Combination Doxorubicin and Interferon-α Therapy Stimulates Immunogenicity of Murine Pancreatic Cancer Panc02 Cells via Up-regulation of NKG2D ligands and MHC Class I

Wang, Wen-Jia,Qin, Si-Hao,Zhang, Ji-Wei,Jiang, Yue-Yao,Zhang, Jin-Nan,Zhao, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Pancreatic adenocarcinoma is a malignant gastrointestinal cancer with significant morbidity and mortality. Despite severe side effects of chemotherapy, the use of immunotherapy combined with chemotherapy has emerged as a common clinical treatment. In this study, we investigated the efficacy of the combined doxorubicin and interferon-${\alpha}$ (IFN-${\alpha}$) therapy on murine pancreatic cancer Panc02 cells in vitro and in vivo and underlying mechanisms. Materials and Methods: A Panc02-bearing mouse model was established to determine whether doxorubicin and interferon-${\alpha}$ (IFN-${\alpha}$) could effectively inhibit tumor growth in vivo. Cytotoxicity of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) was evaluated using a standard LDH release assay. To evaluate the relevance of NK cells and CD8 T cells to the combination therapy-mediated anti-tumor effects, they were depleted in tumor-bearing mice by injecting anti-asialo-GM-1 antibodies or anti-CD8 antibodies, respectively. Finally, the influence of doxorubicin+interferon-${\alpha}$ (IFN-${\alpha}$) on the ligands of NK and T cells was assessed by flow cytometry. Results: The combination therapy group demonstrated a significant inhibition of growth of Panc02 in vivo, resulting from activated cytotoxicity of NK cells and CTLs. Depleting CD8 T cells or NK cells reduced the anticancer effects mediated by immunochemotherapy. Furthermore, the doxorubicin+IFN-a treatment increased the expression of major histocompatibility complex class I (MHC I) and NKG2D ligands on Panc02 cells, suggesting that the combined therapy may be a potential strategy for enhancing immunogenicity of tumors. All these data indicate that the combination therapy using doxorubicin and interferon-${\alpha}$ (IFN-${\alpha}$) may be a potential strategy for treating pancreatic adenocarcinoma.

Nanoscale Printing Technique and its Applications in Nanophotonics

Huacun Wang,Long Wen,Xin Hu,Yan Yu,Yue Zhao,Qin Chen 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.9

"Printing" is an ancient technique for recording and publishing. Recently, this technique has extended its activities down to a nanometer scale. In this paper, we review the progress of the printing technique focusing on the most recent developments of one technique called "transfer printing" and its applications in nanophotonics.

Conceptual design for a multi-rotor UAV based on variable paddle pitch

Zhen Qin,Xiaoran Tang,Ziqi Meng,Yu-Ting Wu,류성기,Yue Wang 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.10

The field of unmanned aerial vehicles has experienced a "golden era" of progress in recent years (UAV). UAVs come in a vast variety, from the commercial to the military sectors. The UAVs available today are tiny, mini multirotor devices. The advantages of the small multi-rotor structure are its small size, straightforward design, high hovering effectiveness, adaptability in changing directions, and quick flight speed. For activities like tracking and aerial photography, it is especially useful. However, the majority of multi-rotor UAVs, according to current UAV offers, are built around cameras and sensors. As a result, they can't complete other kinds of missions due to their large overall size, light weight, and small cargoes. The outbreak has sparked interest in and study into whether contactless distribution and logistics are practical. Unmanned mechanical devices for various rescue and specialized duties are also being researched by organizations. Therefore, a medium to big multi-rotor UAV needs to be researched and developed. The assembly of paddle holders and auto-tilters for medium- to large-sized multi-rotor UAVs is examined in this work. The multi-rotor UAV is intended to mount an auto-tilter. Its component strength checks are analyzed. Additionally, the blade requirements are chosen, along with strength tests of the individual components, and the structural design of the UAV rotor system is provided. The mechanical viability of the UAV is then confirmed using a motion simulation of its mechanical design.

