Steel Bridge Construction of Hong Kong–Zhuhai–Macao Bridge

Wen-bo Gao,Quan-ke Su,Jin-wen Zhang,Hong-bing Xie,Feng Wen,Fang Li,Ji-zhu Liu 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.5

The 55-km-long Hong Kong–Zhuhai–Macao Bridge (HZMB) is the world’s longest sea-crossing bridge, connecting Hong Kong with Zhuhai and Macao at the mouth of the Pearl River Estuary in China, comprising 22.9-km-long steel bridges. HZMB is the leading steel bridge in China, with top-level manufacturing and installation technology. This paper outlines the steel bridge construction experiences of HZMB to provide comparisons for the construction of other long sea-crossing steel bridges at home or abroad. The main considerations of construction constraints, scheme selection, structural and aesthetic design of HZMB are presented, and the following points related to new strategies in the steel bridge construction of HZMB are elaborated: (1) construction quality assurance, (2) automatic manufacturing technology, (3) large segment off shore installation, (4) eco-friendly paint (content limitation of volatile organic compounds) and new multifunctional inspection gantry, and (5) Guss Mastic Asphalt steel deck pavement system. The successful implementation of those strategies shows that the steel bridge construction of HZMB promotes improvement in the overall construction and management level of the Chinese bridge industry. The advanced experience of HZMB has opened up broad prospects for the design and construction of off shore bridge engineering in China.

Ginsenoside Ro, an oleanolic saponin of Panax ginseng, exerts antiinflammatory effect by direct inhibiting toll like receptor 4 signaling pathway

Hong-Lin Xu,Guang-Hong Chen,Yu-Ting Wu,Ling-Peng Xie,Zhang-Bin Tan,Bin Liu,Hui-Jie Fan,Hong-Mei Chen,Gui-Qiong Huang,Min Liu,Ying-Chun Zhou 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.1

Background: Panax ginseng Meyer (P. ginseng), a herb distributed in Korea, China and Japan, exerts benefits on diverse inflammatory conditions. However, the underlying mechanism and active ingredients remains largely unclear. Herein, we aimed to explore the active ingredients of P. ginseng against inflammation and elucidate underlying mechanisms. Methods: Inflammation model was constructed by lipopolysaccharide (LPS) in C57BL/6 mice and RAW264.7 macrophages. Molecular docking, molecular dynamics, surface plasmon resonance imaging (SPRi) and immunofluorescence were utilized to predict active component. Results: P. ginseng significantly inhibited LPS-induced lung injury and the expression of proinflammatory factors, including TNF-a, IL-6 and IL-1b. Additionally, P. ginseng blocked fluorescence-labeled LPS (LPS488) binding to the membranes of RAW264.7 macrophages, the phosphorylation of nuclear factor-kB (NF-kB) and mitogen-activated protein kinases (MAPKs). Furthermore, molecular docking demonstrated that ginsenoside Ro (GRo) docked into the LPS binding site of toll like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD2) complex. Molecular dynamic simulations showed that the MD2-GRo binding conformation was stable. SPRi demonstrated an excellent interaction between TLR4/MD2 complex and GRo (KD value of 1.16 × 10<SUP>-9</SUP> M). GRo significantly inhibited LPS488 binding to cell membranes. Further studies showed that GRo markedly suppressed LPS-triggered lung injury, the transcription and secretion levels of TNF-α, IL-6 and IL-1β. Moreover, the phosphorylation of NF-kB and MAPKs as well as the p65 subunit nuclear translocation were inhibited by GRo dose-dependently. Conclusion: Our results suggest that GRo exerts anti-inflammation actions by direct inhibition of TLR4 signaling pathway.

Application of an Improved DV-HOP Algorithm in WSN

Hong Xie 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.11

As the DV-Hop algorithm in wireless sensing does not perform well in terms of accuracy, a DV-Hop sensor node location algorithm based on the Steffensen iteration method is presented in this paper. First, the wireless sensor nodes are located roughly through the DV-Hop algorithm; then, the location result is checked and corrected with the Steffensen iteration method, to realize accurate location. The simulation experiment shows that the node location accuracy is improved significantly by using the algorithm presented in this paper, which is more widely applicable.

