Excessive mechanical strain accelerates intervertebral disc degeneration by disrupting intrinsic circadian rhythm

Ding Sheng-Long,Zhang Tai-Wei,Zhang Qi-Chen,Ding Wang,Li Ze-Fang,Han Guan-Jie,Bai Jin-Song,Li Xi-Lei,Dong Jian,Wang Hui-Ren,Jiang Li-Bo 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Night shift workers with disordered rhythmic mechanical loading are more prone to intervertebral disc degeneration (IDD). Our results showed that circadian rhythm (CR) was dampened in degenerated and aged NP cells. Long-term environmental CR disruption promoted IDD in rats. Excessive mechanical strain disrupted the CR and inhibited the expression of core clock proteins. The inhibitory effect of mechanical loading on the expression of extracellular matrix genes could be reversed by BMAL1 overexpression in NP cells. The Rho/ROCK pathway was demonstrated to mediate the effect of mechanical stimulation on CR. Prolonged mechanical loading for 12 months affected intrinsic CR genes and induced IDD in a model of upright posture in a normal environment. Unexpectedly, mechanical loading further accelerated the IDD in an Light-Dark (LD) cycle-disrupted environment. These results indicated that intrinsic CR disruption might be a mechanism involved in overloading-induced IDD and a potential drug target for night shift workers.

Changes in Markers of Liver Function in HCV 1b Patients with Compensated Cirrhosis Treated with Ombitasvir/Paritaprevir/ Ritonavir plus Dasabuvir with Ribavirin

( Jeong Heo ),( Yan Luo ),( Wan-long Chuang ),( Jidong Jia ),( Kwang-hyub Han ),( Ming-lung Yu ),( Hong Tang ),( Young-suk Lim ),( Cheng-yuan Peng ),( Min Xu ),( Maorong Wang ),( Bo Fu ),( Niloufar Mo 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: Patients chronically infected with HCV are at risk of developing extrahepatic manifestations of HCV as well as progressing to compensated or decompensated cirrhosis and HCC. Although current treatments have high rates of SVR, relatively little is known about possible regression of liver fibrosis after achieving an SVR. The ONYX-II trial examined the efficacy and safety of ombitasvir/paritaprevir/ritonavir plus dasabuvir + ribavirin (RBV) in Asian patients with HCV genotype 1b infection and compensated cirrhosis. Here we report changes in key markers of liver fibrosis and function. Methods: Patients with chronic HCV GT1b infection and compensated cirrhosis were enrolled in China, South Korea and Taiwan and received 12 weeks of OBV/PTV/r (25 mg/150 mg/100 mg once daily) and DSV (250 mg twice daily) with weight-based RBV. The primary objective of ONYX-II was to assess efficacy (SVR12) and safety of the regimen. Changes in markers of liver fibrosis and function between baseline (BL) and post-treatment week (PTW) 12 are presented. Results: Overall, 104 patients were enrolled and treated in ONYX-II. All patients (104/104, 100%) achieved SVR12. BL and PTW12 data for FibroTest score, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin, albumin, platelet count and alpha fetoprotein (AFP) are shown in Table 1. All selected parameters showed numerical improvements between BL and PTW12. Mean ALT and AST levels returned to within normal range and FibroTest scores demonstrated a numerical improvement, suggesting improvement in liver status. The complete set of data between BL and PTW12 will be presented for these parameters and other liver composite parameters at the conference. Conclusions: Measurement of key liver function markers during the ONYX-II trial showed a numerical improvement within 12 weeks of completion of treatment in HCV GT1b-infected patients with compensated cirrhosis. Further follow-up of these patients will determine the long-term durability of these changes.

