High-level extracellular production and characterization of Candida antarctica lipase B in Pichia pastoris

Eom, G.T.,Lee, S.H.,Song, B.K.,Chung, K.W.,Kim, Y.W.,Song, J.K. Society for Bioscience and Bioengineering, Japan ; 2013 Journal of bioscience and bioengineering Vol.116 No.2

The gene encoding lipase B from Candida antarctica (CalB) was expressed in Pichia pastoris after it was synthesized by the recursive PCR and cloned into the Pichia expression plasmid, pPICZαA. The CalB was successfully secreted in the recombinant P. pastoris strain X-33 with an apparent molecular weight of 34 kDa. For 140 h flask culture, the dry cell weight and the extracellular lipase activity reached at 5.4 g/l and 57.9 U/l toward p-nitrophenyl palmitate, respectively. When we performed the fed-batch fermentation using a methanol feeding strategy for 110 h, the dry cell weight and the extracellular lipase activity were increased to 135.7 g/l and 11,900 U/l; the CalB protein concentration was 1.18 g/l of culture supernatant. The characteristics of CalB recovered from the P. pastoris culture were compared with the commercial form of CalB produced in Aspergillus oryzae. The kinetic constants and specific activity, the effects of activity and stability on temperature and pH, the glycosylation extent, the degree of immobilization on macroporous resin and the yield of esterification reaction between oleic acid and n-butanol were almost identical to each other. Therefore, we successfully proved that the Pichia-based expression system for CalB in this study was industrially promising compared with one of the most efficient production systems.

중학교 성교육 지침과 실태 비교

곽예림,권경미,김유리,신지연,엄영아,오가영,윤미혜,윤예림,정자혜,최은아 이화여자대학교 간호과학대학 2010 이화간호학회지 Vol.- No.44

Nowadays, juvenile's sexual-related delinquency is increasing and their immature sexual behavior is on issue with this recent phenomenon. That is the reason people realize offering right sex education for them is important. So, we are going to study for trying to make an improvement in sex education field based on the fact that is practicing now at school. We will reference "소중한 성 바로알기" in this study which is published in 2008 for schoolteacher to compensate some limitations of former education material. This study was done from June 15 to July 20, 2009(Total : 36days) and aimed for surveying senior year students of middle school located in Seoul, S. Korea. We sampled 248 students by random from 8 classes of 4 schools. We analyzed final 209 samples(the other 39 samples were omitted for inappropriate response and return.) using SPSS WIN 12.0. The questionnaire includes 4 parts of relationship and psychosexual, physical growth and sexual health, social environment and sexual equality, and healthy family with marriage and it consists of 31 questions. We made this questionnaire base on the reference as we stated above. From this study we found the part of healthy family with marriage has not enough contents compared with the other 3 parts. And also we could see students don't feel satisfaction with one-off, one-sided sex education, and the education which is performed with only one single method. Therefore we make a judgement that middle school students have their needs toward sex education at school and those are considered as regular education by well-trained education provider, and various means for education such as case study, discussion, or role play. As well we think sex education for middle school students need to offer more about sexual equality and also alert the increasing possibilities of sexual commit in cyber space to adolescent.

Overcoming various comorbidities by G-CSF-primed unmanipulated BM SCT in adult patients with AML

Kim, H-J,Min, W-S,Cho, B-S,Eom, K-S,Kim, S-Y,Kim, Y-J,Lee, S,Min, C-K,Cho, S-G,Lee, J-W,Kim, C-C Macmillan Publishers Limited 2009 BONE MARROW TRANSPLANTATION -BASINGSTOKE- Vol.44 No.6

We examined whether the use of G-CSF-primed unmanipulated bone marrow (pBM) permits successful transplantation in patients with adverse comorbid pretransplantation conditions. A total of 33 patients at variable risk of AML undergoing allogeneic SCT from an HLA-identical sibling participated. The patients suffered from a variety of treatment-related sequelae before SCT and many cases also featured the presence of major organ dysfunction. The median follow-up duration of patients who were among the overall survivors was 58 months (range, 11−125 months). The median number of CD34<SUP>+</SUP> cells infused was 3.5 × 10<SUP>6</SUP> per kg (range, 0.8−15.6 × 10<SUP>6</SUP> per kg). The median number of days for recovery of ANC and platelet count without transfusion was 13 (range, 6−22) and 18 (range, 11−29) days, respectively. Four patients (12%) died due to nonrelapse causes and only one patient developed the same course of infectious complication as before SCT. No particular finding in the context of CMV infection or other post transplant complication was observed. The estimated 10-year overall survival rate was 68%. Our results suggest that the use of pBM allows successful engraftment without increasing complications in patients with various comorbidities before transplantation.