Bioassay-guided Isolation of a Novel Protein with Antitumor Activity from Trachyrhamphus serratus (Syngnathidae)

Meng Yue Wang,Sun Xing Qin,Fen He,Xiaobo Li 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.6

A novel protein with antitumor activity, Hailongin, was purified from the aqueous extract of the whole body of Trachyrhamphus serratus, which is commonly used in traditional Chinese medicine, by bioassay-guided fractionation. Hailongin exhibited strong inhibition of proliferation of the tested human cell lines, such as A549, HeLa, LoVo and CCRF-CEM. The IC_50 values of Hailongin ranged from 5.4 to 25.7 μg/mL. An in vivo study showed that the growth of implanted S-180 solid tumors in mice was significantly inhibited by Hailongin treatment, while the immunological function of the tumor-bearing mice was enhanced. The molecular weight and the isoelectric point of Hailongin were 57.074 kDa (by MALDI-TOF-MS) and 6.2 (by isoelectric focusing-polyacrylamide gel electrophoresis), respectively. Seventeen amino acids were identified in Hailongin. The acidic amino acids accounted for the majority of Hailongin’s amino acid composition. The N-terminal amino acid sequence of Hailongin was determined to be IVPYSHNAGNKGLTQMR and showed no significant homology with known proteins.

An electrode universal and self-healable integrated supercapacitor fabricated by physical adsorption based on mussel-inspired highly adhesive gel electrolyte

Gang Qin,Yue Wang,Wenjie He,Da Zhang,Xiaojuan Yu,Fenghan Gong,Hengyi Yu,Xiang Yu,Jia Yang,Qiang Chen 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.122 No.-

Integrated supercapacitor with stable electrochemical performance under complex deformations is theprospective energy storage device for wearable electronics. However, there is often a conflict betweenthe mechanical performance and the specific capacitance of the conventional conductive polymerbasedintegrated supercapacitor. In this work, a novel integrated supercapacitor based on adhesive gelelectrolyte has been prepared by physical adsorption, which not only avoids the drawbacks of the conventionalintegrated supercapacitor but also exhibits the electrode universality including powder electrodematerial. The adhesive triple network polyacrylamide/polydopamine/agar/H3PO4 gel electrolyteis fabricated with high ionic conductivity, toughness and adhesion. Based on this, the integrated supercapacitoris assembled by spraying activated carbon powder on both sides of the pre-stretched biaxiallygel electrolyte. Due to the strong interfacial adhesion, such a device exhibits low interfacial resistance andhigh specific capacitance of 155 mF/cm2 at 0.5 mA/cm2. More impressively, the capacitance retention canreach 92.4% after 10 cutting/healing cycles and even keep the brilliant self-healabillity at 20 C. Additionally, it can hold stable electrochemical performance when exposed to harsh environments. The physical adsorption strategy would offer novel inspiration for the development of integrated supercapacitorand has great practical applications in wearable and portable electronics.

Absence of EZH2 Gene Mutation in Chronic Myeloid Leukemia Patients in Blast Crisis

Chen, Hao-Yue,Yao, Hong,Wu, Ling-Yu,Liu, Can-Jun,Zhu, Jian-Qin,Liu, Chun-Hua,Wang, Wei,Dong, Sha-Sha,Ping, Na-Na,Chen, Su-Ning,Sun, Miao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Reports ; Adrenergic receptor β2 activation by stress promotes breast cancer progression through macrophages M2 polarization in tumor microenvironment

( Jun-fang Qin ),( Feng-jiao Jin ),( Ning Li ),( Hai-tao Guan ),( Lan Lan ),( Hong Ni ),( Yue Wang ) 생화학분자생물학회 2015 BMB Reports Vol.48 No.5

Stress and its related hormones epinephrine (E) and norepinephrine (NE) play a crucial role in tumor progression. Macrophages in the tumor microenvironment (TME) polarized to M2 is also a vital pathway for tumor deterioration. Here, we explore the underlying role of macrophages in the effect of stress and E promoting breast cancer growth. It was found that the weight and volume of tumor in tumor bearing mice were increased, and dramatically accompanied with the rising E level after chronic stress using social isolation. What is most noteworthy, the number of M2 macrophages inside tumor was up-regulated with it. The effects of E treatment appear to be directly related to the change of M2 phenotype is reproduced in vitro. Moreover, E receptor ADRβ2 involved in E promoting M2 polarization was comprehended simultaneously. Our results imply psychological stress is influential on specific immune system, more essential for the comprehensive treatment against tumors. [BMB Reports 2015; 48(5): 295-300]

