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조팝나무 揷木時期 및 揷床土의 種類가 發根에 미치는 影響
趙鎭泰,尹汰,金泰重,洪承敏,白基燁 한국화훼연구회 1995 화훼연구 Vol.4 No.1
Cutting experiment was conducted to develop a mass propagation method for Spiraea prunifolia var. simpliciflora Nakai. The optimum season for hardwood cutting was between December and early April, but cutting could be done year-round by using rhizome. NAA treatment (100 ppm) was the most effective as rooting promoter. IBA and IAA were also found to be effective. The effect of sand, red clay, loam, silty loam and vermiculite had little differences in rooting especially when cuttings were done earlier than April 5.
Jo, Yun Kee,Seo, Jeong Hyun,Choi, Bong-Hyuk,Kim, Bum Jin,Shin, Hwa Hui,Hwang, Byeong Hee,Cha, Hyung Joon American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.22
<P>During implant surgeries, antibacterial agents are needed to prevent bacterial infections, which can cause the formation of biofilms between implanted materials and tissue. Mussel adhesive proteins (MAPs) derived from marine mussels are bioadhesives that show strong adhesion and coating ability on various surfaces even in wet environment. Here, we proposed a novel surface-independent antibacterial coating strategy based on the fusion of MAP to a silver-binding peptide, which can synthesize silver nanoparticles having broad antibacterial activity. This sticky recombinant fusion protein enabled the efficient coating on target surface and the easy generation of silver nanoparticles on the coated-surface under mild condition. The biosynthesized silver nanoparticles showed excellent antibacterial efficacy against both Gram-positive and Gram-negative bacteria and also revealed good cytocompatibility with mammalian cells. In this coating strategy, MAP-silver binding peptide fusion proteins provide hybrid environment incorporating inorganic silver nanoparticle and simultaneously mediate the interaction of silver nanoparticle with surroundings. Moreover, the silver nanoparticles were fully synthesized on various surfaces including metal, plastic, and glass by a simple, surface-independent coating manner, and they were also successfully synthesized on a nanofiber surface fabricated by electrospinning of the fusion protein. Thus, this facile surface-independent silver nanoparticle-generating antibacterial coating has great potential to be used for the prevention of bacterial infection in diverse biomedical fields.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2014/aamick.2014.6.issue-22/am505784k/production/images/medium/am-2014-05784k_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am505784k'>ACS Electronic Supporting Info</A></P>
Jo, Yun Kee,Choi, Bong-Hyuk,Zhou, Cong,Ahn, Jin-Soo,Jun, Sang Ho,Cha, Hyung Joon The Royal Society of Chemistry 2015 Journal of Materials Chemistry B Vol.3 No.41
<P>Successful titanium implantation strongly depends on early fixation through an osseointegration between the titanium fixture and adjacent bone tissue. From a clinical perspective, rapid recruitment of functional biomolecules from the blood and osteogenic cell binding is critical for osseointegration immediately after implant insertion. Thus, surface modifications aiming to improve the interactions between the blood and implant and to enhance the binding of osteogenic cells onto the implant surface can contribute to successful osseointegration. Mussel adhesive proteins (MAPs) derived from marine mussels have been considered as promising bioadhesives that have strong adhesion and coating abilities onto organic and inorganic surfaces, even in wet environments. Here, we investigated the<I>in vitro</I>and<I>in vivo</I>osteostimulating ability of the bioengineered mussel glue MAP-RGD, which is a recombinant MAP fused with an Arg-Gly-Asp (RGD) peptide, an effective cell recognition motif for activating intracellular signaling pathways, using a titanium mesh (Ti-mesh) as a model titanium implant. We found that the<I>in vitro</I>cell behaviors of pre-osteoblast cells, such as attachment, proliferation, spreading, and osteogenic differentiation, increased significantly on the MAP-RGD-coated Ti-mesh surface.<I>In vitro</I>blood responses including blood wetting, blood clotting, and platelet adhesion were also highly enhanced on the MAP-RGD-coated surface. Importantly, implantation of the MAP-RGD-coated Ti-mesh resulted in a remarkable acceleration of<I>in vivo</I>bone regeneration and maturation of a new bone in a rat calvarial defect. Consequently, the bioengineered mussel glue can be successfully utilized as an osteostimulating bone bioadhesive for titanium implant applications with further expansion to general bone tissue engineering.</P>