Combination of Tumor Volume and Epstein-Barr Virus DNA Improved Prognostic Stratification of Stage II Nasopharyngeal Carcinoma in the Intensity Modulated Radiotherapy Era: A Large-Scale Cohort Study

Qiu-Yan Chen,Shao-Yan Guo,Lin-Quan Tang,Tong-Yu Lu,Bo-Lin Chen,Qi-Yu Zhong,Meng-Sha Zou,Qing-Nan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Yang Li,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Chong Zha 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose Little is known about combination of the circulating Epstein-Barr viral (EBV) DNA and tumor volume in prognosis of stage II nasopharyngeal carcinoma (NPC) patients in the intensity modulated radiotherapy (IMRT) era. We conducted this cohort study to evaluate the prognostic values of combining these two factors. Materials and Methods By Kaplan-Meier, we compare the differences of survival curves between 385 patients with different EBV DNA or tumor volume levels, or with the combination of two biomarkers mentioned above. Results Gross tumor volume of cervical lymph nodes (GTVnd, p < 0.001) and total tumor volume (GTVtotal, p < 0.001) were both closely related to pretreatment EBV DNA, while gross tumor volume of nasopharynx (GTVnx, p=0.047) was weakly related to EBV DNA. EBV DNA was significantly correlated with progress-free survival (PFS, p=0.005), locoregional-free survival (LRFS, p=0.039), and distant metastasis-free survival (DMFS, p=0.017), while GTVtotal, regardless of GTVnx and GTVnd, had a significant correlation with PFS and LRFS. The p-values of GTVtotal for PFS and LRFS were 0.008 and 0.001, respectively. According to GTVtotal and pretreatment EBV DNA level, patients were divided into a low-risk group (EBV DNA 0 copy/mL, GTVtotal < 30 cm3; EBV DNA 0 copy/mL, GTVtotal  30 cm3; or EBV DNA > 0 copy/mL, GTVtotal < 30 cm3) and a high-risk group (EBV DNA > 0 copy/mL, GTVtotal  30 cm3). When patients in the low-risk group were compared with those in the high-risk group, 3-year PFS (p=0.003), LRFS (p=0.010), and DMFS (p=0.031) rates were statistically significant. Conclusion Pretreatment plasma EBV DNA and tumor volume were both closely correlated with prognosis of stage II NPC patients in the IMRT era. Combination of EBV DNA and tumor volume can refine prognosis and indicate for clinical therapy.

Pretreatment Serum Amyloid A and C-reactive Protein Comparing with Epstein-Barr Virus DNA as Prognostic Indicators in Patients with Nasopharyngeal Carcinoma: A Prospective Study

Qiu-Yan Chen,Qing-Nan Tang,Lin-Quan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Chao-Feng Li,Yang Li,Yu-Jing Liang,Xue-Song Sun,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Yu-Ying Fan,Yan He,Ming-Yuan C 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose The measuring Epstein-Barr virus (EBV) DNA is an important predictor of nasopharyngeal carcinoma (NPC). This study evaluated the predictive value of pretreatment serum amyloid A (SAA) and C-reactive protein (CRP) comparing with EBV DNA in patients with NPC. Materials and Methods In an observational study of 419 non-metastatic NPC patients, we prospectively evaluated the prognostic effects of pretreatment SAA, CRP, and EBV DNA on survival. The primary endpoint was progress-free survival (PFS). Results The median level of SAA and CRP was 4.28 mg/L and 1.88 mg/L, respectively. For the high- SAA group (> 4.28 mg/L) versus the low-SAA ( 4.28 mg/L) group and the high-CRP group (> 1.88 mg/L) versus the low-CRP ( 1.88 mg/L) group, the 5-year PFS was 64.5% versus 73.1% (p=0.013) and 65.2% versus 73.3% (p=0.064), respectively. EBV DNA detection showed a superior predictive result, the 5-year PFS in the EBV DNA  1,500 copies/mL group was obviously different than the EBV DNA < 1,500 copies/mL group (62.2% versus 77.8%, p < 0.001). Multifactorial Cox regression analysis confirmed that in the PFS, the independent prognostic factors were including EBV DNA (hazard ratio [HR], 1.788; p=0.009), tumour stage (HR, 1.903; p=0.021), and node stage (HR, 1.498; p=0.049), but the SAA and CRP were not included in the independent prognostic factors. Conclusion The results of SAA and CRP had a certain relationship with the prognosis of NPC, and the prognosis of patients with high level of SAA and CRP were poor. However, the predictive ability of SAA and CRP was lower than that of EBV DNA.

