Nicotinamide Mononucleotide Adenylyl Transferase 2 Inhibition Aggravates Neurological Damage after Traumatic Brain Injury in a Rat Model

Xiaoyu Gu,Haibo Ni,XuGang Kan,Chen Chen,Zhiping Zhou,Zheng Ding,Di Li,Bofei Liu 대한신경외과학회 2023 Journal of Korean neurosurgical society Vol.66 No.4

Objective : Nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2) is a crucial factor for the survival of neuron. The role of NMNAT2 in damage following traumatic brain injury (TBI) remains unknown. This study was designed to investigate the role of NMNAT2 in TBI-induced neuronal degeneration and neurological deficits in rats. Methods : The TBI model was established in Sprague-Dawley rats by a weight-dropping method. Real-time polymerase chain reaction, western blot, immunofluorescence, Fluoro-Jade C staining, and neurological score analyses were carried out. Results : NMNAT2 mRNA and protein levels were increased in the injured-side cortex at 6 hours and peaked 12 hours after TBI. Knocking down NMNAT2 with an injection of small interfering RNA in lateral ventricle significantly exacerbated neuronal degeneration and neurological deficits after TBI, which were accompanied by increased expression of BCL-2-associated X protein (Bax). Conclusion : NMNAT2 expression is increased and NMNAT2 exhibits neuroprotective activity in the early stages after TBI, and Bax signaling pathway may be involved in the process. Thus, NMNAT2 is likely to be an important target to prevent secondary damage following TBI.

Factors Associated with Behaviors Toward End-of-life Care Among Chinese Oncology Nurses: A Cross-Sectional Study

Xiaoyu Wu,Zhihuan Zhou,Yiheng Zhang,Xiaoyan Lin,Meng Zhang,Fulin Pu,Meifen Zhang 한국간호과학회 2021 Asian Nursing Research Vol.15 No.5

Purpose The goal of this study was to describe the current status of oncology nurses' behaviors toward end of life (EOL) care in China and to explore the factors associated with oncology nurses’ behaviors toward EOL care. Methods A cross-sectional design was applied and a convenience sample of 1038 oncology nurses from 22 grade A hospitals were recruited into this study. A general social demographic data questionnaire was administered, and the Chinese version of Nurses’ Behaviors of Caring for Dying Patients Scale was used to assess nurse behavior toward EOL care. The total score ranges from 40 to 200 points. Data were analyzed with SPSS 26.0 software. Results Chinese oncology nurses' average score of holistic EOL care behaviors was 2.97 ± 0.59. Oncology nurses provide physical care most (3.81 ± 0.76), followed by family care (3.02 ± 0.86), and spiritual care (2.37 ± 0.67). Multiple regression analysis showed that a higher frequency of sharing EOL care experience with colleagues, in-service palliative care education, higher level of head nurse support for EOL patient care, more cases of EOL care, higher working position, and nurse's perceived high level of support were positively associated with behavior toward EOL care. These six factors explained 16.2% of the total variance. Conclusions The results may help provide a basis for converting behavior for EOL care among oncology nurses and design interventions to better improve quality of life for EOL patients with cancer in China. Purpose The goal of this study was to describe the current status of oncology nurses' behaviors toward end of life (EOL) care in China and to explore the factors associated with oncology nurses’ behaviors toward EOL care. Methods A cross-sectional design was applied and a convenience sample of 1038 oncology nurses from 22 grade A hospitals were recruited into this study. A general social demographic data questionnaire was administered, and the Chinese version of Nurses’ Behaviors of Caring for Dying Patients Scale was used to assess nurse behavior toward EOL care. The total score ranges from 40 to 200 points. Data were analyzed with SPSS 26.0 software. Results Chinese oncology nurses' average score of holistic EOL care behaviors was 2.97 ± 0.59. Oncology nurses provide physical care most (3.81 ± 0.76), followed by family care (3.02 ± 0.86), and spiritual care (2.37 ± 0.67). Multiple regression analysis showed that a higher frequency of sharing EOL care experience with colleagues, in-service palliative care education, higher level of head nurse support for EOL patient care, more cases of EOL care, higher working position, and nurse's perceived high level of support were positively associated with behavior toward EOL care. These six factors explained 16.2% of the total variance. Conclusions The results may help provide a basis for converting behavior for EOL care among oncology nurses and design interventions to better improve quality of life for EOL patients with cancer in China.

