그람양성구균에 대한 Teicoplanin과 Vancomycin의 시험관내 항균력

최태열,김경숙,전용관,서일혜,김정욱,이웅수,안정열,김홍석,정재용,최효선,김덕언,유진우 대한감염학회 1994 감염 Vol.26 No.1

An increasing frequency of methicillin resistant S. aureus(MRSA), methicillin resistant coagulase negative staphylococci(MRCNS) and Enterococcal infection have been observed in recent years. Teicoplanin is a new glycopeptide antibiotic obstained from the Actinoplanes teicomycetius. The molecular structure and spectrum of antimicrobial activity of teicoplanin is simillar to those of vancomycin, and has been reported to have an excellent in vitro and in vivo effect against various gram-positive infections. Therefore, we evaluated the in vitor susceptibility of gram positive cocci, such as, S. aureus, coagulase negative Staphylococci(CNS), and Enterococci to teicoplanin and vancomycin. The total 253 strains consisted of MSSA(40), MRSA(53), MSCNS(47), MRCNS(48), and Enterococci(65). They were assayed by disc diffusion and agar dilution. During the study, 57% of S. aureus and 49% of CNS showed resistance to methicillin. The inhibitory diameter of teicoplanin was 15-20mm in MSSA, 12-19mm in MRSA, 13-24mm in MSCNS, 11-23mm in MRCNS, and 15-22mm in Enterococci respectively, and showed sensitivity in all but 8 strains(3.2%). The range of the minimum inhibitory concentration (MIC) of teicoplanin to MSSA, MRSA, MSCNS, MRCNS and Enterococci were 9.12-2.0㎍/ml, 0.25-2.0㎍/ml, & 0.25-32㎍/ml, 0.12-1.0㎍/ml respectively. One case of S. haemolyticus was resistant to teicoplanin (32㎍/ml) by the agar dilution method. Eight minor (3.2%) and one major(0.4%) error was observed when the MIC and disk diffusion data were correlated with teicoplanin. As for vancomycin the inhibitory diameter was 17-21mm in MSSA, 15-21mm in MRSA, 18-26mm in MSCNS, 18-25mm in MRCNS, and 16-22mm in Enterococci respectively. The range of the MIC of vancomycin to MSSA, MRSA, MSCNS, MRCNS, and Enterococci were 0.25-1.0㎍/ml, 0.25-4.0㎍/ml, 0.5-2.0㎍/ml and 0.5-2.0㎍/ml respectively. One minor error (0.4%) was seen with the vancomycin disk. The MIC90 of MSSA and MRSA exhibited the same results in teicoplanin (1.0㎍/ml, 1.0㎍/ml), and vancomycin(2.0㎍/ml, 2.0㎍/ml). MSCNS and MRCNS exhibited greater MIC90 with teicoplanin(4.0㎍/ml, 8.0㎍/ml) than vancomycin(2.0㎍/ml, 2.0㎍/ml). Incontrase Enterococci were more susceptible to teicoplanin(0.5㎍/ml) than to vancomucin (2.0㎍/ml). Results from this analysis indicated that both teicoplanin and vancomycin were very excellent for gram positive infections, especially those resistant to methicillin.

상아질 형성 부전증에 대한 증례보고

김희진,위유민,박성규,홍성수,조태식,이상호 조선대학교 구강생물학연구소 2002 口腔生物學硏究 Vol.26 No.2

Dentinogenesis imperfecta is an inherited disorder of dentin formation, usually exhibiting an autosomal dominant mode of transmission. Type Ⅰdentinogenesis imperfects occurs in Patients afflicted with osteogenesis imperfecta. Type Ⅱdentinogenesis imperfecta is not associated with osteogenesis imperfects. Type Ⅲ dentinogenesis imperfecta (Brandywine type) occurs in a racial isolate area in the state of Maryland. Clinically, the color of teeth may range from a gray to brownish violet or yellowish brown with translucent or opalescent hue. The enamel often chips and fractures away, allowing speedy attrition of the remaining, poorly calcified. softer dentin. Roentgenographic features include pulpal obliteration, bulbous crowns and short and blunted roots. The cementum, periodontal membrane and the supporting bone appear normal

소목(Caesalpinia sappan) 추출물의 Streptococcus mutans에 대한 성장, 산생성, 부착 및 비수용성 글루칸 합성 억제에 미치는 영향

유용욱,유현희,김윤정,유미선,서세정,이황,이흥수 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.2