Overexpression of SIRT2 contributes tumor cell growth in hepatocellular carcinomas

Hong Jian Xie,정광화,남석우 대한독성 유전단백체 학회 2011 Molecular & cellular toxicology Vol.7 No.4

The mammalian homolog of yeast Sir2protein is the sirtuin family of histone deacetylases (HDACs), a NAD+-dependent protein deacetylase in humans. Accumulating evidence suggests that sirtuin 2 (SIRT2) co-localizes with the microtubule network and deacetylates α-tubulin, and is involved in various cellular processes including calorie restriction-dependent life span extension, mitotic cell cycle regulation,cellular apoptosis, DNA damage repair, and genomic silencing. However, the underlying mechanisms of action remains poorly understood, especially in hepatocarcinogenesis. Hence in this study, to determine the association between the aberrant expression of SIRT2and liver cancer development and progression, SIRT2expression was investigated in ten selected hepatocellular carcinoma (HCC) tissues and matched normal liver tissues, using RT-PCR and Western blot analysis. Next, SIRT2 was disrupted by siRNA-mediated protein knockdown method to investigate the biological role of SIRT2 in hepatocarcinogenesis in Hep3B cells. As a result, we identified that SIRT2 expression was significantly up-regulated in HCC tissues compared to corresponding normal liver tissues. In addition, suppression of SIRT2 caused regression of tumor cell growth and proliferation. We also found that SIRT2could interact with α-tubulin and regulates the acetylation status of α-tubulin in Hep3B cells. In conclusion,we suggest that SIRT2 is aberrantly regulated in HCCs which may contribute to the mitogenic potential of tumor cells during the development and progression of HCC, and could be a novel molecular target for therapeutic intervention in liver cancer.

Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells

( Hong Xie ),( Jia Hu ),( Huan Pan ),( Ya Xin Lou ),( Ping Lv ),( Ying Yu Chen ) 생화학분자생물학회 2014 BMB Reports Vol.47 No.2

FAM176A (family with sequence similarity 176 member A) is a novel molecule related to programmed cell death. A decreased expression of FAM176A has been found in several types of human tumors in including lung cancers. In the present study, we investigated the biological activities of FAM176A on the human non-small cell lung cancer cell line H1299 cells. We constructed a recombinant adenovirus 5-FAM176A vector (Ad5-FAM176A) and evaluated the expression and anti-tumor activities in vitro. Cell viability analysis revealed that the adenovirus-mediated increase of FAM176A inhibited the growth of the tumor cells in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy and apoptosis that involved caspase activation. In addition, cell cycle analysis suggested that Ad5-FAM176A could induce cell cycle arrest at the G2/M phase, all of which suggested that adenovirus-mediated FAM176A gene transfer might present a new therapeutic approach for lung cancer treatment. [BMB Reports 2014; 47(2): 104-109]

Parameter Identification of Surrounding Rock in Underground Engineering Based on Complex Function Theory

Hong-Chuan Yan,Zhuo Li,Yong-Jian Shuai,Hong-Qiang Xie,Ming-Li Xiao,Ming-Guang Cai 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.6

To investigate the potential use of the complex function theory in displacement back analysis, a novel method for parameter identification is proposed by combining the complex function theory and the back-propagation neural network optimized by the particle swarm optimization algorithm. The finite element method is replaced with the complex function method to establish a nonlinear relationship between parameters and deformations of the surrounding rock around a complex underground cavern. As demonstrated by a virtual example of an arched tunnel, the deformation parameters were identified by the proposed method and demonstrated to be approximately equal to the predefined parameters. Subsequently, the proposed method is further applied to the inverse analysis of mechanical parameters for the surrounding rock in the underground powerhouse of the Baihetan Hydropower Station. The computed displacements based on the back-analyzed parameters show excellent agreement with the monitoring displacements. The average error between them is about 4.17%, so the proposed method provides the potential to viably enhance the back-analysis technique.