장애인 주간보호시설의 비대면 체육수업 운영 경험에 관한 연구

노승덕,이근호,박철승 한국특수체육학회 2022 한국특수체육학회지 Vol.30 No.2

The purpose of this study is to understand the improvement factors of non-face-to-face physical education classes in day care center used by adults with developmental disabilities in the COVID-19 era. Accordingly, in-depth interviews were conducted with 5 sports instructors for the disabled who are working at the G sports association for the disabled. Data collection was conducted from August 2021 to February 2022, and a triangulation method such as content review of research participants and expert meetings was implemented to secure the trustworthiness of the research. For the analysis of the collected data, the topics were selected as ‘problems of non-face-to-face physical education classes’, ‘contents of online classes in day care facilities for the disabled’, and ‘plans to improve online classes’ through an inductive content analysis method. The results of this study are as follows. First, the problems of non-face-to-face physical education classes were difficulty in online class preparation process, difficulty in online class process, and poor exercise environment in daycare center for the disabled. Second, the contents of online physical education classes at daycare center for the disabled were customized classes through program transformation and online cooperative classes with daycare center managers. Third, It was found that expansion of equipment and facilities for online classes, preparation of sports instructors for the disabled and manpower support system, establishment of an online physical education system for the disabled were found to be necessary to improve online physical education classes. Based on these results, in order to activate non-face-to-face physical education classes in daycare center for the disabled, improvement of the online physical education class environment, capability development of sports instructors for the disabled and cooperation with the manger in charge of daycare center, and preparation of an online physical education system for the disabled is presumed to be necessary. 본 연구는 코로나19 상황에서 성인 발달장애인들이 이용하는 주간보호시설에서의 비대면 체육수업의 개선 요인을 이해하는데 그 목적이 있다. 이를 위해 G장애인체육회에서 근무하고 있는 장애인스포츠지도사 5명을 대상으로 심층면담을 실시하였다. 자료 수집은 2021년 8월부터 2022년 2월까지 진행하였으며, 연구의 진실성을 확보하기 위해 연구 참여자의 내용검토, 전문가회의 등 삼각검증법을 시행하였다. 수집된 자료의 분석은 귀납적 내용분석 방법을 통해 ‘비대면 체육 수업의 문제점’, ‘장애인주간보호시설에서 온라인 수업내용’, ‘온라인 수업 개선방안’으로 주제를 선정하였다. 연구 결과는 첫째, 비대면 체육 수업의 문제점은 ‘온라인 수업 준비과정의 어려움’, ‘온라인 수업 진행과정 어려움’, ‘장애인주간보호시설의 열악한 운동환경’으로 나타났다. 둘째, 장애인주간보호시설에서 온라인 체육수업 내용은 ‘프로그램 변형을 통한 맞춤형 수업’, ‘시설 담당자와 온라인 협력수업’으로 나타났다. 셋째, 온라인 체육수업 개선방안으로 ‘온라인 수업을 위한 장비 및 시설 확충’, ‘지도자의 역량 및 인력지원 체계 마련’, ‘장애인을 위한 온라인 시스템 구축’이 필요한 것으로 나타났다. 이 결과로 보아 장애인 주간보호시설의 비대면 체육수업을 활성화를 위해서는 온라인 체육수업 환경의 개선, 지도자 역량 및 주간보호시설 담당자와의 협력, 장애인을 위한 온라인 체육수업 시스템이 마련되어야 할 것으로 사료된다.

화력발전소의 대기오염물질 배출계수 산정 연구

김대곤,엄윤성,홍지형,이석조,석광설,이대균,이은정,방선애 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.3

The main purpose of this study was to characterize the air pollutants emission factors in electric power plant (EPP) using fossil fuels. The electric power plant is a major air pollution source, thus knowing the emission characteristics of electric power plant is very important to develop a control strategy. The major air pollutants of concern from EPP stacks are particulate matter (PM), sulfur oxides (SOx), nitrogen oxides (NOx), carbon monoxide (CO) and heavy metals. Throughout the study. the following results arc estimated: - PM : 8.671E-05∼8.724E+01 PM emission (kg) per fuel burned (ton) - SOx: 4.749E-04∼7.877E+01 SOx emission (kg) per fuel burned (ton) - NOx : 1.578E-02∼9.857E+00 NOx emission (kg) per fuel burned (ton) - CO : 3.800E-04∼1.291E+00 CO emission (kg) per fuel burned (ton) - Hg : 1.220E+01∼3.108E+02 Hg emission (mg) per fuel burned (ton) From the statistical analysis by Wilcoxon signed ranks test between the emission factors of ours and U.S. EPA's. we can yielded that: p > 0.05.

