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이현철,유호종,김진섭,함성호,신장규,이종현,이정희,권성원,박세일 경북대학교 센서기술연구소 1997 센서技術學術大會論文集 Vol.8 No.1
A planar Bi-Sb multijunction thermal converter for the precise measurements of ac voltage and current has been fabricated and its characteristics was discussed. In order to convert ac power into heat, a bifilar thin film Pt-heater, which could cancel its Thomson and Peltier effects, was prepared on the Si_(3)N_(4)/SiO_(2)/Si_(3)N_(4) diaphragm for the thermal isolation between heater and silicon substrate. To convert the temperature or the heat generated by the heater into dc voltage and current, hot and cold junctions of the Bi-Sb thermopile, which has a large difference in Seebeck coefficients, were formed on the dielectric diaphragm and the silicon substrate, respectively. The respective thermal sensitivity of the thermal converter with a bifilar heater was about 10.1 mV/mW and 14.8 mV/mW in the air and vacuum, which is about eight times higher than that of commercial 3-dimensional thermal converter. In the case of ac 2 V-input voltage, the ac-dc voltage transfer difference was about ±2.0 ppm, and in the case of ac 10 mA-input current, the ac-dc current transfer difference was about ± 0.6 ppm, in the frequency range from 10 Hz to 10 kHz.
Bacillus lentimorbus WJ5의 감마선유도 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자의 microarray 분석
이영근,장화형,장유신,허재호,형석원,정혜영 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.3
본 연구에서는 감마선으로 유도된 돌연변이체들에서 공통으로 발현되는 방사선 관련 유전자들의 발현을 연구하기 위하여, B. lentimorbus WJ5의 방사선 유도 돌연변이체에서 발현되는 유전자를 DNA microarray로 동시에 탐색하였다. DNA microarray는 B. lentimorbus WJ5 genome을 무작위로 절단하여 2,000단편으로 구성하였으며, 감마선 (^(60)Co)으로 유도된 7돌연변이체의 발현을 정량적으로 관찰하였다. 클러스터 분석결과 발현된 408 유전자 중 27개가 감마선 유도 돌연변이체 모두에서 유의하게 발현이 증가되었다. 특히, 복구(mutL, mutM)에너지 대사 (acsA, sdhB, p하, yhjB, citB), protease(npr), 산화자극에 대한 환원 (HMM)관련 유전자들이 동시에 증가되었다. 이는 감마선 유도 돌연변이체들에서 자발적인 직/간접 복구 관련 유전자의 발현 증가는 방사선 노출 직후 보이는 stress response와는 다른 현상임을 나타내는 것으로 생각된다. To study the radiation related gene expression in mutants of Bacillus lentimorbus WJ5 induced by gamm radiation, the simultaneous gene expression was analyzed by DNA microarray. We constructed DNA chips including two thousand randomly digested genome spots of B. lentimorbus WJ5 and compared its quantitative aspect with seven mutants induced by gamma radiation (^(60)Co). From the cluster analysis of gene expression pattern, totally 408 genes were expressed and 27 genes were significantly upregulated by the gamma radiation in all mutants. Especially, genes involved in repair (mutL, mutM), energy metabolism (acsA, sdhB, pgk, yhjB, citB), protease (npr), and reduction response to oxidative stress (HMM) were simultaneously upregulated. It seems that the induction of the direct and/or indirect repair related genes in mutants induced by gamma radiation could be remarkably different from the adaptive responses against acute exposure to radiation.
Bacillus sp. MS202에 의한 Dinitroaniline계 제초제인 Pendimethalin의 부분환원
이영근,장화형,장유신,형석원,정혜영 한국환경농학회 2004 한국환경농학회지 Vol.23 No.4
토양과 지하수에서 pendimethalin의 지속성은 환경에 해로운 영향을 미친다. 경남 마산에서 분리한 pendimethalin분해 균주는 API CHB50 kit 시험, FAME 분석, 그리고 165 rDNA 염기서열분석 결과로 Bacillus sp. MS202로 잠정적으로 동정하였다. TLC, GC, 그리고 GC-MS 분석에 의해 Bacillus sp. MS202가 pendimethalin의 -NO₂를 -NH₂로 환원시킨다는 것을 알 수 있었다. 이는 일반적으로 알려진 호기성 미생물에 의한 pendimethalin 분해가 탈알킬화가 우선한다는 보고와 상반되는 새로운 결과이다. The persistence of pendimethalin in soil and ground water has an injurious effect on ecosystem. Pendimethalin-degrading bacterium was isolated from Masan, Gyeongnam province and temporarily identified as Bacillus sp. MS202 by the analysis of API CHB50, kit, FAME, and 16S rDNA sequence. From the analysis of pendimethalin metabolite using TLC, GC, and GC-MS, we found that the degradation of pendimethalin by Bacillus sp. MS202 did not result in the dealkylated form, but the formation of the reduced compound, 6-amino-2-nitro-N(1-ethylpropyl)-3,4-xylidine or 2-amino-6-nitro-N(1-ethylpropyl)-3,4-xylidine.
