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Ren, Yong-Qiang,Han, Ju-Qiang,Cao, Jian-Biao,Li, Shao-Xiang,Fan, Gong-Ren Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11
Objective: The objective of this study was to evaluate the association of MDR1 gene polymorphisms with susceptibility to hepatocellular carcinoma (HCC). Methods: A total of 689 HCC patients and 680 cancer-free subjects were enrolled. Human MDR1 gene polymorphisms were investigated by created restriction site-polymerase chain reaction (CRS-PCR) and DNA sequencing methods. Multiple logistic regression models were applied to estimate the association between MDR1 gene polymorphisms and susceptibility to HCC. Results: We detected a novel c.4125A>C polymorphism and our findings suggested that this variant was significantly associated with susceptibility to HCC. A significantly increased susceptibility to HCC was noted in the homozygote comparison (CC versus AA: OR=1.621, 95% CI 1.143-2.300, ${\chi}^2$=7.4095, P=0.0065), recessive model (CC versus AC+AA: OR=1.625, 95% CI 1.167-2.264, ${\chi}^2$=8.3544, P=0.0039) and allele contrast (C versus A: OR=1.185, 95% CI 1.011-1.389, ${\chi}^2$=4.4046, P=0.0358). However, no significant increase was observed in the heterozygote comparison (AC versus AA: OR=0.995, 95% CI 0.794-1.248, ${\chi}^2$=0.0017, P=0.9672) and dominant model (CC+AC versus AA: OR=1.106, 95% CI 0.894-1.369, ${\chi}^2$=0.8560, P=0.3549). Conclusions: These findings suggest that the c.4125A>C polymorphism of the MDR1 gene might contribute to susceptibility to HCC in the Chinese population. Further work will be necessary to clarify the relationship between the c.4125A>C polymorphism and susceptibility to HCC on larger populations of diverse ethnicity.
Yong-Qiang Zhao,Guo-Di Liu,Cong-Cong Hou,Ying-Li Han,Jun-Quan Zhu,Jun-Quan Zhu 대한독성 유전단백체 학회 2016 Molecular & cellular toxicology Vol.12 No.3
Catalase (CAT) is an essential antioxidant in organisms, its can eliminate hydrogen peroxides produced in the cellular environment. Previous studies have focused mainly on the functions of CAT by pathogen challenge, but the functions of CAT by metals stress remain unclear. In order to investigate the function of CAT in Acrossocheilus fasciatus (AfCat), we cloned CAT complete cDNA sequences and analyzed its functions by cadmium (Cd) stress. qPCR analysis illustrated that the expression of CAT changed rapidly and dynamically in response to Cd from 0.05 mg L-1 to 1.25 mg L-1. Enzyme activity assay result showed that CAT activity was up-regulated significantly in response to Cd from 0.05 mg L-1 to 1.25 mg L-1. Histochemistry analysis showed that the structure of liver, testis and kidney were severely damaged by exposed to Cd of 1.25 mg L-1. Collectively, the results of our study indicate that AfCat may play an indispensable role in maintaining redox balance under Cd exposure, but AfCat expression will be inhibited when Cd accumulation reaches to the critical concentration. This study will illuminate the potential functions of AfCat in the defense mechanisms of reproductive and metabolic systems under Cd exposure in A. fasciatus.
Zhao-Yong Zhu,Qing-Ming Shi,Bao-Feng Han,Xian-Feng Wang,Sheng Qiang,Chun-Long Yang 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.9
Twenty novel tetramic acid derivatives (E)-3-(1-(alkyloxyamino)ethylidene)-1-alkylpyrrolidine-2,4-diones were synthesized by the reaction of 3-(1-hydroxyethylidene)pyrrolidine-2,4-diones with O-alkyl hydroxylamines. The title compounds were confirmed by IR, 1H NMR, MS and elemental analysis. The structure of compound 6r was further verified by X-ray diffraction crystallography. The bioassays showed that most of the title compounds exhibited noticeable herbicidal and fungicidal activities.