IN SITU PREPARATION AND INHIBITORY ACTIVITY OF HYDROXYAPATITE/SILVER NANOCOMPOSITE

HONG-ZHEN XIE,JIAN-DONG WANG,CHONG-XIAO LUO,JIN-KU LIU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.6

The hydroxyapatite (HAP) assembled microsphere chains/silver (Ag) nanocomposites were prepared by a facile in situ preparation method under hydrothermal condition. The crystal structure of products was characterized by X-ray di®raction (XRD) and the morphologies of products were investigated by transmission electron microscopy (TEM). The nanocomposites own large surface area and can hydrogen-bond to other substances by the hydroxyl group on the surface. The nanocomposites have good structural stability under the electrostatic e®ect of the silver nanoparticles and strong adsorbability of the HAP microsphere chains. The two substances combine to form special nanocomposite spheres. Specially, the HAP/Ag nanocomposites have high inhibitory activity and can be applied in the environment and medicine ¯elds. The nanocomposite structures can save the consumption of Ag materials.

Identification of a Methyltransferase Encoded by Gene ste16 and Its Function in Ebosin Biosynthesis of Streptomyces sp. 139

Hong-Guan Xie,Yong-Gang Bao,Li-ping Bai,Jun-Jie Shan,Rong Jiang,Yang Zhang,Lian-Hong Guo,Ren Zhang,Yuan Li 한국미생물학회 2009 The journal of microbiology Vol.47 No.2

Streptomyces sp. 139 generates a novel exopolysaccharide (EPS) designated as Ebosin, which exerts an antagonistic effect on IL-1R in vitro and anti-rheumatic arthritis activity in vivo. A ste gene cluster for Ebosin biosynthesis consisting of 27 ORFs was previously identified in our laboratory. In this paper, ste16 was expressed in Escherichia coli BL21 and the recombinant protein was purified, which has the ability to catalyze the transfer of the methyl group from S-adenosylmethionine (AdoMet) to dTDP-4-keto-6-deoxy-D-glucos, which was thus identified as a methyltransferase. In order to determine the function of ste16 in Ebosin biosynthesis, the gene was disrupted with a double crossover via homologous recombination. The monosaccharide composition of EPS-m generated by the mutant strain Streptomyces sp. 139 (ste16-) was found to differ from that of Ebosin. The IL-1R antagonist activity of EPS-m was markedly lower than that of Ebosin. These experimental results have shown that the ste16 gene codes for a methyltransferase which is involved in Ebosin biosynthesis.

Inhibition of Breast Cancer Metastasis Via PITPNM3 by Pachymic Acid

Hong, Ri,Shen, Min-He,Xie, Xiao-Hong,Ruan, Shan-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Breast cancer metastasis is the most common cause of cancer-related death in women. Thus, seeking targets of breast tumor cells is an attractive goal towards improving clinical treatment. The present study showed that CCL18 from tumor-associated macrophages could promote breast cancer metastasis via PITPNM3. In addition, we found that pachymic acid (PA) could dose-dependently inhibit migration and invasion of MDA-MB-231cells, with or without rCCL18 stimulation. Furthermore, evidence was obtained that PA could suppress the phosphorylation of PITPNM3 and the combination of CCL18 and PITPNM3. Therefore, we speculate that PA could inhibit breast cancer metastasis via PITPNM3.

Patent Ductus Arteriosus and Pulmonary Valve Stenosis in A Patient with 18p Deletion Syndrome

Chun-Hong Xie,Jian-Bin Yang,Fang-Qi Gong,Zheng-Yan Zhao 연세대학교의과대학 2008 Yonsei medical journal Vol.49 No.3

We report on a patient with a partial deletion on the short arm of chromosome 18 (del 18p), who presented with dysmorphic features and delayed developmental milestones as well as with a patent ductus arteriosus (PDA) and pulmonary valve stenosis (PS). Several forms of congenital heart disease (CHD) are found in about 10% of patients with del (18p), but coexisting PDA and PS have not been reported. Del (18p) must be considered in patients with characteristic phenotypic abnormalities and congenital heart disease, including a combination of PDA and PS.