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Cytotoxic Activity from Curcuma zedoaria Through Mitochondrial Activation on Ovarian Cancer Cells
Shin, Yujin,Lee, Yongkyu Korean Society of ToxicologyKorea Environmental Mu 2013 Toxicological Research Vol.29 No.4
${\alpha}$-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the mechanism of the apoptotic effect of ${\alpha}$-curcumene on the growth of human overian cancer, SiHa cells. Upon treatment with ${\alpha}$-curcumene, cell viability of SiHa cells was inhibited > 73% for 48 h incubation. ${\alpha}$-Curcumene treatment showed a characteristic nucleosomal DNA fragmentation pattern and the percentage of sub-diploid cells was increased in a concentration-dependent manner, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of ${\alpha}$-curcumene, which mediates cell death. These results suggest that the apoptotic effect of ${\alpha}$-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.
Cytotoxic Activity from Curcuma zedoaria Through Mitochondrial Activation on Ovarian Cancer Cells
Yujin Shin,Yongkyu Lee 한국독성학회 2013 Toxicological Research Vol.29 No.4
α-Curcumene is one of the physiologically active components of Curcuma zedoaria, which is believed to perform anti-tumor activities, the mechanisms of which are poorly understood. In the present study, we investigated the mechanism of the apoptotic effect of α-curcumene on the growth of human overian cancer, SiHa cells. Upon treatment with α-curcumene, cell viability of SiHa cells was inhibited > 73% for 48 h incubation. α-Curcumene treatment showed a characteristic nucleosomal DNA fragmentation pattern and the percentage of sub-diploid cells was increased in a concentration-dependent manner, hallmark features of apoptosis. Mitochondrial cytochrome c activation and an in vitro caspase-3 activity assay demonstrated that the activation of caspases accompanies the apoptotic effect of α-curcumene, which mediates cell death. These results suggest that the apoptotic effect of α-curcumene on SiHa cells may converge caspase-3 activation through the release of mitochondrial cytochrome c.
Food, Body, and Prostitution in Christopher Marlowe’s The Jew of Malta
Yujin Shin Ewha Institute of English and American Studies 2019 Journal of English and American studies Vol.17 No.1
In this paper, I will mainly focus on the signification of Barabas’s evil deeds in Christopher Marlowe’s The Jew of Malta. Once Barabas, a master of commercialism realizes the unstable nature of money, and thus realizes that money cannot guarantee absolute power, his object of concern changes to the human body, with which he can manipulate. I have analyzed how the body and the food are used by Barabas to suggest that his misdeeds reflect those of the exploiters in the world of commercial sex. Treating the body like food, both Barabas and the consumer of prostitution consume the body for their pleasure; just as the consumer reduces the use-value of the prostitute’s body, Barabas sucks the life out of other bodies. And both Barabas and the pimp uses the body to allure other consumers; just as the pimp uses a prostitute as fish bait to allure other customers, Barabas also uses his daughter, Abigail, to allure other characters. Yet the play warns against Barabas’s behavior. Barabas’s tendency to exchange food for a body indicates that such an exchange will have a detrimental effect upon the community. Food stands as a typical symbol of social bondage, and abandoning food for a body means that Barabas’s perpetual pursuit of bodies will eventually disintegrate the community. Thus, excessive covetousness displayed by the consumers and pimps in prostitution poses a risk to communal ties also. The play does not end by giving a warning, but by punishing the exploiter. Barabas dies by becoming a mere food in a boiling pot. This ending implies that the exploiter may face his own demise by becoming the very victim he has been exploiting.
전복(Haliotis discus)에서 분리한 Serratia marcescens가 생산하는 적색 색소의 항균활성
신유진(YuJin Shin),강창호(Chang-Ho Kang),소재성(Jae-Seong So) 한국생물공학회 2016 KSBB Journal Vol.31 No.4
Serratia marcescens characterized by the ability to produce red pigments inhabits various ecological niches. A strain Serratia marcescens PYU was isolated from abalone (Haliotis discus) collected at the West Sea in Korea. The isolated strain was gram-negative, motile, rods like coccus, oxidase-negative, and catalase-positive; and formed red pigment. S. marcescens PYU was grown in the presence of 0~10% (w/v) NaCl, at pH 4~9, and at 10~40°C. The strain PYU produced red pigment, and the extracted pigment showed antibacterial activity against Streptococcus iniae and Lactococcus garviae which has been known as an important fish pathogens. Further studies are underway to elucidate the direct relationship between the red pigment and antibacterial activity.
Kweon Yujin,Fang Miao,Shin Sang-Yoon,Lee Dowhan,Kim Kook-Hyung,Shin Chanseok 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.6
Double-stranded RNA (dsRNA)-induced RNA interference is a promising agricultural technology for crop protection against various pathogens. Recent advances in this field have enhanced the overall efficiency with which this approach inhibits pathogenic viruses. Our previous study verified that treatment of Nicotiana benthamiana plants with dsRNAs targeting helper component-proteinase (HC-Pro) and nuclear inclusion b (NIb) genes protected the plant from pepper mottle virus (PepMoV) infection. The aim of this study was to improve the inhibitory efficacy of dsRNAs by optimizing the target sequences and their length and by targeting multiple genes via co-treatment of dsRNAs. Each of the two targeting dsRNAs were divided into three shorter compartments and we found that HC-Pro:mid- 1st and NIb:mid-3rd showed significantly superior antiviral potency than the other fragments, including the parent dsRNA. In addition, we confirmed that the co-treatment of two dsRNAs targeting HC-Pro and NIb produced a greater inhibition of PepMoV replication than that obtained from individual dsRNA treatment. Complementing our previous study, this study will provide future directions for designing dsRNAs and enhancing their efficiency in dsRNA-mediated RNA interference technologies. Double-stranded RNA (dsRNA)-induced RNA interference is a promising agricultural technology for crop protection against various pathogens. Recent advances in this field have enhanced the overall efficiency with which this approach inhibits pathogenic viruses. Our previous study verified that treatment of Nicotiana benthamiana plants with dsRNAs targeting helper component-proteinase ( HC-Pro ) and nuclear inclusion b ( NIb ) genes protected the plant from pepper mottle virus (PepMoV) infection. The aim of this study was to improve the inhibitory efficacy of dsRNAs by optimizing the target sequences and their length and by targeting multiple genes via co-treatment of dsRNAs. Each of the two targeting dsRNAs were divided into three shorter compartments and we found that HC-Pro :mid-1st and NIb :mid-3rd showed significantly superior antiviral potency than the other fragments, including the parent dsRNA. In addition, we confirmed that the co-treatment of two dsRNAs targeting HC-Pro and NIb produced a greater inhibition of PepMoV replication than that obtained from individual dsRNA treatment. Complementing our previous study, this study will provide future directions for designing dsRNAs and enhancing their efficiency in dsRNA-mediated RNA interference technologies.