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Ye Seul Park(박예슬),Gun He Nam(남건희),Kyung Jo Jo(조경조),Hye Won Kawk(곽혜원),Je-Geun Yoo(유제근),Jin Dong Jang(장진동),Sang Moon Kang(강상문),Sang Yong Kim(김상용),Young Min Kim(김영민) 한국생물공학회 2019 KSBB Journal Vol.34 No.3
Collagen is decomposed by MMP-1, an enzyme induced by transcription factor activator protein 1 (AP-1) and matrix metalloproteinase-9 (MMP-9). Action of MMPs in inflammatory response promotes inflammatory cell movement and secretion, resulting in wrinkles on the skin. After using Bacillus sp. fermentation system and water, Artemisia vulgaris was fermented to prepare different solvent fractions using water, dichloromethane, hex ane, n-butanol, and ethyl acetate. These fractions were used to assess their effects on cell survival, wound healing, MMP-1/MMP-9 and procollagen type I C-peptide (PICP) expression, and skin turnover. MTT assay showed that cell viability of each treated group was 103% to 121%, indicating that A. vulgaris fractions were not toxic compared to control (cell viability: 100%). Wound healing assay revealed that wound healing ability in each treated group was 51% to 61%. This was lower than wound healing area in the control. Using RT-PCR, inhibition of MMP-1/MMP-9 gene expression was examined. As a result, each group treated with fraction showed reduced expression of both MMP-1 and MMP-9 compared to tumor necrosis factor-α (TNF-α) treatment group. Effects on collagen biosynthesis were analyzed using a PICP ELISA Kit. The group in which Artemisia vulgaris was treated increased collagen synthesis from 141 to 262ng/mL compared to the control group. Three-dimensional cell culture revealed that each fraction could increase skin wall formation. These results suggest that each fractions has anti-aging and anti-wrinkle effects on the skin, indicating its suitability as a functional material.
S-397 60min glucose have more predictive value for incident type 2 diabetes than 120min glucose
( Ye Seul Jang ),( Jong Dai Kim ),( Dong Mi Im ),( Se Eun Park ),( Eun Jung Rhee ),( Cheul Young Park ),( Ki Won Oh ),( Sung Woo Park ),( Geun Yong Park ),( Won Young Lee ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
Objectives: To examine the value of 60min glucose during oral glucose tolerance test (OGTT) for predicting future diabetes compared to traditional prediabetes criteria 120min glucose in non-diabetic subjects.?Methods: From 2008 Jan to 2008 December, total 788 patient did OGTT. Of these, non-diabetes that have been followed-up during average 55±11 months. total 274 patients were enrolled in this study.?Results: During follow up, total 42 patients were progressed into diabetes (15.3%). The glucose peak time during OGTT is more later, DM progression rate is higher. DM progression rate is 7.5% in glucose peak time 30min group, 16.7% in 60 min group, and 29.4% in 90min group. Area under ROC curve (AROC) of 60 min glucose vs 120min glucose for predicting future diabetes were 0.748 vs 0.706. 140 mg/dL at 120min glucose, current prediabetes guideline have sensitivity 73.8% and specificity 50.0% for predicting future diabetes. 60min glucose 188mg/dL have sensitivity 76.2% and spepcificity 64.4%. Highest 120min glucose tertile have odds ratio (OR) 4.07 (95% CI 1.63-10.18) for future diabetes development. Highest 60min glucose tertile have OR 9.93 (95% CI 3.23-30.58) compared to lowest tertile. Among 0, 30, 60, 90, and 120min glucose, 60min glucose were most correlated with disposition index (insulinogenic index/HOMA-IR)?Conclusions: 60min glucose have higher predictive value for DM progression than 120 min glucose
Ye-Seul Park,Jian Lee,Hui-Yeong Seong,Hwa-Jeong Lee,Yong Eui Koo 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11
Nickel was a metal that can be present in products containing hydrogenated oils and fats because it is used as a catalyst in the curing process of vegetable oils such as mixed cooking oils, processed oils and fats, shortening, and margarine. In addition, since stainless steel is manufactured by making an alloy of iron with chromium, it can also be eluted from kitchen utensils. In particular, nickel can cause various toxic effects such as respiratory toxicity such as lung and nasal cancer and contact dermatitis when ingested in large amounts. In this study, an analytical method was established in order to determine nickel in confectionery, bread, and chocolate-based products in South Korea. Sample preparation of microwave-assisted acid digestion was carried out in an eco-friendly manner using diluted nitric acid with a single reaction chamber at high temperature and pressure. Residual carbon content and residual acidity were evaluated for digestion efficiency. Determination of Ni in 100 samples of processed food products was performed by ICP-MS analysis. The validation of the analytical method was investigated through some performance parameters including specificity, linearity, accuracy, precision, the limit of detection (LOD), and the limit of quantification (LOQ) according to the AOAC guideline. The recovery of Ni was confirmed with an average of 98.5% by using a certified reference substance (CRM) Baby food composite (NIST SRM2383a), and LOD and LOQ were values 0.009 mg/kg and 0.029 mg/kg, other validation items were also within the acceptable range of criteria.
