플라보노이드의 세포 신호전달 조절

이응룡,강근호,강용진,김우열,최혜연,김봉우,정효순,조쌍구 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

Many studies revealed the neuroprotective, cardioprotective, and chemopreventive actions of dietary flavonoids. The plausible mechanistic interpretation of the various effects of flavonoids was concentrated on the anti-oxidant or free radical-scavenging properties of these phytochernicals, both in model systems and under in vivo conditions. While there has been a major focus on the anti-oxidant properties. there is an emerging view that flavonoids and their in vivo metabolites. do not act as conventional hydrogen-donating anti-oxidants. but they may exert regulatory functions in cells through actions at protein kinase or lipid kinase signaling pathways. Flavonoids and more recently their metabolites. have been reported to act at phosphoinositide 3-kinase(PI 3-kinase). Akt/protein kinase B(Akt/PKB), protein kinase C (PKC), mitogen activated protein kinase(MAP kinase), and various tyrosine kinases signaling cascades. Inhibitory or stimulatory actions at these pathways are likely to affect cellular function profoundly by altering the phosphorylation state of target molecules and by modulating gene expression. A clear understanding of the mechanisms of action of flavonoids, either as anti-oxidants or modulators of cellular signaling pathways, and the influence of their metabolism on these properties are key to the evaluation of these potent biomolecules as anti-cancer agents, cardio-protectants, and inhibitors of neurodegeneration.

한강 하류 김포평야의 최종빙기 이래 퇴적환경변화

황상일,김혜령,윤순옥 경북대학교 사회과학대학 지리학과 2003 地理學論究 Vol.- No.23

한강하류부에 위치한 김포시 고촌면 김포평야의 충적충과 사면퇴적충에서 퇴적상 분석 및 화분 분석을 실시하여 식생환경, 해면변동, 기후변화와 같은 자연환경인화를 검토하고 이것을 선사시대 인간활동과의 관계에서 살펴보았다. 최종빙기 이래 형성된 연구지역의 퇴적상은 빙기의 선상지성사면퇴적층과 흘로세 충적충으로 구분되며, 화분분석은 흘로세 충적충의 유기질이 다소 포함된 회색실트충, 분해되지 않은 토탄충, 분해된 토탄충에서 이루어졌다. 화분조성은 흘로세 중기 이후 해면미변동을 반영하며, 화분대GP-Ⅰ은 흘로세 해진의 영향으로 수심이 있는 저습지 환경에서 Alnus를 중심으로 한 목본 우점기에 퇴적되었고, 화분대GP-ll시기는 해면의 지속적인 정체와 하강에 따른 지하수위 저하 그리고 활발해진 인간활동의 영향으로 목본이 크게 줄고, 포자와 초본류가 우점하게 되었다. The environmental change such as vegetation environment, sea-level change, climate change and human impact was restored at Gimpo alluvial plain on the upper Han river according to the results of sedimentary facies and pollen analysis. The sedimentary facies which has been constructed since the last glacial stage is composed of the hillslope deposits as the alluvial fan during the glacial stage and Holocene alluvium. Materials for pollen analyses were sampled from gray organic silt layer, peat I layer(abundant fibroid material included the branches of trees and roots of grasses) and peat Ⅱ layer(decomposed peat including organic clay) in the alluvium of the Holocene. The change of the pollen composition reflected sea-level change and vegetation environment during the Holocene. Pollen zone GP-Ⅰ was the period of AP-dominance, including mostly Alnus. Study area was under the lagoon or swamp environment influenced by Flandrian transgression. Pollenzone Ⅱ was the period of Spore & NAP-dominance. It represents that vegetation environment was changed to drier condition by falling the surface of ground-water caused by sea-level regression after long-term stability, and influenced of human activity.

