Effects of Dietary Iodine and Selenium on the Activities of Blood Lymphocytes in Laying Hens

Song, Zhigang,Guo, Yuming,Yuan, Jianmin Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.5

The effect of dietary iodine and selenium supplementation, alone or in combination, on peripheral blood lymphocyte function was determined in laying hens. Eight-hundred-and-sixty-four New-Loman laying hens were randomly allotted into 12 dietary treatments with different inclusion levels of iodine (0, 0.1 and 0.2 mg/kg), selenium (0, 0.05, 0.1 and 0.2 mg/kg) or their combinations for 24 weeks. The lipopolysaccharide (LPS) stimulation index, concanavalin A (ConA) stimulation index, peroxide enzyme activity and phagocytosis to neutral red particles were tested. There were significant differences in LPS stimulation index, ConA stimulation index, peroxide enzyme activity and phagocytosis to neutral red particles in different iodine or selenium supplementation levels (p<0.05). The highest iodine and selenium supplementation both resulted in highest LPS-/ConA-stimulation indices (p<0.05). However, when iodine was lower than 0.2 mg/kg, the additional effect of different levels of selenium did not always result in significant differences in these indices. The results indicated that iodine and selenium may affect immunity in laying hens and, when the iodine level in the laying hen is lower than 0.2 mg/kg, a selenium allowance higher than 0.1 mg/kg may be necessary to improve immunity.

Effect of Corticosterone on Hypothalamic Corticotropin-releasing Hormone Expression in Broiler Chicks (Gallus gallus domesticus) Fed a High Energy Diet

Song, Zhigang,Yuan, Lei,Jiao, Hongchao,Lin, Hai Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.12

This paper reports the peripheral and central effect of corticosterone on feed intake and hypothalamic corticotropinreleasing hormone (CRH) gene expression in chicks fed a high energy diet. Three experiments were conducted: corticosterone was supplemented to the feed (30 mg/kg diet), injected subcutaneously (s.c., 4 mg/kg body weight) or intracerebroventricularly (i.c.v., 4 ng). The results showed that dietary corticosterone significantly increased feed intake. The s.c. corticosterone administration increased feed intake within 1 to 3 h and at 1 to 5 h after the injection. The i.c.v. corticosterone administration increased feed intake within 1 h after the injection, but not at 1 to 3 h. Dietary supplementation and s.c. injection of corticosterone decreased the CRH gene expression in the hypothalamus. Therefore, peripheral corticosterone exerted a decreased effect on hypothalamic CRH mRNA levels, and corticosterone had a stimulating effect on feed intake in broiler chicks fed a high energy diet.

Effects of intracerebroventricular injection of corticotrophin releasing factor on the gene expression of ghrelin and corticotrophin releasing factor receptors in broiler chickens

Cai, Yuanli,Song, Zhigang Asian Australasian Association of Animal Productio 2022 Animal Bioscience Vol.35 No.12

Objective: This study aimed to investigate the effects of corticotropin-releasing factor (CRF) on the feed intake of broiler chickens and explore its influencing mechanism. Methods: The study included two trials. In trial 1, 32 male broiler chickens (Arbor Acres, Gallus gallus domesticus) were given ventricle buried tubes, and they were allowed to recover for 3 days. At 8:00 AM, intracerebroventricular (ICV) injection with CRF or normal saline was performed in 10-day-old broiler chickens, which were divided into the 5, 10, and 20 ㎍ and control (normal saline) groups according to the dose of CRF injection. In trial 2, chickens were divided into the 10 ㎍ and control group (physiological saline) to repeat trial 1. Results: Results of trial 1 showed that the cumulative amount of feed intake in the 10 or 20 ㎍ groups was considerably lower than that of the control group after ICV injection with CRF. The lowest amount of feed intake was obtained with the addition of 10 ㎍ of CRF. In trial 2, the expression of ghrelin in the hypothalamus injected with 10 ㎍ of CRF increased significantly, but the expression of ghrelin in various sections of the small intestine considerably decreased. The expression of CRF receptor subtypes 1 (CRFR1) in the hypothalamus and some parts of the small intestine remarkably increased, and the expression of CRF receptor subtypes 2 (CRFR2) increased only in the duodenum, whereas the expression of growth hormone secretagogue receptor (GHSR-1α) in the jejunum and ileum increased considerably after ICV injection of 10 ㎍ of CRF. Conclusion: The CRF at 10 ㎍ increased ghrelin expression in the hypothalamus and CRFR1 expression in the small intestine, and this phenomenon was related to the suppressed feed intake of broiler chickens.

