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      • 유전자 증폭법(PCR)을 이용한 methicillin-registant Staphylococcus 균속의 신속동정에 관한 연구

        김영권 ; 장철수 ; 김병원 ; 박영진 김천대학교 2000 김천대학교 논문집 Vol.21 No.-

        In order to indentify rapidly the methicillin-resistant genus Staphylococcus (MRS) which is known as a causative bacteria of nosocomial infection, we performed the polymerase chain reaction (PCR) method. In this study, we used 44 strains which were consist of 39 starins of Staphylococcus aureus, 2 strains of Staphylococcus epidermidis. 1 strain of Staphylococcus warneri, 1 strain of Staphylococcus cohnii isolated from clinical specimens and 1 type strain of Staphylococcus aureus KCTC 1928 for PCR experiments. DNA was extracted directly from the bacterial colony. 22 mer-oligonucleotides were used as primer which complements to the mecA region of structural gene coding low affinity binding pro-tein (PBP2a). A single band of 533 bp length from MRSA, MRSE and MRSW were confirmed. Also, an antibiotic susceptibility test by agar dilution method of NCCLS was performed for the strains isolat-ed from clinical specimens. The results obtained by this method were compared with the PCR results. In the result, most of the strains which have strong resistance to methicillin were amplified for the 533 bp fragment of the mecA gene in PCR experiment. However, 4 strains (MRA1-10, MRW2-3. MRE1-15. MRE1-16) that were classified as a sensitive group in the antibiotic susceptibility test also was showed mecA gene fragment in PCR. This suggested that the specificity of mecA gene is not always consistant with that of MRSA. In order to confirm the fragment in a region of mecA gene, a southern blot hybridization process was performed using the PCR products as probe. As a result, a single hybrid band was detected at about 4 Kb region of Hind III and Hae III-digested DNA fragment in MRS. Conclusively for the diagnosis of MRS, the confirmation of mecA gene by PCR seems to be more accurate and rapid than the antibiot-ic sensitivity test.

      • 조직구성 수성망상증 2예

        최연수,박일,죄희충,김원식,양태영,허경,안기완,정춘해 조선대학교 1993 The Medical Journal of Chosun University Vol.18 No.2

        Histiocytic medullary reticulosis (HMR) is a malignant noeplastic process, with tumor cells belonging to the histiomonocytic line, and originally described by Scott and Robb-Smith in 1939 characterized by its acute onset, progression to death within a few months and its clinical features are fever, lymphadenopathy, hepatosplenomegaly, jaundice, and purpura common laboratory findings are characterized by severe anemia, leukopenia, thrombocytopenia and histologically by systemic progressive invasion of morphologically atypical histiocytes or of their precursor. 61 and 37-year-old males were admitted with camplaints of high fever and chilling sensation and were treated by cyclophosphamide, vincristine, prednison and antiobiotics, but patients all died 17 and 14 days after admission due to probably sepsis and respiratory failure.

      • Auger 電子分光에 의한 Ni-Cu, Cu-Ag 表面의 附着硏究

        朴東秀,金裕榮,李元植 慶北大學校 産業開發硏究所 1980 硏究報告 Vol.8 No.-

        Adhesion for various metals Ni-Cu-Ag after friction was measured in high vacuum chamber. Surfaces were examined with Auger-Electron-Spectroscopy in∼10_-10 torr before and after contact to determine nature of surface changes resulting from adhesive contact. Surfaces were cleaned by standard process and electrical heating for friction and AES studies. Thus, from the experimental results, it should be possible to predict which of two surfaces in contact will undergo attrition. We can see that adhesion depends on elastic moduli, surface energy, heat of sublimation, melting point and cohesive strength.

