http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Yong Luo,Ling Lan,Yong-Guang Jiang,Jia-Hui Zhao,Ming-Chuan Li,Neng-Bao Wei,Yun-Hua Lin 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.2
Although cancer stem cells (CSCs) play a crucial role in seeding the initiation of tumor progression, they do not always possess the same potent ability as tumor metastasis. Thus, precisely how migrating CSCs occur, still remains unclear. In the present study, we first comparatively analyzed a series of prostate CSCs, which exhibited a dynamically increasing and disseminating ability in nude mice. We observed that the transcriptional activity of HIF-1 and -catenin became gradually elevated in these stem cells and their epithelial-mesenchymal transition (EMT) characteristic altered from an epithelial type to a mesenchymal type. Next, we further used cancer-associated fibroblasts (CAFs), which were cultured from surgically re-sected tissues of prostate cancer (PCa) to stimulate prostate CSCs. Similar results were reconfirmed and showed that the protein levels of both HIF-1 and -catenin were markedly improved. In addition, the EMT phenotype displayed a homogenous mesenchymal type, accompanied with increased aggressive potency in vitro. Most importantly, the aforementioned promoting effect of CAFs on prostate CSCs was completely repressed after “silencing” the activity of -catenin by transfection of stem cells with ShRNA. Taken together, our observations suggest that prostate migrating CSCs, with a mesenchymal phenotype, could be triggered by CAFs in a HIF-1alpha/beta-catenin-depen-dent signaling pathway.
( Luo Ming Fang ),( Xing Jian Min ),( Gou Zhong Xuan ),( Liu Hui Zhou ),( Chen Jia Yong ) 한국화학공학회 2003 Korean Journal of Chemical Engineering Vol.20 No.4
The desulfurization of dibenzothiophene (DBT), 4,6-dimethyldibenzothiophene (4,6-DMDBT) and their mixture by lyophilized cells of Pseudomonas delafieldii R-8 was studied in the presence of dodecane. The desulfurization rate for 4,6-DMDBT was found to be about 40% in comparison with that for DBT. The desulfurization process for DBT and 4,6-DMDBT proceeded simultaneously without preference for either one. The desulfurization rate for each compound was decreased when they were mixed together. The extent of desulfurization of 4,6-DMDBT was increased with the increase of cell concentration and the decrease of the volume ratio of oil-to-water used. The specific desulfurization rate for 4,6-DMDBT could be reached to 10.4 mmol sulfur kg^(-1) (cell) h^(-1) [approximately 0.33 mg sulfur g^(-1) (cell) h^(-1)]. Pseudomonas delafieldii R-8 showed high desulfurization capability for straight-run diesel oil (containing 1,807 mg/L of sulfur). About 1,000 mg/L of sulfur in diesel oil was removed by resting cells of this strai in 24 h of reaction. The specific desulfurization rate was 8.75 mmol sulfur kg^(-1) (cell) h^(-1).
( Na Luo ),( Guan Li ),( Yong Qing Li ),( Xiong Wei Fan ),( Yue Qun Wang ),( Xiang Li Ye ),( Xiao Yan Mo ),( Jun Mei Zhou ),( Wu Zhou Yuan ),( Ming Tan ),( Hua Ping Xie ),( Karen Ocorr ),( Rolf Bodmer 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.5
The sterile alpha motif (SAM) is a putative protein interaction domain involved in a wide variety of biological processes. Here we report the identification and characterization of a novel gene, SAMD4B, which encodes a putative protein of 694 amino acids with a SAM domain. Northern blot and RT-PCR analysis showed that SAMD4B is widely expressed in human embryonic and adult tissues. Transcriptional activity assays show SAMD4B suppresses transcriptional activity of L8G5-luciferase. Over-expression of SAMD4B in mammalian cells inhibited the transcriptional activities of activator protein-1 (AP-1), p53 and p21, and the inhibitory effects can be relieved by siRNA. Deletion analysis indicates that the SAM domain is the main region for transcriptional suppression. The results suggest that SAMD4B is a widely expressed gene involved in AP-1-, p53-and p21-mediated transcriptional signaling activity. [BMB reports 2010; 43(5): 355-361]
( Yi Yong Luo ),( Jin Kui Yang ),( Ming Liang Zhu ),( Jin Ping Yan ),( Minghe Mo ),( Ke Qin Zhang ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.1
Four series (S, M, R, and W) of Alternaria longipes isolates were obtained based on consecutive selection with Dimethachlon (Dim) and ultraviolet irradiation. These isolates were then characterized according to their tolerance to Dim, sensitivity to osmotic stress, and phenotypic properties. All the selected Dim-resistant isolates showed a higher osmosensitivity than the parental strains, and the last generation was more resistant than the first generation in the M, R, and W series. In addition, the changes in the Dim resistance and osmotic sensitivity were not found to be directly correlated, and no distinct morphologic characteristics were found among the resistant and sensitive isolates, with the exception of the resistant isolate K-11. Thus, to investigate the molecular basis of the fungicide resistance, a group III two-component histidine kinase (HK) gene, AIHK1, was cloned from nineteen A. longipes isolates. AIHK1p was found to be comprised of a six 92-amino-acid repeat domain (AARD), HK domain, and response regulator domain, similar to the Os-1p from Neurospora crassa. A comparison of the nucleotide sequences of the AIHK1 gene from the Dim-sensitive and -resistant isolates revealed that all the resistant isolates contained a single-point mutation in the AARD of AIHK1p, with the exception of isolate K-11, where the AIHK1p contained a deletion of 107 amino acids. Moreover, the AIHK1p mutations in the isolates of each respective series involved the same amino acid substitution at the same site, although the resistance levels differed significantly in each series. Therefore, these findings suggested that a mutation in the AARD of AIHK1p was not the sole factor responsible for A. longipes resistance to dicarboximide fungicides.