A Novel and Efficient Wireless Communication System

Wei Zhao,Yuehong Shen,Zhigang Yuan,Yimin Wei,Wei Jian 보안공학연구지원센터 2015 International Journal of Future Generation Communi Vol.8 No.5

This paper aims to construct a novel wireless communication system, in which source signals are transmitted simultaneously in the same frequency band. The transmitted signals are only required to be statistically independent or statistically distinguished. Therefore, the source signals can be recovered at the receiver by utilizing the classical algorithms of blind source separation (BSS) and independent component analysis (ICA) such as the fast fixed-point algorithm (FastICA). On the one hand, because the source signals are transmitted simultaneously in the same frequency band, the spectrum efficiency of this novel system is much higher than those of time division multiplexing (TDM), frequency division multiplexing (FDM), and code division multiplexing (CDM) systems, in which TDM, FDM and CDM signals are limited in time interval, frequency band and code. On the other hand, inspired by recently proposed reference-based schemes, the reference signals are introduced to the classical separation algorithms of BSS and ICA, which makes this novel system much more efficient than classical ones in terms of computational speed. The performance of this new system is validated through realistic experiments. Additionally, it is theoretically shown that the information content of all the source signal inputs can be recovered by this novel wireless communication system.

Experimental Study on Key Parameters of Bidirectional Cumulative Tensile Blasting with Coal-Containing Composite Roof

Pengfei Guo,Kengkeng Ye,Zhigang Tao,Hongda Liang,Yadi Yuan 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5

To improve the effectiveness of the directional-predetermined crack blasting in the weak and alternate composite roof, this paper took the coal-containing composite roof of the 12201 working face of Halagou Coal Mine as the engineering background, and based on the technical principles of roof cutting pressure releasing gob-side entry retaining, comprehensive used of theoretical analysis, field experiments and other means, carried out in-depth research on the shaped energy blasting method and parameter design of the coal-containing composite roof. The results show: Through theoretical analysis, in the process of shaped energy blasting, the air column in the hole can reduce the peak blast pressure, which can effectively prevent the weak surrounding rock in the hole from being damaged in the non-cumulative direction and enhance the pre-splitting effect. During detonation at the bottom, the instantaneous pressure in the blast hole is lower than that of detonation at the top, and the action time of blast pressure is longer than detonation at the top. When the buried depth of the explosive (near the blast hole) is less than the critical depth, a "V"-shaped blasting funnel will be formed after the rock blasting, and the critical depth is proportional to the charge. Through field tests on the charge quantity and the length of the sealing mud, when the blast hole depth is 6m, the charge parameters of "3+2+0+1" have a better effect of energy-concentrating blasting, and the crack rate reaches 87%. Besides, field tests have shown that when shaped energy blasting is carried out on soft and hard rock formations, the stemming separation in the shaped energy tube at the interlayer position can ensure the directional pre-cracking effect of hard rock, while effectively avoiding damage to the weak rock formation in the non-cumulative direction.

Rapid Detection of Food-borne Listeria monocytogenes by Real-time Quantitative Loop-mediated Isothermal Amplification

Xiaoxiao Shan,Yaoqi Zhang,Zhigang Zhang,Miaorui Chen,Yongyu Su,Yingna Yuan,M. Jahangir Alam,He Yan,Lei Shi 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.1

The purpose of this study was to develop a real-time quantitative loop-mediated isothermal amplication (LAMP) method for the rapid, sensitive, and convenient detection of Listeria monocytogenes in food. The LAMP method could amplify the hlyA gene of L. monocytogenes successfully at 63oC with a loopamp real-time turbidimeter. The detection limits of the LAMP for hlyA gene were 6colony forming units (CFU)/tube. A standard curve was generated for L. monocytogenes LAMP by plotting the graph based different log CFU values of L. monocytogenes and time of positivity through real-time monitoring of the amplication. Then, the LAMP method was employed to test 94 retail food samples effectively. Sensitivity in detection of L. monocytogenes by the LAMP was higher than that of PCR and none of the conventional methodpositive samples was missed by the LAMP method.

