Active Patchy Colloids with Shape-Tunable Dynamics

Wang, Zuochen,Wang, Zhisheng,Li, Jiahui,Cheung, Simon Tsz Hang,Tian, Changhao,Kim, Shin-Hyun,Yi, Gi-Ra,Ducrot, Etienne,Wang, Yufeng American Chemical Society 2019 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.141 No.37

<P>Controlling the complex dynamics of active colloids-the autonomous locomotion of colloidal particles and their spontaneous assembly-is challenging yet crucial for creating functional, out-of-equilibrium colloidal systems potentially useful for nano- and micromachines. Herein, by introducing the synthesis of active “patchy” colloids of various low-symmetry shapes, we demonstrate that the dynamics of such systems can be precisely tuned. The low-symmetry patchy colloids are made in bulk via a cluster-encapsulation-dewetting method. They carry essential information encoded in their shapes (particle geometry, number, size, and configurations of surface patches, etc.) that programs their locomotive and assembling behaviors. Under AC electric field, we show that the velocity of particle propulsion and the ability to brake and steer can be modulated by having two asymmetrical patches with various bending angles. The assembly of monopatch particles leads to the formation of dynamic and reconfigurable structures such as spinners and “cooperative swimmers” depending on the particle’s aspect ratios. A particle with two patches of different sizes allows for “directional bonding”, a concept popular in static assemblies but rare in dynamic ones. With the capability to make tunable and complex shapes, we anticipate the discovery of a diverse range of new dynamics and structures when other external stimuli (e.g., magnetic, optical, chemical, etc.) are employed and spark synergy with shapes.</P> [FIG OMISSION]</BR>

Yeast Culture and Vitamin E Supplementation Alleviates Heat Stress in Dairy Goats

Wang, Lizhi,Wang, Zhisheng,Zou, Huawei,Peng, Quanhui Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.6

This study was conducted to determine and compare the effects of yeast yeast culture (YC) and vitamin E (VE) supplementation on endotoxin absorption and antioxidant status in lactating dairy goats suffering from heat stress (HS). Three first lactation Saanen dairy goats (body weight $30{\pm}1.5kg$) were surgically fitted with indwelling catheters in the portal vein, mesenteric vein and carotid artery, and were randomly assigned to a $3{\times}3$ Latin square design. Dietary treatments were the basal diet, and the basal diet supplemented with either 100 IU VE or 30 g YC. Goats were kept in temperature and humidity-controlled room at $35^{\circ}C$ from 8:00 to 20:00 and at $24^{\circ}C$ from 20:00 till the next morning at 8:00. The relative humidity was kept at 55%. HS increased dairy goats' rectum temperature and respiration frequency (p<0.01). HS reduced plasma flux rate of milk goats (p<0.01), but the plasma flux rate increased when the animal was under the conditions of the thermo-neutral period (p<0.01). The VE supplementation lowered dairy goats' rectum temperature during thermo-neutral period (p<0.01). Meanwhile, no significant differences were observed between the control and YC treatment in rectum temperature and respiration frequency (p>0.05). Dietary supplementation of VE and YC reduced heat stressed dairy goats' endotoxin concentration of the carotid artery and portal vein (p<0.01). However, the endotoxin concentration of the YC treatment was higher than that of the VE treatment (p<0.01). Both VE and YC supplementation decreased heat stressed dairy goats' absorption of endotoxin in portal vein (p<0.01). The endotoxin absorption of YC treatment was higher than the VE treatment (p<0.01). The addition of VE and YC decreased dairy goats' superoxide dismutase (SOD) concentration during HS and the whole experiment period (p<0.01). The addition of VE lowered SOD concentration during thermo-neutral period (p<0.01). Likewise, the addition of VE and YC lowered dairy goats' malonaldehyde (MDA) concentration during HS and the whole experimental period, and the MDA concentration in the VE treatment was lower than the YC treatment (p<0.05). The addition of VE decreased MDA concentration during thermo-neutral period. On the contrast, the addition of VE increased dairy goats total antioxidant potential (TAP) concentration during HS, thermo-neutral and the whole experimental period (p<0.01). The addition of YC increased TAP concentration only during HS period (p<0.01). It is concluded that both VE and YC are useful in alleviating HS of dairy goats by weakening endotoxin absorption and promoting antioxidant capacity. Compared with YC, VE is much more powerful in easing dairy goats HS.