Case report of diseases in psittacine birds, South Korea, 2012-2021

Hye-Ryoung Kim(Hye-Ryoung Kim),Chung-Hyun Kim(Chung-Hyun Kim),Hye-Sun Song(Hye-Sun Song),Ji-Ye Kim(Ji-Ye Kim),Jae-Kyeom Kim(Jae-Kyeom Kim),Yong-Kuk Kwon(Yong-Kuk Kwon) 한국예방수의학회 2022 한국예방수의학회 학술대회자료집 Vol.2022 No.-

Potential of Cross-infection of Colletotrichum Species Causing Anthracnose in Persimmon and Pepper

Kim, Hye-Ryoung,Lim, Tae-Heon,Kim, Joo-Hyung,Kim, Young-Ho,Kim, Heung-Tae The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.1

Ninety isolates of Colletotrichum species from new persimmon tree twigs and 50 isolates from pepper plant fruits were isolated via single-spore isolation. Of the 140 isolates, 26 were examined for mycelial growth, carbendazim sensitivity, and ITS sequence. Four of the isolates from the persimmon trees, which were cultivated exclusively in an orchard, showed fast mycelial growth and sensitivity to carbendazim, while five of the pepper isolates showed slower mycelial growth and were resistant to the fungicide. However, 17 isolates from persimmon trees cultivated with pepper plants in the same orchard showed slow mycelial growth like the pepper isolates and they were sensitive to carbendazim like the persimmon isolates. ITS sequence analysis of these 27 isolates led to the identification of the 22 persimmon isolates as C. gloeosporioides and the five pepper isolates as C. acutatum. PCR with species-specific primers confirmed that the 90 isolates from persimmon were C. gloeosporioides whereas the 50 isolates from pepper were C. acutatum. The 90 persimmon isolates of C. gloeosporioides and 50 pepper isolates of C. acutatum were compared by a wound inoculation test to determine their capacity for host cross-infection. All of the C. acutatum isolates from pepper caused typical symptoms of anthracnose on the fruits of pepper plants and twigs of persimmon; they differed from the C. gloeosporioides isolates from persimmon, more than 90% of which were able to infect only persimmon. Amplified fragment length polymorphism analysis revealed the existence of two groups (C. gloeosporioides and C. acutatum isolates group). At 80% genetic similarity, the C. gloeosporioides group was defined within four clusters, while the C. acutatum group was within three clusters. However, these clusterings were unrelated with the virulence of Colletotrichum species against pepper fruits.

Studies on Constituents of the Higher Fungi of Korea (XLVI) : Antitumor Components Extracted from the Cultured Mycelia of Lyophyllum decastes

Hye Ryoung Kim,Mi Ja Shim,Jung Woo Kim,Ha Won Kim,Chong Ock Lee,Eung Chil Choi,Byong Kak Kim 한국생약학회 1984 생약학회지 Vol.15 No.2

To investigate antitumor component of Lyophyllum decastes, the aqueous extract of its shake-cultured mycelia was subjected to antitumor test against sarcoma 180 cells implanted in ICR mice. The extract showed an inhibition ratio of 65.4% and was found to consist of a polysaccharide moiety and a protein moiety. After purification with DEAE-Sephadex A-50 ion exchange chromatography, Fraction D showed the highest inhibition ratio of 75.7%. The antitumor constituent was examined for immunoaccelerating activity and was found to increase macrophage accumulation in the peritoneal cavity and plaque forming cells of the spleen cells. It was named lyophyllan after the genus name.

Family caregivers’ perceived value of caring for older patient: A hybrid model of concept analysis

Seon-Hye Heo(Seon-Hye Heo),Hye-Ryoung Kim(Hye-Ryoung Kim) 한국노인간호학회 2023 노인간호학회지 Vol.25 No.2

Purpose: The purpose of this study was to define and clarify the concept of the family caregivers’ perceived value of caring for older patients. Methods: The hybrid model of Schwartz-Barcott and Kim was used to perform the concept analysis of perceived value of caring. Concept analysis was conducted in three phases: the theoretical phase, fieldwork phase, and final analysis phase. Results: The perceived value of caring was found to have four dimensions and nine attributes. The providing care dimension consisted of two attributes—care behavior and care attitude—and the goals and expectation dimension included three attributes—social norms, family norms, and personal norms. The other two dimensions consisted of two attributes each: a belief in usefulness and a belief in meaning in the building beliefs dimension and life expansion and insight into the future in the suffering sublimation dimension. Finally, perceived value of caring can be defined as the personal belief that behavior or attitude of providing physical, mental, and social services to those in need of care is usefulness and important based on one’s goals and criteria, and with the personal effort of sublimating suffering that arises from care. Conclusion: Based on the study findings, it is suggested that the use of the perceived value of caring should expand in nursing phenomena.