A novel panel of serum miR-21/miR-155/miR-365 as a potential diagnostic biomarker for breast cancer
Ji-Guang Han,Yong-Dong Jiang,Chun-Hui Zhang,Yan-Mei Yang,Da Pang,Yan-Ni Song,Guo-Qiang Zhang 대한외과학회 2017 Annals of Surgical Treatment and Research(ASRT) Vol.92 No.2
Purpose: Insufficient sensitivity and specificity prevent the use of most existing biomarkers for early detection of breast cancer. Recently, it was reported that serum microRNAs (miRNAs) may be potential biomarkers in many cancer diseases. In this study, we investigated whether serum levels of 5 miRNAs including miR-21, miR-125b, miR-145, miR-155, and miR-365 could discriminate breast cancer patients and healthy controls. Methods: Serum levels of miRNAs were measured by using quantitative real-time polymerase chain reaction in 99 breast cancer patients and 21 healthy controls. The abundance change of serum miRNAs were also evaluated following surgical resection in 20 breast cancer patients. Receiver operating characteristic (ROC) curve analysis was performed to assess the sensitivity and specificity of miRNAs as diagnostic biomarkers. Results: Serum levels of miR-21 and miR-155 was significantly higher, while miR-365 was significantly lower in breast cancer as compared with healthy controls. The serum levels of miR-21 and miR-155 significantly decreased following surgical resection. Additionally, the serum level of miR-155 at stages I and II was significantly higher compared to stage III. The serum miR-145 level was remarkably higher in progesterone receptor (PR)-positive patients than PR-negative. The positivity of miR-21, miR-155, and miR-365 was high compared to CA 153 and CEA in breast cancer. ROC curve analyses of a combination of miR-21, miR-155, and miR-365 yielded much higher area under curve and enhanced sensitivity and specificity in comparison to each miRNA alone. Conclusion: The combination of serum miR-21/miR-155/miR-365 may potentially serve as a sensitive and specific biomarker that enables differentiation of breast cancer from healthy controls.
Zhu, Zhao-Yong,Shi, Qing-Ming,Han, Bao-Feng,Wang, Xian-Feng,Qiang, Sheng,Yang, Chun-Long Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.9
Twenty novel tetramic acid derivatives (E)-3-(1-(alkyloxyamino)ethylidene)-1-alkylpyrrolidine-2,4-diones were synthesized by the reaction of 3-(1-hydroxyethylidene)pyrrolidine-2,4-diones with O-alkyl hydroxylamines. The title compounds were confirmed by IR, $^1H$ NMR, MS and elemental analysis. The structure of compound 6r was further verified by X-ray diffraction crystallography. The bioassays showed that most of the title compounds exhibited noticeable herbicidal and fungicidal activities.
Gwak, Yunho,Hwang, Yong-sic,Wang, Baobei,Kim, Minju,Jeong, Jooyeon,Lee, Choul-Gyun,Hu, Qiang,Han, Danxiang,Jin, EonSeon Oxford University Press 2014 Journal of experimental botany Vol.65 No.15
<P><I>Haematococcus pluvialis</I> cells predominantly remain in the macrozooid stage under favourable environmental conditions but are rapidly differentiated into haematocysts upon exposure to various environmental stresses. Haematocysts are characterized by massive accumulations of astaxanthin sequestered in cytosolic oil globules. Lipidomic analyses revealed that synthesis of the storage lipid triacylglycerol (TAG) was substantially stimulated under high irradiance. Simultaneously, remodelling of membrane glycerolipids occurred as a result of dramatic reductions in chloroplast membrane glycolipids but remained unchanged or declined slightly in extraplastidic membrane glycerolipids. <I>De novo</I> assembly of transcriptomes revealed the genomic and metabolic features of this unsequenced microalga. Comparative transcriptomic analysis showed that so-called resting cells (haematocysts) may be more active than fast-growing vegetative cells (macrozooids) regarding metabolic pathways and functions. Comparative transcriptomic analyses of astaxanthin biosynthesis suggested that the non-mevalonate pathway mediated the synthesis of isopentenyl diphosphate, as the majority of genes involved in subsequent astaxanthin biosynthesis were substantially up-regulated under high irradiance, with the genes encoding phytoene synthase, phytoene desaturase, and β-carotene hydroxylase identified as the most prominent regulatory components. Accumulation of TAG under high irradiance was attributed to moderate up-regulation of <I>de novo</I> fatty acid biosynthesis at the gene level as well as to moderate elevation of the TAG assembly pathways. Additionally, inferred from transcriptomic differentiation, an increase in reactive oxygen species (ROS) scavenging activity, a decrease in ROS production, and the relaxation of over-reduction of the photosynthetic electron transport chain will work together to protect against photooxidative stress in <I>H. pluvialis</I> under high irradiance.</P>
