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- 저자 : Yin Ning-Na (검색결과 3 건)
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Antennal UDP-glycosyltransferase genes in the coffee white stemborer, Xylotrechus quadripes
Ning-Na Yin,Yu-Jie Zhao,Jia-Ying Zhu,Nai-Yong Liu 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.4
The antenna of Xylotrechus quadripes is the principle olfactory organ that is subjected to a large number of endogenous and exogenous compounds. The gene families associated with the detoxification of these compounds are essential for the adaptive evolution of insect defensive strategies. However, knowledge on uridine diphosphate (UDP)-glycosyltransferases (UGTs) of X. quadripes is unavailable. Here, we characterized 30 UGT genes identified from an antennal transcriptome of X. quadripes. Among them, 16 UGT genes encoding 508–527 amino acids shared the full-length sequences and signal peptides in N-terminus. Multiple sequence alignment revealed that X. quadripes UGTs had a variable N-terminus and a conserved C-terminus. Phylogenetic analysis showed that X. quadripes UGTs were classified into ten sub-families with the largest UGT one of UGT352 (nine genes) and a strict single copy of UGT50 within coleopteran species. Gene structural analysis indicated that coleopteran UGT50s underwent intron gains or losses. Expression profile revealed that all studied X. quadripes UGTs were transcribed in the antennae of both sexes, some of which exhibited sex-biased expression including UGT2, UGT6, UGT20 and UGT27 in females as well as UGT3, UGT11 and UGT12 in males. In addition, most of UGTs were widely expressed in other tissues, indicating their functional diversities in this beetle. Together, these findings provide valuable information for further functional studies of UGTs in X. quadripes, especially their roles in olfaction.
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Zhao Sihui,Ning Yunfang,Qin Na,Ping Nan,Yu Yong,Yin Guoyan 한국유전학회 2021 Genes & Genomics Vol.43 No.8
Background Asthma is a serious respiratory disease that afects the physical and mental health of children. Airway epithelial apoptosis concomitantly mediated by transforming growth factor-β1 (TGF-β1) is a crucial component of asthma pathogenesis. LncRNA growth Arrest Specifc 5 (GAS5), microRNA-217 (miR-217) and Histone deacetylase 4 (HDAC4) shown a close relationship with TGF-β1-induced injury of airway epithelial. However, the mechanism underlying TGF-β1-induced injury of airway epithelial in asthma still needs to be investigated. Objective We aimed to investigate the efect and underlying mechanism of GAS5/miR-217/HDAC4 axis in TGF-β1- stimulated bronchial epithelial cells. Methods The levels of were detected by quantitative real-time polymerase chain reaction (RT-qPCR). All protein levels were determined by western blot. Cell viability and apoptosis rate were assessed by Methyl thiazolyl tetrazolium (MTT) and Flow cytometry, respectively. The targeting relationship between miR-217 and GAS5 or HDAC4 was examined with dual-luciferase reporter assay. Results TGF-β1, GAS5, HDAC4 were up-regulated, while miR-217 was down-regulated in bronchial mucosal tissues of asthmatic children and TGF-β1-treated BEAS-2B cells. TGF-β1 could reduce cell viability and induce apoptosis, while these efects could be reversed by downregulation of GAS5 or HDAC4. Mechanically, GAS5 acted as a sponge for miR-217 to regulate the expression of HDAC4. Furthermore, overexpression of HDAC4 rescued the efects of GAS5 knockdown on viability and apoptosis of TGF-β1-induced BEAS-2B cells. GAS5 knockdown induced cell viability and hampered cell apoptosis in TGF-β1-stimulated BEAS-2B cells by regulating the miR-217/HDAC4 axis. Conclusions The lncRNA GAS5/miR-217/HDAC4 axis played an important role in regulating TGF-β1-induced bronchial epithelial cells injury, thus contributing to asthma.
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Zhang Zu-Bing,Yin Ning-Na,Long Ji-Ming,Zhang Yong-Ke,Liu Nai-Yong,Zhu Jiaying 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1
Noorda blitealis (Lepidoptera: Pyraloidea: Crambidae) is a major defoliating pest of Moringa trees. Focusing on its mating and reproduction, here we sequenced and analyzed the transcriptome of its pheromone glands (PGs) with a combination of Illumina sequencing, bioinformatics and phylogenetics approaches, coupled with a genomebased analysis. Transcriptome sequencing led to the yields of approximately 162 million clean reads, which were assembled into 60,578 unigenes and 121,692 transcripts, respectively. From the transcriptome, totally 117 genes encoding eight pheromone biosynthesis enzymes and one pheromone degradation enzyme were identified, 90 of which had complete open reading frames. A comparative analysis between PGs and bodies (removing PGs) revealed a large number of differentially expressed genes, including 79 pheromone biosynthesis and degradation related genes. Of the identified genes, NbliDES12 belonging to the △11 desaturase group was likely to a strong candidate for the desaturation of sex pheromones in N. blitealis, as implied by phylogenetic analyses and expression profiles. Finally and most notably, through genome and transcriptome analyses we discovered, for the first time, a novel aldehyde oxidase 6 (AOX6) group of the superfamily Pyraloidea that have been slightly expanded by gene duplications. Moreover, each orthologous AOX group shared highly conserved gene structure. Together, this current study has characterized the genes associated with sex pheromone biosynthesis and degradation from the PG transcriptome of N. blitealis, and more importantly, identifies a novel AOX group of the Pyraloidea.
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