Adiponectin receptor agonist AdipoRon decreased ceramide, and lipotoxicity, and ameliorated diabetic nephropathy

Choi, Sun Ryoung,Lim, Ji Hee,Kim, Min Young,Kim, Eun Nim,Kim, Yaeni,Choi, Beom Soon,Kim, Yong-Soo,Kim, Hye Won,Lim, Kyung-Min,Kim, Min Jeong,Park, Cheol Whee Elsevier 2018 clinical and experimental Vol.85 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Adiponectin is known to take part in the regulation of energy metabolism. AdipoRon, an orally-active synthetic adiponectin agonist, binds to both adiponectin receptors (AdipoR)1/R2 and ameliorates diabetic complications. Among the lipid metabolites, the ceramide subspecies of sphingolipids have been linked to features of lipotoxicity, including inflammation, cell death, and insulin resistance. We investigated the role of AdipoRon in the prevention and development of type 2 diabetic nephropathy.</P> <P><B>Methods</B></P> <P>AdipoRon (30 mg/kg) was mixed into the standard chow diet and provided to <I>db/db</I> mice (<I>db</I> + AdipoRon, <I>n</I> = 8) and age-matched male <I>db/m</I> mice (<I>dm</I> + AdipoRon, <I>n</I> = 8) from 17 weeks of age for 4 weeks. Control <I>db/db</I> (<I>db</I> cont, <I>n</I> = 8) and <I>db/m</I> mice (<I>dm</I> cont, <I>n</I> = 8) were fed a normal diet of mouse chow.</P> <P><B>Results</B></P> <P>AdipoRon-fed <I>db/db</I> mice showed a decreased amount of albuminuria and lipid accumulation in the kidney with no significant changes in serum adiponectin, glucose, and body weight. Restoring expression of adiponectin receptor-1 and -2 in the renal cortex was observed in <I>db/db</I> mice with AdipoRon administration. Consistent up-regulation of phospho-Thr<SUP>172</SUP> AMP-dependent kinase (AMPK), peroxisome proliferative-activated receptor α (PPARα), phospho-Thr<SUP>473</SUP> Akt, phospho-Ser<SUP>79</SUP>Acetyl-CoA carboxylase (ACC), and phospho-Ser<SUP>1177</SUP> endothelial NO synthase (eNOS), and down-regulation of protein phosphatase 2A (PP2A), sterol regulatory element-binding protein-1c (SREBP-1c), and inducible nitric oxide synthase (iNOS) were associated within the same group. AdipoRon lowered cellular ceramide levels by activation of acid ceramidase, which normalized ceramide to sphingosine-1 phosphate (S1P) ratio. In glomerular endothelial cells (GECs) and podocytes, AdipoRon treatment markedly decreased palmitate-induced lipotoxicity, which ultimately ameliorated oxidative stress and apoptosis.</P> <P><B>Conclusions</B></P> <P>AdipoRon may prevent lipotoxicity in the kidney particularly in both GECs and podocytes through an improvement in lipid metabolism, as shown by the ratio of ceramide to sphingosines, and further contribute to prevent deterioration of renal function, independent of the systemic effects of adiponectin. The reduction in oxidative stress and apoptosis by AdipoRon provides protection against renal damage, thereby ameliorating endothelial dysfunction in type 2 diabetic nephropathy.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Superfluous ceramide via decrease in acid ceramidase activity causes lipotoxicity. </LI> <LI> Adiponectin and adiponectin receptors potentially increase ceramidase activity. </LI> <LI> AdipoRon ameliorated renal lipotoxicity by AdipoR1-AMPK and AdipoR2-PPARα pathway. </LI> <LI> AdipoRon is a promising tool for treatment of diabetic nephropathy in type 2 DM. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

Effect of exercise interventions on sarcopenic obesity in middle-aged and older adults: a comprehensive review

Hye-Ryoung Kim 한국기초간호학회 2023 Journal of korean biological nursing science Vol.25 No.4