Research on the Control Strategy of Passive Torque Servo System for Loading at High Frequency

Zhisheng Ni,Mingyan Wang 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.6

iTRAQ-facilitated proteomic analysis of Bacillus cereus via degradation of malachite green

Bobo Wang,Jing Lu,Junfang Zheng,Zhisheng Yu 한국미생물학회 2021 The journal of microbiology Vol.59 No.2

The wide use of malachite green (MG) as a dye has caused substantial concern owing to its toxicity. Bacillus cereus can against the toxic effect of MG and efficiently decolourise it. However, detailed information regarding its underlying adaptation and degradation mechanisms based on proteomic data is scarce. In this study, the isobaric tags for relative and absolute quantitation (iTRAQ)-facilitated quantitative method was applied to analyse the molecular mechanisms by which B. cereus degrades MG. Based on this analysis, 209 upregulated proteins and 198 downregulated proteins were identified with a false discovery rate of 1% or less during MG biodegradation. Gene ontology and KEGG analysis determined that the differentially expressed proteins were enriched in metabolic processes, catalytic activity, antioxidant activity, and responses to stimuli. Furthermore, real-time qPCR was utilised to further confirm the regulated proteins involved in benzoate degradation. The proteins BCE_4076 (Acetyl-CoA acetyltransferase), BCE_5143 (Acetyl-CoA acetyltransferase), BCE_5144 (3-hydroxyacyl-CoA dehydrogenase), BCE_4651 (Enoyl-CoA hydratase), and BCE_5474 (3-hydroxyacyl-CoA dehydrogenase) involved in the benzoate degradation pathway may play an important role in the biodegradation of MG by B. cereus. The results of this study not only provide a comprehensive view of proteomic changes in B. cereus upon MG loading but also shed light on the mechanism underlying MG biodegradation by B. cereus.

Development of Plastic/Gelatin Bilayer Active Packaging Film with Antibacterial and Water-Absorbing Functions for Lamb Preservation

Shijing Wang,Weili Rao,Chengli Hou,Raheel Suleman,Zhisheng Zhang,Xiaoyu Chai,Hanxue Tian 한국축산식품학회 2023 한국축산식품학회지 Vol.43 No.6

In order to extend the shelf life of refrigerating raw lamb by inhibiting the growth of microorganisms, preventing the oxidation of fat and protein, and absorbing the juice outflow of lamb during storage, an active packaging system based on plastic/gelatin bilayer film with essential oil was developed in this study. Three kinds of petroleumderived plastic films, oriented polypropylene (OPP), polyethylene terephthalate, and polyethylene, were coated with gelatin to make bilayer films for lamb preservation. The results showed significant improvement in the mechanical properties, oxygen, moisture, and light barriers of the bilayer films compared to the gelatin film. The OPP/gelatin bilayer film was selected for further experiments because of its highest acceptance by panelists. If the amount of juice outflow was less than 350% of the mass of the gelatin layer, it was difficult for the gelatin film to separate from lamb. With the increase in essential oil concentration, the water absorption capacity decreased. The OPP/gelatin bilayer films with 20% mustard or 10% oregano essential oils inhibited the growth of bacteria in lamb and displayed better mechanical properties. Essential oil decreased the brightness and light transmittance of the bilayer films and made the film yellow. In conclusion, our results suggested that the active packaging system based on OPP/gelatin bilayer film was more suitable for raw lamb preservation than single-layer gelatin film or petroleum-derived plastic film, but need further study, including minimizing the amount of essential oil, enhancing the mechanical strength of the gelatin film after water absorption.

The Cloud Terminal Online Monitoring System of UPS Battery Performance based on MSP430 MCU

Yuan Zhisheng,Mingze Yuan,Haiying Wang,Tianjun Sun 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.6

Aiming at some problems in the process of UPS battery real-time monitoring, on the basis of the cloud terminal technology is proposed in this paper, a new method of remote online monitoring, and based on MSP430 single chip microcomputer, combined with modular guiding ideology, in order to improve the UPS battery remote monitoring automation as the core, through the reasonable design of hardware structure, the implementation of UPS battery on-line real-time monitoring of voltage, current and temperature. At the same time, the system based on ADO database access technology and C++ programming to complete the data management, human-computer interaction, and system control, by the cloud terminal implementation monitoring system data transmission between lower place machine and super-ordination machine. Practice has proved that the technology security and stability, has the very good practical and promotional value.

