http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Ding Xin Wu ),( Lin Chun Wang ),( Yuwei Li ),( Shu Miao Zhao ),( Nan Peng ),( Yun Xiang Liang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.2
An exo-β-D-glucosaminidase (AorCsxA) from Aspergillus oryzae FL402 was heterologously expressed and purified. The deduced amino acid sequence indicated that AorCsxA belonged to glycoside hydrolase family 2. AorCsxA digested colloid chitosan into glucosamine but not into chitosan oligosaccharides, demonstrating exo-β-D-glucosaminidase (CsxA) activity. AorCsxA exhibited optimal activity at pH 5.5 and 50°C; however, the enzyme expressed Pichia pastoris (PpAorCsxA) showed much stronger thermostability at 50°C than that expressed in Escherichia coli (EcAorCsxA), which may be related to glycosylation. AorCsxA activity was inhibited by EDTA and most of the tested metal ions. A single amino acid mutation (F769W) AorCsxA significantly enhanced the specific activity and hydrolysis velocity as revealed comparison of V_{max} and k_{cat} values with those of the wild-type enzyme. The three-dimensional structure suggested the tightened pocket at the active site of F769W enabled efficient substrate binding. The AorCsxA gene was heterologously expressed in P. pastoris, and one transformant was found to produce 222 U/ml activity during the high-cell-density fermentation. This AorCsxA-overexpressing P. pastoris strain is feasible for large-scale production of AorCsxA.
Ding, Jing,Tang, Jie,Chen, Xin,Men, Hai-Tao,Luo, Wu-Xia,Du, Yang,Ge, Jun,Li, Cong,Chen, Ye,Cheng, Ke,Qiu, Meng,Liu, Ji-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10
Background: Preexisting type 2 diabetes mellitus (T2DM) affects the prognosis and mortality of patients with some cancers. Insulin like growth factor (IGF) and insulin receptor (IR) signaling axes play important roles in both cancer and diabetes development. We aimed to explore the expression characteristics of proteins in IGF/IR axis in non-small cell lung cancer (NSCLC) cases with preexisting T2DM. Methods: Fifty-five NSCLC patients with preexisting T2DM were retrospectively included and matched by 55 NSCLC without diabetes at a 1:1 ratio. The expression of proteins in IGF/IR axis was detected by immunohistochemical staining. Clinicopathological data were collected to analyze their relationship with the protein expression. Results: Both IGF 1 receptor (IGF-1R) and insulin receptor substrate 2 (IRS-2) showed higher expression in the NSCLC with T2DM group, compared with those without T2DM. The high expression of IGF-1R and IRS-2 were found to be negatively associated with lymph node metastases and T staging in the T2DM group, respectively, and IRS-2 expression was also found more in the subgroup whose T2DM duration was more than 4 years. No difference was detected in the expression of IRS-1, IGF-1, IGF-2, IGFBP3, IR and mTOR between groups with or without T2DM. Conclusion: Our study found higher expression of IGF-1R and IRS-2 proteins in NSCLC patients with preexisting T2DM, and that there was an association with early stage NSCLC, which suggested that IGF signaling may play an important early event in development of NSCLC associated with diabetes.
Huizhi Wu,Qian Geng,Yonghan Li,Yuqian Song,Jiaqi Chu,Rong Zhou,Xin Ning,Senjie Dong,Ding Yuan 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-
Here, a multifunctional poly (lactic acid)/copper-based metal–organic framework (PLA/CuMOF) degradablecomposite membrane featuring superior antibacterial and self-cleaning properties was fabricated viaa simple electrospinning process for high- efficiency filtration/separation. Benefiting from the decrease offiber diameter, the improved surface roughness and the surface charge of CuMOF, PLA/CuMOF fibrousmembrane achieved excellent capture ability for ultra-fine particles and superb purification capabilityfor real PM2.5 smoke. The differences of filtration capacity between PLA membrane and PLA/CuMOF membranewas further explored using analogue simulation with dynamic particle capture and airflow fielddistribution. Impressively, PLA/CuMOF fibrous membrane combines robust self-cleaning ability, effectiveantibacterial effect, and thermal management capability. Moreover, owing to the special selective wettabilityand chemical stability, PLA/CuMOF membrane possessed the stable oil–water separation performanceunder harsh environment (e.g., high acid, alkali, and salt). This degradable multifunctionalfiltration/separation fibrous membrane emerges a broad application prospect ranging from environmentalgovernance, industrial security to personal protection.
