Enhancement of transglutaminase production in Streptomyces mobaraensis DSM 40587 by non-nutritional stress conditions: Effects of heat shock, alcohols, and salt treatments

Lili Zhang,Lanwei Zhang,Huaxi Yi,Ming Du,Yingchun Zhang,Xue Han,Zhen Feng,Jingyan Li,Yuehua Jiao,Yanhe Zhang,Chunfeng Guo 한국화학공학회 2012 Korean Journal of Chemical Engineering Vol.29 No.7

Stress-mediated bioprocess is a strategy designed to enhance biological target productivity. In this study,an attempt was made to enhance transglutaminase (TGase) production by Streptomyces mobaraensis by using different stress conditions including heat shock, alcohols and salt stress. Results showed that the effects of stress on TGase production depended on the type applied. For heat shock, TGase production (1.32 U/ml) was recorded maximum in the culture treated at 48 h post inoculation in water bath at 60 oC for 1 min. For alcohols treatment, the maximum activity of TGase (1.77 and 1.75 U/ml) was obtained when 3% methanol was added into the medium at 0 or 24 h of fermentation. However, a 3.5-fold increased production of TGase (3.8 U/ml) was observed in the medium supplemented with 0.2mol/l MgCl2 compared with the basic medium at the beginning of fermentation. In conclusion, TGase production from S. mobaraensis was improved by heat shock, methanol and salt stress treatments, MgCl2 stress was the most effective.

MiR-26a promotes apoptosis of porcine granulosa cells by targeting the 3β-hydroxysteroid-Δ24-reductase gene

Zhang, Xiaodong,Tao, Qiangqiang,Shang, Jinnan,Xu, Yiliang,Zhang, Liang,Ma, Yingchun,Zhu, Weihua,Yang, Min,Ding, Yueyun,Yin, Zongjun Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.4

Objective: Apoptosis of ovarian granulosa cells (GCs) affects mammalian follicular development and fecundity. This study aimed to explore the regulatory relationship between microRNA-26a (miR-26a) and the 3β-hydroxysteroid-Δ24-reductase gene (DHCR24) gene in porcine follicular granular cells (pGCs), and to provide empirical data for the development of methods to improve the reproductive capacity of pigs. Methods: The pGCs were transfected with miR-26a mimic, miR-26a inhibitor and DHCR24-siRNA in vitro. The cell apoptosis rate of pGCs was detected by the flow cytometry. The secretion levels of estradiol (E2) and progesterone (P) in pGCs were detected by enzyme-linked immunosorbent assay. Double luciferase validation system was used to detect the binding sites between miR-26a and DHCR24 3'-UTR region. Qualitative real-time polymerase chain reaction and Western blotting were used to verify the DHCR24 mRNA and protein expression in pGCs, respectively, after transfecting with miR-26a mimic and miR-26a inhibitor. Results: Results showed that enhancement of miR-26a promoted apoptosis, and inhibited E2 and P secretion in pGCs. Meanwhile, inhibition of DHCR24 also upregulated the Caspase-3 expression, reduced the BCL-2 expression, promoted pGCs apoptosis, and inhibited E2 and P secretion in pGCs. There were the binding sites of miR-26a located within DHCR24 3'-UTR. Up-regulation of miR-26a inhibited DHCR24 mRNA and protein expression in pGCs. Conclusion: This study demonstrates that miR-26a can promote cell apoptosis and inhibit E2 and P secretion by inhibiting the expression of DHCR24 in pGCs.

Synergistic Activities of Abdominal Muscles Are Required for Efficient Micturition in Anesthetized Female Mice

Chuan Zhang,Yingchun Zhang,Yolanda Cruz,Timothy B. Boone,Alvaro Munoz 대한배뇨장애요실금학회 2018 International Neurourology Journal Vol.22 No.1

