Bioinformatic prediction and analysis of glucolipid metabolic regulation by miR-34a in Megalobrama amblycephala

Ling‑Hong Miao,Wen‑Jing Pan,Yan Lin,Bo Liu,Ming‑Chun Ren,Qun‑Lan Zhou,Xian‑Ping Ge 한국유전학회 2017 Genes & Genomics Vol.39 No.12

The objective of this study was to analyze the target genes and regulatory function of miR-34a in Megalobrama amblycephala using second-generation highthroughput sequencing and bioinformatic tools. Functional enrichment analysis was performed by gene ontology. MiR- 34a and target gene expression levels were measured in M. amblycephala fed normal and high-carbohydrate diets. The results revealed that miR-34a was highly conserved in several species, and miR-34a of M. amblycephala has a close evolutionary relationship to that of zebrafish and common carp. miRanda, TargetScan, RNAhybrid predicted 5,185, 6,282 and 2,168 target genes, respectively, and 645 target genes were in common. According to annotation information, the target genes were enriched in phosphate metabolism, glycerophospholipid metabolism, Golgi vesicle transport, cell division, and other biological processes (P < 0.05). Pathway enrichment analysis revealed that these target genes were mainly enriched in alpha-linolenic acid and linoleic acid metabolism, ether lipid metabolism, VEGF signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, and MAPK signaling pathway (P < 0.05). The regulatory role of miR-34a was more significant in the liver than in the brain of M. amblycephala. MiR-34a regulates glucose lipid homeostasis induced by high glucose diets by upregulating hepatic PI3K/Akt, FOXO, and TOR signaling pathways.

Efficacy of the predator Mallada basalis (Neuroptera: Chrysopidae) on Tetranychus kanzawai and Panonychus citri (Acari: Tetranychidae) at different predator:prey release ratios

Ling Lan Cheng,James R. Nechols,David C. Margolies,James F. Campbell,Ping Shih Yang,Chien Chung Chen,Chiu Tung Lu 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.1

Wecompared population suppression of the phytophagous mites, Tetranychus kanzawai Kishida and Panonychus citri (McGregor), on papaya by second instar larvae of the green lacewing, Mallada basalis (Walker), at various predator:prey release ratios in the laboratory. Initially, we presented M. basalis with mixed age classes of each mite species separately at a density of approximately 30 mites per seedling. After 3 days, predator:prey ratios of 1:30, 1:15, and 1:10 resulted in reductions of T. kanzawai of 66.8%, 82.6%, and 83.3%, respectively, and reductions of P. citri of 41.8%, 75.5%, and 77.2%, respectively. Predation on individual age classes was approximately equal in both species, reinforcing previous findings that this predator does not show a preference among age classes. We next presented M. basalis with mixed populations of the two mite species in which there were equal numbers of each species and the density was as in the single species tests. Total mite reduction with both mite species present was 48.5%, 71.9%, and 74.5% at ratios of 1:30, 1:15, and 1:10, respectively; T. kanzawai was reduced by 50.5%, 77.4%, and 79.5%, respectively, and P. citriwas reduced by 44.1%, 60.3%, and 63.2%, respectively. This study suggests that M. basalis has the potential for substantially suppressing populations of both T. kanzawai and P. citri on papaya at a predator:prey ratio of 1:15 or greater. However, evaluation under realistic agricultural settings is needed before specific recommendations about predator release rates can be made.

Multicolour carbon dots with excitation‑independent emission by microwave solvothermal reaction

Xiao‑Lan Wei,Qin‑Ling Shi,Lan Jiang,Yu Qin 한국탄소학회 2023 Carbon Letters Vol.33 No.1

Carbon dots (CDs) with tunable fluorescence emissions have been developed from a wide range of small organic molecules with various bottom-up syntheses. However, most of them were prepared under high temperatures and high pressures with long reaction times and tedious purification processes. In addition, previously reported carbon dots frequently displayed excitation-dependent emissions, which restrict their further applications. Herein, we present a simple and rapid microwaveassisted solvothermal synthesis of multicolour carbon dots with excitation-independent emissions. In ethylene glycol, the green (G)-CDs emitting at 537 nm with a quantum efficiency (QY) of 15% were obtained by using a single precursor of phloroglucinol, and blue (B)- and yellow (Y)-CDs emitting at 436 nm and 557 nm with QYs of 55% and 28% were derived with additives of o- and m-phenylenediamine, respectively. Analyses of their chemical structures and optical processes suggest that highly polymeric carbon dots were uniformly formed from the small molecules and their fluorescences were predominantly originated from rapid direct recombination. Furthermore, emissions at different wavelengths were mainly attributed to different degrees of oxidation (13.9%, 15.2% and 16.4% oxygen in B-, G- and Y-CDs, respectively) and different proportions of pyrrolic nitrogen (10.4% and 1.40% in B- and Y-CDs, respectively). To demonstrate the application feasibility, the obtained carbon dots were utilized for ion detection and anti-counterfeiting. Based on static quenching of the carbon dots’ fluorescence, micro amounts of ferric ion in water samples were detected selectively and reproducibly. Moreover, the anti-counterfeiting pattern constructed by the carbon dots emitted fluorescence under ultraviolet illumination, but concealed perfectly under daylight. This achievement is of great potential for developing multicolour carbon dots of high qualities.

