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( Boo Sung Kim ),( Youn Zoo Cho ),( Soon Koo Baik ),( Moon Young Kim ),( Won Ki Hong ),( Hye Won Hwang ),( Jin Hyung Lee ),( Myeong Hun Chae ),( Seung Yong Shin ),( Jung Min Kim ),( Mee Yon Cho ),( Sa 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Cirrhosis is a long-term consequence of chronic hepatic injury with fibrosis and no effective therapy except liver transplantation is currently available for decompensated cirrhosis. However, some practical limitations in liver transplantation lead us to a need for new therapeutic paradigm in this field. Recent reports have shown that the mesenchymal stem cells (MSCs) have the plasticity to differentiate into some kinds of tissue cells and improve organ function. Hence, we investigated the effect of direct inoculation of human bone marrow derived MSCs (BM-MSCs) in thioacetamide (TAA)-induced cirrhosis in a rat model. Methods: Adult Sprague-Dawley rats were allocated into three groups (each group, n = 15) as follows: G1, shame; G2, TAAcontrol; G3, TAA+BM-MSC. To induce cirrhosis, 200mg/kg TAA injection was done twice a week for 12weeks in G2 and G3. 2×106 cells of amplified human BM-MSCs were injected directly into the right liver lobe twice, at weeks 6 and 8 in G3. At 12 weeks, the effect of BM-MSCs on cirrhosis was analyzed histomorphologically using Laennec scores. α-Smooth muscle actin(α-SMA) expression by immunohistochemical staining, relative expression of collagen type 1, and transforming growth factor β (TGF-β) were also evaluated by real-time reverse transcriptase- polymerase chain reaction. Results: Laennec scores were 0, 5.4±0.7 and 3.7±1.06 in G1, G2 and G3, respectively. Histologically, BM-MSCs injected group (G3) showed significant suppression of hepatic fibrosis compared with TAA-control group (G2)(P<0.001). Expressions of α-SMA(%) were significantly lower in G3 than in G2 (3.08±1.26 vs. 7.00±4.12, P<0.05). Also, the relative expression of collagen type 1 and TGF-β1 in RT-PCR were 0.64±0.24, 2.06±0.51, 1.32±0.31 and 0.62±0.28, 5.89±3.05, 2.22±1.41 in G1, G2 and G3, respectively P<0.005). Conclusions: Our results showed that BM-MSCs could attenuate liver fibrosis in rats with TAA-induced cirrhosis, raising the possibility for clinical use of BM-MSCs in the treatment of cirrhosis.
이석구,부성민 忠南大學校 環境問題硏究所 1999 環境硏究 Vol.17 No.-
Daily vertical distribution of phytoplankton was investigated during some blue-green algal blooming in Napo, Kumgang river in September, 1999. The surface water temperature was in a range of 28.8℃ (at 16:00) - 27.3℃ (noon). Although there few changes in the concentration of nitrite (0.062±0.002 - 0.064±0.001 mgN/ℓ) and nitrate (1.770±0.046 - 1.823±0.075 mgN/ℓ), ammonium ranged from 0.230±0.042 mgN/ℓ to 0.180±0.046 mgN/ℓ. Phosphate concentration ranged from 1.227±1.685 mgP/ℓ to 0.530±0.691 mgP/ℓ. Based on cell counting, the Bacillariophyceae and Chlorophyceae moved up and down along water column markedly. This result indicates that daily vertical distribution of planktonic green algae and diatoms may be determined by light rather than nutrients. The Cyanophyceae showed the highest standing crops and almost aggregated to the upper regions of water column throughout day. Of the blue-green algae, Microcystis species were very abundant in every sampling. It is probable that the cells of Microcystis species are covered with mucilage and readily floating compared to other filamentous species.