Agromyces laixinhei sp. nov. isolated from bat feces in China

Cheng Yanpeng,Bai Yibo,Huang Yuyuan,Yang Jing,Lu Shan,Jin Dong,Pu Ji,Zheng Han,Li Junqin,Huang Ying,Wang Suping,Xu Jianguo 한국미생물학회 2021 The journal of microbiology Vol.59 No.5

Three rod-shaped, Gram-stain-positive, and catalase-positive, phenotypically closely related isolates (HY052T, HY050, and HY045) were obtained from fecal samples collected from bats in Guangxi province and Chongqing city of China. Circular, smooth, light-yellow colonies appeared on brain heart infusion plate after 24–48 h incubation at 28°C. The optimal pH for growth was between 6.0 and 7.5. Based on 16S rRNA, the three isolates were phylogenetically related to Agromyces terreus DS-10T, Agromyces aureus AR33T, Agromyces salentinus 20-5T, Agromyces allii UMS-62T, Agromyces lapidis CD55T, and Agromyces italicus CD1T. Moreover, based on 296 core genes, the phylogenomic tree indicated that the three isolates clustered together, closest to Agromyces cerinus VKM Ac- 1340T and Agromyces fucosus VKM Ac-1345T but separated distantly from other Agromyces species. The average nucleotide identity values between strain HY052T and other Agromyces species ranged from 79.3% to 87.9%, lower than the 95–96% threshold. Furthermore, the genome of strain HY052T contains a circular chromosome of 3,437,203 bp with G + C content of 69.0 mol%. Main fatty acids were anteiso-C15:0 and anteiso-C17:0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, and unidentified glycolipids. Rhamnose, ribose, and glucose were the primary cell wall sugars. The major peptidoglycan amino acids included alanine, glutamic acid, glycine, and 2,4-diaminobutyric acid. An additional remarkable difference from other Agromyces species is that MK-12 was the sole menaquinone in strain HY052T. Based on results from the polyphasic characterizations performed in this study, our isolates are proposed to be members of a novel species in genus Agromyces, named Agromyces laixinhei. The type strain is HY052T (= CGMCC 1.17175T = JCM 33695T).

NUMERICAL STUDY ON THE SHAPED CHARGES

TIANBAO MA,CHENG WANG,JIANGUO NING 한국소성가공학회 2008 한국소성가공학회 컨퍼런스 Vol.2008 No.10

Genome sequence of lung pathogenic Escherichia coli O78, a chimeric strain isolated from pneumonia forest musk deer

Qing Tian,Xin Zhou,Jianguo Cheng,Yan Luo,Lei Dai,Wei Zhao,Wuyou Wang 한국유전학회 2017 Genes & Genomics Vol.39 No.7

Pneumonia is one of the major diseases of forest musk deer (FMD) that affects the survival of musk deer breeding farms. Lung pathogenic Escherichia coli (LPEC) strains were found to be one of the principal bacterial pathogens, and O78 was found to be the dominant serotype and the most poisonous, thus it was selected as further study. Here we have finished the LPEC O78 genome sequence, genomic comparative analysis and genome annotation. In the genome of LPEC O78, genes encoded major virulence factors (VFs) of urinary tract infection (UTI) and neonatal meningitis-causing E. coli (NMEC) were detected. The phylogenetic analysis indicated that LPEC O78 belongs to the D group of E. coli and clustered with human uropathogenic E. coli (UPEC) UMNO26. Orthologous analysis showed that LPEC O78 was close to UMNO26 in evolutionary relationships, the results were consistent with phylogenetic analysis. Additionally, analysis of the specific genes using COG, GO and Swiss-Prot databases revealed specific functions in the LPEC O78, some of these differences might reflect the pathogenicity of LPEC O78. It was the first time that LPEC from FMD was sequenced, through the virulence gene analysis, an example of a genome of chimeric pathogenic properties was found. The results were in favor of the possibility that strain of animal origin LPEC O78 was dangerous for public health and consequently constitute a zoonotic risk. Moreover, the functions study of specific genes will facilitate understand the pathogenicity of LPEC O78, and aid in the development of control measures.

Piezoelectric properties of [Li0.03(K0.48Na0.52)0.97](Nb0.97Sb0.03)O3-(Ba0.85Ca0.15)(Ti0.90Zr0.10)O3 lead-free piezoelectric ceramics

Xiaoyu Chen,Jiagang Wu,Xiaojing Cheng,Bo Wu,Wenjuan Wu,Dingquan Xiao,Jianguo Zhu 한국물리학회 2012 Current Applied Physics Vol.12 No.3

[Li0.03(K0.48Na0.52)0.97](Nb0.97Sb0.03)O3-(Ba0.85Ca0.15)(Ti0.90Zr0.10)O3 [(1-x)LKNNS-xBCTZ] lead-free piezoelectric ceramics were prepared by the conventional solid state method, and effects of BCTZ content on the piezoelectric properties of LKNNS ceramics were mainly investigated. A stable solid solution has been formed between LKNNS and BCTZ, and a morphotropic phase boundary of (1-x)LKNNS-xBCTZ ceramics is identified in the range of 0 < x ≤ 0.02. The Curie temperature of (1-x)LKNNS-xBCTZ ceramics decreases with increasing BCTZ content. A higher εr value and a lower tan d value are demonstrated for the (1-x)LKNNS-xBCTZ ceramic with x = 0.02. The (1-x)LKNNS-xBCTZ ceramic with x = 0.02 has an enhanced electrical behavior of d33w237 pC/N, kpw 48.6%, 3 rw1451, tan d w0.037, and Tcw335 ℃. As a result, (1-x)LKNNS-xBCTZ ceramics are promising candidate materials for the field of lead-free piezoelectric materials.

