우수방사성의약품 생산시설 개발

신병철,정원명,박상현,이규일,박경배,박진호 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

Manufacturing facilities of the pharmaceuticals must meet certain level of the cleanness required so that foreign substances such as dust, moisture, heat, microorganism, or virus do not contaminate the product. In case of radiopharmaceuticals for medical treatment and diagnosis, not only should the operators and environment be protected from radiation but also need to be isolated from the foreign contaminant. Therefore, manufacturing facilities for radiopharmaceuticals must satisfy the design standards of both hot cell and clean room which are specified by GNP. However, standards of maintaining negative pressure for preventing spread of radioactive contaminant in isolated facilities conflict with the standards of maintaining positive pressure for keeping cleanness. To solve this problem, are pressure of hot cell was designed lower than in the adjacent area to meet standards of the radiation safety. To keep higher cleanness in certain part of the hot cell for filling, minimal relative positive pressure allows. In order to effectively maintain the cleanness that is required for production of Tc-99m generator, which takes 70% of whole demand of radiopharmaceuticals, the rooms placed in each side of production room are used as a buffer area and three lead hot cells are installed in production room. In this research, we established the appropriate engineered design concept for Tc-99m generator manufacturing facility, which satisfies both GMP cleanness standard for preventing particles, bacteria, other contaminants and the regulations of radiation safety for supervising and controlling the amount of radiation exposure and exhausted radioactivity. And the concept of multi-buffer zones is introduced to apply negative air pressure for hot cell with first priority and to continue relative positive air pressure for clean room.

A Study of Psychotherapy Plan through Game Graphics Focused on Color Preference of Children

Choung-Hun Lee,Su-Hyun Jin,Won-Hyung Lee 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.7

Colors are recognized by human vision or visual organ. In particular, many studies show that these colors have been proved to give great influences on cognitive development and emotional formation of children. The subconsciousness of children is detectable by colors and through this, their inner emotions and subconsciousness can be revealed. Children have higher interaction of emotion and feelings with colors than adults. Moreover, children tend to express their subconsciousness through colors. However, children are defenselessly exposed to colors coming from the IT devices due to the broad diffusion of these appliances. Different types of media materials give impact on the emotions and cognitive development of children. Likewise, the colors represented by media materials considerably exert influence on the emotions and cognitive development of children. With this in mind, this study proposes design game graphics through analysis of colors, composition, nature and character of these colors preferred by children.

Treatment outcome of postoperative radiotherapy for retroperitoneal sarcoma

Hyun Jin Lee,Si Yeol Song,Tae-Won Kwon,Jeong Hwan Yook,Song-Cheol Kim,Duck-Jong Han,Choung-Soo Kim,Hanjong Ahn,Heung Moon Chang,Jin-Hee Ahn,Eun Jin Jwa,Sang-Wook Lee,Jong Hoon Kim,Eun Kyung Choi,Seong 대한방사선종양학회 2011 Radiation Oncology Journal Vol.29 No.4

Purpose: To evaluate the treatment outcome and prognostic factor after postoperative radiotherapy in retroperitoneal sarcoma. Materials and Methods: Forty patients were treated with surgical resection and postoperative radiotherapy for retroperitoneal sarcoma from August 1990 to August 2008. Treatment volume was judged by the location of initial tumor and surgical field, and 45-50 Gy of radiation was basically delivered and additional dose was considered to the high-risk area. Results: The median follow-up period was 41.4 months (range, 3.9 to 140.6 months). The 5-year overall survival (OS) was 51.8% and disease free survival was 31.5%. The 5-year locoregional recurrence free survival was 61.9% and distant metastasis free survival was 50.6%. In univariate analysis, histologic type (p = 0.006) was the strongest prognostic factor for the OS and histologic grade (p = 0.044) or resection margin (p = 0.032) had also effect on the OS. Histologic type (p = 0.004) was unique significant prognostic factor for the actuarial local control. Conclusion: Retroperitoneal sarcoma still remains as a poor prognostic disease despite the combined modality treatment including surgery and postoperative radiotherapy. Selective dose-escalation of radiotherapy or combination of effective chemotherapeutic agent must be considered to improve the treatment result especially for the histopathologic type showing poor prognosis.

