위절제술 후 발생한 유미성 복수 -증례 보고-

윤효영, 송영진, 류동희, 김대훈 충북대학교 의과대학 충북대학교 의학연구소 2011 忠北醫大學術誌 Vol.21 No.2

-본문참고-

담도-위 누공 : 증례보고

윤효영,류동희,송영진 충북대학교 의과대학 충북대학교 의학연구소 2005 忠北醫大學術誌 Vol.15 No.2

위궤양에 의한 담관-위 누공은 매우 드문 질환이며 소화성 궤양에 의한 담관 누공의 경우 대부분 내과적 치료가 우선적으로 시행된다. 저자들은 내과적 치료에 호전되지 않는 궤양성 담관-위 누공을 외과적 절제술을 시행하여 완치하였기에 문헌고찰과 함께 보고하는 바이다. 64세 남자 환자가 15년 동안 지속된 식사 후 복통과 우상복부 동통을 주소로 내원하였다. 위 내시경 소견상 위전정부, 소만곡 후벽에 담즙이 역류하는 궤양저가 관찰되었으며 상복부위장관 촬영상 위전정부와 총수담관이 교통하는 소견이 있었으나 전산화복부단층 소견상 담석의 증거는 없었다. 담관-위누공 의심하에 체간미주신경절단술, 유문절제술, 담낭절제술, 총수담관절제술 후 환자는 퇴원하였다. Choledochogastric fistula complicated from gastric ulcer is very rare. Most of the internal biliary fistula by peptic ulcer were successfully managed with conservative treatment. Authors reported successful surgical resection of choledochogastric fistula caused by gastric ulcer. A (54-year old man visited our hospital complaining of postprandial abdominal pain and right upper quadrant pain for 15 years. He has a history of peptic ulcer medication for four years, recently. A gastrofiberscopy revealed ulceration on the posterior wall along the lesser curvature, gastric antrum with bile reflux from ulcer base, and histopathology of the biopsy specimen revealed an ulcer without evidence of malignancy. Barium upper gastrointestinal study showed fistula between stomach and common bile duct. In abdominal CT, there was a pneumobilia with dilated intrahepatic duct without common bile duct dilatation. After the diagnosis of choledochogastric fistula, we performed truncal vagotomy with antrectomy and cholecystectomy with resection of extrahepatic bile duct. The patient has remained well since his discharge from hospital.

다발성 장 천공을 동반한 베체트병 - 1예 보고 -

윤효영,이상전,송영진 대한대장항문학회 1995 Annals of Coloproctolgy Vol.11 No.2

TGA 방법을 이용한 플라스틱과 하수·분뇨슬러지의 열분해 반응속도 연구

윤효숙,최성필,김성탁,원양수,신부영 嶺南大學校 環境問題硏究所 1997 環境硏究 Vol.16 No.2

요약문 도시 고형폐기물의 열분해 특성에 대한 기초 자료를 제시하고자 열중량분석기를 이용하여 가열속도를 실험변수로 하여 하수 및 분뇨슬러지와 5대 범용 플라스틱인 LDPE, HDPE, PP, PS 와 PVC의 열분해 반응속도인자 및 분해특성을 파악하였다. 활성화에너지는 가열속도에 상관없이 일정한 값을 나타내었으며 분해속도가 최고일때의 온도는 가열속도가 증가함에 따라 증가하였다. LDPE, HDPE, PP와 PS는 1단계 분해반응으로 350∼520 ℃에서 대부분이 완전분해되었으며, PVC는 2단계 분해반응으로 250∼550 ℃에서 분해가 진행되었다. 슬러지는 여러 단계의 분해반응으로 특정한 분해구간이 없이 전구간에 걸쳐서 분해가 진행되었다. PVC를 제외한 플라스틱은 완전분해되었으며, PVC는 약 10%의 잔류량이 있었고, 슬러지의 잔류량은 D 하수, S 하수, T 분뇨 슬러지의 순으로 43, 70, 28.5%를 나타내었다.Abstract The thermal decomposition of sewage/night soil sludge and LDPE, HDPE, PP, PS and PVC was studied by using a conventional dynamic thermogravimetric analyzer (TGA) in a flowing nitrogen environment at several heating rates between 5 ℃ and 40 ℃/min. The dynamic thermogravimetric analysis curve was analyzed to obtain the information on the kinetic parameters such as activation energy, reaction order, and Arrhenius preexponential factor. The activation energy keeps constant regardless of heating rates, and the temperature of he fastest decomposition increases with the heating rate. LDPE, HDPE, PP and PS were completely decomposed in the temperature range of 350∼520 ℃ including as the first step decomposition and PVC was decomposed in the temperature range of 250∼550 ℃ including the second step decomposition. Sludge was decomposed without specific temperature range as several decomposition step. Except of PVC, all plastics were decomposed completely. PVC keeps the remains of about 10%, and sludge keeps the remains of 43% for D sewage sludge, 70% for S sewage sludge, 28.5% for T night soil sludge.

