The CREG1-FBXO27-LAMP2 axis alleviates diabetic cardiomyopathy by promoting autophagy in cardiomyocytes

Liu Dan,Xing Ruinan,Zhang Quanyu,Tian Xiaoxiang,Qi Yanping,Song Haixu,Liu Yanxia,Yu Haibo,Zhang Xiaolin,Jing Quanmin,Yan Chenghui,Han Yaling 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Autophagy plays an important role in the development of diabetic cardiomyopathy. Cellular repressor of E1A-stimulated genes 1 (CREG1) is an important myocardial protective factor. The aim of this study was to investigate the effects and mechanisms of CREG1 in diabetic cardiomyopathy. Male C57BL/6 J mice, Creg1 transgenic mice and cardiac-specific knockout mice were used to establish a type 2 diabetes model. Small animal ultrasound, Masson’s staining and western blotting were used to evaluate cardiac function, myocardial fibrosis and autophagy. Neonatal mouse cardiomyocytes (NMCMs) were stimulated with palmitate, and the effects of CREG1 on NMCMs autophagy were examined. CREG1 deficiency exacerbated cardiac dysfunction, cardiac hypertrophy and fibrosis in mice with diabetic cardiomyopathy, which was accompanied by exacerbated autophagy dysfunction. CREG1 overexpression improved cardiac function and ameliorated cardiac hypertrophy and fibrosis in diabetic cardiomyopathy by improving autophagy. CREG1 protein expression was decreased in palmitate-induced NMCMs. CREG1 knockdown exacerbated cardiomyocyte hypertrophy and inhibited autophagy. CREG1 overexpression inhibited cardiomyocyte hypertrophy and improved autophagy. LAMP2 overexpression reversed the effect of CREG1 knockdown on palmitate-induced inhibition of cardiomyocyte autophagy. CREG1 inhibited LAMP2 protein degradation by inhibiting the protein expression of F-box protein 27 (FBXO27). Our findings indicate new roles of CREG1 in the development of diabetic cardiomyopathy.

Facile and Effective Preparation of the Lotus Leaf-based Adsorbent by Exposing Cellulose Nanocrystal for Waste Water Treatment and Air Purification

Huiyu Yang,Ke Liu,Xin Liu,Yaling Wang,Keshuai Liu,Fengxiang Chen,Bo Deng,Weilin Xu 한국섬유공학회 2020 Fibers and polymers Vol.21 No.2

One substantial plant waste, lotus leave was fabricated into lotus leave powder with different diameters by ball milling. The surface morphology, chemical composition, particle size, porosity parameters, crystal structure, and wettabilityof lotus leaf powders (LLPs) were analyzed by scanning electron microscopy (SEM), high resolution transmission electron microscopy (HR-TEM), Fourier-transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), laser particle size analysis, N2 adsorption/desorption, X-ray diffraction (XRD) analysis, and water contact angle (WCA) analysis, respectively. The results showed that the micro- and nanoscale waxy papilla structures on the surface of the lotus leaf were gradually destroyed by mechanical forces along with extended balling time. Accompanied re-exposing of -OH affluent cellulose nanocrystals (CNCs) was proved by HR-TEM, XRD, and FTIR. FTIR also revealed a hydroxyl to carboxyl transition on the surface of CNCs. Both the re-exposure of CNCs and thereof chemical structure transition after ball millingmade the LLP a good candidate for pollutant adsorbent for dye, formaldehyde, and heavy metal ions.

Hydrogen sulfide ameliorates abdominal aorta coarctation-induced myocardial fibrosis by inhibiting pyroptosis through regulating eukaryotic translation initiation factor 2α phosphorylation and activating PI3K/AKT1 pathway

Yaling Li,Zhixiong Wu,Jiangping Hu,Gongli Liu,Hongming Hu,Fan Ouyang,Jun Yang The Korean Society of Pharmacology 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.4