Anti-inflammatory and anti-painful effects of Ostericum korearnum Maximowicz against lipopolysaccharides induced inflammation .

KWON Yong Soo CHOUNG Se Young,LEE Cheol-Koo,LEE Kwang Won,HONG Jong Ki,SHIN Seung won,HYUN Sun Hee 대한약학회 2006 大韓藥學會 總會 및 學術大會 Vol.2006 No.2

Suppression of Inflammation by Recombinant <i>Salmonella typhimurium</i> Harboring <i>CCL22</i> MicroRNA

Yoon, Won Suck,Ryu, Seung Rel,Lee, Seung Seok,Chae, Yang Seok,Kim, Eun Jae,Choi, Ji Hyun,Oh, Sejin,Park, Se Ho,Choung, Ji Tae,Yoo, Young,Park, Yong Keun Mary Ann Liebert, Inc 2012 DNA and Cell Biology Vol. No.

인회석 박막 피복 도관과 Brain-derived neurotrophic factor(BDNF) 유전자 이입 슈반세포를 이용한 백서 좌골신경 재생에 관한 연구

최원재(Won-Jae Choi),안강민(Kang-Min Ahn),황순정(Soon-Jeong Hwang),정필훈(Pill-Hoon Choung),김명진(Myung-Jin Kim),김남열(Nam-Yeol Kim),유상배(Sang-Bae Yoo),장정원(Jeong-Won Jahng),김현만(Hyun-Man Kim),김중수(Joong-Soo Kim),김윤희(Yun 대한구강악안면외과학회 2005 대한구강악안면외과학회지 Vol.31 No.3

Purpose of Study: Peripheral nerve regeneration depends on neurotrophism of distal nerve stump, recovery potential of neuron, supporting cell like Schwann cell and neurotrophic factors such as BDNF. Peripheral nerve regeneration can be enhanced by the conduit which connects the both sides of transected nerve. The conduit maintains the effects of neurotrophism and BDNF produced by Schwann cells which can be made by gene therapy. In this study, we tried to enhance the peripheral nerve regeneration by using calcium phosphate coated porous conduit and BDNF-Adenovirus infected Schwann cells in sciatic nerve of rats. Materials and Methods: Microporous filter which permits the tissue fluid essential for nerve regeneration and does not permit infiltration of fibroblasts, was made into 2mm diameter and 17mm length conduit. Then it was coated with calcium phosphate to improve the Schwann cell adhesion and survival. The coated filter was evaluated by SEM examination and MTT assay. For effective allogenic Schwann cell culture, dorsal root ganglia of 1-day old rat were extracted and treated with enzyme and antimitotic Ara-C. Human BDNF cDNA was obtained from cDNA library and amplified using PCR. BDNF gene was inserted into adenovirus shuttle vector pAACCMVpARS in which E1 was deleted. We infected the BDNF-Ad into 293 human mammary kidney cell-line and obtained the virus plaque 2 days later. RT-PCR was performed to evaluate the secretion of BDNF in infected Schwann cells. To determine the most optimal m.o.i of BDNF-Ad, we infected the Schwann cells with LacZ adenovirus in 1, 20, 50, 75, 100, 250 m.o.i for 2 hours and stained with 􀝜-galactosidase. Rats(n=24) weighing around 300g were used. Total 14mm sciatic nerve defect was made and connected with calcium phosphate coated conduits. Schwann cells(1x106) or BDNF-Ad infected Schwann cells(1x106) were injected in conduit and only media(MEM) was injected in control group. Twelve weeks after surgery, degree of nerve regeneration was evaluated with gait analysis, electrophysiologic measurements and histomorphometric analysis. Results: 1. Microporous Millipore filter was effective conduit which permitted the adhesion of Schwann cells and inhibited the adhesion of fibroblast. We could enhance the Schwann cell adhesion and survival by coating Millipore filter with calcium phosphate. 2. Schwann cell culture technique using repeated treatment of Ara-C and GDNF was established. The mean number of Schwann cells obtained 1 and 2 weeks after the culture were 1.54±4.0×106 and 9.66±9.6×106. 3. The mRNA of BDNF in BDNF-Ad infected Schwann cells was detected using RT-PCR. In Schwann cell 0.69 μg/μl of DNA was detected and in BDNF-Adenovirus transfected Schwann cell 0.795 μg/μl of DNA was detected. The most effective infection concentration was determined by LacZ Adenovirus and 75 m.o.i was found the most optimal. Conclusion: BDNF-Ad transfected Schwann cells successfully regenerated the 14mm nerve gap which was connected with calcium phosphate coated Millipore filter. The BDNF-Ad group showed better results compared with Schwann cells only group and control group in aspect to sciatic function index, electrophysiologic measurements and histomorphometric analysis.