Numerator-Denominator Model Based H<SUB>∞</SUB> Robust Control for LCL Filter-Based Grid-Connected Inverters under Weak Grid Conditions

Bo Long,Chu Wang,ZiLin Zhu,Xin Lu,LiJun Huang,YuFei Dai,Yong Liao,FuSheng Li 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

In the grid-connected inverter controller system, conventional PI or PR controller decrease their lowfrequency gain and bandwidth to maintain the stability of the system. However, this degrades the disturbance rejection and current tracking capability. To solve this problem, this paper gives an H∞ robust control scheme based on Numerator Denominator Model method, which has the advantages of simplified current controller design procedure, can place the poles of closed-loop system at the desired place. By properly selecting the weight function, the proposed control scheme can not only achieve good robustness in disturbance rejection capability, but also gives a straight forward to design the dynamic performance. Simulation and experimental comparison results between PR and H∞ controller is demonstrated. The results prove that the proposed control scheme shows obviously improvements on the robustness of inverter and meanwhile does not lose advantages on the dynamic performance.

miR-23a Regulates Cardiomyocyte Apoptosis by Targeting Manganese Superoxide Dismutase

Bo Long,Tian-Yi Gan,Rong-Cheng Zhang,Yu-Hui Zhang 한국분자세포생물학회 2017 Molecules and cells Vol.40 No.8

Cardiomyocyte apoptosis is initiated by various cellular insults and accumulated cardiomyocyte apoptosis leads to the pathogenesis of heart failure. Excessive reactive oxygen species (ROS) provoke apoptotic cascades. Manganese superoxide dismutase (MnSOD) is an important antioxidant enzyme that converts cellular ROS into harmless products. In this study, we demonstrate that MnSOD is down-regulated upon hydrogen peroxide treatment or ischemia/reperfusion (I/R) injury. Enhanced expression of MnSOD attenuates cardiomyocyte apoptosis and myocardial infarction induced by I/R injury. Further, we show that miR-23a directly regulates the expression of MnSOD. miR-23a regulates cardiomyocyte apoptosis by suppressing the expression of MnSOD. Our study reveals a novel model regulating cardiomyocyte apoptosis which is composed of miR-23a and MnSOD. Our study provides a new method to tackling apoptosis related cardiac diseases.

SIMULATION OF MIXED GAS FORMATION FOR A SPRAY-WALL COMPLEX GUIDED LPG DIRECT INJECTION ENGINE

Bo-yan Xu,Xiang-long Liu,Long-long Jiang,Juan Xu 한국자동차공학회 2017 International journal of automotive technology Vol.18 No.3

To obtain an ultralean air-fuel ratio and to reduce engine-out NOX and HC emissions induced by the richer mixture near the spark plug, a spray and wall complex guided combustion system has been developed by utilizing the fuel characteristics of LPG. The new combustion system configuration is optimized by using a commercial CFD code, FIRE V2013, and the reliability of the system has been experimentally demonstrated by Plane Laser-Induced Fluorescence (PLIF). The mixture formation in the new combustion system under part load (2,000 rpm) is numerically simulated. With an injection timing of 40°CA BTDC, the LPG spray which is injected from two upper holes, reaches the ignition point, and the other part of the LPG spray which is injected from the bottom hole, is directed to the ignition point through the vertical vortices at the same time. At the ignition timing of about 20°CA BTDC, the two-part mixtures have been shown to form a stable and richer stratified mixture around the ignition point, and the maximum global air-fuel ratio reaches to 60 : 1.

miR-23a Regulates Cardiomyocyte Apoptosis by Targeting Manganese Superoxide Dismutase

Long, Bo,Gan, Tian-Yi,Zhang, Rong-Cheng,Zhang, Yu-Hui Korean Society for Molecular and Cellular Biology 2017 Molecules and cells Vol.40 No.8

Cardiomyocyte apoptosis is initiated by various cellular insults and accumulated cardiomyocyte apoptosis leads to the pathogenesis of heart failure. Excessive reactive oxygen species (ROS) provoke apoptotic cascades. Manganese superoxide dismutase (MnSOD) is an important antioxidant enzyme that converts cellular ROS into harmless products. In this study, we demonstrate that MnSOD is down-regulated upon hydrogen peroxide treatment or ischemia/reperfusion (I/R) injury. Enhanced expression of MnSOD attenuates cardiomyocyte apoptosis and myocardial infarction induced by I/R injury. Further, we show that miR-23a directly regulates the expression of MnSOD. miR-23a regulates cardiomyocyte apoptosis by suppressing the expression of MnSOD. Our study reveals a novel model regulating cardiomyocyte apoptosis which is composed of miR-23a and MnSOD. Our study provides a new method to tackling apoptosis related cardiac diseases.

