A study of nerve agent model organophosphonate binding with manganese-A₂B-corrole and -A₂B₂-porphyrin systems

Kim, K.,Kim, I.,Maiti, N.,Kwon, S.J.,Bucella, D.,Egorova, O.A.,Lee, Y.S.,Kwak, J.,Churchill, D.G. Pergamon Press 2009 Polyhedron Vol.28 No.12

Herein the synthesis and binding studies of novel trans-A<SUB>2</SUB>B-corrole and trans-A<SUB>2</SUB>B<SUB>2</SUB>-porphyrin derivatives are presented in comparing manganese(III)-organophosphonate (OP) binding (e.g., M<SUP>n+</SUP>←O?PR(OR)<SUB>2</SUB>) capabilities. H<SUB>3</SUB>(PFP-VC) [PFP-VC=5,15-di(pentafluorophenyl)-10-(3-vinylphenyl)corrolate] was synthesized by way of literature procedures and was characterized by a variety of 2-D NMR spectroscopic techniques and single-crystal X-ray diffraction. These compounds represent the first example of 3-vinyl-phenyl-containing meso-substituted corroles or porphyrins. Mn(PFP-VC) (3) was treated separately with (CH<SUB>3</SUB>CH<SUB>2</SUB>O)<SUB>2</SUB>P?O(C<SUB>3</SUB>H<SUB>6</SUB>NMe<SUB>2</SUB>), (C<SUB>4</SUB>H<SUB>9</SUB>O)<SUB>2</SUB>P?O(Me), (C<SUB>2</SUB>H<SUB>5</SUB>O)<SUB>2</SUB>P?O(CH<SUB>2</SUB>COCH<SUB>3</SUB>), (CH<SUB>3</SUB>CH<SUB>2</SUB>O)<SUB>2</SUB>P?O(Me), to give 1:1 adducts, as determined by UV-Vis spectroscopy (Job Plot), giving a red shift; Ph<SUB>3</SUB>P?O, was also found to bind, but very weakly. The trans-A<SUB>2</SUB>B<SUB>2</SUB>-porphyrin analogue Mn(PFP-VP) (4) was also prepared by way of a literature procedure; related binding studies gave 1:1 organophosphonate-Mn(PFP-VP) adducts (Job Plot). A clean blue shift occurred for the Mn-porphyrins at higher organophosphonate loadings (K<SUB>a</SUB> values: 6.7 (0.9)-11.9 (0.4)M<SUP>-1</SUP>). DFT geometry optimizations of O?P(OMe)<SUB>2</SUB>Me binding and formal Mn-O or P-O cleavage products in the unsubstituted neutral Mn-corrolato and -porphyrinato systems with a range of metal-based spin states revealed greatest stability in formal phosphoryl oxygen binding (energies: 11-13kcal/mol) for the Mn-corrole (singlet); the Mn-porphyrin (sextet) was also quite stable.

RF-O₂ Plasma 처리한 MgO 박막의 스퍼터링 수율 측정

정원희(W. H .Jeoung),정강원(K. W. Jeong),임연찬(Y. C. Lim),오현주(H. J. Oh),박철우(C. W. Park),최은하(E. H. Choi),서윤호(Y. H. Seo),김윤기(Y. K. Kim),강승언(S. O. Kang) 한국진공학회(ASCT) 2006 Applied Science and Convergence Technology Vol.15 No.3

RF-O₂ plasma 처리한 MgO 박막의 스퍼터링 수율을 집속이온빔 장치를 이용하여 측정하였다. 가속 전압 10 ㎸의 Ga 이온빔을 주사했을 때 plasma 처리하지 않은 MgO 박막의 스퍼터링 수율은 0.33 atoms/ion, RF-O₂ plasma 처리한 MgO 박막의 스퍼터링 수율은 0.20 atoms/ion 으로 RF-O₂ plasma 처리한 경우 스퍼터링 수율이 낮아졌다. 또한 XPS, AFM을 통해 plasma 처리로 인한 MgO 표면의 변화를 관찰하였다. MgO 박막에 RF-O₂ plasma 처리 한 후 XPS O 1s spectra의 binding energy와 FWHM 값이 각각 2.36 eV와 0.6167 eV 작아졌고 표면거칠기의 RMS 값 또한 0.32 ㎚ 작아졌다. We measured sputtering yield of RF O₂-plasma treated MgO protective layer for AC-PDP(plasma display panel) using a Focused Ion Beam System(FIB). A 10 ㎸ acceleration voltage was applied. The sputtering yield of the untreated sample and the treated sample were 0.33 atoms/ion and 0.20 atoms/ion, respectively. The influence of the plasma-treatment of MgO thin film was characterized by XPS and AFM analysis. We observed that the binding energy of the O 1s spectra, the FWHM of O 1s spectra and the RMS(root-mean-square) of surface roughness decreased to 2.36 eV, 0.6167 eV and 0.32 ㎚, respectively.