Treatment outcome of postoperative radiotherapy for retroperitoneal sarcoma

Lee, Hyun-Jin,Song, Si-Yeol,Kwon, Tae-Won,Yook, Jeong-Hwan,Kim, Song-Cheol,Han, Duck-Jong,Kim, Choung-Soo,Ahn, Han-Jong,Chang, Heung-Moon,Ahn, Jin-Hee,Jwa, Eun-Jin,Lee, Sang-Wook,Kim, Jong-Hoon,Choi, The Korean Society for Radiation Oncology 2011 Radiation Oncology Journal Vol.29 No.4

Purpose: To evaluate the treatment outcome and prognostic factor after postoperative radiotherapy in retroperitoneal sarcoma. Materials and Methods: Forty patients were treated with surgical resection and postoperative radiotherapy for retroperitoneal sarcoma from August 1990 to August 2008. Treatment volume was judged by the location of initial tumor and surgical field, and 45-50 Gy of radiation was basically delivered and additional dose was considered to the high-risk area. Results: The median follow-up period was 41.4 months (range, 3.9 to 140.6 months). The 5-year overall survival (OS) was 51.8% and disease free survival was 31.5%. The 5-year locoregional recurrence free survival was 61.9% and distant metastasis free survival was 50.6%. In univariate analysis, histologic type (p = 0.006) was the strongest prognostic factor for the OS and histologic grade (p = 0.044) or resection margin (p = 0.032) had also effect on the OS. Histologic type (p = 0.004) was unique significant prognostic factor for the actuarial local control. Conclusion: Retroperitoneal sarcoma still remains as a poor prognostic disease despite the combined modality treatment including surgery and postoperative radiotherapy. Selective dose-escalation of radiotherapy or combination of effective chemotherapeutic agent must be considered to improve the treatment result especially for the histopathologic type showing poor prognosls.

VHDL을 구현하기 위한 중간코드 설계

김충석(Choung-seok Kim),김현배(Hyun-bae Kim),윤유헌(Yoo-Hun Won) 한국정보과학회 1990 한국정보과학회 학술발표논문집 Vol.17 No.1

본 연구는 하드웨어 설계자동화 분야에서 요구되고 있는 HDL 언어 중 IEEE에서 표준화한 VHDL 언어를 사용하기 위한 지원 환경 중에서 VHDL source program을 입력으로 받아 중간코드를 생성하는 Analyzer 시스팀을 구축하기 위한 중간코드에 관한 연구이다. 각 tool들 사이에 공유되어 사용될 중간코드의 설계를 위해 VHDL 언어의 formal definition에 따라 IDL 언어로 기술하여 중간코드를 설계한다. 연구의 내용은 중간코드의 배경과 개념, 설계 방법 및 절차를 기술하고 현재 사용중인 중간코드의 종류를 분석하고 있으며, VHDL 언어를 사용하여 중간코드를 생성하여 보았다. 이러한 VHDL 중간 코드는 내부적으로는 graph 형태를 띠고 있으며 외부적으로는 사용자가 인식할 수 있는 텍스트 형태의 파일로 나타낸다.