Purpose: To characterize the electromyographic activity of abdominal striated muscles during micturition in urethane-anesthetized female mice, and to quantitatively evaluate the contribution of abdominal responses to efficient voiding. Methods: Cystometric and multichannel electromyographic recordings were integrated to enable a comprehensive evaluation during micturition in urethane-anesthetized female mice. Four major abdominal muscle domains were evaluated: the external oblique, internal oblique, and superior and inferior rectus abdominis. To further characterize the functionality of the abdominal muscles, pancuronium bromide (25 μg/mL or 50 μg/mL, abdominal surface) was applied as a blocking agent of neuromuscular junctions. Results: We observed a robust activation of the abdominal muscles during voiding, with a consistent onset/offset concomitant with the bladder pressure threshold. Pancuronium was effective, in a dose-dependent fashion, for partial and complete blockage of abdominal activity. Electromyographic discharges during voiding were significantly inhibited by applying pancuronium. Decreased cystometric parameters were recorded, including the peak pressure, pressure threshold, intercontractile interval, and voiding duration, suggesting that the voiding efficiency was significantly compromised by abdominal muscle relaxation. Conclusions: The relevance of the abdominal striated musculature for micturition has remained a topic of debate in human physiology. Although the study was performed on anesthetized mice, these results support the existence of synergistic abdominal electromyographic activity facilitating voiding in anesthetized mice. Further, our study presents a rodent model that can be used for future investigations into micturition-related abdominal activity.

Voiding Dysfunction in Old Male Rats Associated With Enlarged Prostate and Irregular Afferent-Triggered Reflex Responses

Chuan Zhang,Xuhong Li,Timothy B. Boone,Yolanda Cruz,Yingchun Zhang,Alvaro Munoz 대한배뇨장애요실금학회 2020 International Neurourology Journal Vol.24 No.3

Purpose: This study was conducted to evaluate the hypothesis that an enlarged prostate in old rats may lead to complications associated with voiding dysfunction involving ionotropic P2X2/3-type purinergic receptors Methods: Intact animals were divided into male young (MYR; 8–10 weeks old) and male old (MOR; 20 months old) rats. The animals underwent simultaneous detrusor electromyography (EMG) and suprapubic cystometry (CMG) under urethane anesthesia. Immunofluorescence techniques were used to evaluate prostatic autonomic innervation and P2X3R expression in bladder urothelial cells. The functional role of P2X3R was characterized by intramuscular application of AF-353, a selective P2X2/3R antagonist. Results: The prostate index significantly increased in MOR, suggestive of an enlarged prostate affecting micturition patterns. Significant EMG and CMG differences were found between MYR and MOR. Higher immunoreactivity for P2X2/3R in the urothelial layer and for prostatic neurofilaments was seen in MOR. Systemic inhibition of P2X2/3R had minimal effects on MYR responsiveness, but improved voiding function in MOR with a marked decrease of intravesical pressure and bladder contractile responses. Conclusions: The data support the hypothesis that an enlarged prostate in MOR may contribute to voiding dysfunction involving activation of P2X2/3R, which enhances a prostate-bladder reflex. This reflex may increase bladder afferent transmission and activation of increased prostate innervation, leading to voiding dysfunction.

MnFe2O4 nanoparticles coated on one-dimensional carbon nanowires derived from Nitrilotriacetic acid as efficient catalysts to activate peroxymonosulfate for moxifloxacin degradation

Xuefeng Zhang,Yujie Zhang,Qin Yang,Yingchun Yang,Xuping Sun 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.127 No.-

Sulfate radical-based advanced oxidation processes (AOPs) are highly reliable for the elimination of recalcitrantcontaminants by increasing degradability and reducing toxicity. Here, MnFe2O4 nanowires(MnFe2O4 NWs), composed of abundant MnFe2O4 nanoparticles immobilized on one-dimensional carbonnanowires derived from annealed MnFe-Nitrilotriacetic acid (MnFe-NTA) precursor was successfully synthesized. The MnFe2O4 NWs, which could provide more active sites, were then utilized to activate peroxymonosulfate(PMS) for oxidizing the target pollutant Moxifloxacin (MOX) in an aqueous solution. TheMnFe2O4 NWs/PMS system acquired 91.9% removal of MOX and achieved 55.1% chemical oxygen demand(COD) degradation efficiency in 30 min. The results exhibited that the increased catalyst doses and PMSconcentration lead to ascending MOX removal rate, which decreased with the participation of co-existingions. Besides, there is a close relationship between original pH and MOX degradation efficiency. It wasfound that SO4 –, OH, 1O2, and O2 – were involved in the MOX degradation by quenching experimentsand electron paramagnetic resonance (EPR) detection. More importantly, the stable magnetism ofMnFe2O4 NWs contributed to its convenient recycling. Finally, a reliable mechanism for activating PMSwas proposed based on the aforementioned results and previous researches, which could exhibit a novelhorizon in effluent treatment.

