Biotransformation of Panax ginseng extract by rat intestinal microflora

Wei-Wei Dong,Jinhua Zhao,Fei-Liang Zhong,Wen-Jing Zhu,Jun Jiang,Songquan Wu,Deok-Chun Yang,Donghao Li,Lin-Hu Quan 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.4

Background: In general, after Panax ginseng is administered orally, intestinal microbes play a crucial role in its degradation and metabolization process. Studies on the metabolism of P. ginseng by microflora are important for obtaining a better understanding of their biological effects. Methods: In vitro biotransformation of P. ginseng extract by rat intestinal microflora was investigated at 37C for 24 h, and the simultaneous determination of the metabolites and metabolic profile of P. ginseng saponins by rat intestinal microflora was achieved using LCeMS/MS. Results: A total of seven ginsenosides were detected in the P. ginseng extract, including ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd. In the transformed P. ginseng samples, considerable amounts of deglycosylated metabolite compound K and Rh1 were detected. In addition, minimal amounts of deglycosylated metabolites (ginsenosides Rg2, F1, F2, Rg3, and protopanaxatriol-type ginsenosides) and untransformed ginsenosides Re, Rg1, and Rd were detected at 24 h. The results indicated that the primary metabolites are compound K and Rh1, and the protopanaxadiol-type ginsenosides were more easily metabolized than protopanaxatriol-type ginsenosides. Conclusion: This is the first report of the identification and quantification of the metabolism and metabolic profile of P. ginseng extract in rat intestinal microflora using LCeMS/MS. The current study provided new insights for studying the metabolism and active metabolites of P. ginseng.

Regeneration and Recycling of Waste Thermosetting Plastics based on Mechanical Thermal Coupling Fields

Zhong-Wei Wu,Shaobo Pan,Guang-Fu Liu,Shou-Xu Song 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

In view of the present situation that the recovery of waste thermosetting plastics and products is difficult and the utilization rate islow, regeneration mechanism, regeneration methods and recycling process of waste thermosetting plastics under mechanical thermalcoupling fields are proposed. Regeneration powders of Thermosetting Phenolic plastic are obtained by mechanochemical activatedregeneration methods, the molecular structure, properties and crosslink density of phenol regeneration materials are analyzed. Analysis results show that network crosslinked molecular structure was destroyed, reactive groups produced and the crosslink densitydecreased. The activity of regeneration powders was reinforced and its plasticity was also improving, so the utilization rate wasimproved. Regeneration composite plastics were manufactured by hot press molding or extrusion compression molding. Themicrostructure of regeneration composite plastics was analyzed, and its mechanical strength was tested. Results showed that thetensile strength and bending strength of samples (weight ratio ≥ 50%) respectively exceed 15.88 MPa and 33.49 MPa. At last processparameters for regeneration effects were discussed, and these parameters were optimized. When particle size was 80 mesh (size ≤ 0.18mm), quality proportion was 50~70%, pressure was 10Mpa, heating temperature was 180~190oC, the optimal effects for regeneratedcomposite were achieved.

Biotransformation of Panax ginseng extract by rat intestinal microflora: identification and quantification of metabolites using liquid chromatography-tandem mass spectrometry

Dong, Wei-Wei,Zhao, Jinhua,Zhong, Fei-Liang,Zhu, Wen-Jing,Jiang, Jun,Wu, Songquan,Yang, Deok-Chun,Li, Donghao,Quan, Lin-Hu The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.4

Background: In general, after Panax ginseng is administered orally, intestinal microbes play a crucial role in its degradation and metabolization process. Studies on the metabolism of P. ginseng by microflora are important for obtaining a better understanding of their biological effects. Methods: In vitro biotransformation of P. ginseng extract by rat intestinal microflora was investigated at $37^{\circ}C$ for 24 h, and the simultaneous determination of the metabolites and metabolic profile of P. ginseng saponins by rat intestinal microflora was achieved using LC-MS/MS. Results: A total of seven ginsenosides were detected in the P. ginseng extract, including ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd. In the transformed P. ginseng samples, considerable amounts of deglycosylated metabolite compound K and Rh1 were detected. In addition, minimal amounts of deglycosylated metabolites (ginsenosides Rg2, F1, F2, Rg3, and protopanaxatriol-type ginsenosides) and untransformed ginsenosides Re, Rg1, and Rd were detected at 24 h. The results indicated that the primary metabolites are compound K and Rh1, and the protopanaxadiol-type ginsenosides were more easily metabolized than protopanaxatriol-type ginsenosides. Conclusion: This is the first report of the identification and quantification of the metabolism and metabolic profile of P. ginseng extract in rat intestinal microflora using LC-MS/MS. The current study provided new insights for studying the metabolism and active metabolites of P. ginseng.