염색체 핵형 분류를 위한 최적 패턴인식 시스템 개발에 관한 연구

남기곤,엄상희,장용훈,전계록,이권순 대한의료정보학회 1998 Healthcare Informatics Research Vol.4 No.1

Stabilization/solidification of mercury-contaminated waste ash using calcium sodium phosphate (CNP) and magnesium potassium phosphate (MKP) processes

Cho, J.H.,Eom, Y.,Lee, T.G. Elsevier Scientific Pub. Co 2014 Journal of hazardous materials Vol.278 No.-

This study examined the stabilization and solidification (S/S) of mercury (Hg)-contaminated waste ash generated from an industrial waste incinerator using chemically bonded phosphate ceramic (CBPC) technology. A magnesium potassium phosphate (MKP; MgKPO<SUB>4</SUB>.6H<SUB>2</SUB>O) ceramic, fabricated from MgO and KH<SUB>2</SUB>PO<SUB>4</SUB>, and a calcium sodium phosphate (CNP; CaNaPO<SUB>4</SUB>) ceramic, fabricated from CaO and Na<SUB>2</SUB>HPO<SUB>4</SUB>, were used as solidification binders in the CBPC process, and Na<SUB>2</SUB>S or FeS was added to each solidification binder to stabilize the Hg-contaminated waste ash. The S/S processes were conducted under various operating conditions (based on the solidification binder and stabilization reagent, stabilization reagent dosage, and waste loading ratio), and the performance characteristics of the S/S sample under each operating condition were compared, including the Hg leaching value and compressive strength. The Hg leaching value of untreated Hg-contaminated waste ash was 231.3μg/L, whereas the S/S samples treated using the MKP and CNP processes exhibited Hg leaching values below the universal treatment standard (UTS) limit (25μg/L). Although the compressive strengths of the S/S samples decreased as the sulfide dosage and waste loading ratio were increased, most of the S/S samples fabricated by the MKP and CNP processes exhibited good mechanical properties.

실온에서의 이중 장벽 구조의 Resonant Tunneling

강태원,홍치우,김순구,엄기석,정관수,정창섭 慶熙大學校 材料科學技術硏究所 1988 材料科學技術硏究論集 Vol.1 No.-

AlAs/GaAn/AlAs resonant tunneling structures were grown by molecular beam epitaxy (MBE). In this work we observe the occurrence of resonant tunneling through a GaAs/AlAs double barrier at room temperature. A peak to valley current ratio of 1.4 was observed. And we aware of a small deviation in current-voltage characteristics over the sample.

Clinical Outcome of Autologous Hematopoietic Cell Transplantation in Adult Patients with Acute Myeloid Leukemia: Who May Benefit from Autologous Hematopoietic Cell Transplantation?

Yoon, J.H.,Kim, H.J.,Park, S.S.,Jeon, Y.W.,Lee, S.E.,Cho, B.S.,Eom, K.S.,Kim, Y.J.,Lee, S.,Min, C.K.,Cho, S.G.,Kim, D.W.,Lee, J.W.,Min, W.S. AMERICAN SOCIETY FOR BLOOD AND MARROW 2017 BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION Vol.23 No.4

The role of autologous hematopoietic cell transplantation (auto-HCT) for postremission therapy of acute myeloid leukemia is yet to be elucidated. We retrospectively analyzed 240 patients treated with auto-HCT in first remission. All patients were treated with standard induction chemotherapy, and CD34<SUP>+</SUP>@?stem cells were collected at each cycle of consolidation. Stem cells were infused after total body irradiation (1200 cGy), cytarabine (9 g/m<SUP>2</SUP>), and melphalan (100@?mg/m<SUP>2</SUP>). Estimated 5-year overall survival, disease-free survival (DFS), cumulative incidence of relapse (CIR), and nonrelapse mortality were 58.4%, 55.3%, 38.8%, and 5.9%, respectively. We identified that poor-risk karyotype showed very poor outcome after auto-HCT, and then analyzed 85 patients with good to intermediate-risk molecular cytogenetics with available molecular study results and markers for minimal residual disease (MRD) such as WT1 and core-binding factor (CBF) associated MRD (ie, AML1/ETO and CBFβ/MYH11). Our data identified that old age, pre-HCT markers for MRD, and high post-HCT WT1, high dose of CD34<SUP>+</SUP>@?stem cell (≥4.5 x 10<SUP>6</SUP>/kg) infusion, and c-kit or FLT3-ITD mutations were associated with higher relapse rate and poor DFS. Using pre-HCT parameters, except for post-HCT WT1, multivariate analysis revealed that patients with young age (<40 years old), no adverse mutations, and limited dose of CD34<SUP>+</SUP>@?stem cells might be good candidate for auto-HCT (3-year DFS and CIR were 83.4% and 16.6%, respectively). Young patients with good- to intermediate-risk molecular cytogenetics may benefit from auto-HCT if stem cell dose is limited.