Zhong Chang Liu,Xing Yuan,Jing Tian,Yong Qiang Han,Kai Bo Yu,Peng Kun Teng 한국자동차공학회 2018 International journal of automotive technology Vol.19 No.5
The object of this paper is to reduce soot emissions under typical 5s transient conditions of constant speed and increasing torque. And effects of fuel injection timing on combustion and emissions parameters were experimentally and numerically studied in a regulated two-stage turbocharged diesel engine with a turbine bypass valve (TBV). The test results indicated that: the smaller TBV opening could improve deterioration of smoke emissions and BSFC at medium and heavy loads. Afterward, the full-stage injection timing (FSIT) strategies (delaying injection timing during the entire transient process) could reduce soot and NOX emissions simultaneously. However, when TBV opening became larger, smoke emissions and BSFC were deteriorated gradually. Moreover, the sectional-stage injection timing (SSIT) strategies (advancing injection timing from 10 % load to a preset load and delaying injection timing from the preset load to 100 % load) could markedly reduce soot emissions by 75.8 % with TBV opening 20 %; the degradation of fuel consumption could be effectively suppressed. Finally, coupling the SSIT strategies with the TBV control strategies could significantly improve the transient performance.
Chemical Constituents from the Aerial Parts of Isodon coetsa and their Cytotoxicity
Wei Zhao,Jian Xin Pu,Xue Du,Yong Zhao,Fei He,Hai Bo Zhang,Yong Bo Xue,Wei Lie Xiao,Han Dong Sun,Ying Li Wu,Guo Qiang Chen 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.12
Three new compounds (1-3), including a neolignan, a triterpenoid, and a diterpenoid, together with twenty known compounds (4-23), were isolated from the aerial parts of Isodon coetsa. Their structures and relative configurations were elucidated on the basis of spectroscopic data. Compounds 1, 3, 5-9, 11-13, 16-17, and 19-23 were evaluated for their cytotoxicity against HT-29, BEL-7402, and SK-OV-3 human tumor cell lines. Compound 7 showed significant inhibitory effects on all three types of cells, with IC50 values of 2.52, 3.06, 2.14 μM, respectively.
Mechanism of heterogeneous mercury oxidation by HCl on V2O5(001) surface
Li Zhao,Yu Liu,Yang-wen Wu,Jian Han,Song-lin Zhang,Qiang Lu,Yong-ping Yang 한국물리학회 2018 Current Applied Physics Vol.18 No.6
The selective catalytic reduction (SCR) system for NOX removal in coal-fired power plants has a promoting effect on the oxidation and removal of elemental mercury. In this study, basic mechanism of mercury oxidation by V2O5-based SCR catalyst is investigated via density functional theory method and the periodic slab models. Calculations are conducted to determine the adsorption energies and geometries of Hg0, HgCl, HgCl2 and HCl on V2O5(001) surface, and to reveal the energy profile of oxidation reaction and the structures of relative transition states and intermediates. The results indicate that HCl can significantly promote Hg0 oxidation on V2O5(001) surface, by forming an intermediate HgCl-surface which is important for Hg0 oxidation. The Hg0 oxidation goes through Hg0→HgCl→HgCl2, and the two stages of the reaction follow Eley–Rideal mechanism and Langmuir- Hinshelwood mechanism, respectively. The formation of HgCl2 is the rate-determining step due to its high energy barrier. Three detailed reaction pathways are obtained, and the related energy profiles and structures are analyzed in detail. The Hg0 oxidation reaction can take place through all three pathways even if differences exist in each other, while pathways I and II have relatively low energy barriers.