바이오인포메틱스에 기반을 둔 웹 페이지 분석 도구

윤효근,이상용 공주대학교 생산기술연구소 2001 論文集 Vol.9 No.-

This paper proposes a new webpage analysis tool based on bioinformatics. The tool puts web pages into single chromosome and expresses each character and image in the pages by gene, DNA. It analyzes similarity of gene by PCR during the process. Similarity of web page is calculated using fitness Function of gene algorithm.

1~2기 본태성 고혈압 환자들에서 Imidapril 대 Enalapril의 항고혈압 효과를 평가하기 위한 무작위, 이중맹검 임상시험

최영진,손대원,김용진,이홍자,채인호,김효수,김철호,오병희,이명묵,박영배,채윤식,이영우 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11

거대 위점막하종양(위장관 간질종양)의 치료 : 증례보고

윤효영,송영진 충북대학교 의과대학 충북대학교 의학연구소 2009 忠北醫大學術誌 Vol.19 No.2

Non-disruptive and complete en bloc resection must be used in all cases of GISTs (gastrointestinal stromal tumors). Recurrence of GISTs is closely related with mitotic count and tumor size generally, besides, GISTs larger than 10 cm have high recurrence rate. A 77-year-old female presenting with abdominal mass was referred to our institute. Computed tomography (CT) image revealed a huge tumor with central necrosis (21x17x12 cm in size) posterior wall of the stomach, the pancreas and the transverse colon. There was no nodal or distant metastasis on the CT scan. Diagnosis of Gastrointestinal stromal tumor (GIST) was confirmed by endoscopic ultrasonogram (EUS) and we successfully excised the tumor by partial resection of the stomach. Pathological examination and immunohistochemical staining of the specimen showed that mitotic count was more than 10/50HPF, surgical margin was negative, and CD117, CD34 were positive. After 7 months of surgery, newly appeared abdominal mass (8 cm) was detected in right lower quadrant. Therefore, chemotherapy using imatinib mesylate (Gleevec) at a dose of 400 mg daily was preceded and the tumor disappeared. After 19 months of surgery, newly appeared abdominal masses were detected around pancreas, splenic hilum and right lower quadrant of abdomen on the CT scan. 위장관간질종양의 절제는 종양 피막을 손상시키지 않고 완전 절제하는 것이 중요하다. 위장관간질종양의 재발은 유사분열 수, 종양의 크기와 밀접한 관계가 있으며 종양의 크기가 10 cm 이상의 거대 종양의 경우 재발 가능성이 매우 높다. 77세 여자 환자가 복부 종괴를 주소로 내원하였다. 복부 전산화단층 촬영상 종양의 크기는 21×17×12 cm 이었다. 위장관간질종양 의심 하에 위부분절제술 및 종양절제술을 시행하였다. 조직검사상 종양의 무게는 730 gm, 유사분열 수는 50 HPF에서 10개 이상이었으며, CD117 및 CD34는 양성이었다. 환자는 수술 후 7개월째 우하복부에 장경 8cm의 종양이 관찰되어 imatinib mesylate 400 mg 투여 후 소실되었으나 이후 환자는 외래에서 주기적으로 추적 관찰 중 수술 후 19개월째 췌장 주변 및 비문, 우하복부 등에 다발성 종양이 새로 발견되었다.

