Methylation of methyltrichlorosilane with methyl chloride over active metals and activated carbon

Yanjun Liu,Yiqian Jiang,Weiguang Chen,Yutang Shen,Yonghai Feng,Lingqin Shen,Aili Wang,Tingshun Jiang,Zhanao Wu 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.12

Gas phase methylation of methyltrichlorosilane with methyl chloride to high-valued dimethyldichlorosilane was carried out by using metallic aluminum as a chlorine acceptor in the co-presence of activated carbon, tin, and zinc. The addition of activated carbon in metallic aluminum significantly enhanced the methylation of methyltrichlorosilane,and dimethyldichlorosilane was dominantly produced. Activated carbon played a catalyst role in the methylation reaction. When active metals, such as tin and zinc, were added in the mixture of aluminum and activated carbon,the active metals and activated carbon synergistically catalyzed the methylation of methyltrichlorosilane with methyl chloride toward the formation of dimethyldichlorosilane.

High-sensitivity detection for cantharidin by ratiometric fluorescent sensor based on molecularly imprinted nanoparticles of quantum dots

Jiang Ling,Wenqi Zhang,Zijia Cheng,Yanjun Ding 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.112 No.-

Cantharidin (CTD), an effective component of Chinese herbal medicine with unique efficacy, caused poisoningor death constantly for incorrect use. Therefore, there is an intense need for a reliable, selective,sensitive and low-costing analytical method for monitoring the concentration of CTD in biological samples. In this work, a ratiometric fluorescent sensor (CdTe@MIPs/CDs@NIPs) was successfully constructedfor selective determination of CTD by simply integrated the non-imprinted polymers coated CDs(CDs@NIPs) with cadmium telluride quantum dots coated with molecular imprinted polymers(CdTe@MIPs). CDs@NIPs were directly used as reference signal without addition of CTD templates andthe as-prepared CdTe@MIPs were response signal. With the CTD addition, the fluorescence intensity ofCdTe@MIPs decreased, while the fluorescence of CDs@NIPs remained approximately unchanged, resultinga detection limit as low as 0.15 nM. By spiked human blood samples, the ratiometric sensor was successfullyapplied to CTD detection with good recoveries of 96.12–107.40% and relative standard deviation(RSD) of 2.87–3.96%. Moreover, after centrifuging and re-eluting, the developed sensor could be effectivelyrecycled and reused five times. Significantly, the proposed platform with low-cost and highsensitivityopens a door towards the practical applications of ratiometric sensor and shows immensepotential in pursuing the sensitive, reliable and low-cost determination of poisons in forensic toxicologyanalysis.

Resequencing 302 wild and cultivated accessions identifies genes related to domestication and improvement in soybean

Zhou, Zhengkui,Jiang, Yu,Wang, Zheng,Gou, Zhiheng,Lyu, Jun,Li, Weiyu,Yu, Yanjun,Shu, Liping,Zhao, Yingjun,Ma, Yanming,Fang, Chao,Shen, Yanting,Liu, Tengfei,Li, Congcong,Li, Qing,Wu, Mian,Wang, Min,Wu, Nature Publishing Group, a division of Macmillan P 2015 Nature biotechnology Vol.33 No.4

Understanding soybean (Glycine max) domestication and improvement at a genetic level is important to inform future efforts to further improve a crop that provides the world's main source of oilseed. We detect 230 selective sweeps and 162 selected copy number variants by analysis of 302 resequenced wild, landrace and improved soybean accessions at >11× depth. A genome-wide association study using these new sequences reveals associations between 10 selected regions and 9 domestication or improvement traits, and identifies 13 previously uncharacterized loci for agronomic traits including oil content, plant height and pubescence form. Combined with previous quantitative trait loci (QTL) information, we find that, of the 230 selected regions, 96 correlate with reported oil QTLs and 21 contain fatty acid biosynthesis genes. Moreover, we observe that some traits and loci are associated with geographical regions, which shows that soybean populations are structured geographically. This study provides resources for genomics-enabled improvements in soybean breeding.

Functional Evaluation of Transplanted Kidneys with Reduced Field-of-View Diffusion-Weighted Imaging at 3T

Yuan Xie,Yanjun Li,Jiqiu Wen,Xue Li,Zhe Zhang,Jianrui Li,Yan’e Zhao,Peng Wang,Jun Zhang,Ying Tian,Long Jiang Zhang,Guang Ming Lu 대한영상의학회 2018 Korean Journal of Radiology Vol.19 No.2

