feng Guo,Xin-Guo Li,Shu-Bo Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.3
High salinity is the one of important factors limiting plant growth and crop production. Many NHX-type antiporters have been reported to catalyze K+/H+ exchange to mediate salt stress. This study shows that an NHX gene from Arachis hypogaea L. has an important role in K+ uptake and transport, which affects K+ accumulation and plant salt tolerance. When overexpressing AhNHX1, the growth of tobacco seedlings is improved with longer roots and a higher fresh weight than the wild type (WT) under NaCl treatment. Meanwhile, when exposed to NaCl stress, the transgenic seedlings had higher K+/H+ antiporter activity and their roots got more K+ uptake. NaCl stress could induce higher K+ accumulation in the roots, stems, and leaves of transgenic tobacco seedlings but not Na+ accumulation, thus, leading to a higher K+/Na+ ratio in the transgenic seedlings. Additionally, the AKT1, HAK1, SKOR, and KEA genes, which are involved in K+ uptake or transport, were induced by NaCl stress and kept higher expression levels in transgenic seedlings than in WT seedlings. The H+-ATPase and H+-PPase activities were also higher in transgenic seedlings than in the WT seedlings under NaCl stress. Simultaneously, overexpression of AhNHX1 increased the relative distribution of K+ in the aerial parts of the seedlings under NaCl stress. These results showed that AhNHX1 catalyzed the K+/H+ antiporter and enhanced tobacco tolerance to salt stress by increasing K+ uptake and transport.
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