Research about the Evaluation Index of Agricultural Sightseeing Garden Landscape Resources based on AHP

( Yu Lan Ling ),( Piao Yong Ji ),( Jiang Dao Zhu ),( Jo Hyunju ) 한국환경과학회 2016 한국환경과학회지 Vol.25 No.4

On the basis of conducting a comprehensive investigation to agricultural sightseeing garden landscape resources, we established a evaluation index system of agricultural sightseeing garden landscape resources. From the perspective of experts, comparing the importance of all levels indicators, we obtained the weights of landscape resources evaluation index through using the Analytic Hierarchy Process. The result showed that the descending order of the weights of influence of agricultural sightseeing garden nineteen evaluation index is: reach ability (0.128), safety (0.083), location (0.078), participatory (0.076), cultural value (0.058), ecological conditions (0.057), scenic beauty (0.0505), environmental quality (0.051), featured properties (0.0501), environmental tolerance (0.048), reputation (0.047), environmental capacity (0.045), humanize (0.041), spots configuration (0.034), applicable travel period (0.033), scientific value (0.032), art value (0.031), holistic (0.03), suitability (0.027), it can provide a framework and basis for the planning, management, protection and exploitation of agricultural sightseeing garden landscape resources.

Research on the Video Advertising Detection Based on LS-SVM

Xiao-ling Lan,Shutuan Zhang 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.7

Aimed to the difficult problem of detection information of video advertising from the massive video data, the novelty detection method of the video advertising based on the least squares support vector machine (LS-SVM) algorithm is introduced after analyzing and studying the feature of video advertising. The detection of the abrupt change shot and the gradual change shot in video data is realized by using color histogram algorithm, and the classification of the shot is achieved by using the particle swarm optimization and least squares support vector machine algorithm. The experiments show that the method based on the color histogram algorithm and LS-SVM algorithm has better Precision and recall, and can realize the accuracy detection of the video advertising.

Preliminary Study of the Liouduei Area Hakka Industry and Cultural Tourism Using Analytic Hierarchy Process Method

Chuang Lan-Ying,Liu Hui-Ling,Chen Jui-Ling,Kuo Shiunn-Der 세계문화관광학회 2011 Conference Proceedings Vol.12 No.-

Taiwan is an island of diverse ethnicities and each ethnic group has maintained its unique traditional industry and culture. The saying "Tung Flower in the North and Liouduei in the South" is commonly used to describe the Hakka communities in Taiwan. "Liouduei" is not an administrative district that could be found on the map. The term refers to the Kaohsiung and Pingtung Hakka settlement in Southern Taiwan. In recent years the Council for Hakka Affairs of the Executive Yuan and the local government has made significant efforts to cultivate and support local culture and industry development. They actively marketed the Hakka cultural industry internationally and connected the local Hakka industry with cultural tourism hoping to raise public awareness of Hakka culture and establish a sustainable Hakka industry in a long run. In this study key evaluation criteria and literature were collected and reviewed. And there were 5 evaluation component fields 16 evaluation criterion items drawn-up. The Analytic Hierarchy Process (AHP) method was applied to conduct the criteria evaluation analysis of the Liouduei area Hakka Industry and cultural tourism with the target groups including the municipal Hakka administrative agencies social organizations Hakka Industry Business and experts and scholars. The findings were summarized into a cohesive conclusion and the results of the analysis were provided for government agencies as future references for their work on the preservation of Hakka cultural tourism and promotion of Hakka industry. Overall the study suggested that the interviewees ranked "Product Quality Assurance"(0.303) as the most critical factor and ranked the "The Maintenance of Industry and Cultural "(0.299) as the second factor and "Government Subsidies and Promotion"(0.232) as the third factor and followed by "Economic Efficiency Improvement"(0.085) ranked as the fourth factor. Finally it ranked the "Hakka Industry Business Alliance" (0.081) as the fifth factor.

