Transcriptome analysis of the pheromone glands in Noorda blitealis reveals a novel AOX group of the superfamily Pyraloidea

Zhang Zu-Bing,Yin Ning-Na,Long Ji-Ming,Zhang Yong-Ke,Liu Nai-Yong,Zhu Jiaying 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

Noorda blitealis (Lepidoptera: Pyraloidea: Crambidae) is a major defoliating pest of Moringa trees. Focusing on its mating and reproduction, here we sequenced and analyzed the transcriptome of its pheromone glands (PGs) with a combination of Illumina sequencing, bioinformatics and phylogenetics approaches, coupled with a genomebased analysis. Transcriptome sequencing led to the yields of approximately 162 million clean reads, which were assembled into 60,578 unigenes and 121,692 transcripts, respectively. From the transcriptome, totally 117 genes encoding eight pheromone biosynthesis enzymes and one pheromone degradation enzyme were identified, 90 of which had complete open reading frames. A comparative analysis between PGs and bodies (removing PGs) revealed a large number of differentially expressed genes, including 79 pheromone biosynthesis and degradation related genes. Of the identified genes, NbliDES12 belonging to the △11 desaturase group was likely to a strong candidate for the desaturation of sex pheromones in N. blitealis, as implied by phylogenetic analyses and expression profiles. Finally and most notably, through genome and transcriptome analyses we discovered, for the first time, a novel aldehyde oxidase 6 (AOX6) group of the superfamily Pyraloidea that have been slightly expanded by gene duplications. Moreover, each orthologous AOX group shared highly conserved gene structure. Together, this current study has characterized the genes associated with sex pheromone biosynthesis and degradation from the PG transcriptome of N. blitealis, and more importantly, identifies a novel AOX group of the Pyraloidea.

Tracking the movement trajectory of newly emerged adult Chinese citrus flies with insect harmonic radar

Zhang-Zhang He,Jie Luo,Lian-You Gui,Deng-Ke Hua,Tian-Hua Du,Fu-Lian Wang,Peng Liang,Yong-Fang Shi,Xuan Yang 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3

Previous work reveals that the adult Chinese citrus fly, Bactrocera minax (Enderlein), emerges from the ground in citrus orchards and presumably disperses to alternate food sources in neighboring shrubs and trees, where it stays and becomes reproductively mature, and then later returns to the orchard to lay eggs. We investigated the trajectories of early emerged adult Chinese citrus flies, which were tracked with portable harmonic radar in 2013, 2014, 2015, and 2016 in four different habitats in Hubei province of China that presumably provided food sources for adults. This study we investigated whether early emerged adult Chinese citrus flies migrate into forests. A total of 47 adults were tracked following release in a citrus orchard. Our results showed that a total of 33 adults released in a citrus orchard flew into the forest, and 14 adults that disappeared during the tracking process in the citrus orchard. A total of 10 adults (in 2014) that were tracked and released in the forest flew to the forest. Of the adult flies investigated, 54.5% flew directly into the forest, while the remaining 45.5% finally arrived in the forest after repeatedly adjusting their trajectories. When there were Castanea mollissima Blume (Fagaceae) in the surrounding forest, the proportion of frequency of adult landing on the plant represented 58.3% and 88.5% of landing on all species in 2014 and in 2015, respectively. We conclude that early emerged adult Chinese citrus flies in the mosaic-type citrus orchard of the hilly terrain landform dispersed into adjacent forest.

Clinical Outcomes of Basaloid Squamous Cell Carcinoma of the Esophagus: A Retrospective Analysis of 142 Cases

Zhang, Bai-Hua,Cheng, Gui-Yu,Xue, Qi,Gao, Shu-Geng,Sun, Ke-Lin,Wang, Yong-Gang,Mu, Ju-Wei,He, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.

Adaptive Fault-tolerant Control for Mechanical Manipulator Systems with Actuator Fault

Yong Chen,Bin Guo,Yuezhi Liu,Ke Zhang 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.7

In this paper, the tracking control problem of a class of mechanical manipulator system with actuator fault and mismatched disturbance is investigated. An observer-based adaptive fast finite-time sliding mode fault-tolerant control (OB-AFTSMFTC) scheme is proposed. Firstly, a novel composite observer is designed to estimate the state, fault efficiency factor, and disturbance simultaneously without the disturbance bound information. It is theoretically proved that the entire observer system is globally stable. To address the tracking performance, the Euler’s discretization technique is applied to approximate the discrete-time tracking error model. Subsequently, an OB-AFTSMFTC strategy is proposed based on the tracking error. In the proposed control scheme, two adaptive parameters are designed to realize the autonomous adjustment of controller parameters, thus the work of parameter adjustment can be reduced and a faster convergence can be achieved. Furthermore, the estimated disturbance information is utilized to construct the sliding mode controller in order to improve the robustness of the tracking performance. Finally, two simulation cases are carried out to verify the fault tolerance ability of the proposed method.

