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- 저자 : Xiaoxiao Shan (검색결과 2 건)
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Xiaoxiao Shan,Yaoqi Zhang,Zhigang Zhang,Miaorui Chen,Yongyu Su,Yingna Yuan,M. Jahangir Alam,He Yan,Lei Shi 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.1
The purpose of this study was to develop a real-time quantitative loop-mediated isothermal amplication (LAMP) method for the rapid, sensitive, and convenient detection of Listeria monocytogenes in food. The LAMP method could amplify the hlyA gene of L. monocytogenes successfully at 63oC with a loopamp real-time turbidimeter. The detection limits of the LAMP for hlyA gene were 6colony forming units (CFU)/tube. A standard curve was generated for L. monocytogenes LAMP by plotting the graph based different log CFU values of L. monocytogenes and time of positivity through real-time monitoring of the amplication. Then, the LAMP method was employed to test 94 retail food samples effectively. Sensitivity in detection of L. monocytogenes by the LAMP was higher than that of PCR and none of the conventional methodpositive samples was missed by the LAMP method.
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Effect of HPAM on Calcium Carbonate Crystallization
Jing, Guolin,Tang, Shan,Li, Xiaoxiao Korean Chemical Society 2013 대한화학회지 Vol.57 No.3
With the wide application of ASP (alkaline-surfactant-polymer) flooding, the scaling becomes more and more serious, which is harmful to the oilfield and environment. In order to investigate the effects of HPAM on calcium carbonate crystallization, the crystallization behaviors of $CaCO_3$ in HPAM (Hydrolyzed polyacrylamide) solutions were studied and the composition and morphology of $CaCO_3$ crystal were investigated in different concentrations of polyacrylamide solutions. The crystal forms and morphologies of $CaCO_3$ were characterized by Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. The results show that the crystallization of $CaCO_3$ is strongly influenced by the HPAM. The paper analyzed the internal cause, and the results show: The reasons leading to the change of morphology are carboxyl groups in polyacrylamide molecule and $Ca^{2+}$ in solution form chelates by coordination bond. And the chelates are adsorbed on the calcium hydroxide surfaces of solid-liquid interfaces so as to change the formation rate of calcium carbonate crystal nucleus. The research provides a reliable basis for the mechanism research of the scaling problem in the oil extraction process of ASP flooding and the adoption of scale inhibition and scale inhibitor.
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