Simulation Analysis on Hysteretic Performance of Cross-type Rigid Node for Steel Frame Based on Abaqus

Jing Ji,Bing Li,Wenfu Zhang,Yingchun Liu,Chaoqing Yuan,Fei Xing 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.1

In order to study the cross-type rigid steel frame node hysteretic behavior of components, the axial compression ratio, H type steel column web thickness, girder section and stiffener as design parameters, 8 steel frame cross-type rigid joints and a T-type rigid joint are designed. Based on simplified mechanical model and constitutive relations of material, using ABAQUS software the corresponding finite element model is established and the hysteretic performance analysis is carried out. Through comparison between given T-type node test results and analysis results, both are in good agreement, thus the rationality of the finite element model established in this paper is verified. Simulation analysis of cross-type node is developed in further, load -displacement hysteretic curve of nodes, skeleton curve, envelope figure and the stress nephogram are extracted and comparative analysis is conducted, influence regularity of four parameters on the seismic behavior of the joints is obtained. The corresponding design suggestion for the axial compression ratio and column web and flange thickness ratio is proposed, design limit value of axial compression ratio of column should be taken as 0.5 and limit value of column web and flange thickness ratio is taken as 0.75,and these can provide reference for actual engineering design.

Sensory, Physicochemical, and Microbiological Changes in Vacuum Packed Channel Catfish (Clarias lazera) Patties during Controlled Freezing-point Storage

Yingchun Zhu,Kunsheng Zhang,Lizhen Ma,Nairui Huo,Hua Yang,Jiaomin Hao 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4

Sensory, physicochemical, and microbiological changes of channel catfish patties stored under controlled freezing-point storage (CFPS) conditions at −1.5℃ for 10 weeks were analyzed to determine useful parameters for freshness evaluation and shelf-life determination. The sensory parameters of aerobic mesophilic and psychrophilic bacterial counts, physicochemical parameters of thiobarbituric acid, total volatile basic nitrogen, chewiness, ATP-ase activity, and the lightness parameter L* were correlated with storage time. Vacuum-packaged patties containing a cryoprotectant, natural antioxidants, and an antimicrobial mixture stored at −1.5℃ exhibited an extended shelf-life from a conventional 1-2 weeks to 6 weeks. Parameters that were correlated with storage time can be used as indicators of quality degradation of patties. CFPS combined with vacuum packaging offers an effective approach to extension of catfish product shelf-life.

Dark Field Digital Holographic Microscopy Based on Two-lens 360-degree Oblique Illumination

Xiuying Zhang,Yingchun Zhao,Caojin Yuan,Shaotong Feng,Lin Wang 한국광학회 2020 Current Optics and Photonics Vol.4 No.3

In this paper we propose a dark-field digital holographic microscopy system based on 360-degree oblique illumination. This setup is constructed without using a dark-field objective. The principle of 360-degree oblique illumination of vortex beam and dark-field digital holographic microscopy are introduced theoretically and experimentally. By analyzing the reconstructed image of a dark-field digital hologram of a USAF 1951 target, it is proved that the imaging resolution can be improved by this method. And also, comparison and analysis are made on the reconstructed image of a bright-dark field digital hologram of a pumpkin stem slice, the result shows that the imaging contrast is also enhanced with this method, and it is effective for dark-field digital holographic microscopy imaging of large transparent biological samples.

The Ontology Design of Intangible Cultural Heritage Based on CIDOC CRM

Jun Hu,Yingchun Lv,Mu Zhang 보안공학연구지원센터(IJUNESST) 2014 International Journal of u- and e- Service, Scienc Vol.7 No.1

A new approach for multi-channel surface EMG signal simulation

Yong Ning,Yingchun Zhang 대한의용생체공학회 2017 Biomedical Engineering Letters (BMEL) Vol.7 No.1

Simulation models are necessary for testing the performance of newly developed approaches before they can be applied to interpreting experimental data, especially when biomedical signals such as surface electromyogram (SEMG) signals are involved. A new and easily implementable surface EMG simulation model was developed in this study to simulate multi-channel SEMG signals. A single fiber action potential (SFAP) is represented by the sum of three Gaussian functions. SFAP waveforms can be modified by adjusting the amplitude and bandwidth of the Gaussian functions. SEMG signals were successfully simulated at different detected locations. Effects of the fiber depth, electrode position and conduction velocity of SFAP on motor unit action potential (MUAP) were illustrated. Results demonstrate that the easily implementable SEMG simulation approach developed in this study can be used to effectively simulate SEMG signals.