A New ent-Kaurane type Diterpenoid Glycoside from Inula japonica Thunb

Jiang Jiang Qin,Jia Xian Zhu,Wei Dong Zhang1,2,Yan Zhu,Jian Jun Fu,Xiao Hua Liu,Hui Zi Jin 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.10

A new ent-kaurane type diterpenoid glycoside, 17-O-β-D-glucopyranosyl-16α-ent-kauran-19-oic acid (1), together with 17-hydroxy-16α-ent-kauran-19-oic acid (2), 16α,17-dihydroxyl-ent-kauran-19-oic acid (3), and 16α-hydroxy-17-acetoxy-ent- kauran-19-oic acid (4) were isolated from the aerial parts of Inula japonica Thunb. The structure of 1 was determined mainly by use of 1D and 2D NMR spectroscopic techniques including HSQC, 1H-1H COSY, HMBC, and NOESY. In addition, 4 exhibited significant inhibitory activity on NO production in LPS-stimulated RAW264.7 cells with IC50 value of 14.3 μg/mL.

Silencing of long noncoding RNA PVT1 inhibits podocyte damage and apoptosis in diabetic nephropathy by upregulating FOXA1

Dong-Wei Liu,Jia-Hui Zhang,Feng-Xun Liu,Xu-Tong Wang,Shao-Kang Pan,Deng-Ke Jiang,Zi-Hao Zhao,Zhang-Suo Liu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The number of patients with diabetic nephropathy (DN) is still on the rise worldwide, and this requires the development of new therapeutic strategies. Recent reports have highlighted genetic factors in the treatment of DN. Herein, we aimed to study the roles of long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) and histone 3 lysine 27 trimethylation (H3K27me3) in DN. A model of DN was established by inducing diabetes in mice with streptozotocin. Mouse podocyte clone 5 (MPC5) podocytes and primary podocytes were cultured in normal and high glucose media to observe cell morphology and to quantify PVT1 expression. The roles of PVT1 and enhancer of zeste homolog 2 (EZH2) were validated via loss-of-function and gain-of-function in vitro experiments to identify the interactions among PVT1, EZH2, and forkhead box A1 (FOXA1). The podocyte damage and apoptosis due to PVT1 and FOXA1 were verified with in vivo experiments. PVT1 was highly expressed in MPC5 and primary podocytes in DN patients and in cultures grown in high glucose medium. A large number of CpG (C-phosphate-G) island sites were predicted at the FOXA1 promoter region, where PVT1 recruited EZH2 to promote the recruitment of H3K27me3. The silencing of PVT1 or the overexpression of FOXA1 relieved the damage and inhibited the apoptosis of podocytes in DN, as was evidenced by the upregulated expression of synaptopodin and podocin, higher expression of Bcl-2, and lower expression of Bax and cleaved caspase-3. The key findings of this study collectively indicate that the suppression of lncRNA PVT1 exerts inhibitory effects on podocyte damage and apoptosis via FOXA1 in DN, which is of clinical significance.

Evaluation on Dry Sliding Wear Behavior of (TiB+TiC)/Ti- 6Al-4V Matrix Composite

Zi-Run Yang,Hai-Xiang Huan,Cui-Feng Jiang,Wen-Min Li,Xue-Ran Liu,류성기 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.8

The dry sliding wear tests were performed for 10 vol.% (TiB+TiC)/Ti-6Al-4V matrix composites at various test conditions. The morphology and phases of worn surfaces and cross-sectional surfaces were investigated by using scanning electron microscopy (SEM) and X-ray diffraction (XRD). The wear loss of composites with the increase of load, but the wear behavior got more complex when the sliding speed and ambient temperature got higher. Low wear loss was achieved at lower sliding speed with higher temperature or higher sliding speed with lower temperature. A tribo-layer was always formed on worn surface during testing. It possessed different features and thus presented different effects during sliding wear. Meanwhile, the TiB and TiC particulates strengthened the Ti-6Al- 4V matrix, which increased the thermal stability of composites and postponed the occurrence of severe plastic deformation. In the mild wear region, those particulates could directly resist wear by reducing adhesive wear. In the steady stage, fractured TiB and TiC particulates participated in forming the tribo-layer together with wear debris, which effectively hindered the plastic deformation and thermal softening of the matrix. Experiment results suggest that the existence of different characteristic tribo-layers would result in the variation of wear behavior.