Pseudolaric Acid B Inhibits Proliferation, Invasion and Epithelial-to-Mesenchymal Transition in Human Pancreatic Cancer Cell

Xiaoyu Li,Xianzhi Zhao,Wen Song,Zibin Tian,Lin Yang,Qinghui Niu,Qi Zhang,Man Xie,Bin Zhou,Yonghong Xu,Jun Wu,Cuiping Zhang 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.1

Purpose: This study was aimed to investigate the effect of pseudolaric acid B (PAB) on proliferation, invasion and epithelial-to-mesenchymal transition (EMT) in pancreatic cancer cells and to explore the possible mechanism. Materials and Methods: The pancreatic cancer cell line SW1990 was cultured and treated with PAB dose- and time-dependent manners. Cell proliferation and invasion ability were measured by MTT assay and Matrigel/Transwell test, respectively. Semi-quantitative real-time polymerase chain reaction and Western blotting were conducted to detect the expression of EMT markers and the key molecules. Finally, nude mice subcutaneous transplantation tumor model was used to confirm the therapy efficacy of PAB. Results: PAB could inhibit SW1990 cell proliferation and invasion in time- and dose-dependent manners. Vimentin, fibronectin, N-cadherin, Snail, Slug, YAP, TEAD1, and Survivin were down-regulated (p<0.01), while E-cadherin, caspase-9, MST1, and pYAP were up-regulated (p<0.05). Combined PAB and gemcitabine treatment markedly restricted the tumor growth compared with gencitabin or PAB alone groups. Conclusion: PAB could inhibit the proliferation and invasion ability of pancreatic cancer cells through activating Hippo-YAP pathway and inhibiting the process of EMT.

A study on the effect of user experience of fitness APP on product trust and purchase intention

Huizhuo Zhou,Xiaoyu Xing,Zifan Lu 강원대학교 경영경제연구소 2022 Asia-Pacific Journal of Business Vol.13 No.3

Purpose - The purpose of this study is to take fitness APP users as the research object from the perspective of user experience to explore the influence of fitness APP user experience factors on product trust and purchase intention. Design/methodology/approach - The study collected data on 275 customers who had experience buying and using fitness apps. To test the hypothesis, SPSS 27.0 and AMOS 26.0 statistical packages were used based on the collected data. Findings - The results showed that the user experience factors (usefulness, ease to use, enjoyment, interaction) of fitness APP and the relationship between product trust had a positive effect, and product trust had a positive effect on purchase intention. In addition, exercise experience, showed a moderating effect in the relationship between the usefulness, easy to use of user experience and product trust. Research implications or Originality - This study provided research model among user experience factors of fitness APP, product trust and purchase intention. This study can help sports and fitness companies with product optimization and marketing decisions.

Research on the Method for Periodic Estimation of the PN Sequence in the Lower SNR DS/SS Signals

Fan Zhou,Yongxin Feng,Xiaoyu Zhang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.10

The periodic estimation method of the PN sequence in DS Signal is researched. It is hard for traditional methods to estimate PN period of DS/SS accurately in the condition of low Signal-to-noise ratio. On the basis of the traditional spectrum reprocessing and the cepstrum method, a PN period estimation method which has integrated serial average with spectrum reprocessing and the cepstrum are put forward to solve this problem and meet the requirements of lower Signal-to-noise ratio. The simulation results show that, when the period of PN sequence is estimated accurately, the cepstrum method has better estimating performance than the spectrum reprocessing method, and the spectral estimation method has lower Signal-to-noise ratio tolerance after improvements.

The Mechanism of Rockbolt in the Jointed Rock Under Uniaxial Tension

Shen Zhou,Xiaoyu Ji,Li-Ping Li,Hong-liang Liu,Chun Zhu,Hongyun Fan,Qi Zhang,Caihua Shi,Xutong Zhang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.11

In this research, the rockbolt mechanism of a jointed rock mass under uniaxial tension was systematically revealed with a laboratory test and a numerical simulation. It was found that the rockbolt rock mass experienced five stages under uniaxial tension, the densification stage, elastic stage, plastic deformation stage, progressive debonding stage, and complete debonding stage. The stress-strain curve and ultimate tensile strength of a rockbolt rock mass were analyzed by taking the rockbolt spacing and rockbolt angle as variables. It was found that the improvement effect of the reduction of the rockbolt spacing on the ultimate tensile strength was limited. When the rockbolt spacing was reduced to a certain limit, the stress concentration area between adjacent rockbolts was connected and destroyed, resulting in the increase of the rockbolt rock strength becoming smaller, and even having a downward trend. The increase of the rockbolt angle led to the change of the stress mode and failure mode of the whole structure, and the ultimate tensile strength first increased and then decreased. The optimal rockbolt angle was between 60° and 70°. It is worth noting that there was an obvious mechanical occlusion between the thread on the rockbolt surface and the rock mass, resulting in the multi-stage step-down characteristic of the stress-strain curve in the complete debonding stage. The results of this study can provide a reference for the design and construction of similar projects.