Objective: To determine the feasibility of reduced field-of-view diffusion-weighted imaging (rFOV DWI) with multi-b values to detect functional variability in transplanted kidneys. Materials and Methods: Using a 3T MRI scanner, multi-b rFOV DWI of transplanted kidney or native kidney was performed in 40 renal transplantation recipients and 18 healthy volunteers. The patients were stratified, according to an estimated glomerular filtration rate (eGFR): Group 1, eGFR ≥ 60 mL/min/1.73 m2; Group 2, eGFR ≥ 30 mL/min/1.73 m2 and < 60 mL/min/1.73 m2; Group 3, eGFR < 30 mL/min/1.73 m2. Total apparent diffusion coefficient (ADCT), perfusion-free ADC (ADCD) and perfusion fraction (FP) of kidneys were calculated and compared among the four groups. Correlations between the imaging results and eGFR were assessed. Results: All volunteers had eGFR ≥ 60 mL/min/1.73 m2, while 16, 16, and 8 patients were included in Groups 1, 2, and 3, respectively. In the renal cortex, ADCT was higher in Group 1 ([1.65 ± 0.13] x 10-3 mm2/s) than Group 3 ([1.44 ± 0.11] x 10-3 mm2/s) (p < 0.05), and the inter-group differences of FP values were significant (all p < 0.05) (0.330 ± 0.024, 0.309 ± 0.019, 0.278 ± 0.033, and 0.250 ± 0.028 for control group, Groups 1, 2, and 3, respectively). Renal cortical ADCT, ADCD, FP, and renal medullary ADCT and FP correlated positively with eGFR (r = 0.596, 0.403, 0.711, 0.341, and 0.323, respectively; all p < 0.05). When using 0.278 as the cutoff value, renal cortical FP had a sensitivity of 97.1% and a specificity of 66.7% for predicting decreased renal function. Conclusion: Multi-b rFOV DWI presents transplanted kidneys with high resolution, which is a promising functional tool for non-invasively monitoring function of transplanted kidneys.

[BMIM]Cl-nAlCl3 ionic liquid-catalyzed redistribution reaction between methyltrichlorosilane and low-boiling residue to dimethyldichlorosilane

Aili Wang,Yiqian Jiang,Weiguang Chen,Yanjun Liu,Yutang Shen,Tingshun Jiang,Zhanao Wu,Hengbo Yin 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.1

Methyltrichlorosilane and low-boiling residue from the synthesis of methylchlorosilanes via the direct reaction of silicon and methyl chloride were effectively converted to high-valued dimethyldichlorosilane catalyzed by 1-butyl-3-methylimidazolium chloroaluminate, [BMIM]Cl-nAlCl3, ionic liquid catalysts. The yield of dimethyldichlorosilane reached 69.1% when the redistribution reaction between methyltrichlorosilane and low-boiling residue was catalyzed by [BMIM]Cl-6AlCl3 at a reaction temperature of 150 8C for 300 min. The conversion of methyltrichlorosilane was 87.8%. And the conversions of tetramethylsilane, methylhydrodichlorosilane, and dimethylhydrochlorosilane present in low-boiling residue were ca. 100%, respectively. The ionic liquids could be recycled efficiently. This research provided an eco-friendly and economical route for the treatment of methyltrichlorosilane and low-boiling residue, which were the by-products in the direct synthesis of methylchlorosilanes. The possible reaction route was also discussed.

Transcription factor Sp1 is necessary and functional in regulating expression of oncogene ZNF703

Xiaolin Liao,Yongjie Lu,Junbao Yang,Tao Kuang,Lilin Jiang,Yanjun Wang,Huiqun Kang,Bo Jiang,Xiaoli Zhou,Sheng He 한국유전학회 2017 Genes & Genomics Vol.39 No.10

Zinc finger protein 703 (ZNF703) is a putative oncogene in patients with the luminal B molecular subtype of breast cancer. Although the exact function of ZNF703 protein remains largely unknown, its expression and regulation have been implicated in several physiological and pathological processes. In the current study, for the first time, we identified and characterized the human ZNF703 gene promoter region. As a means of characterizing the transcription elements required for expression of ZNF703 protein at different stages, we cloned the promoter region of ZNF703 then created chimeric reporter plasmids for use in luciferase assays. A progressive deletion analysis of the ZNF703 gene’s 5′ and 3′ -flanking regions revealed that the core promoter is located in a 256-bp region ranging from nt-539 to nt-283. Next, we examined the effects of sitespecific mutations and treatment with mithramycin A to identify the functional Sp1 binding site, which was found to be located in a 447 bp region that ranged from nt-509 to nt-76, displayed the characteristics of a CpG island, and overlapped with the promoter region. In conclusion, our data suggest that ZNF703 transcription is regulated by transcription factor Sp1. This finding should facilitate future studies of the mechanism which regulates expression of this important gene.

Aqueous two-phase extraction to separate alpha-ketoglutaric acid from fermentation broth by combining crystallization

Xueqi Shi,Weiyi Su,Hongyu Zhang,Jing Fang,Ning Xu,Yanjun Jiang,Hao Li 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.115 No.-

Alpha-ketoglutaric acid (a-KG) is a crucial intermediate with broad application prospects. However, thedownstream separation of a-KG from fermentation broth with low concentration and complex componentshas become the bottleneck of large-scale production. In this study, a-KG was separated from theactual fermentation broth by aqueous two-phase system (ATPS) and crystallization. The optimal ATPScomposed of acetone/(NH4)2SO4 was selected by studying the phase diagram and a-KG distributionbehavior in different organic solvents and salts/sugars. Under the optimized conditions, the purity ofa-KG products from crude fermentation broth reached 98.08%, and the one-pass extraction efficiencywas 90.52%. The removal of cell debris and soluble proteins increased with the increase of acetone and(NH4)2SO4 concentrations, with the removal ratios reaching up to 99.44% and 86.54%. In addition,92.56% of the glucose was distributed in the aqueous phase. Although the a-KG fermentation broth ismore complex than the whole-cell biotransformation, impurities in the crude fermentation broth couldbe efficiently removed and a higher a-KG distribution coefficient was obtained. Meanwhile, the recovered(NH4)2SO4 could be reused for fermentation medium. This strategy provides an economical and effectivemethod combined with upstream biosynthesis to recover a-KG and other bioproducts from crude fermentationbroth.