이차원전기영동법을 이용한 길항세균 Bacillus licheniformis DM3와 이의 항진균 활성 결여 돌연변이균주간 단백질 비교 분석
이영근,김재성,정혜영,장유신,장병일 한국환경농학회 2003 한국환경농학회지 Vol.22 No.3
항진균 활성균주를 선발하는 과정에서 DM3 균주를 대천 바닷가에서 수집된 진흙 시료로부터 분리하였으며 API 50CHB kit를 이용하여 동정한 결과 Bacillus licheniformis로 동정되었다. 이 균주는 12종의 식물병원성 진균에 대해 항균활성을 나타내었다. 감마선(^(60)Co)을 조사하여 항진균 활성 결핍 돌연변이체(mDM3)를 유도한 후, 이차원전기영동으로 단백질을 분석한 결과 DM3와 mDM3에만 존재하는 각각 4종과 3종의 단백질을 확인할 수 있었다. 2-DE 결과 B. licheniformis DM3에서 spot 1은 serine hydroxymethyltransferase(45.0 kDa), spot 2는 hypothetical protein(40.7 kDa), spot 3는 NifU protein homolog(15.4 kDa), 그리고 spot 4는 resolvase(12.5 kDa)와 상동성을 지닌 단백질로 동정되었고 B. licheniformis mDM3에서만 발현된 spot 5는 lysozyme(18.1 kDa)과 spot 6, 7은 alkyl hydroperoxide reductase(15.6 kDa)으로 동정되었다. B. licheniformis DM3에서 이들 단백질들의 항진균 활성 관련 여부를 규명하기 위해서 더 연구가 진행되어야 할 것으로 사료된다. In the course of screening for antifungal agents, a bacterial strain, DM3 was isolated from a mud sample collected at Daechon in Chungnam province and identified as Bacillus lichenifomris based on API 50CHB test. It has antifurgal activity against 12 plant pathogenic fungi in paper disc assay. At the 95% lethal dose of gamma radiation (^(60)Co, 10 kGy, D_(10)=2.32 kGy), the antifungal activity deficient mutant (mDM3) against Colletotrichum gloeosporioides was induced. From 2-D electrophoresis analysis, serine hydroxymethyltransferase(45.0 kDa), hypothetical protein(40.7 kDa), NifU protein homolog(l5.4 kDa), and resolvase(l2.5 kDa) horn ologous proteins were detected only in B. lichnifomris DM3. Lysozyme(l8.1 kDa) and dkyl hydroperoxide reductase(l5.6 kDa) homologous proteins were expressed uniquely in B. licheniformis mDM3. Further studies are needed to reveal that these proteins from B. lichenifomis DM3 could be closely related to the antifungal activity against plant pathogenic fungi.
Construction and Characterization of Shuttle Vectors for Succinic Acid-Producing Rumen Bacteria
Jang, Yu-Sin,Jung, Young Ryul,Lee, Sang Yup,Kim, Ji Mahn,Lee, Jeong Wook,Oh, Doo-Byoung,Kang, Hyun Ah,Kwon, Ohsuk,Jang, Seh Hee,Song, Hyohak,Lee, Sang Jun,Kang, Kyu Young American Society for Microbiology 2007 Applied and environmental microbiology Vol.73 No.17
<B>ABSTRACT</B><P>Shuttle vectors carrying the origins of replication that function in <I>Escherichia coli</I> and two capnophilic rumen bacteria, <I>Mannheimia succiniciproducens</I> and <I>Actinobacillus succinogenes</I>, were constructed. These vectors were found to be present at ca. 10 copies per cell. They were found to be stably maintained in rumen bacteria during the serial subcultures in the absence of antibiotic pressure for 216 generations. By optimizing the electroporation condition, the transformation efficiencies of 3.0 × 10<SUP>6</SUP> and 7.1 × 10<SUP>6</SUP> transformants/μg DNA were obtained with <I>M. succiniciproducens</I> and <I>A. succinogenes</I>, respectively. A 1.7-kb minimal replicon was identified that consists of the <I>rep</I> gene, four iterons, A+T-rich regions, and a <I>dnaA</I> box. It was found that the shuttle vector replicates via the theta mode, which was confirmed by sequence analysis and Southern hybridization. These shuttle vectors were found to be suitable as expression vectors as the homologous <I>fumC</I> gene encoding fumarase and the heterologous genes encoding green fluorescence protein and red fluorescence protein could be expressed successfully. Thus, the shuttle vectors developed in this study should be useful for genetic and metabolic engineering of succinic acid-producing rumen bacteria.</P>