Streptococcus mutans is known as the causative bacterial playing the most important role in forming plaque and it is being noticed as major causative bacteria of dental caries. Therefore, development of more effective, substantial and safe preventive agent against dental caries and periodontal disease is honestly required. The present study was designed to investigate the effect of chloroform extract(CE), n-butanol extract(BE),methanol extract(ME) and water extract(WE) of Caesalpinia on the growth, acid production, adhesion, and insoluble glucan synthesis of Streptococcus mutans. The CE, BE, ME and WE decreased the growth and acid production of S.mutans than that of control, especially, the ME was more effective than other extracts. The effect of CE, BE, ME and WE on the adherences to saliva-coated hydroxyapatite bead(S-HA) were evaluated. At the concentration of 2,000 ㎍/㎖, the CE showed 32% rate of bacterial adherence to S-HA, whereas, other extracts of the same concentration producedgreater than 50% rate. The CE and BE were significantly decreased the reduction rates of water insoluble glucan formation from sucrose by crude glucosyltransferase of S.mutans. Thus, the application of C. sappan can be considered a useful and a practical method for the prevention of dental caries.

좌심방 석회화와 관련된 반복된 좌심방 혈전 1예

김성훈,우성용,오유경,정우백,임철현,신재원,박철수,윤호중,정욱성,홍순조 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.3

퍼지제어를 이용한 루프검지식 교통 신호등의 최적주기연구

김원규,박종국,홍유식 慶熙大學校 1994 論文集 Vol.23 No.-

This is a study of the electro sensitive traffic system which uses sensors to both extend the cycle if there are many vehicles waiting at a stop light, and reduces it, if there are only a few. It was determined that each vehicle which passes through the loop detector neither is the same length, nor weighs the same, nor is going the same speed. Therefore, the conventional traffic control method is an inadequate measure of traffic volume and can cause delays and increaes waiting time. This paper proposes the optimal traffic cycle using fuzzy control as a system which will reduce vehicle waiting time and will improve the average speed a vehicle travels.

가상현실 기술을 활용한 중학교 과학 1 '지각의 물질' 단원의 수준별 WBI 개발

김희수,홍유정,이원국 공주대학교 과학교육연구소 2003 과학교육연구 Vol.34 No.-

우리는 7차 교육과정 중학교 과학 1'지각의 물질' 단원에 대한 가상현실 기술을 활용한 수준별 WBI를 개발하였다. 개발 결과는 인터넷에서 708명의 학습자들에게 투입되어 그 반응이 조사되었다. 학습 목표, 수업 설계, 화면의 구성, 이용의 편의성 등에 대한 응답결과는 80% 이상이 긍정적이었다. 따라서 본 프로그램은 사이버 학습에 활용할 수 있는 가치가 있다고 판단된다. We developed the WBI learning programs considered learner levels for the unit of the Earth crust materials of secondary school science 1 using virtual reality technique. The developed program has been evaluated with questionarie to 708 students after learning on the Web. The answer showed about 80% affirmatively. Therefore, this program will be able to use in cyber science 1 learning situation.

구조물의 비접촉 비파괴 검사를 위한 레이저 초음파법 적용

김재열,송경석,양동조,김유홍 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

The defects evaluation of the interior and the surface would be considered as vital characteristics in predicting the total life span of the steel structure. More importantly, the understandings in the interior composite of welding zone and the notifications in the presence, the formation, and the positioning of the non-metallic inclusion are necessary as well, since there were signs of relatively high defect frequency presented in the welding zone. The ultrasonic testing is a highly recommended technique chosen from among other techniques because of variety of advantages in conducting the non-destructive testing for the welding zone. However, the ultrasonic testing had technical disadvantages referred as followings; the problems due to the couplant between the PZT and the specimen, the formations that were miniature and complex, the moving subject, and the high temperature surrounding the specimen. This research was conducted to resolve the technical disadvantages of the contact ultrasonic testing by studying the non-contact ultrasonic testing where the ultrasonic waves were transferred by the laser, and revealing the specimen defects at its interior part and its surface part. The ultimate goal of this research was to develop a non-destructive evaluation applying the laser manipulated ultrasonic method for the steel structure

