제지공정수 최적화를 위한 UF의 적합성 평가 및 응용

김형진,이지영,권혁윤 한국공업화학회 2004 응용화학 Vol.8 No.1

This study was carried out to evaluate the application possibility of UF Membrane System to papermaking industry in order to optimize the recycling of white water. In this study, for the efficiency test of UF modules, the MWCO(molecular weight cut-off) of the membrane was estimated by the COD measurement from UF filtrate water. From the rejection rate of membrane, three kinds of UF module were selected and evaluated in the factors of COD, SS, turbidity and flow rate.

돼지 미성숙 난포란의 회수 및 체외수정에 관한 연구

박성원,김정혁,홍승표,이지삼,정장용,박희성 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

본 연구는 돼지의 체외수정에 있어서 난포란의 회수방법과 체외수정란 및 체외발달율을 조사하여 체외수정의 기본 원리를 밝히고자 본 연구를 수행하였다. 도축장으로부터 채집한 난소의 난포에서 흡입법, 세절법 및 흡입후세절법 등으로 난포란을 채취하여 등급별로 분류하여 회수율을 조사하였으며, 채취한 난포란은 액상정액과 정소상체미부 정액을 사용하여 체외수정을 시킨후 TCM-199 와 NCSU-23 배양액으로 체외배양을 실시하였다. 본 연구에서 얻은 결과를 요약하면 다음과 같다. 회수방법당 각각 37개의 난소에서 회수한 난포란의 수는 흡입법이 1,365개, 세절법이 1,884개 및 흡입후 세절법이 3.830개로써 난소당 난포란의 수는 각각 36.9, 50.9 및 103.5개로써 회수방법간에 유의적(P<0.05)인 차이가 있었다. 액상정액과 정소상체미부 정액을 사용하였을 때 수정율은 각각 83.0 및 83.% 로써 정액간에 유의적(P<0.05) 인 차이는 없었다. 체외수정이 이루어진 난자들의 분할율도 액상정액(60.8%) 과 정소상체미부 정액(69.0%) 간에 유의적(P<0.05) 인 차이는 없었다. 2-4세포기로의 발달율은 24.6 및 28.4%로써 배양액간에 위의적(P<0.05)인 차이는 없었으며, 상실배로의 발달율도 TCM-199 와 NCSU-23 배양액이 각각 14.8 및 24.5% 로써 이들간에 유의적(P<0.05)인 차이는 없었다. To improve the efficiency of in vitro production of embryos with follicular oocytes in pig, the recovery rates, in vitro fertilization and development, and the time required for collectng and processing oocytes by aspiration with or without slicing were evaluated comparatively. The oocytes were collected by aspiration of follicles(2-6mm) with or without slicing ovaries after aspiration, and classified into grade I-IV oocytes by the morpholoy of cumulus cells attached and the homogeneity of cytoplasmic granules. The grade Ⅰ-Ⅱ oocytes were matured invitro for 46 hours in TCM-199 and NCSU-23 supplemented with 1㎍/㎖ FSH, 2IU/㎖ hCG, 1㎍/㎖ estradiol-17 at 39℃ under 5% CO_2 in air. They were fertilized in vitro by epididymis sperm and ejaculated sperm treated with hypotaurin for 24 hours. and then the zygotes were co-cultured in vitro with pFF(porcine follicular fluid) for 7-9 days. The results obtained were as follows: The number of ocytes recovered per ovary was averaged 30.7 by aspiration, 50.8 by slicing and 103.5 by aspiration post slicing. The number of grade Ⅰ oocytes recovered was total 0.2% by aspiration, 0.1% by slicing and 0.1% by aspiration post slicing. The oocytes recovered per ovary was significantly(P<0.05) higher as recovery methods. The cleavage rates of the embryos obtained from 83.0(ejaculate sperm) and 83.1(epididymis sperm) respectively(P<0.05). In vitro developmental rates(9.9 and 18.6%) of embryos cultured in TCM-199 and NCSU-23 up to morula stage were non significantly(P<0.05) different between the culture media.