Plasmonic Nanoantennae Fabricated by Focused Ion Beam Milling

Jiang-Tao Lv,Yuan Yan,Wei-Kang Zhang,Yun-Hui Liu,Zi-Yu Jiang,Guang-Yuan Si 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

We show a novel approach to fabricate plasmonic nanoantennae based on a maskless focused ion beam nanoring patterning process. Antenna nanoarrays with desired outlines are achieved by precisely controlling the geometric parameters during the milling process. Various nanoantenna designs of bow-tie, nanoclusters (pentamers), and ellipsoid shaped satellites surrounded particle lattices arerealized. The whole fabrication method is programmable and monolithic since only a one-step milling process is involved. The opticalproperties are experimentally characterized. Such nanoantennae may find extensive applications in chemical/bio-sensing due toremarkably enhanced near field intensity at the plasmon resonance.

Isolation of NH4+-Tolerant Mutants of Actinobacillus succinogenes for Succinic Acid Production by Continuous Selection

( Gui Zi Ye ),( Min Jiang ),( Jian Li ),( Ke Quan Chen ),( Yong Lan Xi ),( Shu Wen Liu ),( Ping Wei ),( Ping Kai Ouyang ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.8

Actinobacillus succinogenes, a representative succinic-acid-producing microorganism, is seriously inhibited by ammonium ions, thereby hampering the industrial use of A. succinogenes with ammonium-ion-based materials as the pH controller. Therefore, this study isolated an ammonium-ion-tolerant mutant of A. succinogenes using a continuous-culture technique in which all the environmental factors, besides the stress (ammonium ions), were kept constant. Instead of operating the mutant-generating system as a nutrient-limited chemostat, it was used as a nutrientunlimited system, allowing the cells to be continuously cultured at the maximum specific growth rate. The mutants were isolated on agar plates containing the acid-base indicator bromothymol blue and a high level of ammonium ions that would normally kill the parent strain by 100%. When cultured in anaerobic bottles with an ammonium ion concentration of 354 mmol/l, the mutant YZ0819 produced 40.21 g/l of succinic acid with a yield of 80.4%, whereas the parent strain NJ113 was unable to grow. When using NH4OH to buffer the culture pH in a 3.0 l stirred bioreactor, YZ0819 produced 35.15 g/l of succinic acid with a yield of 70.3%, which was 155% higher than that produced by NJ113. In addition, the morphology of YZ0819 changed in the fermentation broth, as the cells were aggregated from the beginning to the end of the fermentation. Therefore, these results indicate that YZ0819 can efficiently produce succinic acid when using NH4OH as the pH controller, and the formation of aggregates can be useful for transferring the cells from a cultivation medium for various industrial applications.

Ultrasound Utility for Predicting Biological Behavior of Invasive Ductal Breast Cancers

Zhang, Lei,Liu, Yu-Jie,Jiang, Shuang-Quan,Cui, Hao,Li, Zi-Yao,Tian, Jia-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Purpose: The aim of the study was to evaluate the correlation of ultrasound features with breast cancer molecular status. Materials and Methods: A retrospective review was performed of ultrasound findings in 263 patients diagnosed with breast invasive ductal carcinoma for comparison with immunohistochemistric results were obtained from each lesion. Relationships between ultrasound findings and molecular status were investigated by using multiple regression analysis by means of stepwise logistic regression. Differences in ultrasound criteria were assessed among women with different molecular status. Results: ER positivity was associated with small size, lobulate, angular or spiculated margin contours, absence of calcification, posterior tumor shadowing and low elasticity score; PR positivity was associated with small size, lobulate or angular or spiculated margin contours and absence of calcification; HER2 positivity was associated with presence of calcification and absence of any echogenic halo. The calculated models of predicted molecular status were accurate and discriminating with AUCs of 0.78, 0.74, and 0.74, respectively. Conclusions: Breast cnacer ultrasound features show some correlation with the molecular status. These models may help to expand the scope of ultrasound in predicting tumor biology.

A New Triterpenoid from Brucea javanica

Jian Hua Liu,Jiang Jiang Qin,Hui Zi Jin,Xiao Jia Hu,Ming Chen,Yun Heng Shen,Shi Kai Yan,Wei Dong Zhang 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.5

A new triterpenoid, bruceajavanin C (1), together with bruceosides A and B (2 and 3), bruceines D and E (4 and 5), yadanziosides A and G (6 and 7), (20R)-O-(3)-α-L-arabinopyranosylpregn- 5-ene-3β,20-diol (8), and α-D-glucopyranoside, (3β, 20R)-3-hydroxypregn-5-en-20-yl (9) were isolated from the aerial parts of Brucea javanica. The structure of 1 was elucidated on the basis of 2D-NMR spectroscopic analysis. In addition, compounds 1, 3, 4, 5, and 6 exhibited mild inhibitory effect on NO production in LPS-stimulated RAW264.7 cells.