Combustion properties of gaseous CH₄/O₂ coaxial jet flames in a single-element combustor

Kim, T.Y.,Choi, S.,Kim, H.K.,Jeung, I.S.,Koo, J.,Kwon, O.C. Butterworths [etc.] ; Elsevier Science Ltd 2016 Fuel Vol.184 No.-

Fundamental combustion properties of gaseous methane (CH<SUB>4</SUB>)/oxygen (O<SUB>2</SUB>) coaxial jet flames in a single-element combustor are experimentally evaluated as a preliminary step for subsequent studies of injection at very low temperature or using liquid O<SUB>2</SUB> for CH<SUB>4</SUB>/O<SUB>2</SUB> bipropellants, recently appearing to be the oncoming liquid bipropellant. A combustion chamber with quartz windows, a single shear coaxial injector and an exhaust nozzle on the downstream of the chamber is considered for the present study. Focusing on the measurements of the ignition and combustion stability limits of the coaxial jet flames in the chamber, flame visualization is also conducted by OH<SUP>*</SUP> chemiluminescence, schlieren imaging and direct imaging. Results show the ignition limits restricted than the combustion stability limits. Flame behaviors are largely classified into two, the stably attached flame and the oscillating, liftoff (near-blowout) flame. Due to cooling effects on the wall of the chamber, stably liftoff flame is not observed. The stability of the flame is greatest at fuel-rich condition (based on the injected amounts of CH<SUB>4</SUB> and O<SUB>2</SUB>). From the flame visualization flame thickness is found to be smaller than the injector lip thickness and insensitive to injection conditions. The laminar-flow behavior near the injector exit due to the strong burning of pure O<SUB>2</SUB> is observed even for high Reynolds numbers (Re). The flame visualization also exhibits the recirculating O<SUB>2</SUB> that enhances burning in the combustor through the reaction with the outer fuel jet. The results of ignition limits, combustion stability limits and flame visualization can be used as a database for researches of modeling the gaseous CH<SUB>4</SUB>/O<SUB>2</SUB> jet flames in the combustion chamber.

抗生物質의 製劑學的 硏究(Ⅴ) : Al. stearate와 Eudragit함량에 따른 Eudragit-암피실린 마이크로캅셀의 제조에 대한 연구 Studies on effect of Al.stearate and Eudragit contents in preparation of Eudragit-ampicillin microcapsules

김기경,김인규,김도훈,윤미옥,장정윤,김영림,최선옥,이경희,정기선,김종국 국립보건연구원 1994 국립보건원보 Vol.31 No.2

Integrin αvβ3-mediated transcriptional regulation of TIMP-1 in a human ovarian cancer cell line

Kim, D.S.,Jeon, O.H.,Lee, H.D.,Yoo, K.H.,Kim, D.S. Academic Press 2008 Biochemical and biophysical research communication Vol.377 No.2

We have previously reported that a disintegrin inhibits solid tumor growth and metastasis in mouse model [I.C. Kang, Y.D. Lee, D.S. Kim, A novel disintegrin salmosin inhibits tumor angiogenesis, Cancer Res. 59 (1999) 3754-3760; S.I. Kim, K.S. Kim, H.S. Kim, D.S. Kim, Y. Jang, K.H. Chung, Y.S. Park, Inhibitory effect of the salmosin gene transferred by cationic liposomes on the progression of B16BL6 tumors, Cancer Res. 63 (2003) 6458-462]. In this study, we have investigated the modulatory effect of a disintegrin, saxatilin, on the balance between MMP-9 and tissue inhibitor of metalloproteinase-1 (TIMP-1) in human ovarian cancer cell line MDAH 2774. Functional mechanism of the disintegrin-mediated transcriptional regulation of MMP-9 and TIMP-1 was examined in the ovarian cancer cell line. Saxatilin strongly induced TIMP-1 expression in dose- and time-dependent manners, while the disintegrin suppressed MMP-9 expression. Further analyses clearly indicated that interaction of the disintegrin and integrin αvβ3 results in the TIMP-1 promoter activation via c-fos to suppress TNF-α-induced cancer cell invasion. These results demonstrate that integrin αvβ3-mediated transcriptional regulation of MMP-9 and TIMP-1 is critical for suppressing the ovarian cancer cell invasion.

