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- 저자 : Bobo Wang (검색결과 2 건)
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iTRAQ-facilitated proteomic analysis of Bacillus cereus via degradation of malachite green
Bobo Wang,Jing Lu,Junfang Zheng,Zhisheng Yu 한국미생물학회 2021 The journal of microbiology Vol.59 No.2
The wide use of malachite green (MG) as a dye has caused substantial concern owing to its toxicity. Bacillus cereus can against the toxic effect of MG and efficiently decolourise it. However, detailed information regarding its underlying adaptation and degradation mechanisms based on proteomic data is scarce. In this study, the isobaric tags for relative and absolute quantitation (iTRAQ)-facilitated quantitative method was applied to analyse the molecular mechanisms by which B. cereus degrades MG. Based on this analysis, 209 upregulated proteins and 198 downregulated proteins were identified with a false discovery rate of 1% or less during MG biodegradation. Gene ontology and KEGG analysis determined that the differentially expressed proteins were enriched in metabolic processes, catalytic activity, antioxidant activity, and responses to stimuli. Furthermore, real-time qPCR was utilised to further confirm the regulated proteins involved in benzoate degradation. The proteins BCE_4076 (Acetyl-CoA acetyltransferase), BCE_5143 (Acetyl-CoA acetyltransferase), BCE_5144 (3-hydroxyacyl-CoA dehydrogenase), BCE_4651 (Enoyl-CoA hydratase), and BCE_5474 (3-hydroxyacyl-CoA dehydrogenase) involved in the benzoate degradation pathway may play an important role in the biodegradation of MG by B. cereus. The results of this study not only provide a comprehensive view of proteomic changes in B. cereus upon MG loading but also shed light on the mechanism underlying MG biodegradation by B. cereus.
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Bobo Yang,Jiayue Xu,Jun Zou,Yan Zhang,Tian Tian,Yaoqing Chu,Meiling Wang 한양대학교 세라믹연구소 2016 Journal of Ceramic Processing Research Vol.17 No.6
Tm3+, Dy3+ and Tm3+/Dy3+ co-doped bismuth silicate (Bi4Si3O12, BSO) crystals were successfully grown by the modified verticalBridgeman method. The crystals have about 80% transmittance in the range from 320 nm to 650 nm except several obviouscharacteristic absorption peaks corresponding to transitions of 4f electrons of Tm3+ and Dy3+. The luminescence properties forwhite light emitting diode (w-LED) were investigated. Energy transfer from the Bi3+ ions to the Tm3+ and Dy3+ ions in Tm3+or/and Dy3+ co-doped Bi4Si3O12 crystal has been established by photoluminescence investigation upon UV excitation. Whenexcited by a proper UV-light, Tm3+ doped BSO crystal shows blue emission band centered at 460 nm ascribed to Tm3+ (1D2→ 3F4), Dy3+ doped BSO crystal shows blue band at 480 nm (4F9/2→ 6H15/2), yellow band at 574 nm (4F9/2→ 6H13/2) and red bandat 662 nm (4F9/2→ 6H11/2) of Dy3+ ions. A white light with chromaticity coordinate of x = 0.3298, y = 0.2905 by excitation of357 nm is achieved from Tm3+/Dy3+ co-doped Bi4Si3O12 crystal. These results indicate that Tm3+/Dy3+ co-doped Bi4Si3O12 as awhite emitting crystal has a potential application in white-LED.
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