Expression of High Mobility Group Box - B1 (HMGB-1) and Matrix Metalloproteinase-9 (MMP-9) in Non-small Cell Lung Cancer (NSCLC)

Wang, Jing-Luan,Wu, Da-Wei,Cheng, Zhao-Zhong,Han, Wei-Zhong,Xu, Sheng-Wei,Sun, Ni-Na Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Objective: This study evaluated the expression level of high mobility group box-B1 (HMGB-1) and matrix metalloproteinase-9 (MMP-9) in non-small cell lung cancer (NSCLC) inmorder to reveal any relation with development and prognosis. Methods: NSCLC and normal tissues were selected from 30 patients at age of 30-73, and used for RT-PCR and Western blot analyses of HMGB-1. A total of 100 paraffin embedded NSCLC tissues were also isolated from patients through surgical resection, and used for detection of HMGB-1 by immunohistochemistry. In addition, 50 samples were also applied for MMP-9 detection, and 30 normal tissues were considered as controls. Correlation analysis of HMGB-1 and MMP-9 was carried out by Pearsons correlation coefficient. Results: The average expression level of HMGB-1 in NSCLC patients was significantly higher than in normal lung tissues. In addition, patients in III-IV period exhibit significantly higher positive rate of HMGB-1 when compared with I-II period cases. Furthermore, a positive correlation with HMGB-1 was found in the expression of MPP-9. Conclusion: HMGB-1 was highly expressed in NSCLC, which may become a prognostic and predictive marker for NSCLC. Besides, MPP-9 was positively correlated with HMGB-1.

Metagenomics analysis of the gut microbiome in healthy and bacterial pneumonia forest musk deer

Wei Zhao,Ziwei Ren,Yan Luo,Jianguo Cheng,Jie Wang,Yin Wang,Zexiao Yang,Xueping Yao,Zhijun Zhong,Wei Yang,Xi Wu 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background The forest musk deer (FMD, Moschus berezovskii) is an threatened species in China. Bacterial pneumonia was found to seriously restrict the development of FMD captive breeding. Historical evidence has demonstrated the relationship between immune system and intestinal Lactobacillus in FMD. Objective We sought to elucidate the diferences in the gut microbiota of healthy and bacterial pneumonia FMD. Methods The bacterial pneumonia FMD was demonstrated by bacterial and pathological diagnosis, and the gut microbiome of healthy and bacterial pneumonia FMD was sequenced and analysed. Results There are three pathogens (Pseudomonas aeruginosa, Streptococcus equinus and Trueperella pyogenes) isolated from the bacterial pneumonia FMD individuals. Compared with the healthy group, the abundance of Firmicutes and Proteobacteria in the pneumonia group was changed, and a high level of Proteobacteria was found in the pneumonia group. In addition, a higher abundance of Acinetobacter (p=0.01) was observed in the population of the pneumonia group compared with the healthy group. Several potentially harmful bacteria and disease-related KEGG subsystems were only found in the gut of the bacterial pneumonia group. Analysis of KEGG revealed that many genes related to type IV secretion system, type IV pilus, lipopolysaccharide export system, HTH-type transcriptional regulator/antitoxin MqsA, and ArsR family transcriptional regulator were signifcantly enriched in the metagenome of the bacterial pneumonia FMD. Conclusion Our results demonstrated that the gut microbiome was signifcantly altered in the bacterial pneumonia group. Overall, our research improves the understanding of the potential role of the gut microbiota in the FMD bacterial pneumonia.