하악골에 발생된 Low grade 골육종:증례보고

조현이,정종철,김호성,최재욱,성대경,서지훈,김성범,이계혁 大韓顎顔面成形再建外科學會 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.1

Osteosarcoma is a primary malignant tumor arising most often in the long bone, but rare in jaw bone. Especially osteogenic sarcoma of the mandible is a rare tumor, comprising less than 0.5% of all head and neck tumors. Osteosarcoma occurs cheifly in young persons, and presenting very survival rates. Histopathologically this tumor can be classified three types, osteoblastic, fibroblastic, chondroblastic and classified from low grade to high grade by anaplasia or mitosis of the tumor cells. Sometimes, the exact diagnosis of osteosarcoma is difficult especially in low grade osteosarcoma, even if clinical or radiographical findings suggest to osteosarcoma. So suspcious to malignant bone tumor in clinical or radiological findings, biopsy should be taked from deep portion and multiple area, and sharing the patient history and radiographs with pathologist will assist in the development of the diagnosis. We report a case of low grade osteosarcoma on the mandible initially difficulty in accurate diagnosis. The patient visited our hospital for routine dental treatment but radiographic findings displayed ill-defined radiolucency with osteoid formation on the mandible. Final diagnosis was difficulty in initial biopsy but subsequent biopsy taked from deep portion presented infiltrative growth and mitosis of the tumor cell in some area with small osteoid bone formation, so we could reach final diagnosis as low grade sarcoma. We treated this patient with bloc resection of the mandible and immediate reconstruction with iliac corticocancellous block.

Tumor suppressor p53 plays a key role in induction of both tristetraprolin and <i>let-7</i> in human cancer cells

Lee, Ji Young,Kim, Hyo Jeong,Yoon, Nal Ae,Lee, Won Hyeok,Min, Young Joo,Ko, Byung Kyun,Lee, Byung Ju,Lee, Aran,Cha, Hee Jeong,Cho, Wha Ja,Park, Jeong Woo Oxford University Press 2013 Nucleic acids research Vol.41 No.11

<P>Tristetraprolin (TTP) and <I>let-7</I> microRNA exhibit suppressive effects on cell growth through down-regulation of oncogenes. Both TTP and <I>let-7</I> are often repressed in human cancers, thereby promoting oncogenesis by derepressing their target genes. However, the precise mechanism of this repression is unknown. We here demonstrate that p53 stimulated by the DNA-damaging agent doxorubicin (DOX) induced the expression of <I>TTP</I> in cancer cells. TTP in turn increased <I>let-7</I> levels through down-regulation of <I>Lin28a</I>. Correspondingly, cancer cells with mutations or inhibition of p53 failed to induce the expression of both <I>TTP</I> and <I>let-7</I> on treatment with DOX. Down-regulation of <I>TTP</I> by small interfering RNAs attenuated the inhibitory effect of DOX on <I>let-7</I> expression and cell growth. Therefore, TTP provides an important link between p53 activation induced by DNA damage and <I>let-7</I> biogenesis. These novel findings provide a mechanism for the widespread decrease in TTP and <I>let-7</I> and chemoresistance observed in human cancers.</P>

Highly selective fluorescence imaging of zinc distribution in HeLa cells and <i>Arabidopsis</i> using a naphthalene-based fluorescent probe

Lee, Ji Ha,Lee, Jin Hyeok,Jung, Sung Ho,Hyun, Tae Kyung,Feng, Mingxiao,Kim, Jae-Yean,Lee, Jae-Hong,Lee, Hoyeon,Kim, Jong Seung,Kang, Chulhun,Kwon, Ki-Young,Jung, Jong Hwa The Royal Society of Chemistry 2015 Chemical communications Vol.51 No.35

<P>2-(<I>N</I>,<I>N</I>-Dimethylamino)naphthalene-based probe <B>1</B> was found to exhibit a dramatic enhancement in fluorescence upon addition of Zn<SUP>2+</SUP>, but not with any other metal ions. Probe <B>1</B> as a chemoprobe enabled high-resolution fluorescence imaging of zinc ions in HeLa cells and <I>Arabidopsis</I>.</P> <P>Graphic Abstract</P><P>2-(<I>N</I>,<I>N</I>-Dimethylamino)naphthalene-based probe <B>1</B> was found to exhibit a dramatic enhancement in fluorescence upon addition of Zn<SUP>2+</SUP>, but not with any other metal ions. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5cc01364j'> </P>

Liposoluble portion of the red alga Pyropia yezoensis protects alcohol induced liver injury in mice

Ji-Hyeok Lee,Ginne Ahn,Juyoung Ko,Nalae Kang,Kyungsook Jung,Eui-Jeong Han,Gwang Hoon Kim,Hee Jeong Kim,Cheol Soo Choi,You-Jin Jeon 한국조류학회I 2021 ALGAE Vol.36 No.3