The Regulation of LexA on UV-Induced SOS Response in Myxococcus xanthus Based on Transcriptome Analysis

( Duo-hong Sheng ),( Ye Wang ),( Shu-ge Wu ),( Rui-qin Duan ),( Yue-zhong Li ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.7

SOS response is a conserved response to DNA damage in prokaryotes and is negatively regulated by LexA protein, which recognizes specifically an “SOS-box” motif present in the promoter region of SOS genes. Myxococcus xanthus DK1622 possesses a lexA gene, and while the deletion of lexA had no significant effect on either bacterial morphology, UV-C resistance, or sporulation, it did delay growth. UV-C radiation resulted in 651 upregulated genes in M. xanthus, including the typical SOS genes lexA, recA, uvrA, recN and so on, mostly enriched in the pathways of DNA replication and repair, secondary metabolism, and signal transduction. The UV-irradiated lexA mutant also showed the induced expression of SOS genes and these SOS genes enriched into a similar pathway profile to that of wild-type strain. Without irradiation treatment, the absence of LexA enhanced the expression of 122 genes that were not enriched in any pathway. Further analysis of the promoter sequence revealed that in the 122 genes, only the promoters of recA2, lexA and an operon composed of three genes (pafB, pafC and cyaA) had SOS box sequence to which the LexA protein is bound directly. These results update our current understanding of SOS response in M. xanthus and show that UV induces more genes involved in secondary metabolism and signal transduction in addition to DNA replication and repair; and while the canonical LexA-dependent regulation on SOS response has shrunk, only 5 SOS genes are directly repressed by LexA.

Domain Adaptation Image Classification Based on Multi-sparse Representation

( Xu Zhang ),( Xiaofeng Wang ),( Yue Du ),( Xiaoyan Qin ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.5

Generally, research of classical image classification algorithms assume that training data and testing data are derived from the same domain with the same distribution. Unfortunately, in practical applications, this assumption is rarely met. Aiming at the problem, a domain adaption image classification approach based on multi-sparse representation is proposed in this paper. The existences of intermediate domains are hypothesized between the source and target domains. And each intermediate subspace is modeled through online dictionary learning with target data updating. On the one hand, the reconstruction error of the target data is guaranteed, on the other, the transition from the source domain to the target domain is as smooth as possible. An augmented feature representation produced by invariant sparse codes across the source, intermediate and target domain dictionaries is employed for across domain recognition. Experimental results verify the effectiveness of the proposed algorithm.

아드만탄 기반의 새로운 설포네이트 폴리카보네이트 난연제 성능 연구

Jian Wei Guo,Yue Qin Wang,Li Juan Feng,Xing Zhong,Chu Fen Yang,Sa Liu,Ying De Cui 한국고분자학회 2013 폴리머 Vol.37 No.4

A novel sulfonate flame retardant, 1,3,5,7-tetrakis(phenyl-4-sodium sulfonate)adamantane (FR-A), was suc-cessfully synthesized from l-bromoadamantane in sequential four-step reactions involving Fiedel-Crafts phenylation, sul-phonation, hydrolysis, and neutralization. The success of synthesis was confirmed by FTIR spectra, ‘H NMR spectra, elemental analyses and mass spectra. The effect of FR-A on the flame retardacy of polycarbonate (PC) has been studied. Limiting oxygen index (LOI) and thermogravimetric analysis (TGA) showed that this novel sulfonate flame retardant had effective flame retardancy on polycarbonate (PC). With a small amount (0.08 Wt%) of FR-A. the flame retardancy of PC was improved obviously, which got to UL 94 V-0 rating. TGA and DTA curves demonstrated that the additive raised the degradation rate of PC by promoting the quick formation of an insulating carbon layer on the surface, and confirmed that the flame retardant mechanism of PC/FR-A system was similar to potassium diphenylsulfone sulfonate (KSS).