CHF₃ / C₂F6 플라즈마에 의한 실리콘 표면 잔류막의 특성

권광호(K.-H. Kwon),박형호(H.-H. Park),이수민(S. M. Lee),강성준(S. J. Kang),권오준(O.-J. Kwon),김보우(B.W. Kim),성영권(Y.-K. Sung) 한국진공학회(ASCT) 1992 Applied Science and Convergence Technology Vol.1 No.1

실리콘을 CHF₃/C₂F_6 가스 플라즈마를 이용하여 식각하면 실리콘위에 탄소, 불소 및 산소로 이루어진 잔류막이 형성된다. 이 잔류막을 XPS로 분석한 결과 탄소는 C-Si, C-Si, C-C/H, C-CF_x(x≤3), C-F, C-F₂, C-F₃ 결합을 하고 있으며, 불소는 F-Si, F-C 및 F-O 결합으로 이루어져 있음을 알았다. 한편 산소는 O-Si 및 O-F 결합으로, 실리콘은 Si-Si, Si-C 및 Si-O 결합상태를 나타낸다. 잔류막의 수직분포 연구를 통하여 Si-O 및 Si-C 결합이 탄소와 불소의 결합층 아래에 존재하고, 잔류막의 표면부에 F-O 결합이 분포함을 알았다. 또한 건식식각 변수가 잔류막 형성에 미치는 영향이 조사되었으며 CHF₃/C₂F_6 가스 유량비, RF power 벚 압력 등이 잔류막의 두께, 조성비 및 잔류막의 결합상태에 영향을 미침을 알 수 있었다. Si surfaces exposed to CHF₃/C₂F_6 gas plasmas in reactive ion etching (RIE) have been characterized by X-ray photoelectron spectroscopy (XPS). CHF₃/C₂F_6 gas plasma exposure of Si surface leads to the deposition of residual film containing carbon and fluorine. The narrow scan spectra of C 1s show various bonding states of carbon as C-Si, C-Si, C-C/H, C-CF_x(x≤3), C-F, C-F₂, and C-F₃. The chemical bonding states of fluorine are described with F-Si, F-C, and F-O. And the oxygen and silicon are also detected. The effects of parameters for reactive ion etching as CHF₃/C₂F_6 gas ratio, RF power, and pressure are investigated.

Self-standing and shape-memorable UV-curing epoxy polymers for three-dimensional (3D) continuous-filament printing

Sun, H.,Kim, Y.,Kim, Y. C.,Park, I. K.,Suhr, J.,Byun, D.,Choi, H. R.,Kuk, K.,Baek, O. H.,Jung, Y. K.,Choi, H. J.,Kim, K. J.,Nam, J. D. The Royal Society of Chemistry 2018 Journal of materials chemistry. C, Materials for o Vol.6 No.12

<P>In the development of three-dimensional printable materials for high-speed and high-resolution printing, UV-curing polymers can guarantee fast and precise printing of high performance load-bearing structures, but the injected drops of the monomers tend to spread over the substrates due to their low viscosity. In this study, we imposed the self-standing and shape-memorable capability of an epoxy acrylate (EA) monomer to ensure continuous filamentary 3D printing while maintaining its low viscosity nature. Using octadecanamide (ODA) with EA, strong hydrogen-bond networks (−N−H⋯OC−, −N−CO⋯H-O-, -N-H⋯N-) were additionally achieved in the material system and the developed material distinctively exhibited rheological duality at different processing stages: a low-viscosity liquid-like behavior (viscosity of ∼50 Pa) while passing through the nozzle and a self-standing solid-like behavior (static yield stress of ∼364 Pa) right after being printed. This reversible liquid-to-solid transitional capability was quantified by viscoelastic complex moduli provided a dynamic yield stress (<I>τ</I>y,G) of 210 Pa corresponding to the upright stacking up to ∼3.2 cm (3 wt% of ODA). The time (<I>t</I>y,G) required for conformational rearrangement was evaluated to be as fast as ∼10<SUP>−2</SUP> s. After UV curing, the 3D printed layers exhibited no air pockets or weld lines at the stacked interfaces, which could guarantee excellent mechanical performance and structural integrity.</P>

첨가제를 첨가한 빙축열시스템 PCM용 TMA-포접화합물의 과냉도 개선에 대한 연구

김진흥,정낙규,김창오,강승현,김광일 朝鮮大學校 機械技術硏究所 2003 機械技術硏究 Vol.6 No.1

TMA clathrate that is used as PCM of low temperature thermal storage system in this research creates hydrate crystallization at higher temperature than pure water, and its application is expected a s PCM because of comparatively big latent heat without phase separation phenomenon Acetone is used as additive and evaluated experimentally for the purpose of the improvement in subcooling of TMA clathrate. In view of the results so far achieved subcooling is improved, the running time of the refrigerator is reduced. Thus the results re expected to use for the increase of coefficient of performance of ].ow temperature thermal storage system in the building.