Recent progresses and perspectives of VN-based materials in the application of electrochemical energy storage

Ling-Na Shi,Xue-Zhong Li,Lan-Tong Cui,Peng-Fei Wang,Ying Xie,Ting-Feng Yi 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

Developing well-behaved electrode materials is crucial for manufacturing high-performance batteries,SCs and electrocatalysis, and it is also one of the main driving forces for the development of EES devices. In consideration of the high robustness and electrical conductivity during the electrochemical reactionprocess, VN-based materials exhibit good performance as electrode materials (or catalytic materials)for batteries, supercapacitors, and excellent catalytic activity for electrocatalysts. Especially, the VNbasedmaterials decorated into other active compounds with various morphologies elaborately presentexcellent performances due to the abundant active sites and fully synergistic effect. This review presentsthe structure-performance relation by designing different-dimensional nanostructures and various applicationsof VN-based materials in electrochemical energy storage (EES) applications. Finally, the perspectiveson future challenges and progress have been discussed. This review can offer a specificunderstanding for the optimization strategies of VN-based materials, thus booming the rapid developmentand practical applications for EES devices in the future.

A Fermentation Strategy for Anti-MUC1 C595 Diabody Expression in Recombinant Escherichia Coli

Lan, John Chi-Wei,Ling, Tau Chuan,Hamilton, Grant,Lyddiatt, Andrew The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.5

The development of fermentation conditions for the production of C595 diabody fragment (dbFv) in E. coli HB2151 clone has been explored. Investigations were carried out to study the effect of carbon supplements over the expression period, the comparison of C595 dbfv production in synthetic and complex media, the influence of acetic acid upon antibody production, and comparison of one-stage and two-stage processes operated at batch or fed-batch modes in bioreactor. Yeast extract supplied during expression yielded more antibody fragment than any other carbon supply. The synthetic medium presented higher specific productivity (0.066 mg dbFv $g^{-1}$ dry cell weight) when compared to the complex medium (0.044 mg dbFv $g^{-1}$ DCW). The comparison of fermentation strategies demonstrated that (1) one-stage fed-batch fermentation performed higher C595 dbFv production than that operated in batch mode which was significantly affected by acetate concentration; (2) a two-stage batch operation could enhance C595 dbFv production. It was found that a concentration of 12.3 mg $L^{-1}$ broth of C595 dbFv and a cell concentration of 10.8g $L^{-1}$ broth were achieved at the end of two-stage operation in 5-L fermentation.

Genomic Sequence Analysis and Organization of BmKαTx11 and BmKαTx15 from Buthus martensii Karsch: Molecular Evolution of α-toxin genes

Xiu Ling Xu,Zhi Jian Cao,Ji Qun Sheng,Wen Lan Wu,Feng Luo,Yong Gang Sha,Xin Mao,Hui Liu,Da He Jiang,Wen Xin Li 한국생활환경학회 2005 BMB Reports Vol.38 No.4

Synthesis, Structures and Photoluminescent Properties of Two Novel Zinc(II) Compounds Constructed from 5-Sulfoisophthalic Acid

Yu-Lan Zhu,Xue-Ling Tang,Kui-Rong Ma,Hao Chen,Feng Ma,Lian-Hua Zhao 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.7

Hydrothermal reaction of zinc(II) salts with 5-sulfoisophthalic acid monosodium salt (NaO3SC6H3-1,3-(COOH)2, NaH2-SIP) and 1,10-phenanthroline (phen) led to two new compounds, [Zn(phen)3]·2H2SIP·4H2O (1) and [Zn(phen)2(H2O)2]·2H2SIP·2H2O (2). They were characterized by element analysis, IR spectroscopy, thermalgravimetric analysis (TGA),X-ray powder diffraction (XRD), and single-crystal X-ray diffraction. Both compounds 1-2 represent the first example of Zn/phen/SIP system. The Zn (II) ion in 1 is six-coordinated by six nitrogen atoms from three phen molecules, and the H2SIP‒ ligands engage in the formation of hydrogen bond. The Zn(II) ion in 2 is coordinated by four nitrogen atoms from two phen molecules and two oxygen atoms from two water molecules. Moreover, both 1 and 2 are assembled into 3D supramolecular architectures by hydrogen bonds (O-H···O) and π-π interactions. Solvent water molecules occupying voids of the compounds serve as receptors or donors of the extensive O-H···O hydrogen bonds.