어깨관절의 수동적 내회전 관절 가동범위의 측정 방법에 따른 신뢰도와 상완골두의 전방 활주 거리 비교

김현숙(Hyun-sook Kim),이원휘(Won-hwee Lee),정성대(Sung-dae Choung) 한국전문물리치료학회 2010 한국전문물리치료학회지 Vol.17 No.3

The purpose of this study was to measure intra-rater and inter-rater reliability and range of motion for measurement of passive shoulder internal rotation range of motion and to compare anterior glide dis tance of humeral head in three methods. Fifty healthy subjects and fifty patients with shoulder muscu loskeletal pain were recruited for this study. The subjects' passive shoulder internal rotation range of motion was measured by visual estimation, manual stabilization, and pressure biofeedback unit methods In two trials, measurements were perfonned on each subject by two examiners. Intraclass correlation co efficient (ICC(3,1)) was used to detennine the reliabiliη of each measurement. The intra-rater reliability of the three methods was excellent (ICC=.77~.93) in both groups. The inter-rater reliability of the 、risual estimation method was poor (ICC=.20, .29), the manual scapular stabilization method was poor and fair (ICC=.09, .50), and the pressure biofeedback unit method was excellent (ICC .86, .75) in the experimental and control groups. In the expenmental group, the difference of examined range of motion by each examiner was significant in the 、risual estimation method and manual scapular stabilization method, but there was an insignificant difference between the groups is the pressure biofeedback unit method. This result suggests that the intra-rater and inter-rater reliabiliη of a pressure biofeedback unit was better than the other methods. The difference in distance of the anterior glide of humeral head was insignificant among all the methods. The pressure biofeedback unit method was the most reliable method, so it is proposed to be a new and reliable method to measure internal rotation range of motion.

The oncoprotein, gankyrin, is up-regulated in middle ear cholesteatoma

Kim, Ki Hyun,Lim, Hye Jin,Kim, Yeon Ju,Kim, Seung Won,Kim, Young Sun,Tian, Chunjie,Park, Keehyun,Park, Tae Jun,Choung, Yun-Hoon Scandinavian University Press 2014 Acta oto-laryngologica Vol.134 No.3

<P><I>Conclusion:</I> Gankyrin seems to be a better biomarker for cholesteatoma compared with Ki-67. <I>Objective:</I> Gankyrin is an oncoprotein, and occurs in cancers but not in benign diseases. The goal of this study was to compare expression of gankyrin, p53, and a proliferation marker (Ki-67) in cholesteatoma and retroauricular skin (RAS), and to evaluate their significance as clinical parameters. <I>Methods:</I> The levels of expression of gankyrin, Ki-67, and p53 in 10 cholesteatoma and 10 paired samples of normal RAS were evaluated by immunohistochemical staining and Western blot. The results were compared with clinical profiles to investigate a correlation. <I>Results:</I> The expression of gankyrin, Ki-67, and p53 proteins was observed in both basal and suprabasal layers of cholesteatoma. The intensity of gankyrin expression was ‘positive’ in two cases (20%) and ‘strongly positive’ in eight cases (80%); p53 expression in the suprabasal layer was ‘positive’ in 70% of cases; and the Ki-67 staining was ‘focal’ in 80% of cases. In RAS, these proteins were expressed dominantly in the basal layer. Western blot analysis showed that the gankyrin band was more intense in cholesteatoma than in RAS for three of four cases (<I>p</I> < 0.05). However, there was no significant difference in the expression of gankyrin, Ki-67, and p53 according to clinical variables.</P>