돼지 卵子의 透明帶에 대한 單一클론抗體生産과 그 特性에 關한 硏究

金鐘培,劉永春,金昌圭,權五中,鄭盛元,鄭吉生 건국대학교 동물자원연구센터 1991 動物資源硏究誌 Vol.16 No.-

本 試驗은 單一클론抗體의 강한 特異性과 抗體性質의 不變性을 이용하여 發生學的 側面에서 哺乳動物 卵子의 透明帶의 機能과 構造를 이해하고, 또한 種特異的인 精子 受容體의 存在 및 生化學的 構造를 규명하기 위한 기초연구로서, 돼지 卵子의 透明帶를 免疫原으로 하여 BABL/c 생쥐로부터 單一클론抗體를 생산하고 그 특성을 구명하였던 바 다음과 같은 결과를 얻었다. 1) 3마리의 BABL/c 생쥐(YⅠ, YⅡ, ZI)에 돼지卵子의 透明帶를 免疫化하고, 複合抗體 生成을 확인한 후 생쥐의 脾臟細胞와 Myeloma(SP2/O-Ag14)를 polyethylene glycol를 融合을 실시한 결과 각각 25.8%, 54.5% 그리고 59.7%의 融合效率을 나타내었으며, ELISQ에 의해 陽性反應을 조사한 결과 각각 17.3%, 32.6% 그리고 6.2% 陽性反應 效率을 나타내었다. 2) YI에서 강한 陽性反應을 보인 6개의 well에 대한 cloning을 실시하고 抗體檢證을 행한 결과 20.8% ∼ 48.4%의 Cloning效率과 54.6% ∼ 82%의 陽性反應 效率을 나타내었다. 3) 강한 陽性反應을 나타낸 항체에 대해 sandwich ELISA法에 의해 isotype을 決定하였던바 2E93C(YⅠ), 3E83E7(YⅠ), 4E3(YⅡ)각각 IgG₂b, IgG₂a, IgM으로 확인되었다. 4) Isotype이 決定된 2E93C9(YⅠ), 3E84E7(YⅠ), 4E3(YⅡ)의 세포를 생쥐의 腹腔에 주사하여 얻은 腹水를 indirect ELISA法에 의해 titer를 決定한 결과 모두 1:400,000 이상의 높은 titer를 나타내었다. 5) 處理區로서 單一클론抗體의 腹水와 對照區로서 normal mouse serum이 각각 2%씩 함유된 배양액속에서 난자를 배양한 후 顯徵鏡下에서 관찰했을 때 對照區에서 배양된 난자의 표면은 정상적인 형태를 나타냈으나 處理區에서 배양된 卵子는 표면에 뚜렷한 沈澱層을 형성하였다. 6) 處理區와 對照區 卵子를 Rabbit anti-mouse IgG-FITC가 1% 함유된 배양액속에서 배양하고 洗滌한 후 螢光顯徵鏡下에서 관찰한 바 處理區의 卵子는 透明帶 주위에서 螢光이 나타났으나, 對照區에서는 나타나지 않았다. This study was carried out ot produce and characterize monoclonal antibodies against porcine zona pellucida, and undertaken as a basic study to develop immunocontraceptive vaccine and to investigate the function of zana pellucida in early fertilization process. The results obtained were summarized as follows: 1. Spleen cells of three BALB/C mice(YⅠ, YⅡ and ZI) which were immunized with porcine zona pelucida were fused with myeloma cells(SP2/O-Ag14) by polyethylene glycol. At the result of fusion, fusion efficiency was 25.8 , 54.5% and 59.7% and positive efficiency 17.3%, 32.6% and 6.2%, respectively. 2. Cloning efficiency was shown to be from 20.8% to 48.4% and positive efficiency of them were 54.6% to 82%. 3. Sub-isotypes of three strong positive antibodies, 2E93C(YⅠ), 3E83E7(YⅠ) and 4E3(YⅡ) were determined by sandwich ELISA method and shown to be as IgG2b, IgH2a and IgM, respectively. 4. The titers of three ascitic fluids containing antibodies, 2E93C9(YⅠ), 3E84E7(YⅠ) and 4E3(YⅡ) were determined by indirect ELISA and all of them showed above 1:400,000. 5. The layer of precipitate was observed on the zona incubated with medium containing 2% ascitic fluid of monoclonal antibody while the eggs treated with 2% normal mouse serum did not. 6. Porcine eggs incubated with medium containing 2% ascitic fluid of monoclonal antibody and followed by subsequent incubation with Rabbit anti-mouse IgG-FITC conjugate showed strong fluorescent light on the zona surface while the zona of normal mouse serum-treated eggs did not show fluorescence.

HIV 항체 효소면역시험에 대한 정도평가 분석

김성순,서순덕,김옥진,이민우,김현숙,김영란,박광일,황병갑,이용래,조연정,황유성,이주실 국립보건연구원 2000 국립보건원보 Vol.37 No.-