Transcriptome profile of Chinese bush cricket, Gampsocleis gratiosa: A resource for microsatellite marker development

Zhi‐Jun Zhou,Xiao-Yan KOU,Lei-YangQIAN,Jing LIU 한국곤충학회 2016 Entomological Research Vol.46 No.3

The Chinese bush cricket, Gampsocleis gratiosa, has a long history as a pet in China. To date, the sequencing of its whole genome is unavailable as a non‐model organism. Transcriptomic information is also scarce for this species. The G. gratiosa transcriptome was sequenced using Illumina HiSeq 2000 paired‐end sequencing technology. In total 52 million clean reads with an average length of 90 bp were generated, which produced 74,821 unigenes with a mean length of 580 bp and an N50 length of 759 bp. In total 29,674 (39.66%) unigenes were successfully annotated against the NR, NT, Swiss‐Prot, Gene Ontology (GO), Clusters of Orthologous Groups of proteins (COGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Further functionally classified of unigenes against GO, COGs and KEGG found that a total of 11,935 (19.95%) unigenes were categorized into 61 GO terms, 19,576 unigenes were clustered into 25 COG functional categories and 17,971 unigenes were assigned to 258 KEGG pathways. In addition, 2093 microsatellite loci were identified, of which 591 loci had flanking sequences suitable for polymerase chain reaction (PCR) primer design. The transcriptome profile of G. gratiosa contributed to the accumulation of orthopteran genomic data, and the microsatellite loci provided useful tools for future studies of this and other closely related Gampsocleis species.

Simultaneous Information and Power Transfer for Multi-antenna Primary-Secondary Cooperation in Cognitive Radio Networks

Zhi Hui Liu,Wen Jun Xu,Sheng Yu Li,Cheng Zhi Long,Jia Ru Lin 한국전자통신연구원 2016 ETRI Journal Vol.38 No.5

In this paper, cognitive radio and simultaneous wireless information and power transfer (SWIPT) are effectively combined to design a spectrum-efficient and energy-efficient transmission paradigm. Specifically, a novel SWIPT-based primary-secondary cooperation model is proposed to increase the transmission rate of energy/spectrum constrained users. In the proposed model, a multi-antenna secondary user conducts simultaneous energy harvesting and information forwarding by means of power splitting (PS), and tries to maximize its own transmission rate under the premise of successfully assisting the data delivery of the primary user. After the problem formulation, joint power splitting and beamforming optimization algorithms for decode-and-forward and amplify-and-forward modes are presented, in which we obtain the optimal PS factor and beamforming vectors using a golden search method and dual methods. Simulation results show that the proposed SWIPTbased primary-secondary cooperation schemes can obtain a much higher level of performance than that of non-SWIPT cooperation and non-cooperation schemes.

5-Aza-2'-deoxycytidine Induces Hepatoma Cell Apoptosis via Enhancing Methionine Adenosyltransferase 1A Expression and Inducing S-Adenosylmethionine Production

Liu, Wei-Jun,Ren, Jian-Guo,Li, Ting,Yu, Guo-Zheng,Zhang, Jin,Li, Chang-Sheng,Liu, Zhi-Su,Liu, Quan-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

In hepatocellular cancer (HCC), lack of response to chemotherapy and radiation treatment can be caused by a loss of epigenetic modifications of cancer cells. Methionine adenosyltransferase 1A is inactivated in HCC and may be stimulated by an epigenetic change involving promoter hypermethylation. Therefore, drugs releasing epigenetic repression have been proposed to reverse this process. We studied the effect of the demethylating reagent 5-aza-2'-deoxycitidine (5-Aza-CdR) on MAT1A gene expression, DNA methylation and S-adenosylmethionine (SAMe) production in the HCC cell line Huh7. We found that MAT1A mRNA and protein expression were activated in Huh7 cells with the treatment of 5-Aza-CdR; the status of promoter hypermethylation was reversed. At the same time, MAT2A mRNA and protein expression was significantly reduced in Huh7 cells treated with 5-Aza-CdR, while SAMe production was significantly induced. However, 5-Aza-CdR showed no effects on MAT2A methylation. Furthermore, 5-Aza-CdR inhibited the growth of Huh7 cells and induced apoptosis and through down-regulation of Bcl-2, up-regulation of Bax and caspase-3. Our observations suggest that 5-Aza-CdR exerts its anti-tumor effects in Huh7 cells through an epigenetic change involving increased expression of the methionine adenosyltransferase 1A gene and induction of S-adenosylmethionine production.