Roxythromycin-tylosin-furaltadone 합제의 닭 유래 세균에 대한 시험관내 항균효과

김지영,채미경,윤효인,서상희,박배근,김현수,전무형 충남대학교 수의과대학 동물의과학연구소 2003 動物醫科學硏究誌 Vol.11 No.-

In vitro antimicrobial susceptibility of roxythromycin (Rx), tylosin (Tyl), erythromycin (EM) and furaltadone (Fur) was studied, either by the single type or the combinated type of Rx-Tyl-Fur (RTF) against E. co1i (30 strains), Staph. aureus (20 strains) and Mycoplasma gallisepticum (5 strains) that are the major pathogens in poultry farm. The paper disk diffusion and MIC tests were applied. In screening test by paper disk diffusion method, the single types of antibiotics showed the sensitivity against 55 strains of bacteria in order of Fur, EM, Rx and Tyl. The combinated RTF showed the higher rate of sensitivity (80∼83.3% at 64㎍/disk, 60-75% at 32㎍/disk) and wider range of growth inhibiting zone (19.6±3.7-24.8±4.7mm) than those of the single drugs. In MIC test, the sensitivity against the 55 strains appeared in order of Fur. EM, Rx and Tyl as similar to those of paper disk diffusion method. The MIC ranges of RTF were 0.125∼16㎍/ml for E. coli, 0.25∼16㎍/ml for Staph. aureus and 1-8㎍/ml for Myco. gallisepticum, showing evidently lower than those of the single types. The MIC_(50) and MIC_(90) of RTF were estimated l∼4㎍/ml and 8∼16㎍/ml, respectively, and remarkably lower than those of the single drugs.

Self-etching primer를 이용하여 접착된 교정용 브라켓의 전단결합강도

안윤표,김효영,전영미,김정기 대한치과교정학회 2003 대한치과교정학회지 Vol.33 No.1

본 연구는 6세대 교정용 접착 시스템인 Transbond Plus Self-Etching Primer(3M/Unitek Dental Products, Monrovia, Calif)를 이용하여 법랑질면에 브라켓을 접착하는 방법과, 통상적인 산부식 방법에 의해 브라켓을 접착한 경우의 전단결합강도 차이를 비교·평가하고, self etching primer를 이용하여 브라켓을 접착할 때 치면에 존재하는 수분이 브라켓의 전단결합강도에 미치는 영향에 관하여 조사하였다. 37% 인산용액과 Self-Etching Primer를 잉용하여 법랑질을 표면 처리하고 Transbond XT를 이용하여 브라켓을 치면에 부착하였다. 또한 수분의 존재에 따른 전단결합강도의 차이를 평가하기 위해 인공타액을 치면에 도포후 Self-Etching Primer를 사용하여 브라켓을 부착한 후 30분과 24시간에 따른 전단결합강도를 비교 평가하여 다음과 같은 결론을 얻었다. 1. Self-etching primer군에서 건조군과 습윤군의 전단결합강도는 37%인산처리군의 결합강도보다 낮았다(p<0.05). 그러나 Self-etching primer군의 전단결합강도는 임상적으로 유용한 수준의 이상이었다. 2. 모든군에서 24시간군의 전단결합강도가 30분군의 전단결합강도보다 높았으며(p<0.05), 이는 브라켓 접착후 일정시간의 경과가 결합강도를 증가시켜 줌을 알 수 있었다. 3. Self-etching primer군에서 습윤군의 전단결합강도는 건조군보다 높은 경향이었으나 통계적 유의성은 인정되지 않았다(p<0.05). 4. ARI 점수의 비교결과 인산처리군에서는 0점과 1점의 빈도가 높았으며, Self-etching primer 건조군과 습윤군에서는 2점과 3점의 빈도가 높아(p<0.05) Self-etching primer군이 인산처리군보다 법랑질-레진 접착계면부위에서의 파절이 많이 일어남을 알 수 있었다. The purpose of this study was to evcaluate the effects of a self-etching primer on the shear bond strength of orthodontic brackets and on the failure pattern of bracket-adhesive interfaces in dry or wet condition. Brackets were bonded to extracted human teeth according to one of three experimental protocols. In the Group P, teeth were etched with 37% phosphoric acid. After the Transbond XT Primer was applied onto the etched surfaces, the brackets were bonded with Transbond XT(3M, Unitek, Monrovia, Calif) and light cured for 40 seconds. In the Group SD, a self-etching primer(3M, Unitek, Monrovia, Calif) was placed on the enamel for 3 seconds and gently evaporated with air, as suggested by the manufacturer. The brackets were then bonded with Transbond XT as in the Group P. In the Group SW, artificial saliva was applied to the enamel surface for 10 seconds to allow complete hydration of the surface before application self-etching primer. The brackets were then bonded following the procedures of Group SD. Each group was divided into 2 sub-groups(0.5h, 24h) according to debonding time shear bond strengths were measured by Instron universal testing machine. After debonding, the teeth and brackets were examined under scanning electron microscope and assessed with the adhesive remnant index. The result obtained were summarized as follows ; 1. The shear bond strengths were high enough to use clinically in all testing groups, but the shear bond strengths of group SD and SW were significantly lower than Group P(p<0.05). 2. With respect to comparison of debonding time, 24h debonding samples exhibited heigher shear bond strength than 0.5h debonding samples in Group P, SD and SW(p<0.05). 3. In the self etching primer groups(Group SD and Group SW), there was no significant difference in mean shear bond stredifference in mean shear bond strength between under dry and wet state(p>0.05). 4. There was a greater frequency of ARI score of 0 and 1 with the Group P. On the other hand, there was a higher frequency of ARI scores of 2 and 3 with Group SD and Group SW(p<0.05).,