Design and testing of CFRP sleeve for a high-speed permanent magnet synchronous motor with surface-mounted rotor

Yong Zhou,Lei Tian,Sheng-Hua Gao,Jing-Wei Zhang,Lin-Ke Yang,Rui-Guang Xie 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.1

A high-speed (HS) permanent magnet (PM) synchronous motor (HSPMSM) with a carbon fiber-reinforced plastic (CFRP) protective sleeve in the surface-mounted rotor was explored in this study. In view of retaining the PMs at HS operations, the high-strength CFRP sleeve was designed on the basis of a process that could be summarized as follows. First, a multi-physics analysis of the rotor was conducted. The requirements to the CFRP sleeve were obtained from electromagnetic analysis, loss analysis, heat flow coupling computational fluid dynamics analysis, structural analysis, and rotor dynamics analysis. Second, the CFRP sleeve was designed by theoretical analysis. From the results, the thickness values of the carbon fibers in the circumferential and helical directions were chosen to be 4 and 1 mm, respectively, while the helical angle of carbon fibers was chosen to be 70°. Then, a plateshaped CFRP product and a cylindrical CFRP product were fabricated to verify the strength and stiffness of the CFRP sleeve. The performance test results show that the hoop strength and elastic modulus and the axial strength and elastic modulus of the plate-shaped CFRP products at 20 °C are 1963 MPa @ 156 GPa and 550 MPa @ 36 GPa, respectively. The hoop strain and axial strain of the cylindrical CFRP product under 35 MPa are about 3000 and 1900 με, which meet the design requirements of the HSPMSM.

Concordance in Cervical HPV Detection between Hybrid Capture 2 and HPV GenoArray Tests

Zhang, Li,Lin, Yong,Li, Jin-ke Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

HPV type-specific detection may promote cervical screening program and vaccination development worldwide. We conduct a study comparing HPV Hybrid capture II (HC II) Test and Hybribio GenoArray test, a newly developed HPV type-specific assay, in patients with cervical epithelial neoplasm. Results showed a good concordance in cervical HPV detection between two tests (kappa value 0.80, p<0.05, McNemar test). Our study may promote utilization of type-specific HPV detection that is helpful for cervical cancer screening and vaccination.

Parallel Control of Shunt Active Power Filters in Capacity Proportion Frequency Allocation Mode

Zhang, Shuquan,Dai, Ke,Xie, Bin,Kang, Yong The Korean Institute of Power Electronics 2010 JOURNAL OF POWER ELECTRONICS Vol.10 No.4

A parallel control strategy in capacity proportion frequency allocation mode for shunt active power filters (APFs) is proposed to overcome some of the difficulties in high power applications. To improve the compensation accuracy and overall system stability, an improved selective harmonic current control based on multiple synchronous rotating reference coordinates is presented in a single APF unit, which approximately implements zero steady-state error compensation. The combined decoupling strategy is proposed and theoretically analyzed to simplify selective harmonic current control. Improved selective harmonic current control forms the basis for multi-APF parallel operation. Therefore, a parallel control strategy is proposed to realize a proper optimization so that the APFs with a larger capacity compensate more harmonic current and the ones with a smaller capacity compensate less harmonic current, which is very practical for accurate harmonic current compensation and stable grid operation in high power applications. This is verified by experimental results. The total harmonic distortion (THD) is reduced from 29% to 2.7% for a typical uncontrolled rectifier load with a resistor and an inductor in a laboratory platform.

Stability Analysis for T-S Fuzzy Systems with Time-varying Delay via Free-matrix-based Integral Inequality

Yong He,Zhi Lian,Chuan-Ke Zhang,Min Wu 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.1

This paper is concerned with the problem of delay-dependent stability criteria for Takagi-Sugeno systemswith time-varying delay. The less conservative delay-dependent stability criteria in terms of linear matrix inequalitiesare obtained by constructing a novel augmented Lyapunov-Krasovskii functional. In order to further improvethe stability conditions, the free- matrix-based integral inequality is employed. Finally, several numerical exampleson time-delay stability analysis are given to demonstrate the effectiveness and the merits of the proposed methods.

Improved Fiber Uniformity and Jet Number in Multi-spinneret Electrospinning via Auxiliary Electrode

Yu-Ke Wu,Zong-Jie Li,Jie Fan,Liang Wang,Peng-Fei Zhang,Jian Liu,Yong Liu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.6

Auxiliary-electrode electrospinning process is a novel technique to improve nanofiber production rate Due to theformation of multiple jets. In the present work, dual-spinneret (DSE) and tri-spinneret electrospinning (TSE) with anauxiliary electrode were employed to evaluate the number of jets and fiber uniformity in this electrospinning process. Theresults showed that all the spinnerets in DSE and TSE with an auxiliary electrode could produce 2-12 jets, which could beadjusted by controlling the process parameters such as applied voltage and injection speed. The number of jets initiallyincreased and then decreased with a rise in applied voltage in these two systems. Moreover, the produced nanofibers weresmaller in size and possessed a more uniform fiber diameter per distribution in comparison to those without the auxiliaryelectrode. This work provides a new insight on mass production of nanofibers and designing electrospinning apparatus.

Effect of the opening of a butterfly valve on the dynamic evolution of cavitation

Guang Zhang,Ze Yong Wu,Ke Xin Wu,Yu Qiong Ou,Heuy Dong Kim,Zhe Lin 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.7

As the key control equipment for the transmission of fluid medium, processing valves are widely used in the transmission systems of fluid medium in energy, chemical industry, metallurgy and other fields, which play important roles in the stability and reliability of the system operation. When the flow cross-section is operated in a sudden change, the pressure decreases rapidly at the downstream, which leads to the cavitation in the processing valves. Cavitation makes serious erosion and damage on the valve core and pipeline surface, which makes the leakage and noise problems in processing valves. This seriously affects the regulation performance and lifetime of processing valves. In this article, numerical simulations were carried out to investigate the transient cavitation in a model butterfly valve. By considering effects of local pressure on formation of cavitation, a modified model for calculating the diameter of cavitation bubbles was derived. Effects of valve opening degree were investigated on the dynamic evolution of cavitation by analyzing formation, development and collapse of cavitation. The generation, development and collapse of single cavitation bubble was obtained and discussed in details to state the interaction between vortices and cavitation. Attached and quasiperiodic cavitation were observed and analyzed at different valve opening degrees in detail as well.