Preparation of Flame Retardant and Antibacterial Wood with Composite Membrane Coating

( Jun-xian Xu ),( Yang Liu ),( Ming-yu Wen ),( Hee-jun Park ),( Jia-zhi Zhu ),( Yu-nan Liu ) 한국목재공학회 2021 목재공학 Vol.49 No.6

A novel flame retardant and antibacterial composite membrane coating for wood surfaces was prepared by adding POSS-based phosphorous nitrogen flame retardant (later referred to as NH₂-POSS) and silver nanoparticles (Ag NPs) to chitosan (CS). The effects of NH₂-POSS content (mass fractions of CS 0%, 0.5%, 1%, 3%, 5%, and 7%) on the structure and properties of the composite membrane coating on wood were investigated. The composite film was prepared by the method of blending and ducting. Contact angle, tensile property and antibacterial effects of the composite film were measured, and infrared spectroscopy was used. The results show that the addition of NH₂-POSS can not only improve the toughness of the membrane, but also the flame retardancy of the membrane, which improves the application of the membrane in wood products. However, with the addition of NH₂-POSS, the transparency of the composite membrane was weakened. The inhibitory effect of the composite membrane on the growth of Escherichia coli was enhanced with the increase in Ag NPs. This research provides a foundation for the application of functional wood.

A Study on the Strain Stability of Si/SiGe Layer Structure in a Heterojunction Bipolar Transistor during Thermal Processing

Zhi Hong Liu,Chang Chun Chen,Wen Tao Huang,Wei Zhi Dou,Yi Lin Shan,Wei Zhang,Jun Zhu,Pei Hsin Tsien 대한금속학회 2004 METALS AND MATERIALS International Vol.10 No.3

Electroacupuncture Improves Insulin Resistance by Reducing Neuroprotein Y/ Agouti-Related Protein Levels and Inhibiting Expression of Protein Tyrosine Phosphatase 1B in Diet-induced Obese Rats

Xia Liu,Jun-Feng He,Ya-Ting Qu,Zhi-Jun Liu,Qing-Yang Pu,Shengtong Guo,Jia Du,Peng-Fei Jiang 사단법인약침학회 2016 Journal of Acupuncture & Meridian Studies Vol.9 No.2

Electroacupuncture (EA) has been shown to exert beneficial effects on obesity, but the mechanism is unclear. This study investigated the effects of EA on diet-induced obese (DIO) rats. Fifty male SpragueeDawley rats were randomly divided into low-fat diet (LFD, 10 rats) and high-fat diet (HFD, 40 rats) groups. After the DIO models had been established, successful model rats were randomly divided into HFD, EA, and orlistat (OLST) groups. The EA group received EA at Zusanli (ST36) and Quchi (LI11) for 20 minutes once per day for 28 days. The OLST group was treated with orlistat by gavage. The body weight, homeostasis model assessment-insulin resistance index, adipocyte diameters, and neuroprotein Y/agouti-related protein and protein tyrosine phosphatase 1B levels were significantly lower in the EA group than in the HFD group. The rats of the OLST group showed watery stools and yellow hairs whereas those of the EA group had regular stools and sleek coats. The effect of EA on weight loss may be related to improved insulin resistance caused by changes in the adipocyte size and by reductions in the expressions of neuroprotein Y/agouti-related protein and protein tyrosine phosphatase 1B. This study indicates that EA may be a better method of alternative therapy for treating obesity and other metabolic diseases.

Research on Flame Retardant Plywood with Different Flame Retardants

( Yang Liu ),( Jun-xian Xu ),( Ming-yu Wen ),( Hee-jun Park ),( Jia-zhi Zhu ),( Yu-nan Liu ) 한국목재공학회 2021 목재공학 Vol.49 No.6

The flame retardancy of plywood should be improved as much as possible while minimizing the impact on the bonding strength of plywood. Six commercial flame retardants and three laboratory synthesized phosphorous nitrogen flame retardants were selected. E₀, E₁ and E₂ grade commercial formaldehyde resins (UF) were applied in this study to evaluate the effect of different flame retardants on the curing time of resin, bonding strength, flame retardant performance, and formaldehyde emission of plywood. The results show that the effect of the addition of different flame retardants on the bonding strength of plywood gradually decreased with the increase of the formaldehyde molar ratio of the resin. The effect of flame retardants on the curing time of UF gradually decreased as the mole ratio of formaldehyde increasing, while the amount of formaldehyde emission varied according to the content of formaldehyde in the flame retardant. Compared with plywood without flame retardant, flame retardant of plywood added with phosphorous nitrogen flame retardant was improved.

Macrophages Promote Coal Tar Pitch Extract-induced Tumorigenesis of BEAS-2B Cells and Tumor Metastasis in Nude Mice Mediated by AP-1