조기영어 교수법으로서의 전신반응법의 이론적 근거와 적용방법 탐색

홍성윤,김유미 中央大學校 韓國敎育問題硏究所 1995 韓國敎育問題硏究所論文集 Vol.- No.14

This study purports to investigate the theoretical basis of Total Physical Response(TPR) and its instructional applications. For this purpose of the study, firstly linguistic theories are presented, secondly. theoretical basis and characteristics of TPR are demonstrated, and thirdly, its applications and limitations in learning and teaching process are discussed, finally suggestions and conclusions on TPR are derived. This study also describes the developmental procedures for learning English through TPR in real situation using an example. TPR is defined as a language teaching method through associating language with physical activity. Motor activity is a right brain function that should precede left brain language processing. It complements the limitations of left brain based language teaching method. Based on criticisms of left-brain based language learning. TPR as an authentic, alternative language teaching method, has been welcomed to classroom setting. Many recent researches on TPR extol the many potential benefits of TPR for teaching and learning languages particularly compared to traditional left-brain based language teaching method. TPR invites students to comprehend a new language in the unit of a phrase or a sentence instead of a word. In addition, it promotes learning transfer. TPR matches the elementary school classroom instruction, because it teaches English through action according to the developmental level. TPR heavily utilizes the imperative mood, even into more advanced proficiency levels. The TPR classroom is one in which students do great deal of listening and activity before they speck, and their listening is accompanied by physical response. TPR puts emphases on right brain learning. TPR has its limitations. It seems to be especially effective in the beginning levels of language proficiency, but then it loses its distinctiveness as learners advance in their competence. In reading and writing activities students are limited to spinning off from the oral work in the classroom. All the students don't prefer learning language through action or physical activities. It is also difficult for teachers to incorporate TPR into the English Teaching program adequately in association with learning objectives. Teachers should not demand verbal response and should not guide too much until necessary.

초음파 비파괴 검사기법에 의한 용접결함 분류성능 비교

김재열,유신,김창현,송경석,양동조,김유홍 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

In this study, we made a comparative study of backpropagation neural network and probabilistic neural network and bayesian classifier and perceptron as shape recognition algorithm of welding flaws. For this purpose, variables are applied the same to four algorithms. Here, feature variable is composed of time domain signal itself and frequency domain signal itself. Through this process, we comfirmed advantages/disadvantages of four algorithms and identified application methods of four algorithms.

위암세포주에서 Recombinant Human Interferon-r와 Adriamycin의 투여순서가 항암효과에 미치는 영향

홍원선,손영숙,김창민,강윤구,이춘택,김유철,임영혁,남현석,이진오,강태웅 大韓免疫學會 1993 大韓免疫學會誌 Vol.15 No.-

Numerous previous studies, both in vitro and in vivo, have demonstrated that the cytotoxicity can be enhanced by the combination of chemotherapeutic agent and interferons(IFNs) in various types of cancer cells. We have previously reported that combined treatment of MKN-45, human gastric adenocarcinoma cells, with adriamycin(ADM) and recombinant human interferon-r(rh-IFN-r) increased in the cytotoxicity. In this study, the effects of combination timing of rh-IFN-r and ADM on the cytotoxicity against MKN-45 were investigated using MTT assay. MKN-45 was treated with rh-IFN-r and ADM in vitro on three schedules : Treat A ; rh-IFN-r and ADM were treated simultaneously, Treat B ; rh-IFN-r was treated 24 hours after the treatment with ADM, Treat C ; rh-IFN-r was treated for 72 hours and followed by the treatment with ADM. The survival of MKN -45 was inhibited by ADM dose-dependently. 102 and 103U/ml of rh-IFN-r significantly inhibited the survival of MKN-45(% survival : 35.1 ±-1.2% and 34.4 ±1.1% in Treat A and 42.5 ± 2.1% and 45.9-±2.5% in Treat C, respectively). However no difference in the survival was observed between 102 and 103U/ml of rh-IFN-r. Combined treatment with rh-IFN-r and ADM significantly augmented the cytotoxicity at low concentrations of ADM. Combined effects of rh-IFN-r and ADM were evaluated using IC30(,ag/ml) to ADM. IC30s of MKN-45 in Treat A, B and C at 102 U/ml of rh -IFN-r _ were 0.019 -?- 0.003, 0.045 :I:0.001 and 0.054 ± 0.012, respectively, while IC30 of MKN-45 treated with ADM alone was 0.052±0.004. IC30s of MKN-45 in ADM alone group, Treat A, Treat B and Treat C at 103U/ml of rh-IFN-r were 0.047 ±0.003, 0.004 -±0.001, 0.031 ±0.004 and 0.056 0.008, respectively. These results indicate IC30s of Treat A and B were significantly lower than those of ADM alone(p<0.05) and IC30s of Treat A was significantly lower than those of Treat B(p <0.01). IC30s of Treat C, however, were not different from those of ADM alone. From these results demonstrating that cytotoxic effects were increased by the combination of rh-IFN-r and ADM in the order, Treat A > Treat B> Treat C, it can be concluded that the simultaneous administration of rh-IFN-r and ADM may be the most effective method to combine these two therapeutic modalties.