Cloning and expression pattern of odorant receptor 11 in Asian honeybee drones, Apis cerana (Hymenoptera, Apidae)

Jun Feng Liu,Le Yang,Mang Li,Xu Jiang He,Zi-long Wang,Zhijiang Zeng 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.1

Odorant receptors play a crucial role in the special recognition of scent molecules in the honeybee olfaction system. The odorant receptor 11 (AmOR11) in western honeybee drones (Apis mellifera) has been demonstrated to specifically bind to 9-oxo-2-decenoic acid (9-ODA) of queens. However, little is known regarding the functions of OR11 Asian honeybee drones (Apis cerana) in the context of their mating activities. In this study, the odorant receptor 11 gene (AcOr11) from A. cerana was cloned, and its expression profiles were examined during two developmental stages (immature and sexually mature) and different physiological statuses (flying and crawling). The cDNA sequence of AcOr11 was highly similar to that of AmOr11, and encoded a membrane-coupled protein of 384 amino acids. The results of qRT-PCR indicated that AcOr11 was expressed at higher levels in drone antennae compared to brains, and the expression was significantly up-regulated in sexually mature drone brains compared to immature brains. Interestingly, AcOr11 expression in brains of mature flying drones was dramatically higher than those of mature crawling drones. To our knowledge, this study demonstrate a link between AcOr11 gene expression in the brain of honeybee drones and behavior associated with sexual maturity and mating flight.

DFT and TD-DFT study on structure and properties of organic dye sensitizer TA-St-CA

Cai-Rong Zhang,Zi-Jiang Liu,Yu-Hong Chen,Hong-Shan Chen,You-Zhi Wu,WangJun Feng,Dao-Bin Wang 한국물리학회 2010 Current Applied Physics Vol.10 No.1

The geometry, electronic structure, polarizability and hyperpolarizability of organic dye sensitizer TA-St-CA, which contains a π-conjugated oligo-phenylenevinylene unit with an electron donor.acceptor moiety, was studied using density functional theory (DFT), and the electronic absorption spectrum was investigated via time-dependent DFT (TD-DFT) with several hybrid functionals. The calculated geometry indicates that the strong conjugated effects are formed in the dye. The TD-DFT results show that the hybrid functional PBE1PBE and MPW1PW91 are more suitable than B3LYP for calculating electronic absorption spectra. The features of electronic absorption spectra were assigned on account of the qualitative agreement between the experiment and the calculations. The absorption bands in visible and near-UV region are related to photoinduced electron transfer processes, and the diphenylaniline group is major chromophore that contributed to the sensitization, and the interfacial electron transfer are electron injection processes from the excited dyes to the semiconductor conduction band. Compared with the similar dye D5, the good performance of TA-St-CA in dye-sensitized solar cells may be resulted from the higher energy level of the lowest unoccupied molecular orbital and the larger oscillator strengths for the most excited states with intramolecular electron transfer character.

Impact of Size on Humidity Sensing Property of Copper Oxide Nanoparticles

Yang Gu,Huina Jiang,Zi Ye,Ning Sun,Xuliang Kuang,Weijing Liu,Gaofang Li,Xiaojun Song,Lei Zhang,Wei Bai,Xiaodong Tang 대한금속·재료학회 2020 ELECTRONIC MATERIALS LETTERS Vol.16 No.1

Three sizes of CuO nanosheets were synthesized by hydrothermal method. The structure and morphology of CuO nanosheets were characterized by X-ray difraction and scanning electron microscopy. Dielectrophoresis nano-manipulation technique was employed to arrange the materials on pre-designed Ti/Au electrodes to fabricate the three humidity sensors, and the sensing properties were then tested. The experimental results show that the sensitivity greatly increases with the decreasing size of CuO nanosheets, the sensitivity of sensor a, b, c are 369%, 3278%, 22,611% in 97.3% RH, respectively. The smaller sized CuO nanomaterials have better response characteristic, the response time of sensor a, b, c under 11.3–97.3% RH are 53 s, 49 s, 32 s, respectively. And correspondingly, hysteresis properties and the repeatability are also a little infuenced. In addition, based on complex impedance spectroscopy and multilayer adsorption theory, the impact of size on humidity sensing property was discussed. The results indicated the feasibility to obtain higher performance of humidity sensor, especially the higher sensitivity, via employment the smaller size sensing nanomaterials.