      • KCI등재후보

        간척지 중염답에서 유수형성기 염처리에 따른 벼 생육 및 수량 변화

        최원영,이규성,고종철,김상수,김태수 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.3

        본 연구는 새계화벼를 공시하여 간척지 중염답(0.3~0.4%)에서 관개수 염농도에 따른 생육 및 수량성을 검토하고자 유수형성기에 5일간 염수를 처리하여 시험한 결과를 요약하면 다음과 같다. 1. 관개수 염분농도가 높을수록 벼 출수는 1~2일 지연되었고, 출수기 지상부건물중은 가벼워졌다. 2. 간장과 수장은 관개수 염분농도가 높을수록 짧아졌다. 3. 관개수 염분농도가 높을수록 수당 립수가 적어 ㎡당 립수가 적었으며, 등숙비율이 낮고 현미 천립중이 가벼워, 쌀 수량은 민물 관개(무처리)의 330㎏/10a에 비해 관개수 염분농도 0.1%는 94%, 0.3%는 85%, 0.5%는 76%, 0.7%는 71%의 수량을 얻었다. 4. 관개수 염분농도가 높을수록 완전립 비율은 낮아졌다. 따라서 간척지에서 중염도 토양(0.3~0.4%)에서 벼를 재배하여 유수형성기에 한발이 닥쳐 저류지 등 염분 농도 0.7%의 물로 5일간 담수하여도 쌀 수량은 29% 정도 감수되고 쌀의 품위도 저하되지만 어느 정도 수량은 얻을 수 있을 것으로 생각된다. This experiment was conducted at the Kyehwado Substation of the National Honam Agricultural Experiment Station(NHAES) RDA, Korea. The experimental field contained 0.38% NaCl in soil solution. The experiment was involved five treatments (control, 0.1, 0.3, 0.5, 0.7%) in a randomized complete block design with three replicates, saline solution was applied only one time at panicle formation stage for 5 days. Saegyehwabyeo, a japonica rice variety was used in this experiment. In yield components, Spikelets number per ㎡ decreased with increasing salinity level, particularly in the 0.7% of saline solution water. This factor affected the most yield reduction among the components. The percentage of ripened grain was inclined to decrease with increasing salinity level. 1,000 grain weight decreased with increasing salinity level but it was lest affected by salinity among yield components. The reduction of milled rice yield decreased significantly with increased saline water level, in detail 6% of yield reduction at the 0.1% saline solution, 15% at the 0.3%, 24% at the 0.5%, and 29% at the 0.7% saline solution level compared with control respectively. Results indicate that the gaining of rice yield could be reliable performance in terms of economical benefit for rice production even though high reduction of yield occurred at high salinity levels on a reclaimed saline soil in Korea.

      • 이온빔 보조증착 방법으로 제작된 Al 박막의 특성

        손영호,김인수,황도원 경운대학교 산업기술연구소 1999 産業技術硏究論文誌 Vol.2 No.1

        Al thin films were fabricated by using vacuum evaporation method and ion beam assisted deposition method. The effects of ion impact on Al thin films were characterized by the surface roughness and the SEM morphology. Al thin films fabricated by vacuum evaporation method were increasing surface roughness as increasing substrate temperature. Al thin films fabricated by ion beam assisted deposition method were decreasing hillock profiles and increasing density as increasing ion arrival ratio comparing to the Al thin films fabricated by vacuum evaporation method.

      • KCI등재후보

        벼 밭재배에 따른 수량 및 미질

        최원영,최민규,김상수,이규성,김태수,이재길 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.4

        벼 밭재배시 논벼 품종들의 적응성을 검토하기 위하여 마늘 간작으로 벼를 건답점파하여 재배한 결과를 요약하면 다음과 같다. 1. 입모율은 밭벼에 비해 논벼 품종이 낮았는데 그 순서는 상남밭벼〉동진찰벼〉신선찰벼〉진부찰벼 순이었다. 2. 출수기는 상남밭벼에 비해 진부찰벼는 3일 빨랐고 신선찰벼는 9일, 동진찰벼는 12일이 늦었다. 3. ㎡당 수수와 등숙비율은 밭벼에 비해 논벼품종들이 높았다. 4. 쌀수량은 상남밭벼에 비해 신선찰벼는 12%가 많았고 동진찰벼는 같았으며, 진부찰벼는 18%가 적었다. 5. 완전립비율은 진부찰벼에서 높았고 그 외는 비슷하였으며, 식미지수는 상남밭벼에 비해 동진찰벼는 높았으나 진주찰벼와 신선찰벼는 낮았다. To identify the rice varieties adaptable for upland cultivation, a series of experiments was carried out at the field(Chonnam series) of the Muan. Cheonnam province during 1999 - 2001. Four varieties, Jinbuchalbyeo(early-maturing variety), Sinseonchalbyeo (medium-maturing variety), Dongjinchalbyeo(medium-late maturing variety), and Sangnambatbyeo as a standard variety for upland were used in this experiment. Seedling establishment of the varieties was the highest in Sangnambatbyeo and followed by Dongjinchalbyeo under upland condition. Heading date in general delayed ranges from 9 to 12 days, while early-maturing variety, Jinbuchalbyeo was faster for 3 days compared with Sangnambatbyeo. Number of panicle per square meter was similar in both lowland and upland rice but percentage of ripened grain was high in lowland rice. Milled rice yield was high as of 12% in Sinseonchalbyeo compared with Sangnambatbyeo. Head rice ratio showed also the highest in Sinseonchalbyeo. The results indicate that Sinseonchalbyeo as glutinous rice variety could adapt well in this upland condition, and have good milling recovery as head rice as well as yield advantage.

      • High Density Plasma 발생 장치의 구성 및 특성

        황도원,손영호,김인수 경운대학교 산업기술연구소 1999 産業技術硏究論文誌 Vol.1 No.1(B)

        High density plasma deposition system was organized using electron cyclotron resonance source, and its plasma properties were measured by using the Langmuir probe method. The ECR microwave plasma conditions could be controlled by means of mass flow controller and ECR microwave power according to the vacuum pressure of 2.3×10^-5 Torr to 5.2×10^-2 Torr. The ion density was increased with an increase of gas flow rate, and a decrease of the distance between ECR source and substrates. The electron temperatures were decreased with an increase of gas flow rate, and a decrease of the distance between ECR source and substrates. The estimated plasma potential was from 27.0V to 31.5V

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