Complete mitochondrial genome of a kind of snakehead fish Channa siamensis and its phylogenetic consideration

Rui Li,Gang Wang,Zheng‑Yong Wen,Yuan‑Chao Zou,Chuan‑Jie Qin,Yu Luo,Jun Wang,Gui‑Hong Chen 한국유전학회 2019 Genes & Genomics Vol.41 No.2

The snakehead fish, Channa siamensis, belongs to the genus of Channa (perciformes: Channidae) and was first reported by Günther in 1861. Despite it has been described approximately for 15 decades, the genetic information is limited and the taxon status of this kind of fish is still unclear. The primary objective of this study is to get more genomic data and calculate the taxon location of this kind of fish. The next generation sequencing method was used to obtain the whole mitochondrial DNA information, and bioinformatic analysis was performed to investigate the evolutionary status and taxon location of C. siamensis. The circular mitochondrial DNA was 16,570 bp in length, and which showed typical piscine structure and arrangement. The overall nucleotide composition was 29.28% A, 24.72% T, 30.71% C, 15.29% G, with 54.1% AT, respectively. Phylogenetic analyses using concatenated amino acid and nucleotide sequences of the 13 protein-coding genes with two different methods (Maximum likelihood and Bayesian analysis) both highly supported C. siamensis belongs to the genus Channa and shows a close relationship with C. micropeltes. These data will provide more useful information for a better understanding of the mitochondrial genomic diversities and evolution in fish as well as novel genetic markers for studying population genetics and species identification.

Temperature distribution prediction in longitudinal ballastless slab track with various neural network methods

Rui Zhou,Hanlin Liu,Wenhao Yuan,Yanliang Du,Jingmang Xu,Rong Chen 국제구조공학회 2023 Smart Structures and Systems, An International Jou Vol.32 No.2

The temperature prediction approaches of three important locations in an operational longitudinal slab track-bridge structure by using three typical neural network methods based on the field measuring platform of four meteorological factors and internal temperature. The measurement experiment of four meteorological factors (e.g., ambient temperature, solar radiation, wind speed, and humidity) temperature in the three locations of the longitudinal slab and base plate of three important locations (e.g., mid-span, beam end, and Wide-Narrow Joint) were conducted, and then their characteristics were analyzed, respectively. Furthermore, temperature prediction effects of three locations under five various meteorological conditions are tested by using three neural network methods, respectively, including the Artificial Neural Network (ANN), the Long Short-Term Memory (LSTM), and the Convolutional Neural Network (CNN). More importantly, the predicted effects of solar radiation in four meteorological factors could be identified with three indicators (e.g., Root Means Square Error, Mean Absolute Error, Correlation Coefficient of R2). In addition, the LSTM method shows the best performance, while the CNN method has the best prediction effect by only considering a single meteorological factor.

Complementary transcriptomic and proteomic analysis of Bombyx mori middle silk glands reveals a predominant ribosome-biogenesis regulating network during silkworm yellow-cocoon color formation

Yuan Yi,Xiao Rui,Ge Qi,Taha Rehab Hosny,Chen Keping 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

The human macular carotenoid transporter was firstly discovered using silkworm yellow-cocoon strains, known as Bombyx mori carotenoid selective transport system (BmCSTS). However, molecular mechanisms underlying the gene/protein expression and regulations in vivo remain elusive. Here, 620 differentially expressed genes (DEGs), 1965 differentially expressed proteins (DEPs), and 67 co-genes (namely Co-DEGs-DEPs) from yellowcocoon silkworm middle silk glands (MSGs) were identified by RNA-Seq based transcriptome and SWATHbased proteomic analysis. Notably, previously identified CBP-alike key factors were further demonstrated significantly up-regulated in all six B. mori yellow-cocoon strains (NB, NB × 306, 306 × NB, NB × 798, 798 × NB, and XH × NB) used in this study. The GO and KEGG analysis results clustered most DEGs and DEPs into multiple biological processes including both cellular components and molecular functions, especially protein synthesis and energy-metabolic pathways. The protein–protein interaction (PPI) network analysis results further revealed a predominant ribosome-biogenesis regulation working system during silkworm yellow-cocoon color formation. Additionally, high-efficient energy production and metabolism pathways (i.e. oxidative phosphorylation and citrate cycle) were also discovered, which might facilitate key gene/protein expressions and functions in BmCSTS, such as CBP and Cameo2.

Comparative Lipidomic Analysis of Cephalosporium acremonium Insights into Industrial and Pilot Fermentations

Rui-Juan Xu,Bin Qiao,Bing-Zhi Li,Hua Lu,Yao Chen,Ying-Jin Yuan 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.2