Metagenomics analysis of the gut microbiome in healthy and bacterial pneumonia forest musk deer

Wei Zhao,Ziwei Ren,Yan Luo,Jianguo Cheng,Jie Wang,Yin Wang,Zexiao Yang,Xueping Yao,Zhijun Zhong,Wei Yang,Xi Wu 한국유전학회 2021 Genes & Genomics Vol.43 No.1

Background The forest musk deer (FMD, Moschus berezovskii) is an threatened species in China. Bacterial pneumonia was found to seriously restrict the development of FMD captive breeding. Historical evidence has demonstrated the relationship between immune system and intestinal Lactobacillus in FMD. Objective We sought to elucidate the diferences in the gut microbiota of healthy and bacterial pneumonia FMD. Methods The bacterial pneumonia FMD was demonstrated by bacterial and pathological diagnosis, and the gut microbiome of healthy and bacterial pneumonia FMD was sequenced and analysed. Results There are three pathogens (Pseudomonas aeruginosa, Streptococcus equinus and Trueperella pyogenes) isolated from the bacterial pneumonia FMD individuals. Compared with the healthy group, the abundance of Firmicutes and Proteobacteria in the pneumonia group was changed, and a high level of Proteobacteria was found in the pneumonia group. In addition, a higher abundance of Acinetobacter (p=0.01) was observed in the population of the pneumonia group compared with the healthy group. Several potentially harmful bacteria and disease-related KEGG subsystems were only found in the gut of the bacterial pneumonia group. Analysis of KEGG revealed that many genes related to type IV secretion system, type IV pilus, lipopolysaccharide export system, HTH-type transcriptional regulator/antitoxin MqsA, and ArsR family transcriptional regulator were signifcantly enriched in the metagenome of the bacterial pneumonia FMD. Conclusion Our results demonstrated that the gut microbiome was signifcantly altered in the bacterial pneumonia group. Overall, our research improves the understanding of the potential role of the gut microbiota in the FMD bacterial pneumonia.

Rhombohedraleorthorhombic phase coexistence and electrical properties of Ta and BaZrO3 co-modified (K, Na)NbO3 lead-free ceramics

Binyu Zhang,Jiagang Wu,Xiaopeng Wang,Xiaojing Cheng,Jianguo Zhu,Dingquan Xiao 한국물리학회 2013 Current Applied Physics Vol.13 No.8

The BaZrO3 and Ta have been used to improve piezoelectric properties of (K, Na)NbO3 ceramics by the construction of the phase boundary, and (1 x)K0.48Na0.52(Nb0.95Ta0.05)O3exBaZrO3 [(1 x)KNNTexBZ]ceramics were prepared by the conventional solid-state method. The effect of BZ content on their phase structure, microstructure, and electrical properties has been investigated. A rhombohedral and orthorhombic phase coexistence has been observed in the compositional range of 0.05 x 0.07. With increasing BZ content, their Tc and Toet values decrease gradually, and the dielectric constant increases linearly. The ceramic with x ¼ 0.06 exhibits an enhanced piezoelectric behavior (d33 w 193 pC/N and kp w 32.6%) because of the coexistence of two phases together with a dense microstructure. As a result,the construction of a rhombohedral and orthorhombic phase boundary is an effective way to improve the piezoelectric properties of KNN-based ceramics.

Changpingibacter yushuensis gen. nov., sp. nov., isolated from fluvial sediment in Qinghai Tibet Plateau of China

Jiao Yifan,Zhang Sihui,Yang Jing,Lai Xin-He,Dong Kui,Cheng Yanpeng,Xu Mingchao,Zhu Wentao,Lu Shan,Jin Dong,Pu Ji,Huang Ying,Liu Liyun,Wang Suping,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2

Two facultatively anaerobic, short rod-shaped, non-motile, Gram-stain-positive, unknown bacterial strains (JY-X040T and JY-X174) were isolated from fluvial sediments of Tongtian River in Yushu Tibetan Autonomous Prefecture, Qinghai province, China. Cells formed translucent, gray, round and convex colonies, with a diameter of less than 0.5 mm after 5 days of incubation at 30°C on brain heart infusion-5% sheep blood agar. The 16S rRNA gene sequence similarity between strain JY-X040T and Fudania jinshanensis 313T is 93.87%. In the four phylogenetic trees constructed based on the 16S rRNA gene and 423 core genes, the two isolates form an independent branch, phylogenetically closest to F. jinshanensis 313T, but could not be classified as a member of the genus Fudania or any other genus of the family Arcanobacteriaceae. The DNA G + C content of strain JY-X040T was 57.8%. Calculation results of average nucleotide identity, digital DNADNA hybridization value and amino acid identity between strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%, and 64.1%. The major cellular fatty acids were C16:0 (23%) and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α (L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and four unidentified components. The whole-cell sugars contained rhamnose and ribose. MK-10(H4) was the sole respiratory quinone. The minimum inhibitory concentration of streptomycin was 32 μg/ml. All physiological, biochemical, chemotaxonomic and genomic characteristics support that strains JY-X040T and JY-X174 represent members of a novel species in a new genus, Changpingibacter yushuensis gen. nov., sp. nov. The type strain is JY-X040T (GDMCC 1.1996T = KCTC 49514T).