제조합균주 E. coli Ck1092가 생산하는 2,3-Dihydroxybiphenyl Dioxygenase의 정제 및 특성

박효남,김영수,김영창,김치경,임재윤 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.3

4-CB 분해균주인 Pseudomonas sp. P20으로부터 pcbC 유전자를 클로닝하여 얻은 E. coli CK1092로부터 2,3-DHBP dioxygenase을 분리, 정제하여 효소적 특성을 조사하였다. 효소의 정제는 acetone 침전, DEAE-Se-phadex A-25 ion exchange chromatography, preparative electrophoresis 방법으로 정제하였다. 2,3-DHBP dioxygenase의 분자량은 약 270 kDa으로 추정되며, SDS-PAGE에 의한 분자량은 34 kDa이였다. 따라서, 동일한 subunit 8개 존재하는 octamer로 추정된다. 이 효소는 2,3-DHBP에 대해 높은 기질특이성을 보였으며, 3-methylcatechol, 4-methylcatechol, 4-chlorocatechol에 대해서는 활성을 보이지 않았다. 2,3-DHBP에 대한 Km 값은 18 μM이였으며 30μM 이상의 기질농도에서 활성이 감소하였다. 효소활성의 최적 pH는 8.0이였으며, pH 7.0~10.0 범위에서는 안정하였고, 최적 활성 온도는 40~60℃이며, 60℃까지는 비교적 안정하였다. 또한, 이 효소는 Cu^2+, Fe^2+, Fe^3+ 이온들에 의하여 효소활성이 저해되었고, H_2O_2와 EDTA에 의해서도 활성이 저해되었으며, 10%의 유기용매에 의해서 안정화되지 않았다. 효소활성부위를 알아보기 위해 화학변형제를 처리해 본 결과 tryosine, tryptophan과 histidine이 효소활성에 관여할 것으로 추정된다. 2,3-DHBP dioxygenase was purified from E. coli CK1092 carrying the pcbC gene, which was cloned from 4-chlorobiphenyl-degrading Pseudomonas sp. P20. Purification of this enzyme was done by acetone precipitation, DEAE-Sephadex A-25 ion exchange chromatography, and preparative gel electrophoresis. The molecular weight of subunit was 34 kDa determined by SDS-PAGE, and that of native enzyme was about 270 kDa. It suggests that this enzyme consist of eight identical subunits. This enzyme was specifically active against only 2,3-DHBP as a substrate with 18 μM of Km value, but not catechol, 3-methylcatechol, 4-methylcatechol and 4-chlorocatechol. The optimal pH and temperature of 2,3-DHBP dioxygenase were pH 8.0 and 40~60℃. The enzyme was inhibited by Cu^2+, Fe^2+ and Fe^3+ ions, and was inactivated by H_2O_2 and EDTA. The lower concentrations of some organic solvents such as acetone and ethanol don't stabilize the activity of 2,3-DHBP dioxygenase. The enzyme was completely inactivated by adding the reagents such as N-bromosuccinimide, iodine and p-diazobenzene sulfonic acid.