Zhang, Peng,Jin, Yue-Fei,Zhang, Qiao,Wu, Yi-Ming,Wu, Wei-Dong,Yao, Wu,Wu, Yong-Jun,Li, Zhi-Tao,Zhao, Yong,Liu, Yu,Feng, Fei-Fei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: We sought to evaluate the role of tumor associated macrophages (TAMs) on the promotion of coal tar pitch extract (CTPE)-induced tumorigenesis of human bronchial epithelial cells (BEAS-2B) and tumor metastasis in nude mice, and related mechanisms. Materials and Methods: BEAS-2B cells were first treated with 2.4 mg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured and passaged using trypsin-EDTA. THP-1 cells were used as macrophage-like cells. BEAS-2B cells under different conditions (n=6/group) were injected into the back necks of nude mice, and alterations of tumor xenograft growth, indicative of tumorigenicity, and tumor metastasis were determined. Pathological changes (tumor nests and microvascular lesions) of HE-stained tumor tissues were also evaluated. The expression of AP-1(c-Jun) in xenografts and metastatic tumors was determined using immunohistochemistry. Results: Tumor size and weight in nude mice transplanted with the mixture of CTPE-induced passage 30 BEAS-2B and THP-1 cells (2:1) were increased compared to those from the CTPE-treated BEAS-2B cells at passage 30 alone at different observation time points. Tumor metastasis to lymph nodes and liver was only detected after transplantation of a mixture the two kinds of cells. The numbers of tumor nests and microvascular lesions, and the expression levels of AP-1 (c-Jun) in tumors from the mixture of two kinds of cells were increased apparently in contrast to those in tumor from the CTPE-treated BEAS-2B cells of passage 30 alone. In addition, there was positive correlation between AP-1 (c-Jun) expression level and the number of microvascular lesions, or between AP-1 (c-Jun) expression level and tumor metastasis in these two groups. Conclusions: TAMs not only facilitate tumorigenesis transformation of CTPE-induced BEAS-2B cells, but also promote tumor growth, angiogenesis and metastasis in nude mice in vivo, which may be mediated by AP-1.

Serum Retinol-Binding Protein Levels Are Associated with Nonalcoholic Fatty Liver Disease in Chinese Patients with Type 2 Diabetes Mellitus: A Real-World Study

Zhi-Hui Zhang,Jiang-Feng Ke,Jun-Xi Lu,Yun Liu,Ai-Ping Wang,Lian-Xi Li 대한당뇨병학회 2022 Diabetes and Metabolism Journal Vol.46 No.1

Background: The association of serum retinol-binding protein (RBP) levels with nonalcoholic fatty liver disease (NAFLD) remains controversial. Furthermore, few studies have investigated their relationship in type 2 diabetes mellitus (T2DM) patients. Therefore, the aim of the present study was to explore the association between serum RBP levels and NAFLD in Chinese inpatients with T2DM.Methods: This cross-sectional, real-world study included 2,263 Chinese T2DM inpatients. NAFLD was diagnosed by abdominal ultrasonography. The subjects were divided into four groups based on RBP quartiles, and clinical characteristics were compared among the four groups. The associations of both RBP levels and quartiles with the presence of NAFLD were also analyzed.Results: After adjustment for sex, age, and diabetes duration, there was a significant increase in the prevalence of NAFLD from the lowest to the highest RBP quartiles (30.4%, 40.0%, 42.4%, and 44.7% for the first, second, third, and fourth quartiles, respectively, P<0.001 for trend). Fully adjusted multiple logistic regression analysis revealed that both increased RBP levels (odds ratio, 1.155; 95% confidence interval, 1.012 to 1.318; P=0.033) and quartiles (P=0.014 for trend) were independently associated with the presence of NAFLD in T2DM patients.Conclusion: Increased serum RBP levels were independently associated with the presence of NAFLD in Chinese T2DM inpatients. Serum RBP levels may be used as one of the indicators to assess the risk of NAFLD in T2DM patients.

Actinorugispora endophytica gen. nov., sp. nov., an actinomycete isolated from Daucus carota.

Liu, Min-Jiao,Zhu, Wen-Yong,Li, Jie,Zhao, Guo-Zhen,Xiong, Zhi,Park, Dong-Jin,Hozzein, Wael N,Kim, Chang-Jin,Li, Wen-Jun Society for General Microbiology 2015 International journal of systematic and evolutiona Vol.65 No.8

<P>An actinomycete strain, designated YIM 690008T, was isolated from Daucus carota collected from South Korea and its taxonomic position was investigated by using a polyphasic approach. The strain grew well on most media tested and no diffusible pigment was produced. The aerial mycelium formed wrinkled single spores and short spore chains, some of which were branched. The whole-cell hydrolysates contained meso-diaminopimelic acid, glucose, mannose, ribose, galactose and rhamnose. The predominant menaquinones were MK-10(H4), MK-10(H6), MK-10(H8) and MK-10(H2). The polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannosides, some unknown phospholipids, glycolipids and polar lipids. The major fatty acids were i-C16?:?0, ai-C17?:?0 and C18?:?1ω9c. The DNA G+C content of the genomic DNA was 63.1?mol%. Phylogenetic analysis indicated that the isolate belongs to the family Nocardiopsaceae. However, based on phenotypic, chemotaxonomic and genotypic data, it was concluded that strain YIM 690008T represents a novel genus and novel species of the family Nocardiopsaceae, for which the name Actinorugispora endophytica gen. nov., sp. nov. (type strain YIM 690008T?=?DSM 46770T?=?JCM 30099T?=?KCTC 29480T) is proposed.</P>