Clinical Application of Endoscopic Inguinal Lymph Node Resection after Lipolysis and Liposuction for Vulvar Cancer

Wu, Qiang,Zhao, Yi-Bing,Sun, Zhi-Hua,Ni, Jing,Wu, Yu-Zhong,Shao, Heng-Hua,Qu, Jun-Wei,Huang, Xin-En Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Aim: To examine lymph nodes obtained after lipolysis and liposuction of subcutaneous fat of the inguinal region of female vulvar cancer patients to explore the feasibility of clinical application. Methods: The field of operation was on the basis of the range of the conventional resection of inguinal lymph nodes. We injected lipolysis liquid fanwise, started liposuction after 15-20 minutes; then the subcutaneous fatty tissue was sucked out clearly by suction tube. We selected the first puncture holes located on 2-3 cm part below anterior superior spine, the others respectively being located 3cm and 6cm below the first for puncturing into the skin, imbedding a trocar to intorduce $CO_2$ gas and the specular body, and excise the lymph nodes by ultrasonic scalpel. The surgical field chamber was set with negative pressure drainage and was pressured with a soft saline bag after surgery. Results: A lacuna emerged from subcutaneous of the inguinal region after lipolysis and liposuction, with a wide fascia easily exposed at the bottom where lymph nodes could be readily excised. The number of lymph nodes of ten patients excised within the inguinal region on each side was 4-18. The excised average number of lymph nodes was 11 when we had mature technology. Conclusion: Most of adipose tissue was removed after lipolysis and liposuction of subcutaneous tissue of inguinal region, so that the included lymph nodes were exposed and easy to excise by endoscope. This surgery avoided the large incision of regular surgery of inguinal region, the results indicating that this approach is feasible and safe for used as an alternative technology.

Time-dependent Nonlinear Theory and Numerical Simulation of Folded Waveguide Traveling Wave Tubes

Wei Feng Peng,Zhong Hai Yang,Yu Lu Hu,Zan Cao,Yin-Fu Hu,Jin-Jun Feng,Xian-Ping Wu 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.62 No.6

In this paper, a time-dependent nonlinear theory including the generalized time-dependent radiofrequency (RF) field equations is presented to simulate the beam and wave interaction (BWI) of folded waveguide (FWG) traveling wave tubes (TWTs). The analytical RF fields in FWG TWTs are replaced by digitized RF field profiles obtained from electromagnetic simulations. A W-band FWG TWT is studied by using a self-consistent one-dimensional code based on the theory. The numerical results show good predictions when compared with the experimental tests.

Comprehensive Evaluation of Impacts of Connecting Distributed Generation to the Distribution Network

Wei Jin,Xuemei Shi,Fei Ge,Wei Zhang,Hongbin Wu,Chengyuan Zhong 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.2

In this paper, we study the various impacts of connecting distributed generation (DG) to the distribution network. The comprehensive evaluation index system (CEIS) of four hierarchies is established, considering economy, reliability and voltage quality, and the calculation methods of different indexes are presented. This paper puts forward an improved triangular fuzzy number analytic hierarchy process (ITFNAHP) to weight the second level indexes (SLI) and the third level indexes (TLI), and calculates the variation coefficient to weight the fourth level indexes (FLI). We calculate the comprehensive weight coefficients based on the weight coefficients of the SLI, TLI and FLI, and then calculate the comprehensive evaluation of satisfaction (CES) of different access schemes. On the basis of the IEEE 33-bus example system, simulations of the calculation methods and the comprehensive evaluation method are carried out under different DG access schemes according to the same total investment cost and the same permeability, respectively, and the simulation results are analyzed and discussed.

Biotransformation of gypenoside XVII to compound K by a recombinant β-glucosidase

Zhong, Fei-Liang,Dong, Wei-Wei,Wu, Songquan,Jiang, Jun,Yang, Deok-Chun,Li, Donghao,Quan, Lin-Hu Springer-Verlag 2016 Biotechnology letters Vol.38 No.7

An Optimization Model on Virtual Machines Allocation Based on Radial Basis Function Neural Networks

Wei Wu,Wencai Du,Hui Zhou,Jiezhuo Zhong,Zhen Guo 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.6

Properly allocation of virtual machines is important for computing infrastructures scheduling. This paper presents systemic method on virtual machine array optimization control based on artificial intelligence and matrix control theory. According to request service data from users to provide proper VMs roughly via intelligent pattern recognition based on RBFNN, the data is sent to a multiple-targets optimization process to produce VMs allocation matrix precisely, thus enable to minimize the cast and enhance efficiency of the whole array to achieve low consumption optimization and ensure the stability of the system. Simulation experiments confirmed the effectiveness of this model and adaption ability in online dynamics.