Cephalosporium acremonium has been widely applied in industrial cephalosporin C fermentation. However,little is known about the molecular basis of fermentation behavior of this strain. In this study, comparative lipidomic analysis using LC/ESI/MSn technology was employed to investigate responses of Cephalosporium acremonium to multiple environment variations in realistic industrial cephalosporin C fermentation process and provide molecular basis for the discrepancies between industrial and pilot fermentations. Totally 77 phospholipids species were detected and 65 species were further quantified. Score plot revealed that phospholipids metabolism differed in industrial and pilot process. Loading pilot indicated that the main variables responsible for the discrimination of industrial and pilot process were phosphatidylinositols (PIs), phosphatidylserines (PSs) and phosphatic acids (PAs). Higher PIs content in industrial process indicated that cells were more vigorous in industrial process than those in pilot process. Larger increases of PSs, PAs and ratio of oleic acid to linoleic acid coincided well with the earlier and more thorough cellular morphological differentiation in industrial process. The synergetic reaction between cellular behavior and cells living environment led to titer discrepancies between industrial and pilot process. These findings provided lipidomic insights into industrial cephalosporin C production. Cephalosporium acremonium has been widely applied in industrial cephalosporin C fermentation. However,little is known about the molecular basis of fermentation behavior of this strain. In this study, comparative lipidomic analysis using LC/ESI/MSn technology was employed to investigate responses of Cephalosporium acremonium to multiple environment variations in realistic industrial cephalosporin C fermentation process and provide molecular basis for the discrepancies between industrial and pilot fermentations. Totally 77 phospholipids species were detected and 65 species were further quantified. Score plot revealed that phospholipids metabolism differed in industrial and pilot process. Loading pilot indicated that the main variables responsible for the discrimination of industrial and pilot process were phosphatidylinositols (PIs), phosphatidylserines (PSs) and phosphatic acids (PAs). Higher PIs content in industrial process indicated that cells were more vigorous in industrial process than those in pilot process. Larger increases of PSs, PAs and ratio of oleic acid to linoleic acid coincided well with the earlier and more thorough cellular morphological differentiation in industrial process. The synergetic reaction between cellular behavior and cells living environment led to titer discrepancies between industrial and pilot process. These findings provided lipidomic insights into industrial cephalosporin C production.

Effect of High Energy Shot Peening on the Microstructure and Mechanical Property of AZ31B Mg Alloy/HSLA350 Steel Lap Joints

Rui Li,Xinjian Yuan,Ting Li,Miao Hong,Shiwei Tao,Zhuo Chen,Ge Wang 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.5

When using high energy shot peening (HESP) technology, the surface layer of the welded joints produced grain breakage and residual compressive stress. When the shot peening strength is the optimal parameter 0.10 MPa, the residual compressive stress reaches a maximum value of 74.02 MPa, dislocation density in welded joints increased significantly and the tensile shear strength of the joint was increased by 18.6% compared to the joint without HESP treatment (205 MPa), and the fracture position of the welded joint was located in the Al alloy base material, these were brought by fi ne grain strengthening and strain strengthening. When the shot peening strength was 0.05 MPa, the strength of the joint was also improved, but the fracture position of the joint was the same as that without HESP treatment, and they were all in the fusion zone. However, when the shot peening strength was further increased to 0.15 MPa, severe cracks appeared inside the joint, which deteriorates the strength of the Mg/steel joint.

Methods on improvements of the poor oral bioavailability of ginsenosides: Pre-processing, structural modifi cation, drug combination, and micro- or nano- delivery system

Qi-rui Hu,Huan Hong,Zhi-hong Zhang,Hua Feng,Ting Luo,Jing Li,Ze-yuan Deng,Fang Chen 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6

Panax ginseng Meyer is a traditional Chinese medicine that is widely used as tonic in Asia. The mainpharmacologically active components of ginseng are the dammarane-type ginsenosides, which havebeen shown to have anti-cancer, anti-inflammatory, immunoregulatory, neuroprotective, and metabolicregulatory activities. Moreover, some of ginsenosides (eg, Rh2 and Rg3) have been developed intonutraceuticals. However, the utilization of ginsenosides in clinic is restrictive due to poor permeability incells and low bioavailability in human body. Obviously, the dammarane skeleton and glycosyls of ginsenosidesare responsible for these limitations. Therefore, improving the oral bioavailability of ginsenosideshas become a pressing issue. Here, based on the structures of ginsenosides, we summarized theunderstanding of the factors affecting the oral bioavailability of ginsenosides, introduced the methods toenhance the oral bioavailability and proposed the future perspectives on improving the oral bioavailabilityof ginsenosides.

The ways for ginsenoside Rh2 to fight against cancer: the molecular evidences in vitro and in vivo.

Qi-rui Hu,Yao Pan,Han-cheng Wu,Zhen-zhen Dai,Qing-xin Huang,Ting Luo,Jing Li,Ze-yuan Deng,Fang Chen The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.2

Cancer is a global public health issue that becomes the second primary cause of death globally. Considering the side effects of radio- or chemo-therapy, natural phytochemicals are promising alternatives for therapeutic interventions to alleviate the side effects and complications. Ginsenoside Rh2 (GRh2) is the main phytochemical extracted from Panax ginseng C.A. Meyer with anticancer activity. GRh2 could induce apoptosis and autophagy of cancer cells and inhibit proliferation, metastasis, invasion, and angiogenesis in vitro and in vivo. In addition, GRh2 could be used as an adjuvant to chemotherapeutics to enhance the anticancer effect and reverse the adverse effects. Here we summarized the understanding of the molecular mechanisms underlying the anticancer effects of GRh2 and proposed future directions to promote the development and application of GRh2.

Courage Store II

( Lin Rui-lin ),( He Xin-hua ),( Chen Guan-ting ),( Chen Jia-ling ),( Jua Wei-ting ),( Lin Yen-chun ),( Yuan Zheng-xing ),( Lin Yi-ru ) 한국기초조형학회 2018 한국기초조형학회 작품집 Vol.2018 No.2