The hepatoprotective effect of liposoluble portion of Pyropia yezoensis (PYLP) was investigated against alcohol-induced liver injury in mice. Fatty acids were predominant in PYLP obtained from hexane fraction of 70% EtOH extract after ultrasonication. In particular, polyunsaturated fatty acids such as eicosapentaenoic acid and linoleic acid accounted for 56.91% of the total lipids. PYLP significantly reduced liver damage induced by the alcohol treatment in mice. PYLP treatment increased the activity of antioxidant enzymes including superoxide dismutase, catalase, and glutathion peroxidase by reducing thiobarbituric acid reactive substances. Histological observations showed that PYLP minimizes damage to living tissue induced by alcohol treatment by modulating the expression level of proteins involved in the anti-apoptotic signaling pathway. Our results suggest that PYLP, rich in polyunsaturated fatty acids extracted from the red alga P. yezoensis, will be useful as a potential liver protectant in the hangover industry.

Fibronectin gene expression in human adipose tissue and its associations with obesity-related genes and metabolic parameters.

Lee, Seoung Hee,Park, Hye Soon,Lee, Jung Ah,Song, Young Sook,Jang, Yeon Jin,Kim, Jong-Hyeok,Lee, Yeon Ji,Heo, Yoonseok Rapid Communications of Oxford 2013 Obesity surgery Vol.23 No.4

<P>Limited data are available on the in vivo expression of fibronectin, one of the main extracellular matrix components. We investigated the expression of fibronectin in abdominal visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) and the associations of leptin, adiponectin, and vaspin gene expression with metabolic parameters in obese women.</P>

A benzothiazole-based receptor-immobilised silica nanoparticle as chemosensor for Hg²⁺

Lee, Jin Hyeok,Lee, Ji Ha,Kim, Sung Kuk,Jung, Jong Hwa Informa UK (TaylorFrancis) 2015 Supramolecular chemistry Vol.27 No.10

Screening and Characterization of Novel Promoters from Pichia pastoris

Hyeok-Won Lee,Ji-yeon Hong,Jung-Oh Ahn,Dong-Heui Yi,Eui-Sung Choi,Joon-Ki Jung,Hong-Weon Lee 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.4

Impact of Maternal Age on Pregnancy Outcomes in Primiparous Women in South Korea

( Ji Eun Lee ),( Yeon Sung Jung ),( Ji Sun Yoon ),( Jae Eun Chung ),( Eui Hyeok Kim ),( Sang A Lee ) 대한산부인과학회 2020 대한산부인과학회 학술대회 Vol.106 No.-

Objective: The elderly primigravida is defined as a woman who gets pregnant for the first time at the age of 35 years or older. It is well known that elderly primigravida is a high-risk pregnancy having an increased probability of maternal complications besides fetal genetic risks. This study aims to determine and compare the pregnancy outcomes in elderly primigravidae (EP) aged 35 years or older with those of young primigravidae (YP) aged below 35 years in South Korea. Methods: Using the 2013-2018 administrative database from National Health Insurance claim data, we reviewed medical information of women who had their first singleton delivery with taking the National Health Screening Examination to identify baseline patients' characteristics. We compared the prevalence of each high-risk pregnancy diagnosis as defined by ICD-10 codes, the rate of Cesarean delivery, and other pregnancy complications including postpartum hemorrhage and readmission after delivery between EP and YP. Results: Among the 548,080 women, 106,178 were women were 35 or more years old (EP) and 441,902 were under 35 (YP). EP were more likely than YP to be obese (11.8% vs. 17.2%, p<0.001), pre-existing DM and Hypertension (0.18% vs. 0.53%, 0.25% vs.0.86%, all p <0.001). Multivariate analysis showed that EP was independently associated with DM/GDM using insulin, preeclampsia, and preterm birth (adjusted odds ratio [aOR] 2.29, 95% confidence intervals (CI): 2.15-2.45, aOR 1.60, 95% CI: 1.36-1.88, aOR 1.44, 95% CI: 1.38-1.51, respectively). EP was also positively associated with cesarean delivery and low birth weight (aOR 1.72, 95% CI 1.70-1.75, aOR 1.74, 95% CI: 1.66-1.82). EP could be the risk factor for hospitalization before delivery, transfusion and readmission after delivery too. Conclusion: EP is independently associated with higher risks of a range of adverse outcomes. Timely and more vigilance is needed both for obstetricians and EP.