Liu, Jin-Xin,Luo, Rong-Cheng,Li, Rong,Li, Xia,Guo, Yu-Wu,Ding, Da-Peng,Chen, Yi-Zhi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15
This meta-analysis was conducted to examine whether the genotype status of Val158Met polymorphism in catechol-O-methyltransferase (COMT) is associated with endometrial and ovarian cancer risk. Eligible studies were identified by searching several databases for relevant reports published before January 1, 2014. Pooled odds ratios (ORs) were appropriately derived from fixed-effects or random-effects models. In total, 15 studies (1,293 cases and 2,647 controls for ovarian cancer and 2,174 cases and 2,699 controls for endometrial cancer) were included in the present meta-analysis. When all studies were pooled into the meta-analysis, there was no evidence for significant association between COMT Val158Met polymorphism and ovarian cancer risk (Val/Met versus Val/Val: OR=0.91, 95% CI=0.76-1.08; Met/Met versus Val/Val: OR=0.90, 95% CI=0.73-1.10; dominant model: OR=0.90, 95% CI=0.77-1.06; recessive model: OR=0.95, 95% CI=0.80-1.13). Similarly, no associations were found in all comparisons for endometrial cancer (Val/Met versus Val/Val: OR 0.97, 95% CI=0.77-1.21; Met/Met versus Val/Val: OR=1.02, 95% CI=0.73-1.42; dominant model: OR=0.98, 95% CI=0.77-1.25; recessive model: OR=1.02, 95% CI=0.87-1.20). In the subgroup analyses by source of control and ethnicity, no significant associations were found in any subgroup of population. This meta-analysis strongly suggests that COMT Val158Met polymorphism is not associated with increased endometrial and ovarian cancer risk.
Surface Modification with Pluronic P123 Enhances Transfection Efficiency of PAMAM Dendrimer
Xiang Wang,Shen Gao,Xin Wu,Wei Fan,Baoyue Ding,Xiaoyu Wang,Wei Zhang,Xueying Ding,Jing Gao,Quangang Zhu,Jiyong Liu,Zhen Cai 한국고분자학회 2012 Macromolecular Research Vol.20 No.2
To improve gene delivery efficiency and decrease cytotoxicity of polyamidoamine (PAMAM) polymers,P123-g-PAMAM was synthesized by modifying PAMAM with pluronic P123. The structure of the synthesized polymers was analyzed using proton nuclear magnetic resonance. The polymers were able to self-assemble with DNA,forming nanometer-scale complexes. Particle size measurement confirmed that the mean diameter of the polyplexes was 100-250 nm. The cytotoxicity and transfer efficiency were measured and compared with those of PEI and PAMAM. All of the polyplexes showed significantly low cytotoxicity in the MCF-7, HepG2, and 293T cell lines. In addition, the low level of P123 grafting to PAMAM showed significantly higher transfection efficiency than unmodified PAMAM at the optimal N/P ratio. These results suggest that P123-ylated PAMAM may prove as a useful carrier for gene delivery.
Compatibility of Neural Stem Cells with Functionalized Selfassembling Peptide Scaffold In vitro
Zhen Xing Zhang,Ding Jun Hao,Qi Xin Zheng,Yong Chao Wu 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.4
In this study, we evaluated the behavior of neural stem cells (NSCs) using a new peptide hydrogel scaffold named IKVAVmx, which was made by mixing self-assembling peptide RADA16 and designer peptide RADA16-IKVAV solutions. NSCs derived from rat cerebral cortex were culture-expanded in neuorobasal medium and seeded on the RADA16 and IKVAVmx hydrogels. Cells could penetrate the hydrogels and form a 3D cellular network. Compared to pure RADA16 scaffold, we found that IKVAVmx scaffold significantly promoted cell proliferation and stimulated cell migration into the 3D scaffold. Moreover, Immunocytochemistry and Western blot analysis indicated that the differentiation ratio of neurons from NSCs in IKVAVmx scaffold was higher than that in pure RADA16 scaffold. These results suggested that this new hydrogel scaffold provided an ideal substrate for NSCs 3D culture and suggested its further application for neural tissue engineering.
Pan, Lei,Han, Li-Li,Tao, Li-Xin,Zhou, Tao,Li, Xia,Gao, Qi,Wu, Li-Juan,Luo, Yan-Xia,Ding, Hui,Guo, Xiu-Hua Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9
Objectives: Although there are many reports about the risk of breast cancer, few have reported clinical factors including history of breast-related or other diseases that affect the prevalence of breast cancer. This study explores these risk factors for breast cancer cases reported in Beijing in 2009. Materials and Methods: Data were derived from a Beijing breast cancer screening performed in 2009, of 568,000 women, from 16 districts of Beijing, all aged between 40 and 60 years. In this study, multilevel statistical modeling was used to identify clinical factors that affect the prevalence of breast cancer and to provide more reliable evidence for clinical diagnostics by using screening data. Results and Conclusion: Those women who had organ transplants, compared with those with none, were associated with breast cancer with an odds ratio (OR)=65.352 [95% confidence interval (CI): 8.488-503.165] and those with solid breast mass compared with none had OR=1.384 (95% CI: 1.022-1.873). Malignant tendency was strongly associated with increased risk of breast cancer, OR=207.999(95% CI: 151.950-284.721). The risk of breast cancer increased with age, $OR_1$=2.759 (95% CI: 1.837-4.144, 56-60 vs. 40-45), $OR_2$=2.047 (95% CI: 1.394-3.077, 51-55 vs. 40-45), $OR_3$=1.668 (95% CI: 1.145-2.431). Normal results of B ultrasonic examination show a lower risk among participants, OR= 0.136 (95% CI: 0.085-0.218). Those women with ductal papilloma compared with none were associated with breast cancer, OR=6.524 (95% CI: 1.871-22.746). Therefore, this study suggests that clinical doctors should pay attention to these high-risk factors.