Purpose: This study examined the definitions, diagnostic criteria, and measurements of sarcopenic obesity and identified effective exercise interventions that improve cardiometabolic outcomes in middle-aged and older adults, in whom the prevalence of sarcopenic obesity is increasing. Methods: This comprehensive review followed the principles of literature search, data extraction, and review, as described in the PRISMA 2009 guidelines. Results: The 11 articles included in this study presented different concepts of sarcopenic obesity. Exercise interventions for sarcopenic obesity varied in their effects. Resistance exercise improved muscle mass and physical function, while aerobic exercise primarily impacted obesity and cardiometabolic indicators. Combined exercise had mixed results across indicators. Conclusion: This study addressed a pressing public health concern in the context of an aging population, acknowledged the unique challenges of sarcopenic obesity, and attempted to clarify definitions and assessment methods, while identifying effective exercise interventions to reduce cardiometabolic risk. Sarcopenic obesity is a multifaceted condition with varying definitions and diagnostic criteria. Its association with cardiometabolic risk underscores the need for comprehensive assessments considering both muscle and obesity indicators. While exercise interventions hold promise for managing sarcopenic obesity, further research is required to establish effective strategies.

Restriction fragment length polymorphism analysis of multiple genome regions of Korean isolates of infectious laryngotracheitis virus collected from chickens

Kim, Hye-Ryoung,Kang, Min-Su,Kim, Mi-Jin,Lee, Hee-Soo,Kwon, Yong-Kuk Poultry Science Association 2013 Poultry science Vol.92 No.8

<P>This study was conducted to characterize infectious laryngotracheitis (ILT) viruses isolated from poultry in South Korea using RFLP analysis of PCR products. Seven wild-type Korean isolates from commercial chicken farms collected between 1986 and 2012 were compared with 3 imported commercial vaccine strains [LT Blen (Hudson strain, United States), Laryngo Vac (Cover strain, United States), and Nobilis ILT (Serva strain, France)] and a Korean chicken embryo origin (CEO) vaccine strain [ILT-VAC (Gyeonggi97 strain, Korea)]. Six of the field isolates were highly virulent viruses, and the Kr12AD37 isolate was considered an attenuated type according to Han’s RFLP method. These virulent Korean ILT viruses were divided into 3 classes (class I, II, and III). The Kr12AD37 isolate was found to have the same RFLP pattern as the Korean CEO vaccine strain, and both of these strains were different from the 3 foreign vaccine strains. The results suggest that the Korean CEO vaccine strain has been responsible for recent outbreaks, and the characterization of ILT viruses by RFLP was useful for diagnosis by providing epidemiological information.</P>

Autonomy‐supportive, Web‐based lifestyle modification for cardiometabolic risk in postmenopausal women: Randomized trial

Kim, Hye‐,Ryoung,Kim, Hee‐,Seung unknown 2017 NURSING AND HEALTH SCIENCES Vol.19 No.4

<P><B>Abstract</B></P><P>The management of cardiometabolic risk factors, such as abdominal obesity, dyslipidemia, hypertension, and hyperglycemia, is essential for the health of postmenopausal women. In this study, we identified the effects of autonomy‐supportive, Web‐based lifestyle modification for the management cardiometabolic risk of postmenopausal women, and assessed the mediation effect of intrinsic motivation. This study was a randomized trial involving 71 postmenopausal Korean women. For the intervention group, we provided the Web‐based autonomy supports for 12 weeks; however, for the control group, individual consultations on healthy lifestyle was conducted at the first meeting. In the intervention group, cardiometabolic risks ameliorated as follows: waist circumference and waist‐to‐height ratio decreased by 3.9 cm and 0.03 cm, respectively; triglycerides decreased by 8.5 mg/dl; triglycerides‐to‐high‐density‐lipoprotein cholesterol ratio decreased by 0.21; systolic blood pressure decreased by 3.3 mmHg, and the visceral adiposity index decreased. Among the subdomain of intrinsic motivation, perceived competence, effort/importance, and perceived choice showed an association with waist circumference changes. Web‐based autonomy supports can be effective in implementation and maintenance, and the amelioration of cardiometabolic risk in postmenopausal women.</P>