Effect of Ovarian Extract on Oocyte Maturation and Early Embryonic Development in Pigs
Seul-Gi Yang(Seul-Gi Yang),Jae-Hun Choi(Jae-Hun Choi),Young-Seo Jo(Young-Seo Jo),Ye-Won Kim(Ye-Won Kim),Dong-Mok Lee(Dong-Mok Lee),Hyo-Jin Park(Hyo-Jin Park),Deog-Bon Koo(Deog-Bon Koo) 한국동물보건학회 2022 한국동물보건학회지 Vol.1 No.2
Various factors in the ovary are known to regulate oocyte maturation and hormone secretory functions; however, the effect of ovarian extract (OE) on oocyte maturation and embryonic development in pigs remains unknown. In this study, we first evaluated whether OE supplementation in the in vitro maturation (IVM) medium alters the oocyte maturation capacity by affecting glucose/amino acid metabolites, meiotic maturation, cumulus cell (CC) expansion, and antioxidants. Various OE concentrations (50, 100, 200, 500, and 5000 μg/mL) were included in the IVM medium. Only the oocytes treated with 100 μg/mL OE exhibited an improved meiotic maturation rate when compared with that of the other groups (non-treated group, 78.6 ± 3.0% vs. 100 μg/mL OE-treated group, 81.6 ± 4.3%); however, the difference was not significant. To observe the changes in glucose and amino acid metabolism in the OE-treated oocytes, we measured the amounts of diverse constituents (glucose, lactate, glutamine, and ammonia) in the IVM medium containing OE. Lactate and ammonia levels in the OE-treated group after 44 h of IVM were higher (p < 0.01) than those in the non-treated group. In addition, the expression of the CC expansion factors (Has2 and Tnfaip6) significantly increased (p < 0.05), whereas the mRNA expression levels of antioxidative enzymes (Sod1, Cat, and Gpx1) significantly diminished (p < 0.05) in the OE-treated group. Moreover, mature oocytes treated with 100 μg/mL OE demonstrated increased subsequent embryonic development rates after 144 h of IVM. Thus, the addition of OE in IVM mediums may improve oocyte maturation capacity which could enhance antioxidative enzyme activation, energy metabolism, and expression of the CC expansion factors in porcine oocytes.
Ye-seul Kim,Yeon-soo Park,Gyeong-cheol Cho,Ki-ho Park,Shin-Hyang Kim,Seung Yeon Baik,Cho Long Kim,Soo-yun Jung,Won-Hye Lee,Youn-young Choi,Seung-Hwan Lee,Kee-Hong Choi 대한신경정신의학회 2018 PSYCHIATRY INVESTIGATION Vol.15 No.11
Objective This study evaluated the psychometric properties of the Korean Anxiety Screening Assessment (K-ANX) developed for screening anxiety disorders. Methods Data from 613 participants were analyzed. The K-ANX was evaluated for reliability using Cronbach’s alpha, item-total correlation, and test information curve, and for validity using focus group interviews, factor analysis, correlational analysis, and item characteristics based on item response theory (IRT). The diagnostic sensitivity and specificity of the K-ANX were compared with those of the Beck Anxiety Inventory (BAI) and Generalized Anxiety Disorder 7-item scale (GAD-7). Results The K-ANX showed excellent internal consistency (α=0.97) and item-total coefficients (0.92–0.97), and a one-factor structure was suggested. All items were highly correlated with the total scores of the BAI, GAD-7, and Penn State Worry Questionnaire. IRT analysis indicated the K-ANX was most informative as a screening tool for anxiety disorders at the range between 0.8 and 1.6 (i.e., top 21.2 to 5.5 percentiles). Higher sensitivity (0.795) and specificity (0.937) for identifying anxiety disorders were observed in the K-ANX compared to the BAI and GAD-7. Conclusion The K-ANX is a reliable and valid measure to screen anxiety disorders in a Korean sample, with greater sensitivity and specificity than current measures of anxiety symptoms.