This study aimed to assess the effects of exogenous hydrogen sulfide (H₂S) on abdominal aorta coarctation (AAC) induced myocardial fibrosis (MF) and autophagy in rats. Forty-four Sprague-Dawley rats were randomly divided into control group, AAC group, AAC + H₂S group, and H₂S control group. After a model of rats with AAC was built surgically, AAC + H₂S group and H₂S group were injected intraperitoneally with H₂S (100 µmol/kg) daily. The rats in the control group and the AAC group were injected with the same amount of PBS. We observed that H₂S can improve left ventricular function and the deposition of myocardial collagen fibers, inhibit pyroptosis, down-regulate the expression of P-eif2α in myocardial tissue, and inhibit cell autophagy by activating the phosphatidylinositol 3-kinase (PI3K)/AKT1 signaling pathway (p < 0.05). In addition, angiotensin II (1 µM) H9c2 cardiomyocytes were injured in vitro experiments, and it was also observed that pyroptosis was inhibited after H₂S (400 µmol/kg) intervention, the expression of P-eif2α in cardiomyocytes was significantly down-regulated, and the PI3K/AKT1 signaling pathway was activated at the same time. Therefore, increasing the expression of P-eif2α reverses the activation of the PI3K/AKT1 signaling pathway by H₂S. In conclusion, these findings suggest that exogenous H₂S can ameliorate MF in rats with AAC by inhibiting pyroptosis, and the mechanism may be associated with inhibiting the phosphorylation of eif2α and activating the PI3K/AKT1 signaling pathway to inhibit excessive cell autophagy.

A successive quadratic programming algorithm for SDP relaxation of the binary quadratic programing

Xuewen Mu,Sanyang Liu,Yaling Zhang 대한수학회 2005 대한수학회보 Vol.42 No.4

In this paper, we obtain a successive quadratic pro-gramming algorithm for solving the semidenite programming (SDP)relaxation of the binary quadratic programming. Combining witha randomized method of Goemans and Williamson, it provides anecient approximation for the binary quadratic programming. Fur-thermore, its convergence result is given. At last, We report somenumerical examples to compare our method with the interior-pointmethod on Maxcut problem.

Discovery of Endothelium and Mesenchymal Properties of Primo Vessels in the Mesentery

Ping, An,Zhendong, Su,Jingxing, Dai,Yaling, Liu,Bae, Kyung-Hee,Shiyun, Tan,Hesheng, Luo,Soh, Kwang-Sup,Ryu, Yeon Hee,Kim, Sungchul Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>Recent evidences demonstrated that endothelial-to-mesenchymal transition (EndMT) has a crucial role in cancer and is recognized as a unique source of cancer-associated fibroblasts (CAFs). Primo vascular system (PVS) is a new circulatory system which may play an important role in cancer metastasis and regeneration. In the current study, we applied previously established time-saving method to identify primo vessels and further investigated the immunocytochemical properties of primo vessels. Both primo vessels and primary primo vessel cells in the mesentery expressed endothelial markers and fibroblast markers. Double-labeling experiments demonstrated that endothelial and fibroblast markers are coexpressed in primo vessels. In addition, under the stimulation of TGF-<I>β</I>1 <I>in vitro,</I> primary primo vessel cells differentiated into fibroblasts. Therefore, we found that primo vessels in the mesentery had a transitional structure between endothelium and mesenchymal. This is a new finding of EndMT in normal postnatal animals.</P>

Experimental Study on the Damage of Steel Tubular Structural Components by Near-Field Detonations

Wanyue Wang,Shaobo Geng,Hua Wang,Wenqiang Li,Yaling Liu,Jianying Xue,Tingbian Zhan,Ying Gao 대한토목학회 2021 KSCE Journal of Civil Engineering Vol.25 No.2

This paper describes three blast-loading trials on three kinds of steel tubular structural components at the same explosive charge and standoff distance. Specimen one is a hollowsteel tube (HST), another is a HST wrapped with glass fibre-reinforced plastic (GFRP) with epoxy resin and the third is a HST infilled with concrete. The main objective of the trials is to investigate the effect of near-field detonations on circular steel tubular components. The experimental data, such as the overpressure time history, front local deformation, rear residual deflection and strain time history, are all recorded and collected. Analysis of the trial results shows that the experimental peak overpressure values of shot 1-3 are all larger than that of numerical simulation. The failure of these three specimens mainly experiences local damage, at the same time, the HST and HST with GFRP exhibit obvious global deformation. With the initiation point at one end of the cylinder explosive, both the maximum depth deformation on the front surface and the maximum residual deflection on the rear surface are all located on the side of the another end of the cylinder explosive. The blast-resistant approach by covering 10-layer GFRP on the surface of the HST can reduce the local damage, the global deformationand the dynamic strain value to certain extent, while the approach by infilling the steel tube with concrete can greatly decrease the local damage and the dynamic strain value and without any global deformation.