Facial Feature Extraction Based on Weighted ALW and Pulse-Coupled Neural Network

Junhua Liang,Zhisheng Zhao,Xiao Zhang,Xuan Wang,Yang Liu 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.8

In order to improve the robustness of face identification with the changes of illumina-tion, expression and facial alteration, a new facial feature extraction algorithm based on weighted adaptive lifting wavelet(ALW) scheme and pulse-coupled neural network (PCNN) is involved in this paper. The face images are decomposed into several subbands by weighted adaptive lifting scheme. Then the PCNN is utilized to decompose each weighted subbands into a series of binary images, the entropies of which are calculated and regarded as facial features. Experimental results show that the method yields a good robustness against the illumination, expression and facial variability and reduces the computer burden.

A Novel Spectral Matching LED Lighting Source for Plant

Fan Cao,Ailin Chen,Zhisheng Wang,Xiaoyang He,Nianyu Zou 한국조명·전기설비학회 2017 조명·전기설비학회논문지 Vol.31 No.8

This paper deals with the spectral matching optimization by a group of narrowband high power LEDs to achieve the target spectrum required by plant growing, the correlation index is a key evaluation criteria, higher value of which is often difficult to achieve because of the poor performance of spectral matching algorithm. The bat algorithm is proposed as a spectral matching algorithm. Parameters of bat algorithm under high correlation index are selected by observing the parameter variation tendency in the spectral matching simulation. In the experimental verification, 2 kinds of control circuit are used to match the target spectrum. Experimental results shows that appropriate parameter groups of bat algorithm must select based on their relationship with the correlation index. In practical experiment, the optimal correlation index is 0.70±0.05 under conditions of 3 selected bat algorithm parameter groups. It is of significance to plant lighting in the engineering practice exploratory.

Lactobacillus delbrueckii ssp. lactis R4 Prevents Salmonella typhimurium SL1344-Induced Damage to Tight Junctions and Adherens Junctions

Qinghua Yu,Liqi Zhu,Zhisheng Wang,Pengcheng Li,Qian Yang 한국미생물학회 2012 The journal of microbiology Vol.50 No.4

Cell junctions are the gatekeepers of the paracellular route and defend the mucosal barrier. Several enteropathogenic bacteria can invade intestinal epithelial cells by targeting and damaging cell junctions. It is not well understood how Salmonella typhimurium is able to overcome the intestinal barrier and gain access to the circulation, nor is it understood how Lactobacillus prevents the invasion of S. typhimurium. Therefore, we sought to determine whether infection with S. typhimurium SL1344 could regulate the molecular composition of cell junctions and whether Lactobacillus delbrueckii ssp. lactis R4 could affect this modification. Our data demonstrated that infection of Caco-2 cells with S. typhimurium over 2 h resulted in a redistribution of claudin-1, ZO-1, occluding,and E-cadherin. Western blot analysis of epithelial cell lysates demonstrated that S. typhimurium could decrease the expression of cell junction proteins. However, L. delbrueckii ssp. lactis R4 ameliorated this destruction and induced increased expression of ZO-1, occludin, and E-cadherin relative to the levels in the control group. The results of these experiments implied that S. typhimurium may facilitate its uptake and distribution within the host by regulating the molecular composition of cell junctions. Furthermore,Lactobacillus may prevent the adhesion and invasion of pathogenic bacteria by maintaining cell junctions and the mucosal barrier.

Online Fault Diagnosis in Discrete Event Systems with Partially Observed Petri Nets

Jiufu Liu,Zaihong Zhou,Zhisheng Wang 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.1

This paper investigates the fault detection problem for Discrete Event Systems (DES) which can be modeled by Partially Observed Petri Nets (POPN). To overcome the problem of low diagnosability in the POPN online fault diagnoser in current use, we propose an improved online fault diagnosis algorithm that integrates Generalized Mutual Exclusion Constraints (GMEC) and Integer Linear Programming (ILP).We assume that the POPN structure and its initial markings are known, and the faults are modeled as unobservable transitions. First, the event sequence is observed and recorded. We use GMEC for elementary diagnosis of the system behavior,then the ILP problem of POPN is solved for further diagnosis. Finally, we modeled and analyzed an example of a real DES to test the new fault diagnoser. The proposed algorithm increased the diagnosability of the DES remarkably, and the effectiveness of the new algorithm integrating GMEC and ILP was verified.