암에르고미터 운동시 여유심박수에서의 신체작업능력(PWC%HRR)을 이용한 최대산소섭취량 추정에 관한 연구

정성태,전태원,정덕조,정영수,엄우섭,이동기,윤호섭 한국운동과학회 2000 운동과학 Vol.9 No.1

암에르고미터 운동시 여유심박수에서의 신체작업능력(PWC%HRR)을 이용한 최대산소섭취량 측정에 관한 연구 운동과학. 제 9권 제 1호. (1)-(12), 2000. 본 연구의 목적은 암에르고미터 운동시 여유심박수에서의 신체작업능력(PWC%HRR)을 이용해 최대산소섭취량(O₂_(max))을 추정하는데 있어 그 타당성을 보는데 있다. 이를 위해 본 연구에서는 건강한20대 남자 대학생(21.1±2.77)20명이 참가 하였다. 이들의 안정시 심박수는 5분간 EKG로 측정되었고, 최대산소취량은 암에르고미터에서 50rpm의 속도로 20W에서 운동을 실시하고 매 3분 간격으로 10W씩 증가시켜 피험자가 탈진시까지 운동을 지속시켰다. 여유심박수 계산에 있어서 최대심박수는 수식(HRmax = 210 - age)으로 계산되었다. PWC%HRR의 계산은 점증부하 운동상에서 120≤R≥160의 범위에 발견된 HR과 O₂의 관계식(Y=bx+a)에 의하였다. 강도별 상대적 백분율 범위는 50∼85%사이였으며, 비교대상은 PWC%HRR, O₂R, O₂_(max)이었다. 그 결과 다음과 같은 결과를 얻었다 첫째, %HRR과 O₂R, O₂_(max)간의 회귀분석결과 %HRR은 O₂_(max)보다 O₂R에 가까웠다. 둘째, 각 강도별 PWC%HRR과 O₂R, O₂_(max)간에는 통계적으로 유의한 차이는 없었으나, PWC%HRR은 O₂_(max)보다 O₂R에 보다 가까웠다(P<.05). 셋째, O₂_(max)의 실측치와 PWC%HRR = O₂R에 의한 추정치 사이에는 모든 강도에서 통계적으로 유의한 차이가 없었으나, 상관관계는 PWC%HRR에서 가장 높았다(P<.05). 이상의 결과를 종합해 볼 때 암에르고미터 운동검사 과정에서 만족할 만한 O₂_(max) 발견하지 못했더라도 최소한 60%HRR에 해당하는 심박수가 발견될 때까지 운동을 수행하였다면 일련의 방식에 의해 O₂_(max)의 추정이 가능하다고 본다. The study on Estimation of maximal oxygun uptake through PWC%HRR during Arm Ergometer Exercise. Exercise Science, 9(1): 1-12, 2000. The purpose of this study was to evaluate the validity for estimation of maximal oxygen uptake through PWC%HRR during Arm Ergometer Exercise. The subjects of this study were 20 healthy males(21.2±2.77yrs) college students. Resting heart rate of subjects were recorded by EKG for 5min. the maximal oxygen uptake was determined until subjects were exhausted. The first stage was performed 50rpm, 20W, and then was increased by 10W every 3 minutes on the arm ergometer. HRmax was determined by a numerical formula of HRmax = 210-age. PWC%HRR was assessed by relative equation (Y=bX+a) of HR and V˙O₂ observed in range 120≤R≥160 during the exercise test. Range of relative percentage was between 50% and 85%, comparison factor was examined among PWC%HRR, % V˙O₂R and % V˙O₂_(max). The results of this study are as follows : 1) Result of the regression analysis among PWC%HRR, % V˙O₂R and % V˙O₂_(max), %HRR was equivalent to % V˙O₂R rather than V˙O₂_(max). 2) No significant difference was observed among PWC%HRR, % V˙O₂R and V˙O₂_(max) in the all intensity, But the PWC%HRR was closer to % V˙O₂R rather than V˙O₂_(max)(P<.05). 3) No significant difference was the actual V˙O₂_(max) and the estimation value by PWC%HRR = % V˙O₂R in the all intensity, and the correlation analysis was the most correlated at the estimation value by PWC%60HRR. It was concluded that if the satisfactory V˙O₂_(max) was not found in Arm Ergometer exercise test but heart rate corresponded to 60%HRR, V˙O₂_(max) can be estimated by a series of equation.