High-temperature steam oxidation behaviors of a ZrO₂/CrAlSi composite coating on Zr-2 alloy deposited by PEO duplex FCVA treatments

Qian Zhou,Yizhao Liao,Hui Li,Hu Chen,Chi Xu,Xiaoyue Jin,Wenbin Xue 한국표면공학회 2023 한국표면공학회 학술발표회 초록집 Vol.2023 No.11-1

Comparative transcriptome analysis to identify genes involved in terpenoid biosynthesis in Agriophyllum squarrosum, a folk medicinal herb native to Asian temperature deserts

Yin Xiaoyue,Yan-Xia Liu,Qian Chaoju,Zhou Shanshan,Fang Tingzhou,Fan Xingke,Gao yuan,Chang Yuxiao,Yang Jian,Ma Xiao-Fei 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.3

Agriophyllum squarrosum is a folk Mongolian medicine with pleiotropic pharmacological and ecological economic importance endemic to Asian temperature deserts. Terpenoids play critical roles in biotic and abiotic stresses due to their antioxidative activities. Based on non-targeted metabolomic analysis, we detected eight terpenoids enriched in the above-ground tissues of A. squarrosum, however, the molecular mechanism underlying terpenoids biosynthesis in this desert medicinal plant is rarely understood. Here, a comparative transcriptome analysis of diferent tissues in A. squarrosum was conducted to identify 84 unigenes encoding key enzymes in the upstream backbone biosynthesis and 53 unigenes encoding the downstream enzymes for terpenoid diversifcation. Most of the upstream genes exhibited signifcant high expression levels in leaf, and some of which were validated by qRT-PCR. Phylogenetic analysis showed that two downstream gene families OSCs (oxidosqualene cyclases) and TPSs (mainly in terpene synthases -g subfamily) had undergone notable gene expansions in A. squarrosum comparing with the other Amaranthaceae plant species and Arabidopsis. Nevertheless, most members from these two gene families showed the tissue-specifc expression in A. squarrosum, which supported the diversifcation and tissue-specifc enrichment of terpenoids across above-ground tissues. Considering to the habitat characteristics of A. squarrosum, we proposed that the enrichment of terpenoids and the functional diversifcation of terpenoids biosynthesis enzymes were more or less involved into its adaptation to stressful environments of deserts. These results expand the available genetic information underlying terpenoid biosynthesis in A. squarrosum, and contribute to deeper researches on pharmaceutical and eco-agricultural applications in this desert medicinal plant.

Study on the Impact of Fresh Food E-Commerce Logistics Service Quality on Customer Satisfaction, Customer Trust, and Repurchase Intention

Huizhuo Zhou,Xiaoyu Xing 한국무역연구원 2022 무역연구 Vol.18 No.5

Purpose – This study is meaningful in providing strategic implications for improving the logistics service quality of fresh food e-commerce companies by empirically analyzing the impact of logistics service quality on customer satisfaction, customer trust, and repurchase intention. Design/Methodology/Approach – The study collected data on 285 customers with shopping experience on fresh food e-commerce platforms and verified with a structural equation model using AMOS 26.0. Findings – First, it was confirmed that the delivery quality, timeliness, and reliability of fresh food e-commerce logistics service quality had a positive impact on customer satisfaction. Second, it was found that delivery quality, information quality, and reliability of fresh food e-commerce logistics service quality had a positive impact on customer trust. Third, both customer satisfaction and customer trust had a positive impact on repurchase intention. Research Implications – In terms of theory, theoretical research is carried out by investigating which factors of fresh food e-commerce logistics service quality can improve customer satisfaction and customer trust. In terms of practice, it provides reference suggestions for fresh food e-commerce enterprises to improve the quality of logistics services and improve business performance in the future. It will also provide a reference for other follow-up research.

Analysis on international competitiveness in large and medium-sized high-tech state-owned enterprises for Tianjin optical-mechanical-electrical integrated industries

Xu Deling,Zhou Hong,Li Xiaoyu,Guo Ying 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

According to research on optical-mechanical-electrical integrated industry in Tianjin, the paper has been applying for domestic and international measurements drawing on the international competitiveness to conduct several achievements on the following fields. such as designing a index system to analyze international competitiveness of large and medium-sized high-tech state-owned enterprises in Tianjin, utilizing AHP and Illegibility Model to evaluate international competitiveness in optical-mechanical-electrical integrated industries of large and medium-sized state-owned enterprises in Tianjim so as to promote some proper countermeasures.