Parameter Design and Power Flow Control of Energy Recovery Power Accumulator Battery Pack Testing System

Long Bo,Kil To Chong 대한전기학회 2013 Journal of Electrical Engineering & Technology Vol.8 No.4

This paper proposes a special power circuit topology and its corresponding control strategy for an energy recovery power accumulator battery pack testing system (PABPTS), which is particularly used in electric vehicles. Firstly, operation principle and related parameter design for the system are illustrated. Secondly, control strategy of the composite power converter for PABPTS is analyzed in detail. The improved scheme includes a high accuracy charge and discharge current closed loop. active power reference for the grid-side inverter is provided by the result of multiplication between battery pack terminal voltage and test current. Simulation and experimental results demonstrate that the proposed scheme could not only satisfy the requirements for PABPTS with widerange current test, but also could recover the discharging energy to the power grid with high efficiency.

Light Load Efficiency Improvement Method for GaN-Based DAB Converter with Hybrid Discontinuous Current Mode

Bo Long,Wei Wang,Xin Lu,YuFei Dai,LiJun Huang,YuCheng Qian,Yong Chen,FuSheng Li 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

Conventional phase-shift controlled dual active bridge (DAB) converters which operate in discontinuous current mode (DCM) are hard to realize the zero-voltage switching (ZVS) due to the light load. This paper proposed an efficiency improvement method for the DAB converter with fix-frequency hybrid discontinue current mode (HDCM). By adopting the HDCM control method, synchronous rectification and wide load range ZVS of switching devices are realized simultaneously without changing the components of the conventional DAB power stage in DCM. Besides, to further improve the conversion ratio and efficiency, the voltage-doubled rectification (VDR) is adopted. The HDCM implementation method with a digital signal processor (DSP) is proposed. A 200W laboratory prototype is designed to validate the correctness of HDCM. Compared to the conventional DAB converter which operates in DCM, experimental results demonstrate that the efficiency was increased 1.2% at 10% rated load.

DC component Mitigation Utilizing BP-PI and Repetitive Control in Transformless Grid-Connected Inverters

Bo Long,Yong Liao,YuFei Dai,LiJun Huang,Xin Lu,Yong Chen,FuSheng Li 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

Transformerless grid-connected inverters, due to their advantages in high efficiency, small volume and light weight, gain more research and interest in recent years. Due to the asymmetrical driving signal in pulse-width-modulation (PWM) caused by time-delay, zero-drift of the current sensors and imparities of the power transistors, etc. Output of the grid current contains dc component. As a result, power quality of the grid is degraded. In this paper, a dc component suppression scheme with recursive integral PI controller are introduced, according to the change tendency of tracking error, the coefficients of PI controller are regulated by BP neural network in real time, and the global optimization requirements are met. Sliding-Window-Double-Iteration-Method (SWDIM) is utilized for fast dc current extraction. Simulation results from a 2-kW three-phase grid-connected inverter confirm the effectiveness of the proposed scheme.

Parameter Design and Power Flow Control of Energy Recovery Power Accumulator Battery Pack Testing System

Bo, Long,Chong, Kil To The Korean Institute of Electrical Engineers 2013 Journal of Electrical Engineering & Technology Vol.8 No.4

This paper proposes a special power circuit topology and its corresponding control strategy for an energy recovery power accumulator battery pack testing system (PABPTS), which is particularly used in electric vehicles. Firstly, operation principle and related parameter design for the system are illustrated. Secondly, control strategy of the composite power converter for PABPTS is analyzed in detail. The improved scheme includes a high accuracy charge and discharge current closed loop. active power reference for the grid-side inverter is provided by the result of multiplication between battery pack terminal voltage and test current. Simulation and experimental results demonstrate that the proposed scheme could not only satisfy the requirements for PABPTS with wide-range current test, but also could recover the discharging energy to the power grid with high efficiency.