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Pengjie Wang,Chuan Yue,Di Chen,Yucheng Zheng,Qian Zhang,Jiangfan Yang,Naixing Ye 한국유전학회 2019 Genes & Genomics Vol.41 No.1
The WRKY transcription factors (TFs) family is one of the largest TF families in plants and plays a central role in diverse regulation and multiple stress responses. However, the systematical analysis of the WRKY gene family in tea plant (Camellia sinensis) based on genomic data has been lacking. The primary objective of this study was to set a systematic analysis of the WRKY gene family based on genomic data in tea plant and analyze their expression profiles under various abiotic stresses. We searched the tea plant genome using the consensus model of the WRKY domain (PF03106) and then used these search results to identify all the WRKY family members by SMART and the CDD program. Analyze their phylogeny, classification, structure, conserved motifs, Cis-elements, interactors and expression profiles. 56 putative WRKY genes were identified from the tea plant genome and divided into three main groups (I–III) and five subgroups (IIa–IIe) according to the WRKY domains and the zinc-finger structure. The gene structure and conserved motifs of the CsWRKY genes were also characterized and were consistent with the classification results. Annotation analysis showed that 34 CsWRKY genes may be involved in stress responses. Promoter analysis implied that CsWRKY genes, except for CsWRKY55, possessed at least one abiotic stress response cis-element. Expression profiles of CsWRKY genes in different tissues were analyzed with RNA-seq data. The results showed that 56 CsWRKY genes had differential expression in their transcript abundance. The expression profiles also showed that many identified CsWRKY genes were possibly involved in the response to cold, drought, salt, or ABA treatment. Tea plant genome contains at least 56 WRKY genes. These results provide useful information for further exploring the function and regulatory mechanism of CsWRKY genes in the growth, development, and adaption to abiotic stresses in tea plant.
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Bo Long,Wei Wang,Xin Lu,YuFei Dai,LiJun Huang,YuCheng Qian,Yong Chen,FuSheng Li 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5
Conventional phase-shift controlled dual active bridge (DAB) converters which operate in discontinuous current mode (DCM) are hard to realize the zero-voltage switching (ZVS) due to the light load. This paper proposed an efficiency improvement method for the DAB converter with fix-frequency hybrid discontinue current mode (HDCM). By adopting the HDCM control method, synchronous rectification and wide load range ZVS of switching devices are realized simultaneously without changing the components of the conventional DAB power stage in DCM. Besides, to further improve the conversion ratio and efficiency, the voltage-doubled rectification (VDR) is adopted. The HDCM implementation method with a digital signal processor (DSP) is proposed. A 200W laboratory prototype is designed to validate the correctness of HDCM. Compared to the conventional DAB converter which operates in DCM, experimental results demonstrate that the efficiency was increased 1.2% at 10% rated load.
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