UF/UV/O<sub>3</sub>/촉매 공정에 의한 축산폐수의 처리특성
나명한 ( Ming-han Luo ),이길용 ( Gil-yong Lee ),정태섭 ( Tae-seop Jeong ) 한국환경기술학회 2010 한국환경기술학회지 Vol.11 No.2
본 연구는 축산폐수처리를 위한 고급산화공정의 적용가능성과 처리효율 및 범위를 실험하여 축산폐수처리에 대한 기초 자료로 제시하고자 한다. 실행시에 폐수 속 부유물질의 존재는 자외선의 광원이 촉매에 효율적으로 조사에 영향을 주기 때문에 SS의 제거과정을 우선 행하였다. UF막의 효과적인 이용으로 SS는 거의 제거되었으며, 그다음 회분식 반응기에 고급산화공정으로 pH와 순환유량을 변화하면서 실험을 행한 결과 산성영역 특히 pH에서 처리 효과가 우수하게 나타났으며, 순환유량이 대체적으로 증가 할수록 제거효율이 높아지는 경향을 보였다. This study was performed to provide basic information for evaluating the efficiency and applicable extent of photo-catalysis for the treatment of swine wastewater. Presence of suspended solids in the wastewater may affect effectively irradiation of ultraviolet light and removal process of the dissolved organics At first, SS was almost removed through effective use of UF membrane. Resultly, application of advanced oxidation process in the batch reactor showed that removal efficiency was more excellent in acidic pH and increased with the circular flow.
Fang, Lu,Xu, Xian-Yong,Luo, An,Li, Yan,Tu, Chun-Ming,Fang, Hou-Hui The Korean Institute of Power Electronics 2012 JOURNAL OF POWER ELECTRONICS Vol.12 No.6
A new kind of resonant impedance type hybrid active filter (RITHAF) is proposed for dynamic harmonic current suppression and high capacity reactive compensation in medium and high voltage systems. This paper analyzed the different performance of the RITHAF when the active part of the RITHAF is controlled as a current source and as a voltage source, respectively. The harmonic suppression function is defined in this paper. The influences of the changes caused by the grid impedance and the detuning of the passive power filter on the compensating characteristics of the RITHAF are studied by analyzing the suppression function. Simulation and industrial application results show that the RITHAF has excellent performances in harmonic suppression and reactive compensation, which is suitable for medium and high voltage systems.
Lu Fang,Xian-yong Xu,An Luo,Yan Li,Chun-ming Tu,Hou-hui Fang 전력전자학회 2012 JOURNAL OF POWER ELECTRONICS Vol.12 No.6
A new kind of resonant impedance type hybrid active filter (RITHAF) is proposed for dynamic harmonic current suppression and high capacity reactive compensation in medium and high voltage systems. This paper analyzed the different performance of the RITHAF when the active part of the RITHAF is controlled as a current source and as a voltage source, respectively. The harmonic suppression function is defined in this paper. The influences of the changes caused by the grid impedance and the detuning of the passive power filter on the compensating characteristics of the RITHAF are studied by analyzing the suppression function. Simulation and industrial application results show that the RITHAF has excellent performances in harmonic suppression and reactive compensation, which is suitable for medium and high voltage systems.
Jiangbo He,Hua-Jie Zhu,Gui-Fen Luo,Guang-Ming Liu,Yan Li,Hao Chen,Shaopeng Chen,Xin Lu,Guochun Zhou,Yong-Xian Cheng 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.8
The whole plant of Euphorbia helioscopia is an important traditional Chinese medicine. Fom its BuOH soluble extract,one new lactam (1), three new terpenoids (2-4) including a new naturally occurring compound, and three known compounds were isolated. Their structures were identified by spectroscopic evidences. In particular, the absolute configurations of side chain of compounds 1 and 2 were determined using computational methods.
Intermedins A and B; New Metabolites from Schisandra propinqua var. intermedia
Hong-Mei Li,Chun Lei,Yong-Ming Luo,Xiao-Nian Li,Xiao-Lei Li,Jian-Xin Pu,San-Yun Zhou,Rong-Tao Li,Han-Dong Sun 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6
A new dibenzocyclooctadiene lignan, intermedin A (1), and a new natural bisabolane sesquiterpenoid, intermedin B (2), were isolated from the aerial parts of Schisandra propinqua var. intermedia. Their structures were elucidated on the basis of extensive spectroscopical analysis.