Prevalence of Decreased Myocardial Blood Flow in Symptomatic Patients with Patent Coronary Stents: Insights from Low-Dose Dynamic CT Myocardial Perfusion Imaging

Yuehua Li,Mingyuan Yuan,Mengmeng Yu,Zhigang Lu,Chengxing Shen,Yining Wang,Bin Lu,Jiayin Zhang 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.4

Objective: To study the prevalence and clinical characteristics of decreased myocardial blood flow (MBF) quantified by dynamic computed tomography (CT) myocardial perfusion imaging (MPI) in symptomatic patients without in-stent restenosis. Materials and Methods: Thirty-seven (mean age, 71.3 ± 10 years; age range, 48–88 years; 31 males, 6 females) consecutive symptomatic patients with patent coronary stents and without obstructive de novo lesions were prospectively enrolled to undergo dynamic CT-MPI using a third-generation dual-source CT scanner. The shuttle-mode acquisition technique was used to image the complete left ventricle. A bolus of contrast media (50 mL; iopromide, 370 mg iodine/mL) was injected into the antecubital vein at a rate of 6 mL/s, followed by a 40-mL saline flush. The mean MBF value and other quantitative parameters were measured for each segment of both stented-vessel territories and reference territories. The MBFratio was defined as the ratio of the mean MBF value of the whole stent-vessel territory to that of the whole reference territory. An MBFratio of 0.85 was used as the cut-off value to distinguish hypoperfused from non-hypoperfused segments. Results: A total of 629 segments of 37 patients were ultimately included for analysis. The mean effective dose of dynamic CT-MPI was 3.1 ± 1.2 mSv (range, 1.7–6.3 mSv). The mean MBF of stent-vessel territories was decreased in 19 lesions and 81 segments. Compared to stent-vessel territories without hypoperfusion, the mean MBF and myocardial blood volume were markedly lower in hypoperfused stent-vessel territories (77.5 ± 16.6 mL/100 mL/min vs. 140.4 ± 24.1 mL/100 mL/min [p < 0.001] and 6.4 ± 3.7 mL/100 mL vs. 11.5 ± 4 mL/100 mL [p < 0.001, respectively]). Myocardial hypoperfusion in stentvessel territories was present in 48.6% (18/37) of patients. None of clinical parameters differed statistically significantly between hypoperfusion and non-hypoperfusion subgroups. Conclusion: Decreased MBF is commonly present in patients who are symptomatic after percutaneous coronary intervention, despite patent stents and can be detected by dynamic CT-MPI using a low radiation dose.

Study on Multi Objective Optimization Method and It Application in Aviation and Deceleration Device

Yu Guang-bin,Song Ye,Duan Yuan-wang,Zhao Xing-fu,Qu Zhigang,Sun Yong-guo 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.5

A Novel Wireless Statistical Division Multiplexing Communication System and Performance Analysis

Wei Zhao,Yuehong Shen,Pengcheng Xu,Jiangong Wang,Zhigang Yuan,Yimin Wei,Wei Jian,Hui Li 보안공학연구지원센터 2014 International Journal of Future Generation Communi Vol.7 No.5

A New Ambiguity Elimination Method for BSS Block Signals in Time Domain

Wei Zhao,Fengshan Wang,Yuehong Shen,Yuanyuan Wu,Zhigang Yuan,Pengcheng Xu,Pengcheng Xu,Yimin Wei,Wei Jian 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.11

This paper deals with the ambiguity problem of blind source separation (BSS) in the case where continuously received mixture signals are split in time and processed block by block. Due to the inherent permutation and scaling ambiguities of BSS, tying the separated components at each adjacent time blocks doesn’t recover the original source signals correctly in general. Inspired by the Permutation Method of reconstructing source signal blocks in time domain, a new ambiguity elimination approach is proposed in this paper. This method aims to concatenate the separated components in adjacent blocks by artificially setting contrast blocks for each adjacent time blocks. The core idea of this method is to utilize the associativity between components recovered from contrast blocks and corresponding adjacent blocks. Compared with Permutation Method, the main advantage of this new method consists in the fact that it is much more efficient in terms of separation quality and computational speed. Besides, a tradeoff can be adjusted between separation quality and computational speed by choosing different length of contrast blocks. Real-life experiments are performed to validate the performance of this method on the wireless communication system with two transmitting and receiving antennas.