Define of Optimal Addition Period of Osteogenic Peptide to Accelerate the Osteogenic Differentiation of Human Pluripotent Stem Cells

Song Yameng,Li Hongjiao,Wang Zixuan,Shi Jiamin,Li Jing,Wang Lu,Liao Lingzi,Ma Shengqin,Zhang Yun,Liu Bin,Yang Yaling,Zhou Ping 한국조직공학과 재생의학회 2024 조직공학과 재생의학 Vol.21 No.2

Background: The addition of growth factiors is commonly applied to improve the osteogenic differentiation of stem cells. However, for human pluripotent stem cells (hPSCs), their complex differentiation processes result in the unknown effect at different stages. In this study, we focused on the widely used bone forming peptide-1 (BFP-1) and investigated the effect and mechanisms of its addition on the osteogenic induction of hPSCs as a function of the supplementation period. Methods: Monolayer-cultured hPSCs were cultured in osteogenic induction medium for 28 days, and the effect of BFP-1 peptide addition at varying weeks was examined. After differentiation for varying days (0, 7, 14, 21 and 28), the differentiation efficiency was determined by RT–PCR, flow cytometry, immunofluorescence, and alizarin red staining assays. Moreover, the expression of marker genes related to germ layers and epithelial-mesenchymal transition (EMT) was investigated at day 7. Results: Peptide treatment during the first week promoted the generation of mesoderm cells and mesenchymal-like cells from hiPSCs. Then, the upregulated expression of osteogenesis marker genes/proteins was detected in both hESCs and hiPSCs during subsequent inductions with BFP-1 peptide treatment. Fortunately, further experimental design confirmed that treating the BFP-1 peptide during 7–21 days showed even better performance for hESCs but was ineffective for hiPSCs. Conclusion: The differentiation efficiency of cells could be improved by determining the optimal treatment period. Our study has great value in maximizing the differentiation of hPSCs by adding osteogenesis peptides based on the revealed mechanisms and promoting the application of hPSCs in bone tissue regeneration. Background: The addition of growth factiors is commonly applied to improve the osteogenic differentiation of stem cells. However, for human pluripotent stem cells (hPSCs), their complex differentiation processes result in the unknown effect at different stages. In this study, we focused on the widely used bone forming peptide-1 (BFP-1) and investigated the effect and mechanisms of its addition on the osteogenic induction of hPSCs as a function of the supplementation period. Methods: Monolayer-cultured hPSCs were cultured in osteogenic induction medium for 28 days, and the effect of BFP-1 peptide addition at varying weeks was examined. After differentiation for varying days (0, 7, 14, 21 and 28), the differentiation efficiency was determined by RT–PCR, flow cytometry, immunofluorescence, and alizarin red staining assays. Moreover, the expression of marker genes related to germ layers and epithelial-mesenchymal transition (EMT) was investigated at day 7. Results: Peptide treatment during the first week promoted the generation of mesoderm cells and mesenchymal-like cells from hiPSCs. Then, the upregulated expression of osteogenesis marker genes/proteins was detected in both hESCs and hiPSCs during subsequent inductions with BFP-1 peptide treatment. Fortunately, further experimental design confirmed that treating the BFP-1 peptide during 7–21 days showed even better performance for hESCs but was ineffective for hiPSCs. Conclusion: The differentiation efficiency of cells could be improved by determining the optimal treatment period. Our study has great value in maximizing the differentiation of hPSCs by adding osteogenesis peptides based on the revealed mechanisms and promoting the application of hPSCs in bone tissue regeneration.